################################## # NO CUT ENZYMES ( 113 enzymes) # ################################## AatII AccI AclI AcyI AflII AgeI Alw44I AlwNI ApaI ApaLI AscI AseI AsnI AspI AviII AvrII BalI BbeI BbvCI BcgI BciVI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaBI BsaHI BsaI BsiWI BsmBI Bsp1407I BspHI BsrGI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I CelII CpoI DraI DraIII DrdI DsaI EcoO65I EcoRI EcoT14I EcoT22I FseI FspI Hin1I HpaI KasI KspI MaeI MamI MfeI MluI MluNI MscI MspA1I MunI NarI NcoI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacII SalI SapI SbfI SexAI SfcI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tth111I Van91I XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### Acc65I 649 GGTACC G^GTACC AccIII 746 TCCGGA T^CCGGA AcsI 864 AAATTT [GA]^AATT[CT] AflIII 876 ACATGT A^C[GA][CT]GT AhdI 961 GACTTTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 112 CCTGAGG CC^T[ACGT]AGG Aor51HI 1316 AGCGCT AGC^GCT ApoI 864 AAATTT [GA]^AATT[CT] Asp718I 649 GGTACC G^GTACC AspEI 961 GACTTTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 123 GAGCTC G[AT]GC[AT]^C BamHI 1544 GGATCC G^GATCC BanI 649 GGTACC G^G[CT][GA]CC BbrPI 1549 CACGTG CAC^GTG BclI 1622 TGATCA T^GATCA BseAI 746 TCCGGA T^CCGGA BsgI 988 CTGCAC GTGCAG(16/14) BsiHKAI 123 GAGCTC G[AT]GC[AT]^C BsmI 981 GAATGC GAATGC(1/-1) BspDI 544 ATCGAT AT^CGAT BspEI 746 TCCGGA T^CCGGA BspLU11I 876 ACATGT A^CATGT BspMI 659 ACCTGC ACCTGC(4/8) BsrDI 1497 CATTGC GCAATG(2/0) BsrI 471 CCAGT ACTGG(1/-1) BstXI 822 CCAGCGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 112 CCTGAGG CC^T[ACGT]AGG ClaI 544 ATCGAT AT^CGAT DraII 182 AGGCCCC [GA]G^G[ACGT]CC[CT] EagI 6 CGGCCG C^GGCCG Eam1105I 961 GACTTTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 6 CGGCCG C^GGCCG Eco47III 1316 AGCGCT AGC^GCT Eco52I 6 CGGCCG C^GGCCG Eco81I 112 CCTGAGG CC^T[ACGT]AGG EcoNI 1197 CCTCTACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 182 AGGCCCC [GA]G^G[ACGT]CC[CT] FbaI 1622 TGATCA T^GATCA HaeII 1316 AGCGCT [GA]GCGC^[CT] HincII 958 GTTGAC GT[CT]^[GA]AC HindII 958 GTTGAC GT[CT]^[GA]AC HindIII 1075 AAGCTT A^AGCTT KpnI 649 GGTACC GGTAC^C MroI 746 TCCGGA T^CCGGA MseI 332 TTAA T^TAA NaeI 165 GCCGGC GCC^GGC NdeI 641 CATATG CA^TATG NgoMIV 165 GCCGGC G^CCGGC NspI 876 ACATGT [GA]CATG^[CT] PmaCI 1549 CACGTG CAC^GTG PmlI 1549 CACGTG CAC^GTG SacI 123 GAGCTC GAGCT^C ScaI 422 AGTACT AGT^ACT SmlI 508 CTCAAG C^T[CT][GA]AG Tru9I 332 TTAA T^TAA ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### Asp700I 1314 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1377 GAACAACTTC AvaI 128 CCCGGG C^[CT]CG[GA]G 635 CCCGGG BanII 123 GAGCTC G[GA]GC[CT]^C 1533 GGGCTC BpmI 794 CTCCAG CTGGAG(16/14) 1594 CTCCAG BsaAI 567 CACGTA [CT]AC^GT[GA] 1549 CACGTG BseRI 740 CTCCTC GAGGAG(10/8) 300 GAGGAG BsiEI 6 CGGCCG CG[GA][CT]^CG 75 CGGTCG BsoBI 128 CCCGGG C^[CT]CG[GA]G 635 CCCGGG BsrBI 103 GAGCGG CCGCTC(-3/-3) 253 GAGCGG BsrFI 8 GCCGGT [GA]^CCGG[CT] 165 GCCGGC BssHII 173 GCGCGC G^CGCGC 175 GCGCGC Cfr10I 8 GCCGGT [GA]^CCGG[CT] 165 GCCGGC EaeI 6 CGGCCG [CT]^GGCC[GA] 276 TGGCCG EarI 1243 CTCTTC CTCTTC(1/4) 1656 GAAGAG EcoRV 562 GATATC GAT^ATC 802 GATATC HgaI 1173 GACGC GACGC(5/10) 80 GCGTC Ksp632I 1243 CTCTTC CTCTTC(1/4) 1656 GAAGAG MaeIII 1033 GTGAC ^GT[ACGT]AC 1600 GTGAC SfaNI 894 GATGC GCATC(5/9) 1271 GCATC SmaI 128 CCCGGG CCC^GGG 635 CCCGGG Tsp45I 1033 GTGAC ^GT[GC]AC 1600 GTGAC XmaCI 128 CCCGGG C^CCGGG 635 CCCGGG XmaI 128 CCCGGG C^CCGGG 635 CCCGGG XmnI 1314 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1377 GAACAACTTC ######################################### # 3 OR MORE CUT ENZYMES ( 66 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AvaII BbsI BbvI BcnI BmyI BpuAI BsaJI BsaWI BsiYI BslI BsmAI BsmFI Bsp1286I BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeII MboI MboII MflI MnlI MslI MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI TaqI TfiI TseI Tsp509I TspRI TthHB8I XhoII