(Circular) (Six-base) MAPSORT of: pFN21AE2433.seq Check: 5974 from: 1 to: 8617 pFN21AE2433 8617 bp With 182 enzymes: SgfI * February 22, 2013 16:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6427 278 Size: 53 83 186 936 4891 2468 Fragments arranged by size: 4891 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5960 1937 Size: 4023 4594 Acc65I G'GTAC_C Cuts at: 5938 5938 Size: 8617 AclI AA'CG_TT Cuts at: 1563 6744 7117 1563 Size: 5181 373 3063 Fragments arranged by size: 5181 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3137 3369 4164 4391 4986 5015 6680 8064 Size: 1505 232 795 227 595 29 1665 1384 Cuts at: 8064 8332 1632 Size: 268 1917 Fragments arranged by size: 1917 1665 1505 1384 795 595 268 232 227 29 AflII C'TTAA_G Cuts at: 820 1017 2692 820 Size: 197 1675 6745 Fragments arranged by size: 6745 1675 197 AflIII A'CryG_T Cuts at: 1203 5369 5586 7516 8493 1203 Size: 4166 217 1930 977 1327 Fragments arranged by size: 4166 1930 1327 977 217 AgeI A'CCGG_T Cuts at: 7448 7448 Size: 8617 AhdI GACnn_n'nnGTC Cuts at: 2712 7346 2712 Size: 4634 3983 AleI CACnn'nnGTG Cuts at: 1166 4926 1166 Size: 3760 4857 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8617 AlwNI CAG_nnn'CTG Cuts at: 5764 6360 7932 5764 Size: 596 1572 6449 Fragments arranged by size: 6449 1572 596 ApaLI G'TGCA_C Cuts at: 6674 7830 6674 Size: 1156 7461 ApoI r'AATT_y Cuts at: 1493 2055 2130 3578 4331 5805 5926 6152 6304 Size: 562 75 1448 753 1474 121 226 152 Cuts at: 6304 8413 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1474 1448 753 562 226 152 121 75 AseI AT'TA_AT Cuts at: 160 1032 7171 160 Size: 872 6139 1606 Fragments arranged by size: 6139 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8617 AvaI C'yCGr_G Cuts at: 1870 1943 5942 1870 Size: 73 3999 4545 Fragments arranged by size: 4545 3999 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4500 4533 4500 Size: 33 8584 BamHI G'GATC_C Cuts at: 2767 5947 6314 2767 Size: 3180 367 5070 Fragments arranged by size: 5070 3180 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2630 5938 7393 618 Size: 325 217 627 843 3308 1455 1842 Fragments arranged by size: 3308 1842 1455 843 627 325 217 BanII G_rGCy'C Cuts at: 1391 5199 5704 5936 1391 Size: 3808 505 232 4072 Fragments arranged by size: 4072 3808 505 232 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2432 4898 928 Size: 971 533 2466 4647 Fragments arranged by size: 4647 2466 971 533 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4088 4122 6808 6842 1903 Size: 34 2151 34 2686 34 3678 Fragments arranged by size: 3678 2686 2151 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2156 3672 4217 6509 7719 2156 Size: 1516 545 2292 1210 3054 Fragments arranged by size: 3054 2292 1516 1210 545 BclI T'GATC_A Cuts at: 1548 2617 4657 1548 Size: 1069 2040 5508 Fragments arranged by size: 5508 2040 1069 BfrBI ATG'CAT Cuts at: 5023 5023 Size: 8617 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2267 2850 7228 Size: 107 122 71 631 605 595 583 4378 Cuts at: 7228 136 Size: 1525 Fragments arranged by size: 4378 1525 631 605 595 583 122 107 71 BglII A'GATC_T Cuts at: 4797 5047 8612 4797 Size: 250 3565 4802 Fragments arranged by size: 4802 3565 250 BlpI GC'TnA_GC Cuts at: 2319 6034 2319 Size: 3715 4902 Bme1580I G_kGCm'C Cuts at: 1790 6678 7834 1790 Size: 4888 1156 2573 Fragments arranged by size: 4888 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8617 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 6348 7306 442 Size: 416 53 83 399 24 4931 958 1753 Fragments arranged by size: 4931 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2522 1056 Size: 1466 7151 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2117 2928 3156 2117 Size: 811 228 7578 Fragments arranged by size: 7578 811 228 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2559 2760 3012 3039 3102 3240 3267 7277 Size: 1320 201 252 27 63 138 27 4010 Cuts at: 7277 1239 Size: 2579 Fragments arranged by size: 4010 2579 1320 252 201 138 63 27 27 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2073 3656 6742 7607 7905 2073 Size: 1583 3086 865 298 2785 Fragments arranged by size: 3086 2785 1583 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2663 3838 5538 7280 882 Size: 617 1164 1175 1700 1742 2219 Fragments arranged by size: 2219 1742 1700 1175 1164 617 BsaAI yAC'GTr Cuts at: 493 1204 2167 6408 493 Size: 711 963 4241 2702 Fragments arranged by size: 4241 2702 963 711 BsaBI GATnn'nnATC Cuts at: 4316 4656 8611 4316 Size: 340 3955 4322 Fragments arranged by size: 4322 3955 340 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 6424 6806 7441 275 Size: 53 83 186 936 4891 382 635 1451 Fragments arranged by size: 4891 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 7050 7448 7722 7869 8280 7050 Size: 398 274 147 411 7387 Fragments arranged by size: 7387 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2971 3001 3100 3130 3289 Size: 30 165 30 1568 30 99 30 159 Cuts at: 3289 3319 1178 Size: 30 6476 Fragments arranged by size: 6476 1568 165 159 99 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2934 3243 5664 1186 Size: 1748 309 2421 4139 Fragments arranged by size: 4139 2421 1748 309 BseYI C'CCAG_C Cuts at: 2574 5773 7820 8316 2574 Size: 3199 2047 496 2875 Fragments arranged by size: 3199 2875 2047 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4560 5592 1163 Size: 573 2824 1032 4188 Fragments arranged by size: 4188 2824 1032 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6067 6828 7856 1540 Size: 275 186 4066 761 1028 2301 Fragments arranged by size: 4066 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2325 2869 4993 5199 6678 6763 7834 2325 Size: 544 2124 206 1479 85 1071 3108 Fragments arranged by size: 3108 2124 1479 1071 544 206 85 BsmI GAATG_Cn' Cuts at: 2452 3924 4980 6128 6221 2452 Size: 1472 1056 1148 93 4848 Fragments arranged by size: 4848 1472 1148 1056 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2325 2869 4993 5199 5704 5936 6678 Size: 399 535 544 2124 206 505 232 742 Cuts at: 6678 6763 7834 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 2124 1071 742 544 535 505 399 232 206 85 BspEI T'CCGG_A Cuts at: 8280 8280 Size: 8617 BspHI T'CATG_A Cuts at: 4887 5275 6506 8236 4887 Size: 388 1231 1730 5268 Fragments arranged by size: 5268 1730 1231 388 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5972 844 Size: 5128 3489 BsrBI CCG'CTC Cuts at: 1394 1823 6504 1394 Size: 429 4681 3507 Fragments arranged by size: 4681 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2270 3548 4128 7112 7286 65 Size: 1183 1022 1278 580 2984 174 1396 Fragments arranged by size: 2984 1396 1278 1183 1022 580 174 BsrFI r'CCGG_y Cuts at: 1700 1810 7261 7448 1700 Size: 110 5451 187 2869 Fragments arranged by size: 5451 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3684 4006 5551 96 Size: 3588 322 1545 3162 Fragments arranged by size: 3588 3162 1545 322 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8617 BssSI C'ACGA_G Cuts at: 3573 3728 6677 7689 3573 Size: 155 2949 1012 4501 Fragments arranged by size: 4501 2949 1012 155 BstAPI GCAn_nnn'nTGC Cuts at: 794 3812 4286 794 Size: 3018 474 5125 Fragments arranged by size: 5125 3018 474 BstBI TT'CG_AA Cuts at: 3277 7421 3277 Size: 4144 4473 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8617 BstXI CCAn_nnnn'nTGG Cuts at: 3103 3386 4564 3103 Size: 283 1178 7156 Fragments arranged by size: 7156 1178 283 BstYI r'GATC_y Cuts at: 1972 2767 3723 4797 5047 5947 6314 6699 6716 Size: 795 956 1074 250 900 367 385 17 Cuts at: 6716 8168 8254 8266 8612 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1074 956 900 795 385 367 346 250 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2845 513 Size: 552 939 841 6285 Fragments arranged by size: 6285 939 841 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7878 Fragments arranged by size: 7878 683 56 BtsI GCAGTG_nn' Cuts at: 5580 6133 6927 6947 5580 Size: 553 794 20 7250 Fragments arranged by size: 7250 794 553 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8617 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8582 DraI TTT'AAA Cuts at: 3405 3510 4450 4824 5921 3405 Size: 105 940 374 1097 6101 Fragments arranged by size: 6101 1097 940 374 105 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7624 809 Size: 507 6308 1802 Fragments arranged by size: 6308 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4125 6064 6953 Size: 54 1425 50 275 18 2295 1939 889 Cuts at: 6953 8582 8 Size: 1629 43 Fragments arranged by size: 2295 1939 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6064 1537 Size: 275 4252 4090 Fragments arranged by size: 4252 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3468 4950 6547 1353 Size: 2115 1482 1597 3423 Fragments arranged by size: 3423 2115 1597 1482 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2389 4199 7204 7578 7724 387 Size: 1064 138 800 1810 3005 374 146 1280 Fragments arranged by size: 3005 1810 1280 1064 800 374 146 138 EcoICRI GAG'CTC Cuts at: 5197 5197 Size: 8617 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2559 2760 3012 3039 3102 3137 3240 Size: 393 927 201 252 27 63 35 103 Cuts at: 3240 3267 3369 4164 4391 4986 5015 6680 7277 Size: 27 102 795 227 595 29 1665 597 Cuts at: 7277 8064 8332 1239 Size: 787 268 1524 Fragments arranged by size: 1665 1524 927 795 787 597 595 393 268 252 227 201 103 102 63 35 29 27 27 EcoNI CCTnn'n_nnAGG Cuts at: 2811 3117 2811 Size: 306 8311 EcoO109I rG'GnC_Cy Cuts at: 5710 5710 Size: 8617 EcoRI G'AATT_C Cuts at: 4331 5926 6304 4331 Size: 1595 378 6644 Fragments arranged by size: 6644 1595 378 EcoRV GAT'ATC Cuts at: 2255 2255 Size: 8617 FspI TGC'GCA Cuts at: 7123 7123 Size: 8617 HaeII r_GCGC'y Cuts at: 1678 7764 1678 Size: 6086 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2201 2233 2419 2451 2698 Size: 32 135 32 629 32 186 32 247 Cuts at: 2698 2730 3289 3321 3368 3400 3598 3630 4423 Size: 32 559 32 47 32 198 32 793 Cuts at: 4423 4455 5220 5252 5659 5691 7258 7290 7332 Size: 32 765 32 407 32 1567 32 42 Cuts at: 7332 7364 1373 Size: 32 2626 Fragments arranged by size: 2626 1567 793 765 629 559 407 247 198 186 135 47 42 32 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2295 5239 5961 6207 669 Size: 988 281 357 2944 722 246 3079 Fragments arranged by size: 3079 2944 988 722 357 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8617 HpaI GTT'AAC Cuts at: 6207 6207 Size: 8617 KpnI G_GTAC'C Cuts at: 5942 5942 Size: 8617 MfeI C'AATT_G Cuts at: 2175 3081 4617 6216 2175 Size: 906 1536 1599 4576 Fragments arranged by size: 4576 1599 1536 906 MluI A'CGCG_T Cuts at: 8493 8493 Size: 8617 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3332 7731 7915 1305 Size: 2027 4399 184 2007 Fragments arranged by size: 4399 2027 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4127 10 Size: 54 1425 343 2295 4500 Fragments arranged by size: 4500 2295 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2925 3101 4926 5018 6575 6934 7093 Size: 648 1759 176 1825 92 1557 359 159 Cuts at: 7093 518 Size: 2042 Fragments arranged by size: 2042 1825 1759 1557 648 359 176 159 92 MspA1I CmG'CkG Cuts at: 1606 2615 2792 3260 4859 6033 6710 7858 8103 Size: 1009 177 468 1599 1174 677 1148 245 Cuts at: 8103 1606 Size: 2120 Fragments arranged by size: 2120 1599 1174 1148 1009 677 468 245 177 NcoI C'CATG_G Cuts at: 513 1065 2004 2845 513 Size: 552 939 841 6285 Fragments arranged by size: 6285 939 841 552 NdeI CA'TA_TG Cuts at: 387 3712 387 Size: 3325 5292 NheI G'CTAG_C Cuts at: 1052 2518 1052 Size: 1466 7151 NotI GC'GGCC_GC Cuts at: 6064 6064 Size: 8617 NsiI A_TGCA'T Cuts at: 5025 5025 Size: 8617 NspI r_CATG'y Cuts at: 1132 1229 3343 5373 5590 5975 7520 1132 Size: 97 2114 2030 217 385 1545 2229 Fragments arranged by size: 2229 2114 2030 1545 385 217 97 PasI CC'CwG_GG Cuts at: 1348 2675 3919 1348 Size: 1327 1244 6046 Fragments arranged by size: 6046 1327 1244 PciI A'CATG_T Cuts at: 5369 5586 7516 5369 Size: 217 1930 6470 Fragments arranged by size: 6470 1930 217 PflMI CCAn_nnn'nTGG Cuts at: 1108 2846 1108 Size: 1738 6879 PfoI T'CCnGG_A Cuts at: 2737 2806 2989 4556 2737 Size: 69 183 1567 6798 Fragments arranged by size: 6798 1567 183 69 PmeI GTTT'AAAC Cuts at: 5921 5921 Size: 8617 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3271 3303 6686 6718 8199 8231 3271 Size: 32 3383 32 1481 32 3657 Fragments arranged by size: 3657 3383 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8617 PsiI TTA'TAA Cuts at: 6187 6187 Size: 8617 PstI C_TGCA'G Cuts at: 830 5969 830 Size: 5139 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8617 PvuII CAG'CTG Cuts at: 2615 3260 4859 2615 Size: 645 1599 6373 Fragments arranged by size: 6373 1599 645 SacI G_AGCT'C Cuts at: 5199 5199 Size: 8617 SalI G'TCGA_C Cuts at: 1936 5959 1936 Size: 4023 4594 SapI GCTCTTCn'nnn_ Cuts at: 3468 3468 Size: 8617 SbfI CC_TGCA'GG Cuts at: 5969 5969 Size: 8617 ScaI AGT'ACT Cuts at: 5388 6865 8577 5388 Size: 1477 1712 5428 Fragments arranged by size: 5428 1712 1477 SexAI A'CCwGG_T Cuts at: 2710 2710 Size: 8617 SfcI C'TryA_G Cuts at: 826 1045 2109 4716 5343 5965 7100 7781 7972 Size: 219 1064 2607 627 622 1135 681 191 Cuts at: 7972 826 Size: 1471 Fragments arranged by size: 2607 1471 1135 1064 681 627 622 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8617 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8617 SmaI CCC'GGG Cuts at: 1872 5944 1872 Size: 4072 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2088 2692 3635 6721 7622 7884 Size: 197 926 145 604 943 3086 901 262 Cuts at: 7884 820 Size: 1553 Fragments arranged by size: 3086 1553 943 926 901 604 262 197 145 SnaBI TAC'GTA Cuts at: 493 6408 493 Size: 5915 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8617 SphI G_CATG'C Cuts at: 1132 5975 1132 Size: 4843 3774 SspI AAT'ATT Cuts at: 5 52 5750 6541 5 Size: 47 5698 791 2081 Fragments arranged by size: 5698 2081 791 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 8617 StyI C'CwwG_G Cuts at: 513 1065 2004 2845 4137 6056 513 Size: 552 939 841 1292 1919 3074 Fragments arranged by size: 3074 1919 1292 939 841 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3032 3308 4916 4940 5787 6644 6661 Size: 1255 1452 276 1608 24 847 857 17 Cuts at: 6661 6814 6999 7444 325 Size: 153 185 445 1498 Fragments arranged by size: 1608 1498 1452 1255 857 847 445 276 185 153 24 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3684 4006 4593 Size: 274 80 33 51 1443 1707 322 587 Cuts at: 4593 5095 5386 5551 6863 8575 96 Size: 502 291 165 1312 1712 138 Fragments arranged by size: 1712 1707 1443 1312 587 502 322 291 274 165 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4102 4134 4387 4419 8577 8609 4102 Size: 32 253 32 4158 32 4110 Fragments arranged by size: 4158 4110 253 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8617 XbaI T'CTAG_A Cuts at: 5953 5953 Size: 8617 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2132 4120 4135 2132 Size: 1988 15 6614 Fragments arranged by size: 6614 1988 15 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8617 XmaI C'CCGG_G Cuts at: 1870 5942 1870 Size: 4072 4545 XmnI GAAnn'nnTTC Cuts at: 3150 6746 3150 Size: 3596 5021 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6425 276 Size: 53 83 186 936 4891 2468 Fragments arranged by size: 4891 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI