(Circular) (Six-base) MAPSORT of: pFN21AE3690.seq Check: 799 from: 1 to: 7729 pFN21AE3690 7729 bp With 182 enzymes: SgfI * March 28, 2012 13:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2342 4733 2342 Size: 2391 5338 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5539 278 Size: 53 83 186 936 4003 2468 Fragments arranged by size: 4003 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5072 1937 Size: 3135 4594 Acc65I G'GTAC_C Cuts at: 5050 5050 Size: 7729 AclI AA'CG_TT Cuts at: 1563 5856 6229 1563 Size: 4293 373 3063 Fragments arranged by size: 4293 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3008 4233 4722 4836 5792 7176 7444 1632 Size: 1376 1225 489 114 956 1384 268 1917 Fragments arranged by size: 1917 1384 1376 1225 956 489 268 114 AflII C'TTAA_G Cuts at: 820 1017 4534 820 Size: 197 3517 4015 Fragments arranged by size: 4015 3517 197 AflIII A'CryG_T Cuts at: 1203 4330 6628 7605 1203 Size: 3127 2298 977 1327 Fragments arranged by size: 3127 2298 1327 977 AgeI A'CCGG_T Cuts at: 6560 6560 Size: 7729 AhdI GACnn_n'nnGTC Cuts at: 3790 6458 3790 Size: 2668 5061 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7729 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4398 1184 Size: 3214 4515 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4480 4512 4480 Size: 32 7697 AlwNI CAG_nnn'CTG Cuts at: 4310 5472 7044 4310 Size: 1162 1572 4995 Fragments arranged by size: 4995 1572 1162 ApaLI G'TGCA_C Cuts at: 4326 5786 6942 4326 Size: 1460 1156 5113 Fragments arranged by size: 5113 1460 1156 ApoI r'AATT_y Cuts at: 1493 2074 2117 2642 2833 2874 3514 3772 4143 Size: 581 43 525 191 41 640 258 371 Cuts at: 4143 4877 5038 5264 5416 7525 1493 Size: 734 161 226 152 2109 1697 Fragments arranged by size: 2109 1697 734 640 581 525 371 258 226 191 161 152 43 41 AseI AT'TA_AT Cuts at: 160 1032 4506 5001 5018 6283 160 Size: 872 3474 495 17 1265 1606 Fragments arranged by size: 3474 1606 1265 872 495 17 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7729 AvaI C'yCGr_G Cuts at: 1870 1943 4776 5054 1870 Size: 73 2833 278 4545 Fragments arranged by size: 4545 2833 278 73 BamHI G'GATC_C Cuts at: 2026 4792 5059 5426 2026 Size: 2766 267 367 4329 Fragments arranged by size: 4329 2766 367 267 BanI G'GyrC_C Cuts at: 618 943 1160 1787 5050 6505 618 Size: 325 217 627 3263 1455 1842 Fragments arranged by size: 3263 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2567 4262 5048 1391 Size: 1176 1695 786 4072 Fragments arranged by size: 4072 1695 1176 786 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4173 928 Size: 971 2274 4484 Fragments arranged by size: 4484 2274 971 BbvCI CC'TCA_GC Cuts at: 2703 2703 Size: 7729 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5920 5954 1903 Size: 34 3983 34 3678 Fragments arranged by size: 3983 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5621 6831 5621 Size: 1210 6519 BclI T'GATC_A Cuts at: 1548 3438 1548 Size: 1890 5839 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6340 136 Size: 107 122 71 631 605 4668 1525 Fragments arranged by size: 4668 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7724 7724 Size: 7729 BlpI GC'TnA_GC Cuts at: 3702 5146 3702 Size: 1444 6285 Bme1580I G_kGCm'C Cuts at: 1790 4330 5790 6946 1790 Size: 2540 1460 1156 2573 Fragments arranged by size: 2573 2540 1460 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7729 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3090 5460 6418 Size: 416 53 83 399 24 1673 2370 958 Cuts at: 6418 442 Size: 1753 Fragments arranged by size: 2370 1753 1673 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7729 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2121 4425 6389 1239 Size: 882 2304 1964 2579 Fragments arranged by size: 2579 2304 1964 882 Bpu10I CC'TnA_GC Cuts at: 2703 4261 2703 Size: 1558 6171 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5854 6719 7017 5854 Size: 865 298 6566 Fragments arranged by size: 6566 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3415 3798 6392 882 Size: 617 1916 383 2594 2219 Fragments arranged by size: 2594 2219 1916 617 383 BsaAI yAC'GTr Cuts at: 493 1204 3591 4457 5520 493 Size: 711 2387 866 1063 2702 Fragments arranged by size: 2702 2387 1063 866 711 BsaBI GATnn'nnATC Cuts at: 7723 7723 Size: 7729 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5536 5918 6553 275 Size: 53 83 186 936 4003 382 635 1451 Fragments arranged by size: 4003 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6162 6560 6834 6981 7392 6162 Size: 398 274 147 411 6499 Fragments arranged by size: 6499 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3745 3775 1178 Size: 30 165 30 2342 30 5132 Fragments arranged by size: 5132 2342 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3431 1186 Size: 2245 5484 BseYI C'CCAG_C Cuts at: 6932 7428 6932 Size: 496 7233 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2508 3405 1163 Size: 573 772 897 5487 Fragments arranged by size: 5487 897 772 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5179 5940 6968 1540 Size: 275 186 3178 761 1028 2301 Fragments arranged by size: 3178 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4262 4330 5790 5875 6946 4262 Size: 68 1460 85 1071 5045 Fragments arranged by size: 5045 1460 1071 85 68 BsmI GAATG_Cn' Cuts at: 2296 5240 5333 2296 Size: 2944 93 4692 Fragments arranged by size: 4692 2944 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2567 4262 4330 5048 5790 5875 6946 Size: 399 777 1695 68 718 742 85 1071 Cuts at: 6946 1391 Size: 2174 Fragments arranged by size: 2174 1695 1071 777 742 718 399 85 68 BspEI T'CCGG_A Cuts at: 7392 7392 Size: 7729 BspHI T'CATG_A Cuts at: 3607 5618 7348 3607 Size: 2011 1730 3988 Fragments arranged by size: 3988 2011 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2342 4733 5084 844 Size: 1498 2391 351 3489 Fragments arranged by size: 3489 2391 1498 351 BsrBI CCG'CTC Cuts at: 1394 1823 5616 1394 Size: 429 3793 3507 Fragments arranged by size: 3793 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2916 6224 6398 65 Size: 1183 1668 3308 174 1396 Fragments arranged by size: 3308 1668 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3553 6373 6560 1700 Size: 110 1743 2820 187 2869 Fragments arranged by size: 2869 2820 1743 187 110 BsrGI T'GTAC_A Cuts at: 96 3259 3505 96 Size: 3163 246 4320 Fragments arranged by size: 4320 3163 246 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7729 BssSI C'ACGA_G Cuts at: 5789 6801 5789 Size: 1012 6717 BstAPI GCAn_nnn'nTGC Cuts at: 794 2423 4106 794 Size: 1629 1683 4417 Fragments arranged by size: 4417 1683 1629 BstBI TT'CG_AA Cuts at: 6533 6533 Size: 7729 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7729 BstXI CCAn_nnnn'nTGG Cuts at: 2793 4847 2793 Size: 2054 5675 BstYI r'GATC_y Cuts at: 1972 2026 2266 3290 4709 4792 5059 5426 5811 Size: 54 240 1024 1419 83 267 367 385 Cuts at: 5811 5828 7280 7366 7378 7724 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1419 1024 385 367 346 267 240 86 83 54 17 12 Bsu36I CC'TnA_GG Cuts at: 4195 4195 Size: 7729 BtgI C'CryG_G Cuts at: 513 1065 2866 4653 513 Size: 552 1801 1787 3589 Fragments arranged by size: 3589 1801 1787 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6990 Fragments arranged by size: 6990 683 56 BtsI GCAGTG_nn' Cuts at: 2720 3202 3393 4182 5245 6039 6059 2720 Size: 482 191 789 1063 794 20 4390 Fragments arranged by size: 4390 1063 794 789 482 191 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7729 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7694 DraI TTT'AAA Cuts at: 2337 2512 2667 3780 3810 5033 2337 Size: 175 155 1113 30 1223 5033 Fragments arranged by size: 5033 1223 1113 175 155 30 DraIII CAC_nnn'GTG Cuts at: 4325 4325 Size: 7729 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6736 809 Size: 507 5420 1802 Fragments arranged by size: 5420 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2329 5176 6065 Size: 54 1425 50 275 18 499 2847 889 Cuts at: 6065 7694 8 Size: 1629 43 Fragments arranged by size: 2847 1629 1425 889 499 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5176 1537 Size: 275 3364 4090 Fragments arranged by size: 4090 3364 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2566 2961 3113 3801 4014 5659 1353 Size: 1213 395 152 688 213 1645 3423 Fragments arranged by size: 3423 1645 1213 688 395 213 152 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6316 6690 6836 387 Size: 1064 138 4727 374 146 1280 Fragments arranged by size: 4727 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4260 4260 Size: 7729 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2121 3008 4233 4425 4722 4836 5792 Size: 393 489 887 1225 192 297 114 956 Cuts at: 5792 6389 7176 7444 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1225 956 887 787 597 489 393 297 268 192 114 EcoO109I rG'GnC_Cy Cuts at: 2519 2519 Size: 7729 EcoRI G'AATT_C Cuts at: 2117 3772 5038 5416 2117 Size: 1655 1266 378 4430 Fragments arranged by size: 4430 1655 1266 378 EcoRV GAT'ATC Cuts at: 3109 3109 Size: 7729 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3737 3905 3737 Size: 168 7561 FspI TGC'GCA Cuts at: 6235 6235 Size: 7729 HaeII r_GCGC'y Cuts at: 1678 6876 1678 Size: 5198 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3430 3462 3716 3745 3748 Size: 32 135 32 1858 32 254 29 3 Cuts at: 3748 3776 3777 3808 4457 4489 6370 6402 6444 Size: 28 1 31 649 32 1881 32 42 Cuts at: 6444 6476 1373 Size: 32 2626 Fragments arranged by size: 2626 1881 1858 649 254 135 42 32 32 32 32 32 32 31 29 28 3 1 HincII GTy'rAC Cuts at: 669 1657 1938 3007 5073 5319 669 Size: 988 281 1069 2066 246 3079 Fragments arranged by size: 3079 2066 1069 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7729 HpaI GTT'AAC Cuts at: 3007 5319 3007 Size: 2312 5417 KpnI G_GTAC'C Cuts at: 5054 5054 Size: 7729 MfeI C'AATT_G Cuts at: 5328 5328 Size: 7729 MluI A'CGCG_T Cuts at: 7605 7605 Size: 7729 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2120 2188 4739 6843 7027 1305 Size: 815 68 2551 2104 184 2007 Fragments arranged by size: 2551 2104 2007 815 184 68 MscI TGG'CCA Cuts at: 10 64 1489 1832 2331 10 Size: 54 1425 343 499 5408 Fragments arranged by size: 5408 1425 499 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3079 3322 3612 5687 6046 6205 518 Size: 648 1913 243 290 2075 359 159 2042 Fragments arranged by size: 2075 2042 1913 648 359 290 243 159 MspA1I CmG'CkG Cuts at: 1606 2702 5145 5822 6970 7215 1606 Size: 1096 2443 677 1148 245 2120 Fragments arranged by size: 2443 2120 1148 1096 677 245 NcoI C'CATG_G Cuts at: 513 1065 2866 4653 513 Size: 552 1801 1787 3589 Fragments arranged by size: 3589 1801 1787 552 NdeI CA'TA_TG Cuts at: 387 2445 387 Size: 2058 5671 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7729 NotI GC'GGCC_GC Cuts at: 5176 5176 Size: 7729 NspI r_CATG'y Cuts at: 1132 1229 2606 3136 3327 3683 4334 4385 5087 Size: 97 1377 530 191 356 651 51 702 Cuts at: 5087 6632 1132 Size: 1545 2229 Fragments arranged by size: 2229 1545 1377 702 651 530 356 191 97 51 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7729 PciI A'CATG_T Cuts at: 4330 6628 4330 Size: 2298 5431 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 1108 Size: 902 6827 PfoI T'CCnGG_A Cuts at: 2009 3666 2009 Size: 1657 6072 PmeI GTTT'AAAC Cuts at: 5033 5033 Size: 7729 PmlI CAC'GTG Cuts at: 4457 4457 Size: 7729 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4249 4281 5798 5830 7311 7343 4249 Size: 32 1517 32 1481 32 4635 Fragments arranged by size: 4635 1517 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2519 2519 Size: 7729 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7729 PsiI TTA'TAA Cuts at: 5299 5299 Size: 7729 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3543 3575 3543 Size: 32 7697 PstI C_TGCA'G Cuts at: 830 2611 4771 5081 830 Size: 1781 2160 310 3478 Fragments arranged by size: 3478 2160 1781 310 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7729 PvuII CAG'CTG Cuts at: 2702 2702 Size: 7729 SacI G_AGCT'C Cuts at: 4262 4262 Size: 7729 SalI G'TCGA_C Cuts at: 1936 5071 1936 Size: 3135 4594 SapI GCTCTTCn'nnn_ Cuts at: 2566 2566 Size: 7729 SbfI CC_TGCA'GG Cuts at: 5081 5081 Size: 7729 ScaI AGT'ACT Cuts at: 2042 5977 7689 2042 Size: 3935 1712 2082 Fragments arranged by size: 3935 2082 1712 SfcI C'TryA_G Cuts at: 826 1045 2587 2607 4767 5077 6212 6893 7084 Size: 219 1542 20 2160 310 1135 681 191 Cuts at: 7084 826 Size: 1471 Fragments arranged by size: 2160 1542 1471 1135 681 310 219 191 20 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7729 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7729 SmaI CCC'GGG Cuts at: 1872 5056 1872 Size: 3184 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4534 4776 5833 6734 6996 820 Size: 197 926 2591 242 1057 901 262 1553 Fragments arranged by size: 2591 1553 1057 926 901 262 242 197 SnaBI TAC'GTA Cuts at: 493 3591 5520 493 Size: 3098 1929 2702 Fragments arranged by size: 3098 2702 1929 SpeI A'CTAG_T Cuts at: 152 2153 152 Size: 2001 5728 SphI G_CATG'C Cuts at: 1132 2606 5087 1132 Size: 1474 2481 3774 Fragments arranged by size: 3774 2481 1474 SspI AAT'ATT Cuts at: 5 52 3598 3919 5653 5 Size: 47 3546 321 1734 2081 Fragments arranged by size: 3546 2081 1734 321 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7729 StyI C'CwwG_G Cuts at: 513 1065 2866 4653 5168 513 Size: 552 1801 1787 515 3074 Fragments arranged by size: 3074 1801 1787 552 515 SwaI ATTT'AAAT Cuts at: 2512 2512 Size: 7729 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2865 5756 5773 5926 6111 6556 325 Size: 1255 1285 2891 17 153 185 445 1498 Fragments arranged by size: 2891 1498 1285 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2040 2611 3183 Size: 274 80 33 51 1443 63 571 572 Cuts at: 3183 3259 3505 3718 5975 7687 96 Size: 76 246 213 2257 1712 138 Fragments arranged by size: 2257 1712 1443 572 571 274 246 213 138 80 76 63 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7689 7721 7689 Size: 32 7697 Tth111I GACn'n_nGTC Cuts at: 1316 4848 1316 Size: 3532 4197 XbaI T'CTAG_A Cuts at: 3077 5065 3077 Size: 1988 5741 XhoI C'TCGA_G Cuts at: 1943 4776 1943 Size: 2833 4896 XmaI C'CCGG_G Cuts at: 1870 5054 1870 Size: 3184 4545 XmnI GAAnn'nnTTC Cuts at: 4680 5858 4680 Size: 1178 6551 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5537 276 Size: 53 83 186 936 4003 2468 Fragments arranged by size: 4003 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspOMI PspXI RsrII SacII SanDI SexAI SfiI SfoI SrfI XcmI