(Linear) (Six-base) MAPSORT of: KIEE2086.seq Check: 3359 from: 1 to: 2280 KIEE2086.orf With 182 enzymes: SgfI * July 15, 2010 18:54 .. AatII G_ACGT'C Cuts at: 0 389 2280 Size: 389 1891 AccI GT'mk_AC Cuts at: 0 29 1877 2280 Size: 29 1848 403 Fragments arranged by size: 1848 403 29 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 559 1287 2280 Size: 559 728 993 Fragments arranged by size: 993 728 559 AgeI A'CCGG_T Cuts at: 0 770 2280 Size: 770 1510 AhdI GACnn_n'nnGTC Cuts at: 0 660 2280 Size: 660 1620 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1479 2280 Size: 1479 801 AlwNI CAG_nnn'CTG Cuts at: 0 840 958 1121 2280 Size: 840 118 163 1159 Fragments arranged by size: 1159 840 163 118 ApaLI G'TGCA_C Cuts at: 0 1831 2280 Size: 1831 449 ApoI r'AATT_y Cuts at: 0 478 1519 2280 Size: 478 1041 761 Fragments arranged by size: 1041 761 478 AvaI C'yCGr_G Cuts at: 0 55 893 1529 1799 2280 Size: 55 838 636 270 481 Fragments arranged by size: 838 636 481 270 55 BamHI G'GATC_C Cuts at: 0 1667 2087 2280 Size: 1667 420 193 Fragments arranged by size: 1667 420 193 BanI G'GyrC_C Cuts at: 0 133 501 554 566 773 1720 2280 Size: 133 368 53 12 207 947 560 Fragments arranged by size: 947 560 368 207 133 53 12 BanII G_rGCy'C Cuts at: 0 23 83 331 1060 1259 2280 Size: 23 60 248 729 199 1021 Fragments arranged by size: 1021 729 248 199 60 23 BbeI G_GCGC'C Cuts at: 0 558 2280 Size: 558 1722 BbsI GAAGACnn'nnnn_ Cuts at: 0 148 687 808 2280 Size: 148 539 121 1472 Fragments arranged by size: 1472 539 148 121 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 669 703 2280 Size: 669 34 1577 Fragments arranged by size: 1577 669 34 BciVI GTATCCnnnnn_n' Cuts at: 0 159 2280 Size: 159 2121 BclI T'GATC_A Cuts at: 0 164 1478 2280 Size: 164 1314 802 Fragments arranged by size: 1314 802 164 BglI GCCn_nnn'nGGC Cuts at: 0 682 913 1324 2280 Size: 682 231 411 956 Fragments arranged by size: 956 682 411 231 BlpI GC'TnA_GC Cuts at: 0 2215 2270 2280 Size: 2215 55 10 Fragments arranged by size: 2215 55 10 Bme1580I G_kGCm'C Cuts at: 0 506 571 1835 2280 Size: 506 65 1264 445 Fragments arranged by size: 1264 506 445 65 BmrI ACTGGGnnnn_n' Cuts at: 0 1977 2280 Size: 1977 303 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 81 144 2280 Size: 81 63 2136 Fragments arranged by size: 2136 81 63 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 139 214 808 1030 1177 1594 1762 2242 Size: 139 75 594 222 147 417 168 480 Cuts at: 2242 2280 Size: 38 Fragments arranged by size: 594 480 417 222 168 147 139 75 38 Bpu10I CC'TnA_GC Cuts at: 0 811 1072 2280 Size: 811 261 1208 Fragments arranged by size: 1208 811 261 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 204 1693 1882 2280 Size: 204 1489 189 398 Fragments arranged by size: 1489 398 204 189 BsaI GGTCTCn'nnnn_ Cuts at: 0 784 1519 1702 2280 Size: 784 735 183 578 Fragments arranged by size: 784 735 578 183 BsaAI yAC'GTr Cuts at: 0 1017 2280 Size: 1017 1263 BsaHI Gr'CG_yC Cuts at: 0 98 386 555 858 905 1316 1865 2280 Size: 98 288 169 303 47 411 549 415 Fragments arranged by size: 549 415 411 303 288 169 98 47 BsaWI w'CCGG_w Cuts at: 0 770 1136 1550 2280 Size: 770 366 414 730 Fragments arranged by size: 770 730 414 366 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 2254 2280 Size: 2254 26 BseYI C'CCAG_C Cuts at: 0 1085 2199 2280 Size: 1085 1114 81 Fragments arranged by size: 1114 1085 81 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1057 1069 1282 2280 Size: 1057 12 213 998 Fragments arranged by size: 1057 998 213 12 BsiEI CG_ry'CG Cuts at: 0 977 1488 2280 Size: 977 511 792 Fragments arranged by size: 977 792 511 BsiHKAI G_wGCw'C Cuts at: 0 83 331 812 1642 1835 2099 2280 Size: 83 248 481 830 193 264 181 Fragments arranged by size: 830 481 264 248 193 181 83 BsiWI C'GTAC_G Cuts at: 0 38 2280 Size: 38 2242 BsmI GAATG_Cn' Cuts at: 0 1892 2280 Size: 1892 388 BsmBI CGTCTCn'nnnn_ Cuts at: 0 892 2236 2280 Size: 892 1344 44 Fragments arranged by size: 1344 892 44 Bsp1286I G_dGCh'C Cuts at: 0 23 83 331 506 571 812 1060 1259 Size: 23 60 248 175 65 241 248 199 Cuts at: 1259 1642 1835 2099 2280 Size: 383 193 264 181 Fragments arranged by size: 383 264 248 248 241 199 193 181 175 65 60 23 BspEI T'CCGG_A Cuts at: 0 1550 2280 Size: 1550 730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1925 2280 Size: 1925 355 BsrBI CCG'CTC Cuts at: 0 1076 1188 2280 Size: 1076 112 1092 Fragments arranged by size: 1092 1076 112 BsrDI GCAATG_nn' Cuts at: 0 1280 1500 2280 Size: 1280 220 780 Fragments arranged by size: 1280 780 220 BsrFI r'CCGG_y Cuts at: 0 770 854 2280 Size: 770 84 1426 Fragments arranged by size: 1426 770 84 BstAPI GCAn_nnn'nTGC Cuts at: 0 840 1047 2280 Size: 840 207 1233 Fragments arranged by size: 1233 840 207 BstBI TT'CG_AA Cuts at: 0 261 2280 Size: 261 2019 BstEII G'GTnAC_C Cuts at: 0 198 2280 Size: 198 2082 BstXI CCAn_nnnn'nTGG Cuts at: 0 612 672 1410 1452 2280 Size: 612 60 738 42 828 Fragments arranged by size: 828 738 612 60 42 BstYI r'GATC_y Cuts at: 0 1406 1667 2087 2280 Size: 1406 261 420 193 Fragments arranged by size: 1406 420 261 193 Bsu36I CC'TnA_GG Cuts at: 0 129 356 997 2280 Size: 129 227 641 1283 Fragments arranged by size: 1283 641 227 129 BtgI C'CryG_G Cuts at: 0 489 1430 1488 1643 1773 2055 2126 2280 Size: 489 941 58 155 130 282 71 154 Fragments arranged by size: 941 489 282 155 154 130 71 58 BtsI GCAGTG_nn' Cuts at: 0 459 959 2280 Size: 459 500 1321 Fragments arranged by size: 1321 500 459 EaeI y'GGCC_r Cuts at: 0 914 974 1325 1485 1505 1770 2147 2280 Size: 914 60 351 160 20 265 377 133 Fragments arranged by size: 914 377 351 265 160 133 60 20 EagI C'GGCC_G Cuts at: 0 974 1485 2280 Size: 974 511 795 Fragments arranged by size: 974 795 511 EarI CTCTTCn'nnn_ Cuts at: 0 248 2280 Size: 248 2032 EcoICRI GAG'CTC Cuts at: 0 81 329 2280 Size: 81 248 1951 Fragments arranged by size: 1951 248 81 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 139 214 559 808 1030 1177 1287 1594 Size: 139 75 345 249 222 147 110 307 Cuts at: 1594 1762 2242 2280 Size: 168 480 38 Fragments arranged by size: 480 345 307 249 222 168 147 139 110 75 38 EcoO109I rG'GnC_Cy Cuts at: 0 543 2280 Size: 543 1737 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1716 2280 Size: 1716 564 HaeII r_GCGC'y Cuts at: 0 558 840 890 2280 Size: 558 282 50 1390 Fragments arranged by size: 1390 558 282 50 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 171 203 578 610 646 678 2280 Size: 171 32 375 32 36 32 1602 Fragments arranged by size: 1602 375 171 36 32 32 32 HincII GTy'rAC Cuts at: 0 747 2280 Size: 747 1533 KasI G'GCGC_C Cuts at: 0 554 2280 Size: 554 1726 MscI TGG'CCA Cuts at: 0 916 1327 1507 2149 2280 Size: 916 411 180 642 131 Fragments arranged by size: 916 642 411 180 131 MslI CAynn'nnrTG Cuts at: 0 494 605 2280 Size: 494 111 1675 Fragments arranged by size: 1675 494 111 MspA1I CmG'CkG Cuts at: 0 192 514 1307 1389 1585 1775 2084 2199 Size: 192 322 793 82 196 190 309 115 Cuts at: 2199 2280 Size: 81 Fragments arranged by size: 793 322 309 196 192 190 115 82 81 NarI GG'CG_CC Cuts at: 0 555 2280 Size: 555 1725 NcoI C'CATG_G Cuts at: 0 489 1430 2280 Size: 489 941 850 Fragments arranged by size: 941 850 489 NspI r_CATG'y Cuts at: 0 75 604 1045 2280 Size: 75 529 441 1235 Fragments arranged by size: 1235 529 441 75 PasI CC'CwG_GG Cuts at: 0 546 2280 Size: 546 1734 PflMI CCAn_nnn'nTGG Cuts at: 0 1632 1989 2280 Size: 1632 357 291 Fragments arranged by size: 1632 357 291 PfoI T'CCnGG_A Cuts at: 0 116 635 1409 2090 2280 Size: 116 519 774 681 190 Fragments arranged by size: 774 681 519 190 116 PstI C_TGCA'G Cuts at: 0 800 947 2252 2280 Size: 800 147 1305 28 Fragments arranged by size: 1305 800 147 28 PvuII CAG'CTG Cuts at: 0 1307 2199 2280 Size: 1307 892 81 Fragments arranged by size: 1307 892 81 SacI G_AGCT'C Cuts at: 0 83 331 2280 Size: 83 248 1949 Fragments arranged by size: 1949 248 83 SacII CC_GC'GG Cuts at: 0 1776 2280 Size: 1776 504 SapI GCTCTTCn'nnn_ Cuts at: 0 248 2280 Size: 248 2032 ScaI AGT'ACT Cuts at: 0 2155 2280 Size: 2155 125 SexAI A'CCwGG_T Cuts at: 0 656 1499 2280 Size: 656 843 781 Fragments arranged by size: 843 781 656 SfcI C'TryA_G Cuts at: 0 796 943 2248 2280 Size: 796 147 1305 32 Fragments arranged by size: 1305 796 147 32 SfiI GGCCn_nnn'nGGCC Cuts at: 0 682 2280 Size: 682 1598 SfoI GGC'GCC Cuts at: 0 556 2280 Size: 556 1724 SmaI CCC'GGG Cuts at: 0 895 1531 1801 2280 Size: 895 636 270 479 Fragments arranged by size: 895 636 479 270 SmlI C'TyrA_G Cuts at: 0 219 1672 1897 2280 Size: 219 1453 225 383 Fragments arranged by size: 1453 383 225 219 SphI G_CATG'C Cuts at: 0 604 2280 Size: 604 1676 StuI AGG'CCT Cuts at: 0 1759 2280 Size: 1759 521 StyI C'CwwG_G Cuts at: 0 101 489 665 1430 2280 Size: 101 388 176 765 850 Fragments arranged by size: 850 765 388 176 101 TatI w'GTAC_w Cuts at: 0 1628 2153 2280 Size: 1628 525 127 Fragments arranged by size: 1628 525 127 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2165 2197 2280 Size: 2165 32 83 Fragments arranged by size: 2165 83 32 Tth111I GACn'n_nGTC Cuts at: 0 721 1380 2280 Size: 721 659 900 Fragments arranged by size: 900 721 659 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1737 2280 Size: 1737 543 XmaI C'CCGG_G Cuts at: 0 893 1529 1799 2280 Size: 893 636 270 481 Fragments arranged by size: 893 636 481 270 XmnI GAAnn'nnTTC Cuts at: 0 257 2280 Size: 257 2023 ZraI GAC'GTC Cuts at: 0 387 2280 Size: 387 1893 Enzymes that do cut: AatII AccI AcuI AgeI AhdI AlfI AlwNI ApaLI ApoI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtsI EaeI EagI EarI EcoICRI Eco57MI EcoO109I FalI HaeII Hin4I HincII KasI MscI MslI MspA1I NarI NcoI NspI PasI PflMI PfoI PstI PvuII SacI SacII SapI ScaI SexAI SfcI SfiI SfoI SmaI SmlI SphI StuI StyI TatI TstI Tth111I XcmI XmaI XmnI ZraI Enzymes that do not cut: AarI Acc65I AclI AfeI AflII AflIII AleI AloI ApaI AscI AseI AsiSI AvrII BaeI BbvCI BfrBI BglII BmgBI BmtI BsaBI BsaXI BspHI BsrGI BssHII BssSI BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI EciI EcoNI EcoRI EcoRV FseI FspI FspAI HindIII HpaI KpnI MfeI MluI MmeI NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI PciI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SalI SanDI SbfI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI TaqII XbaI XhoI