################################## # NO CUT ENZYMES ( 78 enzymes) # ################################## Acc65I AclI AflII AflIII Aor51HI ApaI AscI AseI AsnI Asp718I AviII AvrII BbrPI BbvCI BfrI BglII BlnI BsaBI Bsp1407I BspDI BspHI BspLU11I BsrGI BssHII BssSI Bst1107I BstZ17I ClaI CpoI DraI DraIII DrdI Eco47III EcoNI EcoRI EcoRV EcoT22I FseI FspI HindIII HpaI KpnI MamI MfeI MluI MseI MunI NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI SbfI SgrAI SnaBI SpeI Sse8387I SspBI SspI SwaI Tru9I XbaI XhoI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AatII 385 GACGTC GACGT^C AccIII 1550 TCCGGA T^CCGGA AgeI 770 ACCGGT A^CCGGT AhdI 655 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1831 GTGCAC G^TGCAC ApaLI 1831 GTGCAC G^TGCAC Asp700I 253 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 655 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BbeI 554 GGCGCC GGCGC^C BcgI 680 GCAAGGCCGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 148 GTATCC GTATCC(6/5) BfaI 2230 CTAG C^TAG BsaAI 1015 TACGTG [CT]AC^GT[GA] BseAI 1550 TCCGGA T^CCGGA BseRI 2239 GAGGAG GAGGAG(10/8) BsiWI 38 CGTACG C^GTACG BsmI 1892 GCATTC GAATGC(1/-1) BspEI 1550 TCCGGA T^CCGGA BspMI 1916 ACCTGC ACCTGC(4/8) BstBI 260 TTCGAA TT^CGAA BstEII 198 GGTGACC G^GT[ACGT]ACC BstPI 198 GGTGACC G^GT[ACGT]ACC DraII 542 AGGCCCT [GA]G^G[ACGT]CC[CT] Eam1105I 655 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 253 GAAGAG CTCTTC(1/4) EcoO109I 542 AGGCCCT [GA]G^G[ACGT]CC[CT] EcoO65I 198 GGTGACC G^GT[ACGT]ACC HincII 745 GTCAAC GT[CT]^[GA]AC HindII 745 GTCAAC GT[CT]^[GA]AC KasI 554 GGCGCC G^GCGCC Ksp632I 253 GAAGAG CTCTTC(1/4) KspI 1773 CCGCGG CCGC^GG MaeI 2230 CTAG C^TAG MroI 1550 TCCGGA T^CCGGA NarI 554 GGCGCC GG^CGCC NspV 260 TTCGAA TT^CGAA PinAI 770 ACCGGT A^CCGGT SacII 1773 CCGCGG CCGC^GG SapI 253 GAAGAGC GCTCTTC(1/4) ScaI 2153 AGTACT AGT^ACT SfiI 675 GGCCCGCAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 554 GGCGCC GGC^GCC SfuI 260 TTCGAA TT^CGAA SphI 600 GCATGC GCATG^C StuI 1757 AGGCCT AGG^CCT XcmI 1730 CCAGTGTCGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 253 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AccI 28 GTCTAC GT^[AC][GT]AC 1876 GTCTAC AcsI 478 GAATTT [GA]^AATT[CT] 1519 AAATTT ApoI 478 GAATTT [GA]^AATT[CT] 1519 AAATTT AspI 718 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC 1377 GACACTGTC BamHI 1667 GGATCC G^GATCC 2087 GGATCC BclI 164 TGATCA T^GATCA 1478 TGATCA BlpI 2214 GCTCAGC GC^T[ACGT]AGC 2269 GCTCAGC Bpu1102I 2214 GCTCAGC GC^T[ACGT]AGC 2269 GCTCAGC BsiEI 974 CGGCCG CG[GA][CT]^CG 1485 CGGCCG BsmBI 898 GAGACG CGTCTC(1/5) 2242 GAGACG BsrBI 1186 CCGCTC CCGCTC(-3/-3) 1074 GAGCGG BsrDI 1281 CATTGC GCAATG(2/0) 1493 GCAATG BsrFI 770 ACCGGT [GA]^CCGG[CT] 854 ACCGGC BstAPI 834 GCAGCGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1041 GCATGTGGTGC CelII 2214 GCTCAGC GC^T[ACGT]AGC 2269 GCTCAGC Cfr10I 770 ACCGGT [GA]^CCGG[CT] 854 ACCGGC EagI 974 CGGCCG C^GGCCG 1485 CGGCCG EclXI 974 CGGCCG C^GGCCG 1485 CGGCCG Eco52I 974 CGGCCG C^GGCCG 1485 CGGCCG FbaI 164 TGATCA T^GATCA 1478 TGATCA MslI 490 CATGGGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 601 CATGCCAATG NcoI 489 CCATGG C^CATGG 1430 CCATGG PflFI 718 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC 1377 GACACTGTC PflMI 1626 CCAGTACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1983 CCAGTGTGTGG PleI 733 GACTC GAGTC(4/5) 113 GAGTC PvuII 1305 CAGCTG CAG^CTG 2197 CAGCTG SacI 79 GAGCTC GAGCT^C 327 GAGCTC SexAI 656 ACCTGGT A^CC[AT]GGT 1499 ACCAGGT Tsp509I 479 AATT ^AATT 1520 AATT Tth111I 718 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC 1377 GACACTGTC Van91I 1626 CCAGTACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1983 CCAGTGTGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 102 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI AocI AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BcnI BglI BmyI BpmI BpuAI BsaHI BsaI BsaJI BsaWI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DsaI EaeI Eco81I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StyI TaqI TfiI TseI Tsp45I TspRI TthHB8I XhoII XmaCI XmaI