################################## # NO CUT ENZYMES ( 97 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AgeI Aor51HI ApaI AscI Asp718I AviII AvrII BanI BbeI BcgI BciVI BcnI BglI BglII BlnI BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BspDI BspEI BsrBI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I ClaI CpoI DrdI EagI EclXI Eco47III Eco52I EcoNI EcoO65I EcoT22I FseI FspI HaeII Hin1I HindIII HphI KasI KpnI KspI MamI MfeI MluI MroI MunI MvnI NaeI NarI NciI NgoMIV NheI NotI NruI NsiI NspV PacI PflMI PinAI PmeI PshAI Psp1406I PvuI RsrII SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI Sse8387I StuI Van91I XcmI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 74 enzymes) # ################################### AflII 2529 CTTAAG C^TTAAG AflIII 2325 ACATGT A^C[GA][CT]GT AhdI 1780 GACTTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 2321 GTGCAC G^TGCAC AlwNI 2300 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 2189 CCTCAGG CC^T[ACGT]AGG ApaLI 2321 GTGCAC G^TGCAC Asp700I 2671 GAATCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 1780 GACTTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 2840 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 2771 CTCGAG C^[CT]CG[GA]G BalI 324 TGGCCA TGG^CCA BbrPI 2450 CACGTG CAC^GTG BbsI 2175 GTCTTC GAAGAC(2/6) BbvCI 697 GCTGAGG CCTCAGC(-5/-2) BclI 1433 TGATCA T^GATCA BfrI 2529 CTTAAG C^TTAAG BlpI 1696 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1696 GCTCAGC GC^T[ACGT]AGC BpuAI 2175 GTCTTC GAAGAC(2/6) BseRI 1411 GAGGAG GAGGAG(10/8) BsiYI 681 CCTTGAAATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 681 CCTTGAAATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmI 285 GAATGC GAATGC(1/-1) BsoBI 2771 CTCGAG C^[CT]CG[GA]G BspHI 1602 TCATGA T^CATGA BspLU11I 2325 ACATGT A^CATGT BsrDI 912 CATTGC GCAATG(2/0) BsrFI 1548 ACCGGC [GA]^CCGG[CT] Bsu36I 2189 CCTCAGG CC^T[ACGT]AGG CelII 1696 GCTCAGC GC^T[ACGT]AGC CfoI 2506 GCGC GCG^C Cfr10I 1548 ACCGGC [GA]^CCGG[CT] DraII 513 GGGTCCT [GA]G^G[ACGT]CC[CT] DraIII 2315 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 324 TGGCCA [CT]^GGCC[GA] Eam1105I 1780 GACTTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 2189 CCTCAGG CC^T[ACGT]AGG EcoO109I 513 GGGTCCT [GA]G^G[ACGT]CC[CT] EcoRV 1102 GATATC GAT^ATC FauI 1148 CCCGC CCCGC(4/6) FbaI 1433 TGATCA T^GATCA HapII 1549 CCGG C^CGG HhaI 2506 GCGC GCG^C HinP1I 2506 GCGC G^CGC HincII 1000 GTTAAC GT[CT]^[GA]AC HindII 1000 GTTAAC GT[CT]^[GA]AC HpaI 1000 GTTAAC GTT^AAC HpaII 1549 CCGG C^CGG MluNI 324 TGGCCA TGG^CCA MscI 324 TGGCCA TGG^CCA MspA1I 695 CAGCTG C[AC]G^C[GT]G MspI 1549 CCGG C^CGG NdeI 439 CATATG CA^TATG PaeR7I 2771 CTCGAG C^TCGAG PflFI 2840 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 2450 CACGTG CAC^GTG PmlI 2450 CACGTG CAC^GTG PpuMI 513 GGGTCCT [GA]G^G[AT]CC[CT] PvuII 695 CAGCTG CAG^CTG RcaI 1602 TCATGA T^CATGA SacI 2253 GAGCTC GAGCT^C SapI 566 GAAGAGC GCTCTTC(1/4) ScaI 35 AGTACT AGT^ACT SnaBI 1584 TACGTA TAC^GTA SpeI 148 ACTAGT A^CTAGT SphI 597 GCATGC GCATG^C SwaI 504 ATTTAAAT ATTT^AAAT TaqI 2772 TCGA T^CGA Tth111I 2840 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 2772 TCGA T^CGA XbaI 1072 TCTAGA T^CTAGA XhoI 2771 CTCGAG C^TCGAG XmnI 2671 GAATCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AspHI 2253 GAGCTC G[AT]GC[AT]^C 2321 GTGCAC BamHI 21 GGATCC G^GATCC 2787 GGATCC BanII 558 GGGCTC G[GA]GC[CT]^C 2253 GAGCTC BpmI 131 CTCCAG CTGGAG(16/14) 2435 CTCCAG BsaAI 1584 TACGTA [CT]AC^GT[GA] 2450 CACGTG BsaI 1416 GAGACC GGTCTC(1/5) 1787 GGTCTC BsgI 518 CTGCAC GTGCAG(16/14) 1379 GTGCAG BsiHKAI 2253 GAGCTC G[AT]GC[AT]^C 2321 GTGCAC BsmAI 1416 GAGAC GTCTC(1/5) 1788 GTCTC BsmFI 2083 GGGAC GGGAC(10/14) 1899 GTCCC Bsp1407I 1254 TGTACA T^GTACA 1500 TGTACA BspMI 346 GCAGGT ACCTGC(4/8) 2737 GCAGGT BsrGI 1254 TGTACA T^GTACA 1500 TGTACA BstAPI 412 GCAAACAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2095 GCACATTATGC BstXI 781 CCACCATTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2835 CCATAGACTTGG DsaI 861 CCATGG C^C[GA][CT]GG 2648 CCATGG EcoRI 112 GAATTC G^AATTC 1767 GAATTC EcoT14I 861 CCATGG C^C[AT][AT]GG 2648 CCATGG HgaI 1066 GACGC GACGC(5/10) 1854 GCGTC NcoI 861 CCATGG C^CATGG 2648 CCATGG PleI 1009 GACTC GAGTC(4/5) 2887 GACTC PstI 602 CTGCAG CTGCA^G 2762 CTGCAG SfaNI 2831 GATGC GCATC(5/9) 1069 GCATC SmlI 2529 CTTAAG C^T[CT][GA]AG 2771 CTCGAG SspBI 1254 TGTACA T^GTACA 1500 TGTACA SspI 1591 AATATT AAT^ATT 1912 AATATT StyI 861 CCATGG C^C[AT][AT]GG 2648 CCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 60 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI AseI AsnI AvaII BbvI BfaI BmyI BsaJI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraI EarI EcoRII Fnu4HI FokI HaeIII HinfI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MvaI MwoI NdeII NlaIII NlaIV NspI PshBI RsaI Sau3AI Sau96I ScrFI SfcI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII