Restriction map for
pFN21AE2433
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# NO CUT ENZYMES ( 104 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AgeI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspI AvaI AviII
AvrII BbeI BbrPI BbvCI BlnI
BsaHI BsaWI BseAI BsiEI BsiWI
BsmBI BsoBI BspDI BspEI BspMI
BsrBI BsrFI BssHII Bst1107I BstEII
BstPI BstUI BstZ17I Bsu36I Cfr10I
ClaI CpoI DraIII DrdI EagI
EclXI Eco47III Eco52I Eco81I EcoO65I
FseI FspI HaeII Hin1I HindIII
HpaI KasI KpnI KspI MluI
MroI MvnI NaeI NarI NgoMIV
NotI NruI PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RsrII SacII SalI SbfI SfiI
SfoI SgrAI SmaI SnaBI SpeI
SphI Sse8387I StuI SwaI Tth111I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AflII 687 CTTAAG C^TTAAG
AhdI 702 GACACCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 3754 CAGGGTCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 1141 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 702 GACACCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 2120 TGGCCA TGG^CCA
BamHI 762 GGATCC G^GATCC
BanI 625 GGTGCC G^G[CT][GA]CC
BcgI 2094 GCAGAAAGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 687 CTTAAG C^TTAAG
BlpI 313 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 313 GCTGAGC GC^T[ACGT]AGC
BsaAI 160 TACGTG [CT]AC^GT[GA]
BstBI 1271 TTCGAA TT^CGAA
CelII 313 GCTGAGC GC^T[ACGT]AGC
CfoI 848 GCGC GCG^C
DraII 3704 AGGGCCT [GA]G^G[ACGT]CC[CT]
DsaI 840 CCATGG C^C[GA][CT]GG
EaeI 2120 TGGCCA [CT]^GGCC[GA]
Eam1105I 702 GACACCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 3704 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoRI 2326 GAATTC G^AATTC
EcoRV 248 GATATC GAT^ATC
EcoT22I 3016 ATGCAT ATGCA^T
FauI 787 GCGGG CCCGC(4/6)
HhaI 848 GCGC GCG^C
HinP1I 848 GCGC G^CGC
MaeII 161 ACGT A^CGT
MluNI 2120 TGGCCA TGG^CCA
MscI 2120 TGGCCA TGG^CCA
NcoI 840 CCATGG C^CATGG
NdeI 1706 CATATG CA^TATG
NheI 513 GCTAGC G^CTAGC
NsiI 3016 ATGCAT ATGCA^T
NspV 1271 TTCGAA TT^CGAA
PflMI 835 CCAGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 3190 GAGCTC GAGCT^C
SapI 1468 GAAGAGC GCTCTTC(1/4)
ScaI 3381 AGTACT AGT^ACT
SexAI 705 ACCAGGT A^CC[AT]GGT
SfuI 1271 TTCGAA TT^CGAA
SspI 3743 AATATT AAT^ATT
Van91I 835 CCAGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 1141 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AflIII 3364 ACATGT A^C[GA][CT]GT
3581 ACATGT
BanII 3190 GAGCTC G[GA]GC[CT]^C
3695 GAGCCC
BbsI 420 GAAGAC GAAGAC(2/6)
2886 GAAGAC
BclI 612 TGATCA T^GATCA
2652 TGATCA
BcnI 342 CCGGG CC^[GC]GG
3773 CCCGG
BglI 256 GCCCCTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
839 GCCATGGAGGC
BglII 2792 AGATCT A^GATCT
3042 AGATCT
BpuAI 420 GAAGAC GAAGAC(2/6)
2886 GAAGAC
BsaBI 2307 GATGTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2647 GATATTGATC
BsgI 2534 GTGCAG GTGCAG(16/14)
3566 GTGCAG
BspHI 2882 TCATGA T^CATGA
3270 TCATGA
BspLU11I 3364 ACATGT A^CATGT
3581 ACATGT
BssSI 1568 CTCGTG CACGAG(-5/-1)
1723 CTCGTG
BstAPI 1801 GCAAAGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2275 GCAGAATATGC
Cac8I 513 GCTAGC GC[ACGT]^[ACGT]GC
1974 GCAAGC
EarI 1468 GAAGAG CTCTTC(1/4)
2950 GAAGAG
EcoNI 802 CCTGGACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1108 CCTACTCCAGG
EcoT14I 840 CCATGG C^C[AT][AT]GG
2132 CCTTGG
FbaI 612 TGATCA T^GATCA
2652 TGATCA
HapII 342 CCGG C^CGG
3774 CCGG
HincII 288 GTCAAC GT[CT]^[GA]AC
3232 GTCAAC
HindII 288 GTCAAC GT[CT]^[GA]AC
3232 GTCAAC
HpaII 342 CCGG C^CGG
3774 CCGG
Ksp632I 1468 GAAGAG CTCTTC(1/4)
2950 GAAGAG
MamI 2307 GATGTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2647 GATATTGATC
MspI 342 CCGG C^CGG
3774 CCGG
NciI 342 CCGGG CC^[GC]GG
3773 CCCGG
RcaI 2882 TCATGA T^CATGA
3270 TCATGA
StyI 840 CCATGG C^C[AT][AT]GG
2132 CCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BbvI BciVI
BfaI BmyI BpmI BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I Bsp1407I BsrDI
BsrGI BsrI BssKI BstF5I BstNI
BstXI BstYI Cfr13I DdeI DpnII
DraI EcoRII Fnu4HI FokI HaeIII
HgaI HinfI HphI ItaI MaeI
MaeIII MboI MboII MfeI MflI
MnlI MseI MslI MspA1I MunI
MvaI MwoI NdeII NlaIII NlaIV
NspI PleI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII