Restriction map for pFN21AE2174
 (Linear) (Six-base) MAPSORT of: KIEE2174.seq  Check: 5700  from: 1  to: 840



KIEE2174.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:55  ..

AhdI GACnn_n'nnGTC

Cuts at:      0     396     840 
   Size:        396     444
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     826     840 
   Size:        826      14
 
ApaI G_GGCC'C

Cuts at:      0     341     840 
   Size:        341     499
 
ApoI r'AATT_y

Cuts at:      0     283     840 
   Size:        283     557
 
AvaI C'yCGr_G

Cuts at:      0     509     679     730     840 
   Size:        509     170      51     110

  Fragments arranged by size: 

                 509     170     110      51
 
BanI G'GyrC_C

Cuts at:      0      75     269     571     840 
   Size:         75     194     302     269

  Fragments arranged by size: 

                 302     269     194      75
 
BanII G_rGCy'C

Cuts at:      0     115     341     840 
   Size:        115     226     499

  Fragments arranged by size: 

                 499     226     115
 
BbvCI CC'TCA_GC

Cuts at:      0     415     840 
   Size:        415     425
 
BglII A'GATC_T

Cuts at:      0      53      95     840 
   Size:         53      42     745

  Fragments arranged by size: 

                 745      53      42
 
Bme1580I G_kGCm'C

Cuts at:      0     341     574     840 
   Size:        341     233     266

  Fragments arranged by size: 

                 341     266     233
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     134     231     840 
   Size:        134      97     609

  Fragments arranged by size: 

                 609     134      97
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     226     265     840 
   Size:        226      39     575

  Fragments arranged by size: 

                 575     226      39
 
Bpu10I CC'TnA_GC

Cuts at:      0     415     840 
   Size:        415     425
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     571     760     778     840 
   Size:        571     189      18      62

  Fragments arranged by size: 

                 571     189      62      18
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      37     840 
   Size:         37     803
 
BsaHI Gr'CG_yC

Cuts at:      0     533     840 
   Size:        533     307
 
BsaWI w'CCGG_w

Cuts at:      0       8     141     840 
   Size:          8     133     699

  Fragments arranged by size: 

                 699     133       8
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     136     166     840 
   Size:        136      30     674

  Fragments arranged by size: 

                 674     136      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     148     399     465     840 
   Size:        148     251      66     375

  Fragments arranged by size: 

                 375     251     148      66
 
BseYI C'CCAG_C

Cuts at:      0     188     373     840 
   Size:        188     185     467

  Fragments arranged by size: 

                 467     188     185
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     178     805     840 
   Size:        178     627      35

  Fragments arranged by size: 

                 627     178      35
 
BsiHKAI G_wGCw'C

Cuts at:      0     614     840 
   Size:        614     226
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     763     840 
   Size:        763      77
 
Bsp1286I G_dGCh'C

Cuts at:      0     115     341     574     614     840 
   Size:        115     226     233      40     226

  Fragments arranged by size: 

                 233     226     226     115      40
 
BspEI T'CCGG_A

Cuts at:      0     141     840 
   Size:        141     699
 
BsrFI r'CCGG_y

Cuts at:      0     551     840 
   Size:        551     289
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     348     840 
   Size:        348     492
 
BstYI r'GATC_y

Cuts at:      0      53      95     563     840 
   Size:         53      42     468     277

  Fragments arranged by size: 

                 468     277      53      42
 
BtgI C'CryG_G

Cuts at:      0     363     635     840 
   Size:        363     272     205

  Fragments arranged by size: 

                 363     272     205
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     639     840 
   Size:        639     201
 
BtsI GCAGTG_nn'

Cuts at:      0     318     840 
   Size:        318     522
 
DraIII CAC_nnn'GTG

Cuts at:      0     621     840 
   Size:        621     219
 
EaeI y'GGCC_r

Cuts at:      0     689     840 
   Size:        689     151
 
EarI CTCTTCn'nnn_

Cuts at:      0     245     297     793     840 
   Size:        245      52     496      47

  Fragments arranged by size: 

                 496     245      52      47
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      39     147     360     840 
   Size:         39     108     213     480

  Fragments arranged by size: 

                 480     213     108      39
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     226     265     840 
   Size:        226      39     575

  Fragments arranged by size: 

                 575     226      39
 
EcoO109I rG'GnC_Cy

Cuts at:      0     240     543     840 
   Size:        240     303     297

  Fragments arranged by size: 

                 303     297     240
 
HaeII r_GCGC'y

Cuts at:      0     443     492     643     726     793     840 
   Size:        443      49     151      83      67      47

  Fragments arranged by size: 

                 443     151      83      67      49      47
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     136     168     382     414     840 
   Size:        136      32     214      32     426

  Fragments arranged by size: 

                 426     214     136      32      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     463     840 
   Size:        463     377
 
MspA1I CmG'CkG

Cuts at:      0     192     276     419     746     840 
   Size:        192      84     143     327      94

  Fragments arranged by size: 

                 327     192     143      94      84
 
NspI r_CATG'y

Cuts at:      0     597     840 
   Size:        597     243
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     621     840 
   Size:        621     219
 
PpuMI rG'GwC_Cy

Cuts at:      0     240     543     840 
   Size:        240     303     297

  Fragments arranged by size: 

                 303     297     240
 
PspOMI G'GGCC_C

Cuts at:      0     337     840 
   Size:        337     503
 
PspXI vC'TCGA_Gb

Cuts at:      0     730     840 
   Size:        730     110
 
PstI C_TGCA'G

Cuts at:      0     227     840 
   Size:        227     613
 
PvuII CAG'CTG

Cuts at:      0     192     276     840 
   Size:        192      84     564

  Fragments arranged by size: 

                 564     192      84
 
SapI GCTCTTCn'nnn_

Cuts at:      0     793     840 
   Size:        793      47
 
ScaI AGT'ACT

Cuts at:      0     736     840 
   Size:        736     104
 
SfcI C'TryA_G

Cuts at:      0     223     840 
   Size:        223     617
 
SmaI CCC'GGG

Cuts at:      0     511     840 
   Size:        511     329
 
SmlI C'TyrA_G

Cuts at:      0     586     730     775     793     840 
   Size:        586     144      45      18      47

  Fragments arranged by size: 

                 586     144      47      45      18
 
StuI AGG'CCT

Cuts at:      0     385     840 
   Size:        385     455
 
StyI C'CwwG_G

Cuts at:      0     503     716     840 
   Size:        503     213     124

  Fragments arranged by size: 

                 503     213     124
 
TatI w'GTAC_w

Cuts at:      0     734     840 
   Size:        734     106
 
Tth111I GACn'n_nGTC

Cuts at:      0     541     840 
   Size:        541     299
 
XhoI C'TCGA_G

Cuts at:      0     730     840 
   Size:        730     110
 
XmaI C'CCGG_G

Cuts at:      0     509     840 
   Size:        509     331
 

 Enzymes that do cut:

     AhdI     AlfI     ApaI     ApoI     AvaI     BanI    BanII    BbvCI
    BglII Bme1580I     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI    BsmBI Bsp1286I
    BspEI    BsrFI    BstXI    BstYI     BtgI    BtgZI     BtsI   DraIII
     EaeI     EarI     EciI  Eco57MI EcoO109I    HaeII    Hin4I     MmeI
   MspA1I     NspI    PflMI    PpuMI   PspOMI    PspXI     PstI    PvuII
     SapI     ScaI     SfcI     SmaI     SmlI     StuI     StyI     TatI
  Tth111I     XhoI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AleI     AloI    AlwNI    ApaLI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BbeI     BbsI     BcgI    BciVI
     BclI    BfrBI     BglI     BlpI    BmgBI     BmrI     BmtI    BsaAI
    BsaBI    BsiEI    BsiWI     BsmI    BspHI    BspMI    BsrBI    BsrDI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I
     ClaI    CspCI     DraI     DrdI     EagI  EcoICRI    EcoNI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MscI     MslI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI     PciI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI
     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SbfI
    SexAI     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI
     SrfI     SspI     SwaI    TaqII     TstI     XbaI     XcmI     XmnI
     ZraI
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