(Circular) (Six-base) MAPSORT of: pFN21AE2174.seq Check: 5856 from: 1 to: 5545 pFN21AE2174 5545 bp With 182 enzymes: SgfI * August 16, 2010 14:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3355 278 Size: 53 83 186 936 1819 2468 Fragments arranged by size: 2468 1819 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2888 1937 Size: 951 4594 Acc65I G'GTAC_C Cuts at: 2866 2866 Size: 5545 AclI AA'CG_TT Cuts at: 1563 3672 4045 1563 Size: 2109 373 3063 Fragments arranged by size: 3063 2109 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3608 4992 5260 1632 Size: 1976 1384 268 1917 Fragments arranged by size: 1976 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5348 AflIII A'CryG_T Cuts at: 1203 4444 5421 1203 Size: 3241 977 1327 Fragments arranged by size: 3241 1327 977 AgeI A'CCGG_T Cuts at: 4376 4376 Size: 5545 AhdI GACnn_n'nnGTC Cuts at: 2401 4274 2401 Size: 1873 3672 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5545 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2831 1184 Size: 1647 3898 AlwNI CAG_nnn'CTG Cuts at: 3288 4860 3288 Size: 1572 3973 ApaI G_GGCC'C Cuts at: 2346 2346 Size: 5545 ApaLI G'TGCA_C Cuts at: 3602 4758 3602 Size: 1156 4389 ApoI r'AATT_y Cuts at: 1493 2288 2854 3080 3232 5341 1493 Size: 795 566 226 152 2109 1697 Fragments arranged by size: 2109 1697 795 566 226 152 AseI AT'TA_AT Cuts at: 160 1032 4099 160 Size: 872 3067 1606 Fragments arranged by size: 3067 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5545 AvaI C'yCGr_G Cuts at: 1870 1943 2514 2684 2735 2870 1870 Size: 73 571 170 51 135 4545 Fragments arranged by size: 4545 571 170 135 73 51 BamHI G'GATC_C Cuts at: 2875 3242 2875 Size: 367 5178 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2080 2274 2576 2866 4321 Size: 325 217 627 293 194 302 290 1455 Cuts at: 4321 618 Size: 1842 Fragments arranged by size: 1842 1455 627 325 302 293 290 217 194 BanII G_rGCy'C Cuts at: 1391 2120 2346 2864 1391 Size: 729 226 518 4072 Fragments arranged by size: 4072 729 518 226 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4574 BbvCI CC'TCA_GC Cuts at: 2420 2420 Size: 5545 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3736 3770 1903 Size: 34 1799 34 3678 Fragments arranged by size: 3678 1799 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3437 4647 3437 Size: 1210 4335 BclI T'GATC_A Cuts at: 1548 1548 Size: 5545 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4156 136 Size: 107 122 71 631 605 2484 1525 Fragments arranged by size: 2484 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2058 2100 5540 2058 Size: 42 3440 2063 Fragments arranged by size: 3440 2063 42 BlpI GC'TnA_GC Cuts at: 2962 2962 Size: 5545 Bme1580I G_kGCm'C Cuts at: 1790 2346 2579 3606 4762 1790 Size: 556 233 1027 1156 2573 Fragments arranged by size: 2573 1156 1027 556 233 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5545 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3276 4234 442 Size: 416 53 83 399 24 1859 958 1753 Fragments arranged by size: 1859 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5545 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2139 2236 2139 Size: 97 5448 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2231 2270 4205 1239 Size: 992 39 1935 2579 Fragments arranged by size: 2579 1935 992 39 Bpu10I CC'TnA_GC Cuts at: 2420 2420 Size: 5545 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2576 2765 2783 3670 4535 4833 2576 Size: 189 18 887 865 298 3288 Fragments arranged by size: 3288 887 865 298 189 18 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2042 4208 882 Size: 617 543 2166 2219 Fragments arranged by size: 2219 2166 617 543 BsaAI yAC'GTr Cuts at: 493 1204 3336 493 Size: 711 2132 2702 Fragments arranged by size: 2702 2132 711 BsaBI GATnn'nnATC Cuts at: 5539 5539 Size: 5545 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2538 3352 3734 4369 Size: 53 83 186 936 1005 814 382 635 Cuts at: 4369 275 Size: 1451 Fragments arranged by size: 1451 1005 936 814 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2013 2146 3978 4376 4650 4797 5208 2013 Size: 133 1832 398 274 147 411 2350 Fragments arranged by size: 2350 1832 411 398 274 147 133 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2141 2171 1178 Size: 30 165 30 738 30 4552 Fragments arranged by size: 4552 738 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2153 2404 2470 1186 Size: 967 251 66 4261 Fragments arranged by size: 4261 967 251 66 BseYI C'CCAG_C Cuts at: 2193 2378 4748 5244 2193 Size: 185 2370 496 2494 Fragments arranged by size: 2494 2370 496 185 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2183 2810 1163 Size: 573 447 627 3898 Fragments arranged by size: 3898 627 573 447 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2995 3756 4784 1540 Size: 275 186 994 761 1028 2301 Fragments arranged by size: 2301 1028 994 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2619 3606 3691 4762 2619 Size: 987 85 1071 3402 Fragments arranged by size: 3402 1071 987 85 BsmI GAATG_Cn' Cuts at: 3056 3149 3056 Size: 93 5452 BsmBI CGTCTCn'nnnn_ Cuts at: 2768 2768 Size: 5545 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2120 2346 2579 2619 2864 3606 3691 Size: 399 330 226 233 40 245 742 85 Cuts at: 3691 4762 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 330 245 233 226 85 40 BspEI T'CCGG_A Cuts at: 2146 5208 2146 Size: 3062 2483 BspHI T'CATG_A Cuts at: 3434 5164 3434 Size: 1730 3815 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2900 844 Size: 2056 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3432 1394 Size: 429 1609 3507 Fragments arranged by size: 3507 1609 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4040 4214 65 Size: 1183 2792 174 1396 Fragments arranged by size: 2792 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2556 4189 4376 1700 Size: 110 746 1633 187 2869 Fragments arranged by size: 2869 1633 746 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5545 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5545 BssSI C'ACGA_G Cuts at: 3605 4617 3605 Size: 1012 4533 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5545 BstBI TT'CG_AA Cuts at: 4349 4349 Size: 5545 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5545 BstXI CCAn_nnnn'nTGG Cuts at: 2353 2353 Size: 5545 BstYI r'GATC_y Cuts at: 1972 2058 2100 2568 2875 3242 3627 3644 5096 Size: 86 42 468 307 367 385 17 1452 Cuts at: 5096 5182 5194 5540 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 468 385 367 346 307 86 86 42 17 12 BtgI C'CryG_G Cuts at: 513 1065 2368 2640 513 Size: 552 1303 272 3418 Fragments arranged by size: 3418 1303 552 272 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2644 487 Size: 683 56 1418 3388 Fragments arranged by size: 3388 1418 683 56 BtsI GCAGTG_nn' Cuts at: 2323 3061 3855 3875 2323 Size: 738 794 20 3993 Fragments arranged by size: 3993 794 738 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5545 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5510 DraI TTT'AAA Cuts at: 2849 2849 Size: 5545 DraIII CAC_nnn'GTG Cuts at: 2626 2626 Size: 5545 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4552 809 Size: 507 3236 1802 Fragments arranged by size: 3236 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2694 2992 3881 Size: 54 1425 50 275 18 864 298 889 Cuts at: 3881 5510 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 864 298 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2992 1537 Size: 275 1180 4090 Fragments arranged by size: 4090 1180 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2250 2302 2798 3475 1353 Size: 897 52 496 677 3423 Fragments arranged by size: 3423 897 677 496 52 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2044 2152 2365 4132 4506 4652 Size: 1064 138 455 108 213 1767 374 146 Cuts at: 4652 387 Size: 1280 Fragments arranged by size: 1767 1280 1064 455 374 213 146 138 108 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2231 2270 3608 4205 4992 5260 1239 Size: 393 599 39 1338 597 787 268 1524 Fragments arranged by size: 1524 1338 787 599 597 393 268 39 EcoO109I rG'GnC_Cy Cuts at: 2245 2548 2245 Size: 303 5242 EcoRI G'AATT_C Cuts at: 2854 3232 2854 Size: 378 5167 FspI TGC'GCA Cuts at: 4051 4051 Size: 5545 HaeII r_GCGC'y Cuts at: 1678 2448 2497 2648 2731 2798 4692 1678 Size: 770 49 151 83 67 1894 2531 Fragments arranged by size: 2531 1894 770 151 83 67 49 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2141 2173 2387 2419 4186 Size: 32 135 32 569 32 214 32 1767 Cuts at: 4186 4218 4260 4292 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1767 569 214 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2889 3135 669 Size: 988 281 951 246 3079 Fragments arranged by size: 3079 988 951 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5545 HpaI GTT'AAC Cuts at: 3135 3135 Size: 5545 KpnI G_GTAC'C Cuts at: 2870 2870 Size: 5545 MfeI C'AATT_G Cuts at: 3144 3144 Size: 5545 MluI A'CGCG_T Cuts at: 5421 5421 Size: 5545 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2003 2468 4659 4843 1305 Size: 698 465 2191 184 2007 Fragments arranged by size: 2191 2007 698 465 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3723 Fragments arranged by size: 3723 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3503 3862 4021 518 Size: 648 2337 359 159 2042 Fragments arranged by size: 2337 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2197 2281 2424 2751 2961 3638 4786 5031 Size: 591 84 143 327 210 677 1148 245 Cuts at: 5031 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 591 327 245 210 143 84 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4993 NdeI CA'TA_TG Cuts at: 387 387 Size: 5545 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5545 NotI GC'GGCC_GC Cuts at: 2992 2992 Size: 5545 NspI r_CATG'y Cuts at: 1132 1229 2602 2903 4448 1132 Size: 97 1373 301 1545 2229 Fragments arranged by size: 2229 1545 1373 301 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5545 PciI A'CATG_T Cuts at: 4444 4444 Size: 5545 PflMI CCAn_nnn'nTGG Cuts at: 1108 2626 1108 Size: 1518 4027 PmeI GTTT'AAAC Cuts at: 2849 2849 Size: 5545 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3614 3646 5127 5159 3614 Size: 32 1481 32 4000 Fragments arranged by size: 4000 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2245 2548 2245 Size: 303 5242 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5545 PsiI TTA'TAA Cuts at: 3115 3115 Size: 5545 PspOMI G'GGCC_C Cuts at: 2342 2342 Size: 5545 PspXI vC'TCGA_Gb Cuts at: 2735 2735 Size: 5545 PstI C_TGCA'G Cuts at: 830 2232 2897 830 Size: 1402 665 3478 Fragments arranged by size: 3478 1402 665 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5545 PvuII CAG'CTG Cuts at: 2197 2281 2197 Size: 84 5461 SalI G'TCGA_C Cuts at: 1936 2887 1936 Size: 951 4594 SapI GCTCTTCn'nnn_ Cuts at: 2798 2798 Size: 5545 SbfI CC_TGCA'GG Cuts at: 2897 2897 Size: 5545 ScaI AGT'ACT Cuts at: 2741 3793 5505 2741 Size: 1052 1712 2781 Fragments arranged by size: 2781 1712 1052 SfcI C'TryA_G Cuts at: 826 1045 2228 2893 4028 4709 4900 826 Size: 219 1183 665 1135 681 191 1471 Fragments arranged by size: 1471 1183 1135 681 665 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5545 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5545 SmaI CCC'GGG Cuts at: 1872 2516 2872 1872 Size: 644 356 4545 Fragments arranged by size: 4545 644 356 SmlI C'TyrA_G Cuts at: 820 1017 1943 2591 2735 2780 2798 3649 4550 Size: 197 926 648 144 45 18 851 901 Cuts at: 4550 4812 820 Size: 262 1553 Fragments arranged by size: 1553 926 901 851 648 262 197 144 45 18 SnaBI TAC'GTA Cuts at: 493 3336 493 Size: 2843 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5545 SphI G_CATG'C Cuts at: 1132 2903 1132 Size: 1771 3774 SspI AAT'ATT Cuts at: 5 52 3469 5 Size: 47 3417 2081 Fragments arranged by size: 3417 2081 47 StuI AGG'CCT Cuts at: 1517 2390 1517 Size: 873 4672 StyI C'CwwG_G Cuts at: 513 1065 2508 2721 2984 513 Size: 552 1443 213 263 3074 Fragments arranged by size: 3074 1443 552 263 213 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3572 3589 3742 3927 4372 325 Size: 1255 1992 17 153 185 445 1498 Fragments arranged by size: 1992 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2739 3791 5503 Size: 274 80 33 51 1443 762 1052 1712 Cuts at: 5503 96 Size: 138 Fragments arranged by size: 1712 1443 1052 762 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5505 5537 5505 Size: 32 5513 Tth111I GACn'n_nGTC Cuts at: 1316 2546 1316 Size: 1230 4315 XbaI T'CTAG_A Cuts at: 2881 2881 Size: 5545 XhoI C'TCGA_G Cuts at: 1943 2735 1943 Size: 792 4753 XmaI C'CCGG_G Cuts at: 1870 2514 2870 1870 Size: 644 356 4545 Fragments arranged by size: 4545 644 356 XmnI GAAnn'nnTTC Cuts at: 3674 3674 Size: 5545 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3353 276 Size: 53 83 186 936 1819 2468 Fragments arranged by size: 2468 1819 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI