(Linear) (Six-base) MAPSORT of: KIEE2433.seq Check: 4346 from: 1 to: 3912 KIEE2433.orf With 182 enzymes: SgfI * February 22, 2013 15:31 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1132 1364 2159 2386 2981 3010 3912 Size: 1132 232 795 227 595 29 902 Fragments arranged by size: 1132 902 795 595 232 227 29 AflII C'TTAA_G Cuts at: 0 687 3912 Size: 687 3225 AflIII A'CryG_T Cuts at: 0 3364 3581 3912 Size: 3364 217 331 Fragments arranged by size: 3364 331 217 AhdI GACnn_n'nnGTC Cuts at: 0 707 3912 Size: 707 3205 AleI CACnn'nnGTG Cuts at: 0 2921 3912 Size: 2921 991 AlwNI CAG_nnn'CTG Cuts at: 0 3759 3912 Size: 3759 153 ApoI r'AATT_y Cuts at: 0 50 125 1573 2326 3800 3912 Size: 50 75 1448 753 1474 112 Fragments arranged by size: 1474 1448 753 112 75 50 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 2495 2528 3912 Size: 2495 33 1384 Fragments arranged by size: 2495 1384 33 BamHI G'GATC_C Cuts at: 0 762 3912 Size: 762 3150 BanI G'GyrC_C Cuts at: 0 625 3912 Size: 625 3287 BanII G_rGCy'C Cuts at: 0 3194 3699 3912 Size: 3194 505 213 Fragments arranged by size: 3194 505 213 BbsI GAAGACnn'nnnn_ Cuts at: 0 427 2893 3912 Size: 427 2466 1019 Fragments arranged by size: 2466 1019 427 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2083 2117 3912 Size: 2083 34 1795 Fragments arranged by size: 2083 1795 34 BciVI GTATCCnnnnn_n' Cuts at: 0 151 1667 2212 3912 Size: 151 1516 545 1700 Fragments arranged by size: 1700 1516 545 151 BclI T'GATC_A Cuts at: 0 612 2652 3912 Size: 612 2040 1260 Fragments arranged by size: 2040 1260 612 BfrBI ATG'CAT Cuts at: 0 3018 3912 Size: 3018 894 BglI GCCn_nnn'nGGC Cuts at: 0 262 845 3912 Size: 262 583 3067 Fragments arranged by size: 3067 583 262 BglII A'GATC_T Cuts at: 0 2792 3042 3912 Size: 2792 250 870 Fragments arranged by size: 2792 870 250 BlpI GC'TnA_GC Cuts at: 0 314 3912 Size: 314 3598 BmtI G_CTAG'C Cuts at: 0 517 3912 Size: 517 3395 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 112 923 1151 3912 Size: 112 811 228 2761 Fragments arranged by size: 2761 811 228 112 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 554 755 1007 1034 1097 1235 1262 3912 Size: 554 201 252 27 63 138 27 2650 Fragments arranged by size: 2650 554 252 201 138 63 27 27 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 68 1651 3912 Size: 68 1583 2261 Fragments arranged by size: 2261 1583 68 BsaI GGTCTCn'nnnn_ Cuts at: 0 658 1833 3533 3912 Size: 658 1175 1700 379 Fragments arranged by size: 1700 1175 658 379 BsaAI yAC'GTr Cuts at: 0 162 3912 Size: 162 3750 BsaBI GATnn'nnATC Cuts at: 0 2311 2651 3912 Size: 2311 340 1261 Fragments arranged by size: 2311 1261 340 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 966 996 1095 1125 1284 1314 3912 Size: 966 30 99 30 159 30 2598 Fragments arranged by size: 2598 966 159 99 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 929 1238 3659 3912 Size: 929 309 2421 253 Fragments arranged by size: 2421 929 309 253 BseYI C'CCAG_C Cuts at: 0 569 3768 3912 Size: 569 3199 144 Fragments arranged by size: 3199 569 144 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2555 3587 3912 Size: 2555 1032 325 Fragments arranged by size: 2555 1032 325 BsiHKAI G_wGCw'C Cuts at: 0 320 864 2988 3194 3912 Size: 320 544 2124 206 718 Fragments arranged by size: 2124 718 544 320 206 BsmI GAATG_Cn' Cuts at: 0 447 1919 2975 3912 Size: 447 1472 1056 937 Fragments arranged by size: 1472 1056 937 447 Bsp1286I G_dGCh'C Cuts at: 0 320 864 2988 3194 3699 3912 Size: 320 544 2124 206 505 213 Fragments arranged by size: 2124 544 505 320 213 206 BspHI T'CATG_A Cuts at: 0 2882 3270 3912 Size: 2882 388 642 Fragments arranged by size: 2882 642 388 BsrDI GCAATG_nn' Cuts at: 0 265 1543 2123 3912 Size: 265 1278 580 1789 Fragments arranged by size: 1789 1278 580 265 BsrGI T'GTAC_A Cuts at: 0 1679 2001 3546 3912 Size: 1679 322 1545 366 Fragments arranged by size: 1679 1545 366 322 BssSI C'ACGA_G Cuts at: 0 1568 1723 3912 Size: 1568 155 2189 Fragments arranged by size: 2189 1568 155 BstAPI GCAn_nnn'nTGC Cuts at: 0 1807 2281 3912 Size: 1807 474 1631 Fragments arranged by size: 1807 1631 474 BstBI TT'CG_AA Cuts at: 0 1272 3912 Size: 1272 2640 BstXI CCAn_nnnn'nTGG Cuts at: 0 1098 1381 2559 3912 Size: 1098 283 1178 1353 Fragments arranged by size: 1353 1178 1098 283 BstYI r'GATC_y Cuts at: 0 762 1718 2792 3042 3912 Size: 762 956 1074 250 870 Fragments arranged by size: 1074 956 870 762 250 BtgI C'CryG_G Cuts at: 0 840 3912 Size: 840 3072 BtsI GCAGTG_nn' Cuts at: 0 3575 3912 Size: 3575 337 DraI TTT'AAA Cuts at: 0 1400 1505 2445 2819 3912 Size: 1400 105 940 374 1093 Fragments arranged by size: 1400 1093 940 374 105 EaeI y'GGCC_r Cuts at: 0 2120 3912 Size: 2120 1792 EarI CTCTTCn'nnn_ Cuts at: 0 1463 2945 3912 Size: 1463 1482 967 Fragments arranged by size: 1482 1463 967 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 384 2194 3912 Size: 384 1810 1718 Fragments arranged by size: 1810 1718 384 EcoICRI GAG'CTC Cuts at: 0 3192 3912 Size: 3192 720 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 554 755 1007 1034 1097 1132 1235 1262 Size: 554 201 252 27 63 35 103 27 Cuts at: 1262 1364 2159 2386 2981 3010 3912 Size: 102 795 227 595 29 902 Fragments arranged by size: 902 795 595 554 252 227 201 103 102 63 35 29 27 27 EcoNI CCTnn'n_nnAGG Cuts at: 0 806 1112 3912 Size: 806 306 2800 Fragments arranged by size: 2800 806 306 EcoO109I rG'GnC_Cy Cuts at: 0 3705 3912 Size: 3705 207 EcoRI G'AATT_C Cuts at: 0 2326 3912 Size: 2326 1586 EcoRV GAT'ATC Cuts at: 0 250 3912 Size: 250 3662 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 196 228 414 446 693 725 1284 1316 Size: 196 32 186 32 247 32 559 32 Cuts at: 1316 1363 1395 1593 1625 2418 2450 3215 3247 Size: 47 32 198 32 793 32 765 32 Cuts at: 3247 3654 3686 3912 Size: 407 32 226 Fragments arranged by size: 793 765 559 407 247 226 198 196 186 47 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 290 3234 3912 Size: 290 2944 678 Fragments arranged by size: 2944 678 290 MfeI C'AATT_G Cuts at: 0 170 1076 2612 3912 Size: 170 906 1536 1300 Fragments arranged by size: 1536 1300 906 170 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1327 3912 Size: 1327 2585 MscI TGG'CCA Cuts at: 0 2122 3912 Size: 2122 1790 MslI CAynn'nnrTG Cuts at: 0 920 1096 2921 3013 3912 Size: 920 176 1825 92 899 Fragments arranged by size: 1825 920 899 176 92 MspA1I CmG'CkG Cuts at: 0 610 787 1255 2854 3912 Size: 610 177 468 1599 1058 Fragments arranged by size: 1599 1058 610 468 177 NcoI C'CATG_G Cuts at: 0 840 3912 Size: 840 3072 NdeI CA'TA_TG Cuts at: 0 1707 3912 Size: 1707 2205 NheI G'CTAG_C Cuts at: 0 513 3912 Size: 513 3399 NsiI A_TGCA'T Cuts at: 0 3020 3912 Size: 3020 892 NspI r_CATG'y Cuts at: 0 1338 3368 3585 3912 Size: 1338 2030 217 327 Fragments arranged by size: 2030 1338 327 217 PasI CC'CwG_GG Cuts at: 0 670 1914 3912 Size: 670 1244 1998 Fragments arranged by size: 1998 1244 670 PciI A'CATG_T Cuts at: 0 3364 3581 3912 Size: 3364 217 331 Fragments arranged by size: 3364 331 217 PflMI CCAn_nnn'nTGG Cuts at: 0 841 3912 Size: 841 3071 PfoI T'CCnGG_A Cuts at: 0 732 801 984 2551 3912 Size: 732 69 183 1567 1361 Fragments arranged by size: 1567 1361 732 183 69 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1266 1298 3912 Size: 1266 32 2614 Fragments arranged by size: 2614 1266 32 PvuII CAG'CTG Cuts at: 0 610 1255 2854 3912 Size: 610 645 1599 1058 Fragments arranged by size: 1599 1058 645 610 SacI G_AGCT'C Cuts at: 0 3194 3912 Size: 3194 718 SapI GCTCTTCn'nnn_ Cuts at: 0 1463 3912 Size: 1463 2449 ScaI AGT'ACT Cuts at: 0 3383 3912 Size: 3383 529 SexAI A'CCwGG_T Cuts at: 0 705 3912 Size: 705 3207 SfcI C'TryA_G Cuts at: 0 104 2711 3338 3912 Size: 104 2607 627 574 Fragments arranged by size: 2607 627 574 104 SmlI C'TyrA_G Cuts at: 0 83 687 1630 3912 Size: 83 604 943 2282 Fragments arranged by size: 2282 943 604 83 SspI AAT'ATT Cuts at: 0 3745 3912 Size: 3745 167 StyI C'CwwG_G Cuts at: 0 840 2132 3912 Size: 840 1292 1780 Fragments arranged by size: 1780 1292 840 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1027 1303 2911 2935 3782 3912 Size: 1027 276 1608 24 847 130 Fragments arranged by size: 1608 1027 847 276 130 24 TatI w'GTAC_w Cuts at: 0 1679 2001 2588 3090 3381 3546 3912 Size: 1679 322 587 502 291 165 366 Fragments arranged by size: 1679 587 502 366 322 291 165 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2097 2129 2382 2414 3912 Size: 2097 32 253 32 1498 Fragments arranged by size: 2097 1498 253 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 127 2115 2130 3912 Size: 127 1988 15 1782 Fragments arranged by size: 1988 1782 127 15 XmnI GAAnn'nnTTC Cuts at: 0 1145 3912 Size: 1145 2767 Enzymes that do cut: AcuI AflII AflIII AhdI AleI AlwNI ApoI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BsrDI BsrGI BssSI BstAPI BstBI BstXI BstYI BtgI BtsI DraI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV Hin4I HincII MfeI MmeI MscI MslI MspA1I NcoI NdeI NheI NsiI NspI PasI PciI PflMI PfoI PpiI PvuII SacI SapI ScaI SexAI SfcI SmlI SspI StyI TaqII TatI TstI XcmI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AgeI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BbeI BbvCI Bme1580I BmgBI BmrI Bpu10I BsaHI BsaWI BsiEI BsiWI BsmBI BspEI BspMI BsrBI BsrFI BssHII BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraIII DrdI EagI FalI FseI FspI FspAI HaeII HindIII HpaI KasI KpnI MluI NaeI NarI NgoMIV NotI NruI PacI PmeI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacII SalI SanDI SbfI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI SwaI Tth111I XbaI XhoI XmaI ZraI