(Circular) (Six-base) MAPSORT of: pFN21AE5640.seq Check: 6594 from: 1 to: 6400 pFN21AE5640 6400 bp With 182 enzymes: SgfI * December 8, 2014 13:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4210 278 Size: 53 83 186 936 2674 2468 Fragments arranged by size: 2674 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3743 1937 Size: 1806 4594 Acc65I G'GTAC_C Cuts at: 2654 3721 2654 Size: 1067 5333 AclI AA'CG_TT Cuts at: 1563 4527 4900 1563 Size: 2964 373 3063 Fragments arranged by size: 3063 2964 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2708 2888 3338 3480 4463 5847 6115 1632 Size: 1076 180 450 142 983 1384 268 1917 Fragments arranged by size: 1917 1384 1076 983 450 268 180 142 AfeI AGC'GCT Cuts at: 3323 3323 Size: 6400 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6203 AflIII A'CryG_T Cuts at: 1203 2881 5299 6276 1203 Size: 1678 2418 977 1327 Fragments arranged by size: 2418 1678 1327 977 AgeI A'CCGG_T Cuts at: 5231 5231 Size: 6400 AhdI GACnn_n'nnGTC Cuts at: 2971 5129 2971 Size: 2158 4242 AleI CACnn'nnGTG Cuts at: 1166 2576 1166 Size: 1410 4990 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2162 2625 1184 Size: 978 463 4959 Fragments arranged by size: 4959 978 463 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2798 2830 2798 Size: 32 6368 AlwNI CAG_nnn'CTG Cuts at: 4143 5715 4143 Size: 1572 4828 ApaLI G'TGCA_C Cuts at: 4457 5613 4457 Size: 1156 5244 ApoI r'AATT_y Cuts at: 1493 2869 3709 3935 4087 6196 1493 Size: 1376 840 226 152 2109 1697 Fragments arranged by size: 2109 1697 1376 840 226 152 AseI AT'TA_AT Cuts at: 160 1032 4954 160 Size: 872 3922 1606 Fragments arranged by size: 3922 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6400 AvaI C'yCGr_G Cuts at: 1870 1943 2133 2640 3725 1870 Size: 73 190 507 1085 4545 Fragments arranged by size: 4545 1085 507 190 73 BamHI G'GATC_C Cuts at: 3549 3730 4097 3549 Size: 181 367 5852 Fragments arranged by size: 5852 367 181 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2654 3721 5176 618 Size: 325 217 627 867 1067 1455 1842 Fragments arranged by size: 1842 1455 1067 867 627 325 217 BanII G_rGCy'C Cuts at: 1391 2132 3542 3719 1391 Size: 741 1410 177 4072 Fragments arranged by size: 4072 1410 741 177 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2778 2936 3035 928 Size: 971 879 158 99 4293 Fragments arranged by size: 4293 971 879 158 99 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4591 4625 1903 Size: 34 2654 34 3678 Fragments arranged by size: 3678 2654 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4292 5502 4292 Size: 1210 5190 BclI T'GATC_A Cuts at: 1548 3627 1548 Size: 2079 4321 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5011 136 Size: 107 122 71 631 605 3339 1525 Fragments arranged by size: 3339 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6395 6395 Size: 6400 BlpI GC'TnA_GC Cuts at: 3817 3817 Size: 6400 Bme1580I G_kGCm'C Cuts at: 1790 3014 4461 5617 1790 Size: 1224 1447 1156 2573 Fragments arranged by size: 2573 1447 1224 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6400 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4131 5089 442 Size: 416 53 83 399 24 2714 958 1753 Fragments arranged by size: 2714 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6400 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2784 3584 5060 1239 Size: 1545 800 1476 2579 Fragments arranged by size: 2579 1545 1476 800 Bpu10I CC'TnA_GC Cuts at: 2256 2256 Size: 6400 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2498 4525 5390 5688 2498 Size: 2027 865 298 3210 Fragments arranged by size: 3210 2027 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5063 882 Size: 617 3564 2219 Fragments arranged by size: 3564 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2574 3556 4191 493 Size: 711 1370 982 635 2702 Fragments arranged by size: 2702 1370 982 711 635 BsaBI GATnn'nnATC Cuts at: 6394 6394 Size: 6400 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4207 4589 5224 275 Size: 53 83 186 936 2674 382 635 1451 Fragments arranged by size: 2674 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2078 2301 2751 3546 4833 5231 5505 5652 6063 Size: 223 450 795 1287 398 274 147 411 Cuts at: 6063 2078 Size: 2415 Fragments arranged by size: 2415 1287 795 450 411 398 274 223 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2946 2976 3582 3612 1178 Size: 30 165 30 1543 30 606 30 3966 Fragments arranged by size: 3966 1543 606 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2320 2736 1186 Size: 1134 416 4850 Fragments arranged by size: 4850 1134 416 BseYI C'CCAG_C Cuts at: 5603 6099 5603 Size: 496 5904 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2978 1163 Size: 573 1242 4585 Fragments arranged by size: 4585 1242 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2014 2083 3850 4611 5639 1540 Size: 275 186 13 69 1767 761 1028 2301 Fragments arranged by size: 2301 1767 1028 761 275 186 69 13 BsiHKAI G_wGCw'C Cuts at: 2132 4461 4546 5617 2132 Size: 2329 85 1071 2915 Fragments arranged by size: 2915 2329 1071 85 BsmI GAATG_Cn' Cuts at: 2992 3911 4004 2992 Size: 919 93 5388 Fragments arranged by size: 5388 919 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2132 3014 3542 3719 4461 4546 5617 Size: 399 342 882 528 177 742 85 1071 Cuts at: 5617 1391 Size: 2174 Fragments arranged by size: 2174 1071 882 742 528 399 342 177 85 BspEI T'CCGG_A Cuts at: 2751 6063 2751 Size: 3312 3088 BspHI T'CATG_A Cuts at: 4289 6019 4289 Size: 1730 4670 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2673 3755 844 Size: 1829 1082 3489 Fragments arranged by size: 3489 1829 1082 BsrBI CCG'CTC Cuts at: 1394 1823 2110 2260 4287 1394 Size: 429 287 150 2027 3507 Fragments arranged by size: 3507 2027 429 287 150 BsrDI GCAATG_nn' Cuts at: 65 1248 3501 4895 5069 65 Size: 1183 2253 1394 174 1396 Fragments arranged by size: 2253 1396 1394 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2013 2170 5044 5231 1700 Size: 110 203 157 2874 187 2869 Fragments arranged by size: 2874 2869 203 187 157 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6400 BssHII G'CGCG_C Cuts at: 1925 2178 2180 1925 Size: 253 2 6145 Fragments arranged by size: 6145 253 2 BssSI C'ACGA_G Cuts at: 4460 5472 4460 Size: 1012 5388 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6400 BstBI TT'CG_AA Cuts at: 5204 5204 Size: 6400 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6400 BstXI CCAn_nnnn'nTGG Cuts at: 2834 2834 Size: 6400 BstYI r'GATC_y Cuts at: 1972 3066 3439 3549 3730 4097 4482 4499 5951 Size: 1094 373 110 181 367 385 17 1452 Cuts at: 5951 6037 6049 6395 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1094 385 373 367 346 181 110 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2118 2118 Size: 6400 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5848 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5661 Fragments arranged by size: 5661 683 56 BtsI GCAGTG_nn' Cuts at: 2314 3916 4710 4730 2314 Size: 1602 794 20 3984 Fragments arranged by size: 3984 1602 794 20 ClaI AT'CG_AT Cuts at: 1554 2550 1554 Size: 996 5404 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6365 DraI TTT'AAA Cuts at: 3704 3704 Size: 6400 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5407 809 Size: 507 4091 1802 Fragments arranged by size: 4091 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2011 2281 3847 Size: 54 1425 50 275 18 181 270 1566 Cuts at: 3847 4736 6365 8 Size: 889 1629 43 Fragments arranged by size: 1629 1566 1425 889 275 270 181 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2011 3847 1537 Size: 275 199 1836 4090 Fragments arranged by size: 4090 1836 275 199 EarI CTCTTCn'nnn_ Cuts at: 1353 3254 3656 4330 1353 Size: 1901 402 674 3423 Fragments arranged by size: 3423 1901 674 402 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4987 5361 5507 387 Size: 1064 138 3398 374 146 1280 Fragments arranged by size: 3398 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2130 2130 Size: 6400 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2708 2784 2888 3338 3480 3584 4463 Size: 393 1076 76 104 450 142 104 879 Cuts at: 4463 5060 5847 6115 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1076 879 787 597 450 393 268 142 104 104 76 EcoNI CCTnn'n_nnAGG Cuts at: 3206 3206 Size: 6400 EcoO109I rG'GnC_Cy Cuts at: 2188 2188 Size: 6400 EcoRI G'AATT_C Cuts at: 3709 4087 3709 Size: 378 6022 EcoRV GAT'ATC Cuts at: 2569 2809 2569 Size: 240 6160 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3328 3403 3328 Size: 75 6325 FspI TGC'GCA Cuts at: 4906 4906 Size: 6400 HaeII r_GCGC'y Cuts at: 1678 3325 5547 1678 Size: 1647 2222 2531 Fragments arranged by size: 2531 2222 1647 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2048 2080 2231 2263 2957 Size: 32 135 32 476 32 151 32 694 Cuts at: 2957 2989 5041 5073 5115 5147 1373 Size: 32 2052 32 42 32 2626 Fragments arranged by size: 2626 2052 694 476 151 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2965 3744 3990 669 Size: 988 281 1027 779 246 3079 Fragments arranged by size: 3079 1027 988 779 281 246 HindIII A'AGCT_T Cuts at: 748 3080 748 Size: 2332 4068 HpaI GTT'AAC Cuts at: 3990 3990 Size: 6400 KpnI G_GTAC'C Cuts at: 2658 3725 2658 Size: 1067 5333 MfeI C'AATT_G Cuts at: 3999 3999 Size: 6400 MluI A'CGCG_T Cuts at: 6276 6276 Size: 6400 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3431 5514 5698 1305 Size: 2126 2083 184 2007 Fragments arranged by size: 2126 2083 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4578 Fragments arranged by size: 4578 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2397 2576 3167 4358 4717 4876 518 Size: 648 1231 179 591 1191 359 159 2042 Fragments arranged by size: 2042 1231 1191 648 591 359 179 159 MspA1I CmG'CkG Cuts at: 1606 3816 4493 5641 5886 1606 Size: 2210 677 1148 245 2120 Fragments arranged by size: 2210 2120 1148 677 245 NaeI GCC'GGC Cuts at: 2172 2172 Size: 6400 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5848 NdeI CA'TA_TG Cuts at: 387 2647 387 Size: 2260 4140 NgoMIV G'CCGG_C Cuts at: 2170 2170 Size: 6400 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6400 NotI GC'GGCC_GC Cuts at: 3847 3847 Size: 6400 NspI r_CATG'y Cuts at: 1132 1229 2885 3758 5303 1132 Size: 97 1656 873 1545 2229 Fragments arranged by size: 2229 1656 1545 873 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6400 PciI A'CATG_T Cuts at: 2881 5299 2881 Size: 2418 3982 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6400 PmeI GTTT'AAAC Cuts at: 3704 3704 Size: 6400 PmlI CAC'GTG Cuts at: 3556 3556 Size: 6400 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4469 4501 5982 6014 4469 Size: 32 1481 32 4855 Fragments arranged by size: 4855 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6400 PsiI TTA'TAA Cuts at: 2781 3970 2781 Size: 1189 5211 PstI C_TGCA'G Cuts at: 830 3752 830 Size: 2922 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6400 SacI G_AGCT'C Cuts at: 2132 2132 Size: 6400 SalI G'TCGA_C Cuts at: 1936 3742 1936 Size: 1806 4594 SbfI CC_TGCA'GG Cuts at: 3752 3752 Size: 6400 ScaI AGT'ACT Cuts at: 2429 4648 6360 2429 Size: 2219 1712 2469 Fragments arranged by size: 2469 2219 1712 SfcI C'TryA_G Cuts at: 826 1045 3748 4883 5564 5755 826 Size: 219 2703 1135 681 191 1471 Fragments arranged by size: 2703 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6400 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6400 SmaI CCC'GGG Cuts at: 1872 2135 2642 3727 1872 Size: 263 507 1085 4545 Fragments arranged by size: 4545 1085 507 263 SmlI C'TyrA_G Cuts at: 820 1017 1943 2513 4504 5405 5667 820 Size: 197 926 570 1991 901 262 1553 Fragments arranged by size: 1991 1553 926 901 570 262 197 SnaBI TAC'GTA Cuts at: 493 4191 493 Size: 3698 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6400 SphI G_CATG'C Cuts at: 1132 3758 1132 Size: 2626 3774 SspI AAT'ATT Cuts at: 5 52 4324 5 Size: 47 4272 2081 Fragments arranged by size: 4272 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6400 StyI C'CwwG_G Cuts at: 513 1065 3839 513 Size: 552 2774 3074 Fragments arranged by size: 3074 2774 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4427 4444 4597 4782 5227 325 Size: 1255 2847 17 153 185 445 1498 Fragments arranged by size: 2847 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2427 2914 3466 Size: 274 80 33 51 1443 450 487 552 Cuts at: 3466 3475 4646 6358 96 Size: 9 1171 1712 138 Fragments arranged by size: 1712 1443 1171 552 487 450 274 138 80 51 33 9 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6360 6392 6360 Size: 32 6368 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6400 XbaI T'CTAG_A Cuts at: 3736 3736 Size: 6400 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6400 XmaI C'CCGG_G Cuts at: 1870 2133 2640 3725 1870 Size: 263 507 1085 4545 Fragments arranged by size: 4545 1085 507 263 XmnI GAAnn'nnTTC Cuts at: 3323 3386 4529 3323 Size: 63 1143 5194 Fragments arranged by size: 5194 1143 63 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4208 276 Size: 53 83 186 936 2674 2468 Fragments arranged by size: 2674 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I DraIII FseI FspAI KasI NarI NruI NsiI PacI PfoI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI