(Circular) (Six-base) MAPSORT of: pFN21AE2086.seq Check: 928 from: 1 to: 6985 pFN21AE2086 6985 bp With 182 enzymes: SgfI * August 16, 2010 14:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2394 4795 278 Size: 53 83 186 936 858 2401 2468 Fragments arranged by size: 2468 2401 936 858 186 83 53 AccI GT'mk_AC Cuts at: 1937 2034 3882 4328 1937 Size: 97 1848 446 4594 Fragments arranged by size: 4594 1848 446 97 Acc65I G'GTAC_C Cuts at: 4306 4306 Size: 6985 AclI AA'CG_TT Cuts at: 1563 5112 5485 1563 Size: 3549 373 3063 Fragments arranged by size: 3549 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2564 3292 5048 6432 6700 1632 Size: 932 728 1756 1384 268 1917 Fragments arranged by size: 1917 1756 1384 932 728 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6788 AflIII A'CryG_T Cuts at: 1203 5884 6861 1203 Size: 4681 977 1327 Fragments arranged by size: 4681 1327 977 AgeI A'CCGG_T Cuts at: 2775 5816 2775 Size: 3041 3944 AhdI GACnn_n'nnGTC Cuts at: 2665 5714 2665 Size: 3049 3936 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6985 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3484 1184 Size: 2300 4685 AlwNI CAG_nnn'CTG Cuts at: 2845 2963 3126 4728 6300 2845 Size: 118 163 1602 1572 3530 Fragments arranged by size: 3530 1602 1572 163 118 ApaLI G'TGCA_C Cuts at: 3836 5042 6198 3836 Size: 1206 1156 4623 Fragments arranged by size: 4623 1206 1156 ApoI r'AATT_y Cuts at: 1493 2483 3524 4294 4520 4672 6781 1493 Size: 990 1041 770 226 152 2109 1697 Fragments arranged by size: 2109 1697 1041 990 770 226 152 AseI AT'TA_AT Cuts at: 160 1032 5539 160 Size: 872 4507 1606 Fragments arranged by size: 4507 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6985 AvaI C'yCGr_G Cuts at: 1870 1943 2060 2898 3534 3804 4310 1870 Size: 73 117 838 636 270 506 4545 Fragments arranged by size: 4545 838 636 506 270 117 73 BamHI G'GATC_C Cuts at: 3672 4092 4315 4682 3672 Size: 420 223 367 5975 Fragments arranged by size: 5975 420 367 223 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2138 2506 2559 2571 2778 Size: 325 217 627 351 368 53 12 207 Cuts at: 2778 3725 4306 5761 618 Size: 947 581 1455 1842 Fragments arranged by size: 1842 1455 947 627 581 368 351 325 217 207 53 12 BanII G_rGCy'C Cuts at: 1391 2028 2088 2336 3065 3264 4304 1391 Size: 637 60 248 729 199 1040 4072 Fragments arranged by size: 4072 1040 729 637 248 199 60 BbeI G_GCGC'C Cuts at: 2563 2563 Size: 6985 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2153 2692 2813 928 Size: 971 254 539 121 5100 Fragments arranged by size: 5100 971 539 254 121 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2674 2708 5176 5210 1903 Size: 34 737 34 2468 34 3678 Fragments arranged by size: 3678 2468 737 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2164 4877 6087 2164 Size: 2713 1210 3062 Fragments arranged by size: 3062 2713 1210 BclI T'GATC_A Cuts at: 1548 2169 3483 1548 Size: 621 1314 5050 Fragments arranged by size: 5050 1314 621 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2687 2918 3329 Size: 107 122 71 631 605 1015 231 411 Cuts at: 3329 5596 136 Size: 2267 1525 Fragments arranged by size: 2267 1525 1015 631 605 411 231 122 107 71 BglII A'GATC_T Cuts at: 6980 6980 Size: 6985 BlpI GC'TnA_GC Cuts at: 4220 4275 4402 4220 Size: 55 127 6803 Fragments arranged by size: 6803 127 55 Bme1580I G_kGCm'C Cuts at: 1790 2511 2576 3840 5046 6202 1790 Size: 721 65 1264 1206 1156 2573 Fragments arranged by size: 2573 1264 1206 1156 721 65 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6985 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3982 4716 5674 Size: 416 53 83 399 24 2565 734 958 Cuts at: 5674 442 Size: 1753 Fragments arranged by size: 2565 1753 958 734 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6985 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2086 2149 2086 Size: 63 6922 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2144 2219 2813 3035 3182 3599 3767 4247 Size: 905 75 594 222 147 417 168 480 Cuts at: 4247 5645 1239 Size: 1398 2579 Fragments arranged by size: 2579 1398 905 594 480 417 222 168 147 75 Bpu10I CC'TnA_GC Cuts at: 2816 3077 2816 Size: 261 6724 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2209 3698 3887 5110 5975 6273 2209 Size: 1489 189 1223 865 298 2921 Fragments arranged by size: 2921 1489 1223 865 298 189 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2789 3524 3707 5648 882 Size: 617 1290 735 183 1941 2219 Fragments arranged by size: 2219 1941 1290 735 617 183 BsaAI yAC'GTr Cuts at: 493 1204 3022 4776 493 Size: 711 1818 1754 2702 Fragments arranged by size: 2702 1818 1754 711 BsaBI GATnn'nnATC Cuts at: 6979 6979 Size: 6985 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2103 2391 2560 2863 Size: 53 83 186 936 570 288 169 303 Cuts at: 2863 2910 3321 3870 4792 5174 5809 275 Size: 47 411 549 922 382 635 1451 Fragments arranged by size: 1451 936 922 635 570 549 411 382 303 288 186 169 83 53 47 BsaWI w'CCGG_w Cuts at: 2775 3141 3555 5418 5816 6090 6237 6648 2775 Size: 366 414 1863 398 274 147 411 3112 Fragments arranged by size: 3112 1863 414 411 398 366 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6760 Fragments arranged by size: 6760 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 4259 1186 Size: 3073 3912 BseYI C'CCAG_C Cuts at: 3090 4204 6188 6684 3090 Size: 1114 1984 496 3391 Fragments arranged by size: 3391 1984 1114 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3062 3074 3287 1163 Size: 573 1326 12 213 4861 Fragments arranged by size: 4861 1326 573 213 12 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2982 3493 4435 5196 6224 1540 Size: 275 186 981 511 942 761 1028 2301 Fragments arranged by size: 2301 1028 981 942 761 511 275 186 BsiHKAI G_wGCw'C Cuts at: 2088 2336 2817 3647 3840 4104 5046 5131 6202 Size: 248 481 830 193 264 942 85 1071 Cuts at: 6202 2088 Size: 2871 Fragments arranged by size: 2871 1071 942 830 481 264 248 193 85 BsiWI C'GTAC_G Cuts at: 2043 2043 Size: 6985 BsmI GAATG_Cn' Cuts at: 3897 4496 4589 3897 Size: 599 93 6293 Fragments arranged by size: 6293 599 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2897 4241 2897 Size: 1344 5641 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2028 2088 2336 2511 2576 2817 3065 Size: 399 238 60 248 175 65 241 248 Cuts at: 3065 3264 3647 3840 4104 4304 5046 5131 6202 Size: 199 383 193 264 200 742 85 1071 Cuts at: 6202 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 383 264 248 248 241 238 200 199 193 175 85 65 60 BspEI T'CCGG_A Cuts at: 3555 6648 3555 Size: 3093 3892 BspHI T'CATG_A Cuts at: 4874 6604 4874 Size: 1730 5255 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3930 4340 844 Size: 3086 410 3489 Fragments arranged by size: 3489 3086 410 BsrBI CCG'CTC Cuts at: 1394 1823 3081 3193 4872 1394 Size: 429 1258 112 1679 3507 Fragments arranged by size: 3507 1679 1258 429 112 BsrDI GCAATG_nn' Cuts at: 65 1248 3285 3505 5480 5654 65 Size: 1183 2037 220 1975 174 1396 Fragments arranged by size: 2037 1975 1396 1183 220 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2775 2859 5629 5816 1700 Size: 110 965 84 2770 187 2869 Fragments arranged by size: 2869 2770 965 187 110 84 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6985 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6985 BssSI C'ACGA_G Cuts at: 5045 6057 5045 Size: 1012 5973 BstAPI GCAn_nnn'nTGC Cuts at: 794 2845 3052 794 Size: 2051 207 4727 Fragments arranged by size: 4727 2051 207 BstBI TT'CG_AA Cuts at: 2266 5789 2266 Size: 3523 3462 BstEII G'GTnAC_C Cuts at: 1184 2203 1184 Size: 1019 5966 BstXI CCAn_nnnn'nTGG Cuts at: 2617 2677 3415 3457 2617 Size: 60 738 42 6145 Fragments arranged by size: 6145 738 60 42 BstYI r'GATC_y Cuts at: 1972 3411 3672 4092 4315 4682 5067 5084 6536 Size: 1439 261 420 223 367 385 17 1452 Cuts at: 6536 6622 6634 6980 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1439 420 385 367 346 261 223 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2134 2361 3002 2134 Size: 227 641 6117 Fragments arranged by size: 6117 641 227 BtgI C'CryG_G Cuts at: 513 1065 2494 3435 3493 3648 3778 4060 4131 Size: 552 1429 941 58 155 130 282 71 Cuts at: 4131 513 Size: 3367 Fragments arranged by size: 3367 1429 941 552 282 155 130 71 58 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6246 Fragments arranged by size: 6246 683 56 BtsI GCAGTG_nn' Cuts at: 2464 2964 4501 5295 5315 2464 Size: 500 1537 794 20 4134 Fragments arranged by size: 4134 1537 794 500 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6985 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6950 DraI TTT'AAA Cuts at: 4289 4289 Size: 6985 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5992 809 Size: 507 4676 1802 Fragments arranged by size: 4676 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2919 2979 3330 Size: 54 1425 50 275 18 1089 60 351 Cuts at: 3330 3490 3510 3775 4152 4432 5321 6950 8 Size: 160 20 265 377 280 889 1629 43 Fragments arranged by size: 1629 1425 1089 889 377 351 280 275 265 160 60 54 50 43 20 18 EagI C'GGCC_G Cuts at: 1537 1812 2979 3490 4432 1537 Size: 275 1167 511 942 4090 Fragments arranged by size: 4090 1167 942 511 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2253 4915 1353 Size: 900 2662 3423 Fragments arranged by size: 3423 2662 900 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5572 5946 6092 387 Size: 1064 138 3983 374 146 1280 Fragments arranged by size: 3983 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2086 2334 2086 Size: 248 6737 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2144 2219 2564 2813 3035 3182 3292 Size: 393 512 75 345 249 222 147 110 Cuts at: 3292 3599 3767 4247 5048 5645 6432 6700 1239 Size: 307 168 480 801 597 787 268 1524 Fragments arranged by size: 1524 801 787 597 512 480 393 345 307 268 249 222 168 147 110 75 EcoO109I rG'GnC_Cy Cuts at: 2548 2548 Size: 6985 EcoRI G'AATT_C Cuts at: 4294 4672 4294 Size: 378 6607 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3721 3721 Size: 6985 FspI TGC'GCA Cuts at: 5491 5491 Size: 6985 HaeII r_GCGC'y Cuts at: 1678 2563 2845 2895 6132 1678 Size: 885 282 50 3237 2531 Fragments arranged by size: 3237 2531 885 282 50 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2176 2208 2583 2615 2651 Size: 32 135 32 604 32 375 32 36 Cuts at: 2651 2683 5626 5658 5700 5732 1373 Size: 32 2943 32 42 32 2626 Fragments arranged by size: 2943 2626 604 375 135 42 36 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2752 4329 4575 669 Size: 988 281 814 1577 246 3079 Fragments arranged by size: 3079 1577 988 814 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6985 HpaI GTT'AAC Cuts at: 4575 4575 Size: 6985 KasI G'GCGC_C Cuts at: 2559 2559 Size: 6985 KpnI G_GTAC'C Cuts at: 4310 4310 Size: 6985 MfeI C'AATT_G Cuts at: 4584 4584 Size: 6985 MluI A'CGCG_T Cuts at: 6861 6861 Size: 6985 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6099 6283 1305 Size: 4794 184 2007 Fragments arranged by size: 4794 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2921 3332 3512 4154 10 Size: 54 1425 343 1089 411 180 642 2841 Fragments arranged by size: 2841 1425 1089 642 411 343 180 54 MslI CAynn'nnrTG Cuts at: 518 1166 2499 2610 4943 5302 5461 518 Size: 648 1333 111 2333 359 159 2042 Fragments arranged by size: 2333 2042 1333 648 359 159 111 MspA1I CmG'CkG Cuts at: 1606 2197 2519 3312 3394 3590 3780 4089 4204 Size: 591 322 793 82 196 190 309 115 Cuts at: 4204 4401 5078 6226 6471 1606 Size: 197 677 1148 245 2120 Fragments arranged by size: 2120 1148 793 677 591 322 309 245 197 196 190 115 82 NarI GG'CG_CC Cuts at: 2560 2560 Size: 6985 NcoI C'CATG_G Cuts at: 513 1065 2494 3435 513 Size: 552 1429 941 4063 Fragments arranged by size: 4063 1429 941 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6985 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6985 NotI GC'GGCC_GC Cuts at: 4432 4432 Size: 6985 NspI r_CATG'y Cuts at: 1132 1229 2080 2609 3050 4343 5888 1132 Size: 97 851 529 441 1293 1545 2229 Fragments arranged by size: 2229 1545 1293 851 529 441 97 PasI CC'CwG_GG Cuts at: 1348 2551 1348 Size: 1203 5782 PciI A'CATG_T Cuts at: 5884 5884 Size: 6985 PflMI CCAn_nnn'nTGG Cuts at: 1108 3637 3994 1108 Size: 2529 357 4099 Fragments arranged by size: 4099 2529 357 PfoI T'CCnGG_A Cuts at: 2121 2640 3414 4095 2121 Size: 519 774 681 5011 Fragments arranged by size: 5011 774 681 519 PmeI GTTT'AAAC Cuts at: 4289 4289 Size: 6985 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5054 5086 6567 6599 5054 Size: 32 1481 32 5440 Fragments arranged by size: 5440 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6985 PsiI TTA'TAA Cuts at: 4555 4555 Size: 6985 PstI C_TGCA'G Cuts at: 830 2805 2952 4257 4337 830 Size: 1975 147 1305 80 3478 Fragments arranged by size: 3478 1975 1305 147 80 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6985 PvuII CAG'CTG Cuts at: 3312 4204 3312 Size: 892 6093 SacI G_AGCT'C Cuts at: 2088 2336 2088 Size: 248 6737 SacII CC_GC'GG Cuts at: 3781 3781 Size: 6985 SalI G'TCGA_C Cuts at: 1936 4327 1936 Size: 2391 4594 SapI GCTCTTCn'nnn_ Cuts at: 2253 2253 Size: 6985 SbfI CC_TGCA'GG Cuts at: 4337 4337 Size: 6985 ScaI AGT'ACT Cuts at: 4160 5233 6945 4160 Size: 1073 1712 4200 Fragments arranged by size: 4200 1712 1073 SexAI A'CCwGG_T Cuts at: 2661 3504 2661 Size: 843 6142 SfcI C'TryA_G Cuts at: 826 1045 2801 2948 4253 4333 5468 6149 6340 Size: 219 1756 147 1305 80 1135 681 191 Cuts at: 6340 826 Size: 1471 Fragments arranged by size: 1756 1471 1305 1135 681 219 191 147 80 SfiI GGCCn_nnn'nGGCC Cuts at: 2687 2687 Size: 6985 SfoI GGC'GCC Cuts at: 2561 2561 Size: 6985 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6985 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6985 SmaI CCC'GGG Cuts at: 1872 2900 3536 3806 4312 1872 Size: 1028 636 270 506 4545 Fragments arranged by size: 4545 1028 636 506 270 SmlI C'TyrA_G Cuts at: 820 1017 1943 2224 3677 3902 5089 5990 6252 Size: 197 926 281 1453 225 1187 901 262 Cuts at: 6252 820 Size: 1553 Fragments arranged by size: 1553 1453 1187 926 901 281 262 225 197 SnaBI TAC'GTA Cuts at: 493 4776 493 Size: 4283 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6985 SphI G_CATG'C Cuts at: 1132 2609 4343 1132 Size: 1477 1734 3774 Fragments arranged by size: 3774 1734 1477 SspI AAT'ATT Cuts at: 5 52 4909 5 Size: 47 4857 2081 Fragments arranged by size: 4857 2081 47 StuI AGG'CCT Cuts at: 1517 3764 1517 Size: 2247 4738 StyI C'CwwG_G Cuts at: 513 1065 2106 2494 2670 3435 4424 513 Size: 552 1041 388 176 765 989 3074 Fragments arranged by size: 3074 1041 989 765 552 388 176 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5012 5029 5182 5367 5812 325 Size: 1255 3432 17 153 185 445 1498 Fragments arranged by size: 3432 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3633 4158 5231 Size: 274 80 33 51 1443 1656 525 1073 Cuts at: 5231 6943 96 Size: 1712 138 Fragments arranged by size: 1712 1656 1443 1073 525 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4170 4202 6945 6977 4170 Size: 32 2743 32 4178 Fragments arranged by size: 4178 2743 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2726 3385 1316 Size: 1410 659 4916 Fragments arranged by size: 4916 1410 659 XbaI T'CTAG_A Cuts at: 4321 4321 Size: 6985 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3742 3742 Size: 6985 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6985 XmaI C'CCGG_G Cuts at: 1870 2898 3534 3804 4310 1870 Size: 1028 636 270 506 4545 Fragments arranged by size: 4545 1028 636 506 270 XmnI GAAnn'nnTTC Cuts at: 2262 5114 2262 Size: 2852 4133 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2392 4793 276 Size: 53 83 186 936 858 2401 2468 Fragments arranged by size: 2468 2401 936 858 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BstZ17I DraIII EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SanDI SrfI SwaI