(Linear) (Six-base) MAPSORT of: KIEE5640.seq Check: 6546 from: 1 to: 1695 KIEE5640.orf With 182 enzymes: SgfI * December 5, 2014 13:04 .. Acc65I G'GTAC_C Cuts at: 0 649 1695 Size: 649 1046 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 703 883 1333 1475 1695 Size: 703 180 450 142 220 Fragments arranged by size: 703 450 220 180 142 AfeI AGC'GCT Cuts at: 0 1318 1695 Size: 1318 377 AflIII A'CryG_T Cuts at: 0 876 1695 Size: 876 819 AhdI GACnn_n'nnGTC Cuts at: 0 966 1695 Size: 966 729 AleI CACnn'nnGTG Cuts at: 0 571 1695 Size: 571 1124 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 157 620 1695 Size: 157 463 1075 Fragments arranged by size: 1075 463 157 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 793 825 1695 Size: 793 32 870 Fragments arranged by size: 870 793 32 ApoI r'AATT_y Cuts at: 0 864 1695 Size: 864 831 AvaI C'yCGr_G Cuts at: 0 128 635 1695 Size: 128 507 1060 Fragments arranged by size: 1060 507 128 BamHI G'GATC_C Cuts at: 0 1544 1695 Size: 1544 151 BanI G'GyrC_C Cuts at: 0 649 1695 Size: 649 1046 BanII G_rGCy'C Cuts at: 0 127 1537 1695 Size: 127 1410 158 Fragments arranged by size: 1410 158 127 BbsI GAAGACnn'nnnn_ Cuts at: 0 773 931 1030 1695 Size: 773 158 99 665 Fragments arranged by size: 773 665 158 99 BclI T'GATC_A Cuts at: 0 1622 1695 Size: 1622 73 Bme1580I G_kGCm'C Cuts at: 0 1009 1695 Size: 1009 686 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 779 1579 1695 Size: 779 800 116 Fragments arranged by size: 800 779 116 Bpu10I CC'TnA_GC Cuts at: 0 251 1695 Size: 251 1444 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 493 1695 Size: 493 1202 BsaAI yAC'GTr Cuts at: 0 569 1551 1695 Size: 569 982 144 Fragments arranged by size: 982 569 144 BsaWI w'CCGG_w Cuts at: 0 73 296 746 1541 1695 Size: 73 223 450 795 154 Fragments arranged by size: 795 450 223 154 73 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 941 971 1577 1607 1695 Size: 941 30 606 30 88 Fragments arranged by size: 941 606 88 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 315 731 1695 Size: 315 416 964 Fragments arranged by size: 964 416 315 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 973 1695 Size: 973 722 BsiEI CG_ry'CG Cuts at: 0 9 78 1695 Size: 9 69 1617 Fragments arranged by size: 1617 69 9 BsiHKAI G_wGCw'C Cuts at: 0 127 1695 Size: 127 1568 BsmI GAATG_Cn' Cuts at: 0 987 1695 Size: 987 708 Bsp1286I G_dGCh'C Cuts at: 0 127 1009 1537 1695 Size: 127 882 528 158 Fragments arranged by size: 882 528 158 127 BspEI T'CCGG_A Cuts at: 0 746 1695 Size: 746 949 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 668 1695 Size: 668 1027 BsrBI CCG'CTC Cuts at: 0 105 255 1695 Size: 105 150 1440 Fragments arranged by size: 1440 150 105 BsrDI GCAATG_nn' Cuts at: 0 1496 1695 Size: 1496 199 BsrFI r'CCGG_y Cuts at: 0 8 165 1695 Size: 8 157 1530 Fragments arranged by size: 1530 157 8 BssHII G'CGCG_C Cuts at: 0 173 175 1695 Size: 173 2 1520 Fragments arranged by size: 1520 173 2 BstXI CCAn_nnnn'nTGG Cuts at: 0 829 1695 Size: 829 866 BstYI r'GATC_y Cuts at: 0 1061 1434 1544 1695 Size: 1061 373 110 151 Fragments arranged by size: 1061 373 151 110 Bsu36I CC'TnA_GG Cuts at: 0 113 1695 Size: 113 1582 BtsI GCAGTG_nn' Cuts at: 0 309 1695 Size: 309 1386 ClaI AT'CG_AT Cuts at: 0 545 1695 Size: 545 1150 EaeI y'GGCC_r Cuts at: 0 6 276 1695 Size: 6 270 1419 Fragments arranged by size: 1419 270 6 EagI C'GGCC_G Cuts at: 0 6 1695 Size: 6 1689 EarI CTCTTCn'nnn_ Cuts at: 0 1249 1651 1695 Size: 1249 402 44 Fragments arranged by size: 1249 402 44 EcoICRI GAG'CTC Cuts at: 0 125 1695 Size: 125 1570 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 703 779 883 1333 1475 1579 1695 Size: 703 76 104 450 142 104 116 Fragments arranged by size: 703 450 142 116 104 104 76 EcoNI CCTnn'n_nnAGG Cuts at: 0 1201 1695 Size: 1201 494 EcoO109I rG'GnC_Cy Cuts at: 0 183 1695 Size: 183 1512 EcoRV GAT'ATC Cuts at: 0 564 804 1695 Size: 564 240 891 Fragments arranged by size: 891 564 240 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1323 1398 1695 Size: 1323 75 297 Fragments arranged by size: 1323 297 75 HaeII r_GCGC'y Cuts at: 0 1320 1695 Size: 1320 375 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 43 75 226 258 952 984 1695 Size: 43 32 151 32 694 32 711 Fragments arranged by size: 711 694 151 43 32 32 32 HincII GTy'rAC Cuts at: 0 960 1695 Size: 960 735 HindIII A'AGCT_T Cuts at: 0 1075 1695 Size: 1075 620 KpnI G_GTAC'C Cuts at: 0 653 1695 Size: 653 1042 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1426 1695 Size: 1426 269 MslI CAynn'nnrTG Cuts at: 0 392 571 1162 1695 Size: 392 179 591 533 Fragments arranged by size: 591 533 392 179 NaeI GCC'GGC Cuts at: 0 167 1695 Size: 167 1528 NdeI CA'TA_TG Cuts at: 0 642 1695 Size: 642 1053 NgoMIV G'CCGG_C Cuts at: 0 165 1695 Size: 165 1530 NspI r_CATG'y Cuts at: 0 880 1695 Size: 880 815 PciI A'CATG_T Cuts at: 0 876 1695 Size: 876 819 PmlI CAC'GTG Cuts at: 0 1551 1695 Size: 1551 144 PsiI TTA'TAA Cuts at: 0 776 1695 Size: 776 919 SacI G_AGCT'C Cuts at: 0 127 1695 Size: 127 1568 ScaI AGT'ACT Cuts at: 0 424 1695 Size: 424 1271 SmaI CCC'GGG Cuts at: 0 130 637 1695 Size: 130 507 1058 Fragments arranged by size: 1058 507 130 SmlI C'TyrA_G Cuts at: 0 508 1695 Size: 508 1187 TatI w'GTAC_w Cuts at: 0 422 909 1461 1470 1695 Size: 422 487 552 9 225 Fragments arranged by size: 552 487 422 225 9 XmaI C'CCGG_G Cuts at: 0 128 635 1695 Size: 128 507 1060 Fragments arranged by size: 1060 507 128 XmnI GAAnn'nnTTC Cuts at: 0 1318 1381 1695 Size: 1318 63 314 Fragments arranged by size: 1318 314 63 Enzymes that do cut: Acc65I AcuI AfeI AflIII AhdI AleI AlfI AloI ApoI AvaI BamHI BanI BanII BbsI BclI Bme1580I BpmI Bpu10I BpuEI BsaAI BsaWI BsaXI BseRI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BssHII BstXI BstYI Bsu36I BtsI ClaI EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI HaeII Hin4I HincII HindIII KpnI MmeI MslI NaeI NdeI NgoMIV NspI PciI PmlI PsiI SacI ScaI SmaI SmlI TatI XmaI XmnI Enzymes that do not cut: AarI AatII AccI AclI AflII AgeI AlwNI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BbeI BbvCI BcgI BciVI BfrBI BglI BglII BlpI BmgBI BmrI BmtI BplI BsaI BsaBI BsaHI BseYI BsiWI BsmBI BspHI BsrGI BssSI BstAPI BstBI BstEII BstZ17I BtgI BtgZI CspCI DraI DraIII DrdI EciI EcoRI FseI FspI FspAI HpaI KasI MfeI MluI MscI MspA1I NarI NcoI NheI NotI NruI NsiI PacI PasI PflMI PfoI PmeI PpiI PpuMI PshAI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacII SalI SanDI SapI SbfI SexAI SfcI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI StyI SwaI TaqII TstI Tth111I XbaI XcmI XhoI ZraI