Restriction map for pFN21AE5640
 (Circular) (Six-base) MAPSORT of: pFN21AE5640.seq  Check: 6594  from: 1  to: 6400



pFN21AE5640  6400 bp

 With 182 enzymes: SgfI * 

                        December 8, 2014 13:41  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    4210     278 
   Size:         53      83     186     936    2674    2468

  Fragments arranged by size: 

                2674    2468     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    3743    1937 
   Size:       1806    4594
 
Acc65I G'GTAC_C

Cuts at:   2654    3721    2654 
   Size:       1067    5333
 
AclI AA'CG_TT

Cuts at:   1563    4527    4900    1563 
   Size:       2964     373    3063

  Fragments arranged by size: 

                3063    2964     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2708    2888    3338    3480    4463    5847    6115    1632 
   Size:       1076     180     450     142     983    1384     268    1917

  Fragments arranged by size: 

                1917    1384    1076     983     450     268     180     142
            
 
AfeI AGC'GCT

Cuts at:   3323    3323 
   Size:       6400
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    6203
 
AflIII A'CryG_T

Cuts at:   1203    2881    5299    6276    1203 
   Size:       1678    2418     977    1327

  Fragments arranged by size: 

                2418    1678    1327     977
 
AgeI A'CCGG_T

Cuts at:   5231    5231 
   Size:       6400
 
AhdI GACnn_n'nnGTC

Cuts at:   2971    5129    2971 
   Size:       2158    4242
 
AleI CACnn'nnGTG

Cuts at:   1166    2576    1166 
   Size:       1410    4990
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    2162    2625    1184 
   Size:        978     463    4959

  Fragments arranged by size: 

                4959     978     463
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2798    2830    2798 
   Size:         32    6368
 
AlwNI CAG_nnn'CTG

Cuts at:   4143    5715    4143 
   Size:       1572    4828
 
ApaLI G'TGCA_C

Cuts at:   4457    5613    4457 
   Size:       1156    5244
 
ApoI r'AATT_y

Cuts at:   1493    2869    3709    3935    4087    6196    1493 
   Size:       1376     840     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1376     840     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    4954     160 
   Size:        872    3922    1606

  Fragments arranged by size: 

                3922    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6400
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2133    2640    3725    1870 
   Size:         73     190     507    1085    4545

  Fragments arranged by size: 

                4545    1085     507     190      73
 
BamHI G'GATC_C

Cuts at:   3549    3730    4097    3549 
   Size:        181     367    5852

  Fragments arranged by size: 

                5852     367     181
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2654    3721    5176     618 
   Size:        325     217     627     867    1067    1455    1842

  Fragments arranged by size: 

                1842    1455    1067     867     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2132    3542    3719    1391 
   Size:        741    1410     177    4072

  Fragments arranged by size: 

                4072    1410     741     177
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2778    2936    3035     928 
   Size:        971     879     158      99    4293

  Fragments arranged by size: 

                4293     971     879     158      99
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    4591    4625    1903 
   Size:         34    2654      34    3678

  Fragments arranged by size: 

                3678    2654      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   4292    5502    4292 
   Size:       1210    5190
 
BclI T'GATC_A

Cuts at:   1548    3627    1548 
   Size:       2079    4321
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    5011     136 
   Size:        107     122      71     631     605    3339    1525

  Fragments arranged by size: 

                3339    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   6395    6395 
   Size:       6400
 
BlpI GC'TnA_GC

Cuts at:   3817    3817 
   Size:       6400
 
Bme1580I G_kGCm'C

Cuts at:   1790    3014    4461    5617    1790 
   Size:       1224    1447    1156    2573

  Fragments arranged by size: 

                2573    1447    1224    1156
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       6400
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    4131    5089     442 
   Size:        416      53      83     399      24    2714     958    1753

  Fragments arranged by size: 

                2714    1753     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       6400
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2784    3584    5060    1239 
   Size:       1545     800    1476    2579

  Fragments arranged by size: 

                2579    1545    1476     800
 
Bpu10I CC'TnA_GC

Cuts at:   2256    2256 
   Size:       6400
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2498    4525    5390    5688    2498 
   Size:       2027     865     298    3210

  Fragments arranged by size: 

                3210    2027     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    5063     882 
   Size:        617    3564    2219

  Fragments arranged by size: 

                3564    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    2574    3556    4191     493 
   Size:        711    1370     982     635    2702

  Fragments arranged by size: 

                2702    1370     982     711     635
 
BsaBI GATnn'nnATC

Cuts at:   6394    6394 
   Size:       6400
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    4207    4589    5224     275 
   Size:         53      83     186     936    2674     382     635    1451

  Fragments arranged by size: 

                2674    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   2078    2301    2751    3546    4833    5231    5505    5652    6063 
   Size:        223     450     795    1287     398     274     147     411

Cuts at:   6063    2078 
   Size:       2415

  Fragments arranged by size: 

                2415    1287     795     450     411     398     274     223
                 147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2946    2976    3582    3612    1178 
   Size:         30     165      30    1543      30     606      30    3966

  Fragments arranged by size: 

                3966    1543     606     165      30      30      30      30
            
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2320    2736    1186 
   Size:       1134     416    4850

  Fragments arranged by size: 

                4850    1134     416
 
BseYI C'CCAG_C

Cuts at:   5603    6099    5603 
   Size:        496    5904
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2978    1163 
   Size:        573    1242    4585

  Fragments arranged by size: 

                4585    1242     573
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2014    2083    3850    4611    5639    1540 
   Size:        275     186      13      69    1767     761    1028    2301

  Fragments arranged by size: 

                2301    1767    1028     761     275     186      69      13
            
 
BsiHKAI G_wGCw'C

Cuts at:   2132    4461    4546    5617    2132 
   Size:       2329      85    1071    2915

  Fragments arranged by size: 

                2915    2329    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2992    3911    4004    2992 
   Size:        919      93    5388

  Fragments arranged by size: 

                5388     919      93
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2132    3014    3542    3719    4461    4546    5617 
   Size:        399     342     882     528     177     742      85    1071

Cuts at:   5617    1391 
   Size:       2174

  Fragments arranged by size: 

                2174    1071     882     742     528     399     342     177
                  85
 
BspEI T'CCGG_A

Cuts at:   2751    6063    2751 
   Size:       3312    3088
 
BspHI T'CATG_A

Cuts at:   4289    6019    4289 
   Size:       1730    4670
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2673    3755     844 
   Size:       1829    1082    3489

  Fragments arranged by size: 

                3489    1829    1082
 
BsrBI CCG'CTC

Cuts at:   1394    1823    2110    2260    4287    1394 
   Size:        429     287     150    2027    3507

  Fragments arranged by size: 

                3507    2027     429     287     150
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    3501    4895    5069      65 
   Size:       1183    2253    1394     174    1396

  Fragments arranged by size: 

                2253    1396    1394    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    2013    2170    5044    5231    1700 
   Size:        110     203     157    2874     187    2869

  Fragments arranged by size: 

                2874    2869     203     187     157     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       6400
 
BssHII G'CGCG_C

Cuts at:   1925    2178    2180    1925 
   Size:        253       2    6145

  Fragments arranged by size: 

                6145     253       2
 
BssSI C'ACGA_G

Cuts at:   4460    5472    4460 
   Size:       1012    5388
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       6400
 
BstBI TT'CG_AA

Cuts at:   5204    5204 
   Size:       6400
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       6400
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2834    2834 
   Size:       6400
 
BstYI r'GATC_y

Cuts at:   1972    3066    3439    3549    3730    4097    4482    4499    5951 
   Size:       1094     373     110     181     367     385      17    1452

Cuts at:   5951    6037    6049    6395    1972 
   Size:         86      12     346    1977

  Fragments arranged by size: 

                1977    1452    1094     385     373     367     346     181
                 110      86      17      12
 
Bsu36I CC'TnA_GG

Cuts at:   2118    2118 
   Size:       6400
 
BtgI C'CryG_G

Cuts at:    513    1065     513 
   Size:        552    5848
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    5661

  Fragments arranged by size: 

                5661     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2314    3916    4710    4730    2314 
   Size:       1602     794      20    3984

  Fragments arranged by size: 

                3984    1602     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    2550    1554 
   Size:        996    5404
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    6365
 
DraI TTT'AAA

Cuts at:   3704    3704 
   Size:       6400
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    5407     809 
   Size:        507    4091    1802

  Fragments arranged by size: 

                4091    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2011    2281    3847 
   Size:         54    1425      50     275      18     181     270    1566

Cuts at:   3847    4736    6365       8 
   Size:        889    1629      43

  Fragments arranged by size: 

                1629    1566    1425     889     275     270     181      54
                  50      43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2011    3847    1537 
   Size:        275     199    1836    4090

  Fragments arranged by size: 

                4090    1836     275     199
 
EarI CTCTTCn'nnn_

Cuts at:   1353    3254    3656    4330    1353 
   Size:       1901     402     674    3423

  Fragments arranged by size: 

                3423    1901     674     402
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4987    5361    5507     387 
   Size:       1064     138    3398     374     146    1280

  Fragments arranged by size: 

                3398    1280    1064     374     146     138
 
EcoICRI GAG'CTC

Cuts at:   2130    2130 
   Size:       6400
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2708    2784    2888    3338    3480    3584    4463 
   Size:        393    1076      76     104     450     142     104     879

Cuts at:   4463    5060    5847    6115    1239 
   Size:        597     787     268    1524

  Fragments arranged by size: 

                1524    1076     879     787     597     450     393     268
                 142     104     104      76
 
EcoNI CCTnn'n_nnAGG

Cuts at:   3206    3206 
   Size:       6400
 
EcoO109I rG'GnC_Cy

Cuts at:   2188    2188 
   Size:       6400
 
EcoRI G'AATT_C

Cuts at:   3709    4087    3709 
   Size:        378    6022
 
EcoRV GAT'ATC

Cuts at:   2569    2809    2569 
   Size:        240    6160
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   3328    3403    3328 
   Size:         75    6325
 
FspI TGC'GCA

Cuts at:   4906    4906 
   Size:       6400
 
HaeII r_GCGC'y

Cuts at:   1678    3325    5547    1678 
   Size:       1647    2222    2531

  Fragments arranged by size: 

                2531    2222    1647
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2048    2080    2231    2263    2957 
   Size:         32     135      32     476      32     151      32     694

Cuts at:   2957    2989    5041    5073    5115    5147    1373 
   Size:         32    2052      32      42      32    2626

  Fragments arranged by size: 

                2626    2052     694     476     151     135      42      32
                  32      32      32      32      32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2965    3744    3990     669 
   Size:        988     281    1027     779     246    3079

  Fragments arranged by size: 

                3079    1027     988     779     281     246
 
HindIII A'AGCT_T

Cuts at:    748    3080     748 
   Size:       2332    4068
 
HpaI GTT'AAC

Cuts at:   3990    3990 
   Size:       6400
 
KpnI G_GTAC'C

Cuts at:   2658    3725    2658 
   Size:       1067    5333
 
MfeI C'AATT_G

Cuts at:   3999    3999 
   Size:       6400
 
MluI A'CGCG_T

Cuts at:   6276    6276 
   Size:       6400
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    3431    5514    5698    1305 
   Size:       2126    2083     184    2007

  Fragments arranged by size: 

                2126    2083    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    4578

  Fragments arranged by size: 

                4578    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2397    2576    3167    4358    4717    4876     518 
   Size:        648    1231     179     591    1191     359     159    2042

  Fragments arranged by size: 

                2042    1231    1191     648     591     359     179     159
            
 
MspA1I CmG'CkG

Cuts at:   1606    3816    4493    5641    5886    1606 
   Size:       2210     677    1148     245    2120

  Fragments arranged by size: 

                2210    2120    1148     677     245
 
NaeI GCC'GGC

Cuts at:   2172    2172 
   Size:       6400
 
NcoI C'CATG_G

Cuts at:    513    1065     513 
   Size:        552    5848
 
NdeI CA'TA_TG

Cuts at:    387    2647     387 
   Size:       2260    4140
 
NgoMIV G'CCGG_C

Cuts at:   2170    2170 
   Size:       6400
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       6400
 
NotI GC'GGCC_GC

Cuts at:   3847    3847 
   Size:       6400
 
NspI r_CATG'y

Cuts at:   1132    1229    2885    3758    5303    1132 
   Size:         97    1656     873    1545    2229

  Fragments arranged by size: 

                2229    1656    1545     873      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       6400
 
PciI A'CATG_T

Cuts at:   2881    5299    2881 
   Size:       2418    3982
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       6400
 
PmeI GTTT'AAAC

Cuts at:   3704    3704 
   Size:       6400
 
PmlI CAC'GTG

Cuts at:   3556    3556 
   Size:       6400
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4469    4501    5982    6014    4469 
   Size:         32    1481      32    4855

  Fragments arranged by size: 

                4855    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       6400
 
PsiI TTA'TAA

Cuts at:   2781    3970    2781 
   Size:       1189    5211
 
PstI C_TGCA'G

Cuts at:    830    3752     830 
   Size:       2922    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       6400
 
SacI G_AGCT'C

Cuts at:   2132    2132 
   Size:       6400
 
SalI G'TCGA_C

Cuts at:   1936    3742    1936 
   Size:       1806    4594
 
SbfI CC_TGCA'GG

Cuts at:   3752    3752 
   Size:       6400
 
ScaI AGT'ACT

Cuts at:   2429    4648    6360    2429 
   Size:       2219    1712    2469

  Fragments arranged by size: 

                2469    2219    1712
 
SfcI C'TryA_G

Cuts at:    826    1045    3748    4883    5564    5755     826 
   Size:        219    2703    1135     681     191    1471

  Fragments arranged by size: 

                2703    1471    1135     681     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6400
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       6400
 
SmaI CCC'GGG

Cuts at:   1872    2135    2642    3727    1872 
   Size:        263     507    1085    4545

  Fragments arranged by size: 

                4545    1085     507     263
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2513    4504    5405    5667     820 
   Size:        197     926     570    1991     901     262    1553

  Fragments arranged by size: 

                1991    1553     926     901     570     262     197
 
SnaBI TAC'GTA

Cuts at:    493    4191     493 
   Size:       3698    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       6400
 
SphI G_CATG'C

Cuts at:   1132    3758    1132 
   Size:       2626    3774
 
SspI AAT'ATT

Cuts at:      5      52    4324       5 
   Size:         47    4272    2081

  Fragments arranged by size: 

                4272    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       6400
 
StyI C'CwwG_G

Cuts at:    513    1065    3839     513 
   Size:        552    2774    3074

  Fragments arranged by size: 

                3074    2774     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    4427    4444    4597    4782    5227     325 
   Size:       1255    2847      17     153     185     445    1498

  Fragments arranged by size: 

                2847    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2427    2914    3466 
   Size:        274      80      33      51    1443     450     487     552

Cuts at:   3466    3475    4646    6358      96 
   Size:          9    1171    1712     138

  Fragments arranged by size: 

                1712    1443    1171     552     487     450     274     138
                  80      51      33       9
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   6360    6392    6360 
   Size:         32    6368
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       6400
 
XbaI T'CTAG_A

Cuts at:   3736    3736 
   Size:       6400
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       6400
 
XmaI C'CCGG_G

Cuts at:   1870    2133    2640    3725    1870 
   Size:        263     507    1085    4545

  Fragments arranged by size: 

                4545    1085     507     263
 
XmnI GAAnn'nnTTC

Cuts at:   3323    3386    4529    3323 
   Size:         63    1143    5194

  Fragments arranged by size: 

                5194    1143      63
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    4208     276 
   Size:         53      83     186     936    2674    2468

  Fragments arranged by size: 

                2674    2468     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     ApoI
     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbsI     BcgI
    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FspI    HaeII    Hin4I   HincII  HindIII     HpaI
     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NcoI     NdeI   NgoMIV     NheI     NotI     NspI     PasI     PciI
    PflMI     PmeI     PmlI     PpiI    PshAI     PsiI     PstI     PvuI
     SacI     SalI     SbfI     ScaI     SfcI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SspI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     ApaI     AscI    AvrII     BaeI     BbeI    BbvCI    BfrBI
     BplI    BsiWI    BsmBI  BstZ17I   DraIII     FseI    FspAI     KasI
     NarI     NruI     NsiI     PacI     PfoI    PpuMI   PspOMI    PspXI
     PsrI    PvuII    RsrII    SacII    SanDI     SapI    SexAI     SfiI
     SfoI     SrfI     SwaI     XcmI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com