Restriction map for pFN21AE3690
 (Linear) (Six-base) MAPSORT of: KIEE3690.seq  Check: 5259  from: 1  to: 3024



KIEE3690.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 16:39  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     337    2728    3024 
   Size:        337    2391     296

  Fragments arranged by size: 

                2391     337     296
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1003    2228    2717    2831    3024 
   Size:       1003    1225     489     114     193

  Fragments arranged by size: 

                1225    1003     489     193     114
 
AflII C'TTAA_G

Cuts at:      0    2529    3024 
   Size:       2529     495
 
AflIII A'CryG_T

Cuts at:      0    2325    3024 
   Size:       2325     699
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1785    3024 
   Size:       1785    1239
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2393    3024 
   Size:       2393     631
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2475    2507    3024 
   Size:       2475      32     517

  Fragments arranged by size: 

                2475     517      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    2305    3024 
   Size:       2305     719
 
ApaLI G'TGCA_C

Cuts at:      0    2321    3024 
   Size:       2321     703
 
ApoI r'AATT_y

Cuts at:      0      69     112     637     828     869    1509    1767    2138 
   Size:         69      43     525     191      41     640     258     371

Cuts at:   2138    2872    3024 
   Size:        734     152

  Fragments arranged by size: 

                 734     640     525     371     258     191     152      69
                  43      41
 
AseI AT'TA_AT

Cuts at:      0    2501    2996    3013    3024 
   Size:       2501     495      17      11

  Fragments arranged by size: 

                2501     495      17      11
 
AvaI C'yCGr_G

Cuts at:      0    2771    3024 
   Size:       2771     253
 
BamHI G'GATC_C

Cuts at:      0      21    2787    3024 
   Size:         21    2766     237

  Fragments arranged by size: 

                2766     237      21
 
BanII G_rGCy'C

Cuts at:      0     562    2257    3024 
   Size:        562    1695     767

  Fragments arranged by size: 

                1695     767     562
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    2168    3024 
   Size:       2168     856
 
BbvCI CC'TCA_GC

Cuts at:      0     698    3024 
   Size:        698    2326
 
BclI T'GATC_A

Cuts at:      0    1433    3024 
   Size:       1433    1591
 
BlpI GC'TnA_GC

Cuts at:      0    1697    3024 
   Size:       1697    1327
 
Bme1580I G_kGCm'C

Cuts at:      0    2325    3024 
   Size:       2325     699
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1085    3024 
   Size:       1085    1939
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     116    2420    3024 
   Size:        116    2304     604

  Fragments arranged by size: 

                2304     604     116
 
Bpu10I CC'TnA_GC

Cuts at:      0     698    2256    3024 
   Size:        698    1558     768

  Fragments arranged by size: 

                1558     768     698
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1410    1793    3024 
   Size:       1410     383    1231

  Fragments arranged by size: 

                1410    1231     383
 
BsaAI yAC'GTr

Cuts at:      0    1586    2452    3024 
   Size:       1586     866     572

  Fragments arranged by size: 

                1586     866     572
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1740    1770    3024 
   Size:       1740      30    1254

  Fragments arranged by size: 

                1740    1254      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1426    3024 
   Size:       1426    1598
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     503    1400    3024 
   Size:        503     897    1624

  Fragments arranged by size: 

                1624     897     503
 
BsiHKAI G_wGCw'C

Cuts at:      0    2257    2325    3024 
   Size:       2257      68     699

  Fragments arranged by size: 

                2257     699      68
 
BsmI GAATG_Cn'

Cuts at:      0     291    3024 
   Size:        291    2733
 
Bsp1286I G_dGCh'C

Cuts at:      0     562    2257    2325    3024 
   Size:        562    1695      68     699

  Fragments arranged by size: 

                1695     699     562      68
 
BspHI T'CATG_A

Cuts at:      0    1602    3024 
   Size:       1602    1422
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     337    2728    3024 
   Size:        337    2391     296

  Fragments arranged by size: 

                2391     337     296
 
BsrDI GCAATG_nn'

Cuts at:      0     911    3024 
   Size:        911    2113
 
BsrFI r'CCGG_y

Cuts at:      0    1548    3024 
   Size:       1548    1476
 
BsrGI T'GTAC_A

Cuts at:      0    1254    1500    3024 
   Size:       1254     246    1524

  Fragments arranged by size: 

                1524    1254     246
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     418    2101    3024 
   Size:        418    1683     923

  Fragments arranged by size: 

                1683     923     418
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     788    2842    3024 
   Size:        788    2054     182

  Fragments arranged by size: 

                2054     788     182
 
BstYI r'GATC_y

Cuts at:      0      21     261    1285    2704    2787    3024 
   Size:         21     240    1024    1419      83     237

  Fragments arranged by size: 

                1419    1024     240     237      83      21
 
Bsu36I CC'TnA_GG

Cuts at:      0    2190    3024 
   Size:       2190     834
 
BtgI C'CryG_G

Cuts at:      0     861    2648    3024 
   Size:        861    1787     376

  Fragments arranged by size: 

                1787     861     376
 
BtsI GCAGTG_nn'

Cuts at:      0     715    1197    1388    2177    3024 
   Size:        715     482     191     789     847

  Fragments arranged by size: 

                 847     789     715     482     191
 
DraI TTT'AAA

Cuts at:      0     332     507     662    1775    1805    3024 
   Size:        332     175     155    1113      30    1219

  Fragments arranged by size: 

                1219    1113     332     175     155      30
 
DraIII CAC_nnn'GTG

Cuts at:      0    2320    3024 
   Size:       2320     704
 
EaeI y'GGCC_r

Cuts at:      0     324    3024 
   Size:        324    2700
 
EarI CTCTTCn'nnn_

Cuts at:      0     561     956    1108    1796    2009    3024 
   Size:        561     395     152     688     213    1015

  Fragments arranged by size: 

                1015     688     561     395     213     152
 
EcoICRI GAG'CTC

Cuts at:      0    2255    3024 
   Size:       2255     769
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     116    1003    2228    2420    2717    2831    3024 
   Size:        116     887    1225     192     297     114     193

  Fragments arranged by size: 

                1225     887     297     193     192     116     114
 
EcoO109I rG'GnC_Cy

Cuts at:      0     514    3024 
   Size:        514    2510
 
EcoRI G'AATT_C

Cuts at:      0     112    1767    3024 
   Size:        112    1655    1257

  Fragments arranged by size: 

                1655    1257     112
 
EcoRV GAT'ATC

Cuts at:      0    1104    3024 
   Size:       1104    1920
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1732    1900    3024 
   Size:       1732     168    1124

  Fragments arranged by size: 

                1732    1124     168
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1425    1457    1711    1740    1743    1771    1772    1803 
   Size:       1425      32     254      29       3      28       1      31

Cuts at:   1803    2452    2484    3024 
   Size:        649      32     540

  Fragments arranged by size: 

                1425     649     540     254      32      32      31      29
                  28       3       1
 
HincII GTy'rAC

Cuts at:      0    1002    3024 
   Size:       1002    2022
 
HpaI GTT'AAC

Cuts at:      0    1002    3024 
   Size:       1002    2022
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     115     183    2734    3024 
   Size:        115      68    2551     290

  Fragments arranged by size: 

                2551     290     115      68
 
MscI TGG'CCA

Cuts at:      0     326    3024 
   Size:        326    2698
 
MslI CAynn'nnrTG

Cuts at:      0    1074    1317    1607    3024 
   Size:       1074     243     290    1417

  Fragments arranged by size: 

                1417    1074     290     243
 
MspA1I CmG'CkG

Cuts at:      0     697    3024 
   Size:        697    2327
 
NcoI C'CATG_G

Cuts at:      0     861    2648    3024 
   Size:        861    1787     376

  Fragments arranged by size: 

                1787     861     376
 
NdeI CA'TA_TG

Cuts at:      0     440    3024 
   Size:        440    2584
 
NspI r_CATG'y

Cuts at:      0     601    1131    1322    1678    2329    2380    3024 
   Size:        601     530     191     356     651      51     644

  Fragments arranged by size: 

                 651     644     601     530     356     191      51
 
PciI A'CATG_T

Cuts at:      0    2325    3024 
   Size:       2325     699
 
PfoI T'CCnGG_A

Cuts at:      0       4    1661    3024 
   Size:          4    1657    1363

  Fragments arranged by size: 

                1657    1363       4
 
PmlI CAC'GTG

Cuts at:      0    2452    3024 
   Size:       2452     572
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2244    2276    3024 
   Size:       2244      32     748

  Fragments arranged by size: 

                2244     748      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     514    3024 
   Size:        514    2510
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1538    1570    3024 
   Size:       1538      32    1454

  Fragments arranged by size: 

                1538    1454      32
 
PstI C_TGCA'G

Cuts at:      0     606    2766    3024 
   Size:        606    2160     258

  Fragments arranged by size: 

                2160     606     258
 
PvuII CAG'CTG

Cuts at:      0     697    3024 
   Size:        697    2327
 
SacI G_AGCT'C

Cuts at:      0    2257    3024 
   Size:       2257     767
 
SapI GCTCTTCn'nnn_

Cuts at:      0     561    3024 
   Size:        561    2463
 
ScaI AGT'ACT

Cuts at:      0      37    3024 
   Size:         37    2987
 
SfcI C'TryA_G

Cuts at:      0     582     602    2762    3024 
   Size:        582      20    2160     262

  Fragments arranged by size: 

                2160     582     262      20
 
SmlI C'TyrA_G

Cuts at:      0    2529    2771    3024 
   Size:       2529     242     253

  Fragments arranged by size: 

                2529     253     242
 
SnaBI TAC'GTA

Cuts at:      0    1586    3024 
   Size:       1586    1438
 
SpeI A'CTAG_T

Cuts at:      0     148    3024 
   Size:        148    2876
 
SphI G_CATG'C

Cuts at:      0     601    3024 
   Size:        601    2423
 
SspI AAT'ATT

Cuts at:      0    1593    1914    3024 
   Size:       1593     321    1110

  Fragments arranged by size: 

                1593    1110     321
 
StyI C'CwwG_G

Cuts at:      0     861    2648    3024 
   Size:        861    1787     376

  Fragments arranged by size: 

                1787     861     376
 
SwaI ATTT'AAAT

Cuts at:      0     507    3024 
   Size:        507    2517
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     860    3024 
   Size:        860    2164
 
TatI w'GTAC_w

Cuts at:      0      35     606    1178    1254    1500    1713    3024 
   Size:         35     571     572      76     246     213    1311

  Fragments arranged by size: 

                1311     572     571     246     213      76      35
 
Tth111I GACn'n_nGTC

Cuts at:      0    2843    3024 
   Size:       2843     181
 
XbaI T'CTAG_A

Cuts at:      0    1072    3024 
   Size:       1072    1952
 
XhoI C'TCGA_G

Cuts at:      0    2771    3024 
   Size:       2771     253
 
XmnI GAAnn'nnTTC

Cuts at:      0    2675    3024 
   Size:       2675     349
 

 Enzymes that do cut:

     AarI     AcuI    AflII   AflIII     AhdI     AlfI     AloI    AlwNI
    ApaLI     ApoI     AseI     AvaI    BamHI    BanII     BbsI    BbvCI
     BclI     BlpI Bme1580I     BmrI     BpmI   Bpu10I     BsaI    BsaAI
    BsaXI    BseRI     BsgI  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI
    BsrDI    BsrFI    BsrGI   BstAPI    BstXI    BstYI   Bsu36I     BtgI
     BtsI     DraI   DraIII     EaeI     EarI  EcoICRI  Eco57MI EcoO109I
    EcoRI    EcoRV     FalI    Hin4I   HincII     HpaI     MmeI     MscI
     MslI   MspA1I     NcoI     NdeI     NspI     PciI     PfoI     PmlI
     PpiI    PpuMI     PsrI     PstI    PvuII     SacI     SapI     ScaI
     SfcI     SmlI    SnaBI     SpeI     SphI     SspI     StyI     SwaI
    TaqII     TatI  Tth111I     XbaI     XhoI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI     AgeI     AleI     ApaI
     AscI    AsiSI    AvrII     BaeI     BanI     BbeI     BcgI    BciVI
    BfrBI     BglI    BglII    BmgBI     BmtI     BplI    BpuEI    BsaBI
    BsaHI    BsaWI    BseYI    BsiEI    BsiWI    BsmBI    BspEI    BsrBI
   BssHII    BssSI    BstBI   BstEII  BstZ17I    BtgZI     ClaI    CspCI
     DrdI     EagI     EciI    EcoNI     FseI     FspI    FspAI    HaeII
  HindIII     KasI     KpnI     MfeI     MluI     NaeI     NarI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI     PmeI
    PshAI     PsiI   PspOMI    PspXI     PvuI    RsrII    SacII     SalI
    SanDI     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SrfI     StuI     TstI     XcmI     XmaI     ZraI
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