(Linear) (Six-base) MAPSORT of: KIEE2440.seq Check: 2639 from: 1 to: 9225 KIEE2440.orf With 182 enzymes: SgfI * February 8, 2013 18:16 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 2422 4270 5348 8023 9138 9225 Size: 2422 1848 1078 2675 1115 87 Fragments arranged by size: 2675 2422 1848 1115 1078 87 AatII G_ACGT'C Cuts at: 0 1829 3292 7903 9225 Size: 1829 1463 4611 1322 Fragments arranged by size: 4611 1829 1463 1322 AccI GT'mk_AC Cuts at: 0 1799 3151 5594 5603 8003 8438 9019 9225 Size: 1799 1352 2443 9 2400 435 581 206 Fragments arranged by size: 2443 2400 1799 1352 581 435 206 9 Acc65I G'GTAC_C Cuts at: 0 920 4136 7726 9225 Size: 920 3216 3590 1499 Fragments arranged by size: 3590 3216 1499 920 AclI AA'CG_TT Cuts at: 0 1427 9225 Size: 1427 7798 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2632 3730 5059 5195 6023 9150 9225 Size: 2632 1098 1329 136 828 3127 75 Fragments arranged by size: 3127 2632 1329 1098 828 136 75 AfeI AGC'GCT Cuts at: 0 621 9225 Size: 621 8604 AflII C'TTAA_G Cuts at: 0 336 9225 Size: 336 8889 AflIII A'CryG_T Cuts at: 0 967 5391 5573 7620 7668 9225 Size: 967 4424 182 2047 48 1557 Fragments arranged by size: 4424 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GGTCTCn'nnnn_ Cuts at: 0 2743 3325 3674 8155 8361 9225 Size: 2743 582 349 4481 206 864 Fragments arranged by size: 4481 2743 864 582 349 206 BsaAI yAC'GTr Cuts at: 0 3495 7623 9225 Size: 3495 4128 1602 Fragments arranged by size: 4128 3495 1602 BsaBI GATnn'nnATC Cuts at: 0 4999 9225 Size: 4999 4226 BsaHI Gr'CG_yC Cuts at: 0 1644 1826 2297 2636 3289 3299 3743 4430 Size: 1644 182 471 339 653 10 444 687 Cuts at: 4430 7900 8780 9225 Size: 3470 880 445 Fragments arranged by size: 3470 1644 880 687 653 471 445 444 339 182 10 BsaWI w'CCGG_w Cuts at: 0 1785 4610 7184 7457 9225 Size: 1785 2825 2574 273 1768 Fragments arranged by size: 2825 2574 1785 1768 273 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1061 1091 2423 2453 3026 3056 3103 3133 Size: 1061 30 1332 30 573 30 47 30 Cuts at: 3133 6532 6562 8488 8518 9225 Size: 3399 30 1926 30 707 Fragments arranged by size: 3399 1926 1332 1061 707 573 47 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1082 1085 4464 6289 8757 9225 Size: 1082 3 3379 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Cuts at: 0 200 708 747 1613 9225 Size: 200 508 39 866 7612 Fragments arranged by size: 7612 866 508 200 39 Bsu36I CC'TnA_GG Cuts at: 0 3280 9225 Size: 3280 5945 BtgI C'CryG_G Cuts at: 0 838 1103 1670 2232 2835 2952 4551 6182 Size: 838 265 567 562 603 117 1599 1631 Cuts at: 6182 6882 7721 7929 8456 9108 9225 Size: 700 839 208 527 652 117 Fragments arranged by size: 1631 1599 839 838 700 652 603 567 562 527 265 208 117 117 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 3182 4631 9225 Size: 3182 1449 4594 Fragments arranged by size: 4594 3182 1449 BtsI GCAGTG_nn' Cuts at: 0 52 871 1386 2317 2797 4585 6509 7563 Size: 52 819 515 931 480 1788 1924 1054 Cuts at: 7563 7740 7903 8368 8666 8798 9225 Size: 177 163 465 298 132 427 Fragments arranged by size: 1924 1788 1054 931 819 515 480 465 427 298 177 163 132 52 ClaI AT'CG_AT Cuts at: 0 7211 9225 Size: 7211 2014 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 6864 6899 9225 Size: 6864 35 2326 Fragments arranged by size: 6864 2326 35 DraI TTT'AAA Cuts at: 0 5030 6804 9225 Size: 5030 1774 2421 Fragments arranged by size: 5030 2421 1774 DraIII CAC_nnn'GTG Cuts at: 0 1155 2992 3064 4507 6646 9225 Size: 1155 1837 72 1443 2139 2579 Fragments arranged by size: 2579 2139 1837 1443 1155 72 DrdI GACnn_nn'nnGTC Cuts at: 0 8842 9225 Size: 8842 383 EaeI y'GGCC_r Cuts at: 0 819 2105 2235 3180 3210 4970 5756 5828 Size: 819 1286 130 945 30 1760 786 72 Cuts at: 5828 6041 6185 7989 8442 9225 Size: 213 144 1804 453 783 Fragments arranged by size: 1804 1760 1286 945 819 786 783 453 213 144 130 72 30 EagI C'GGCC_G Cuts at: 0 5828 8442 9225 Size: 5828 2614 783 Fragments arranged by size: 5828 2614 783 EarI CTCTTCn'nnn_ Cuts at: 0 1889 3536 4052 5078 5433 6469 8942 9225 Size: 1889 1647 516 1026 355 1036 2473 283 Fragments arranged by size: 2473 1889 1647 1036 1026 516 355 283 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1766 9225 Size: 1766 7459 EcoICRI GAG'CTC Cuts at: 0 5424 6662 9225 Size: 5424 1238 2563 Fragments arranged by size: 5424 2563 1238 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 957 2632 3053 3331 3505 3730 4786 5059 Size: 957 1675 421 278 174 225 1056 273 Cuts at: 5059 5195 5829 6023 7468 8140 8226 8281 8773 Size: 136 634 194 1445 672 86 55 492 Cuts at: 8773 9150 9225 Size: 377 75 Fragments arranged by size: 1675 1445 1056 957 672 634 492 421 377 278 273 225 194 174 136 86 75 55 EcoNI CCTnn'n_nnAGG Cuts at: 0 2342 3313 3964 7288 7764 9225 Size: 2342 971 651 3324 476 1461 Fragments arranged by size: 3324 2342 1461 971 651 476 EcoO109I rG'GnC_Cy Cuts at: 0 1208 1405 1406 2150 3347 3500 3591 4598 Size: 1208 197 1 744 1197 153 91 1007 Cuts at: 4598 4836 6540 7114 7293 9225 Size: 238 1704 574 179 1932 Fragments arranged by size: 1932 1704 1208 1197 1007 744 574 238 197 179 153 91 1 EcoRI G'AATT_C Cuts at: 0 649 5371 7880 9225 Size: 649 4722 2509 1345 Fragments arranged by size: 4722 2509 1345 649 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 6253 9225 Size: 6253 2972 FspI TGC'GCA Cuts at: 0 2282 9091 9225 Size: 2282 6809 134 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5555 5572 9225 Size: 244 126 282 17 3653 Fragments arranged by size: 3653 2237 1212 350 282 277 261 244 204 186 176 126 17 MspA1I CmG'CkG Cuts at: 0 1672 1974 2110 2245 2555 5217 5626 8246 Size: 1672 302 136 135 310 2662 409 2620 Cuts at: 8246 8301 8610 8884 9110 9225 Size: 55 309 274 226 115 Fragments arranged by size: 2662 2620 1672 409 310 309 302 274 226 136 135 115 55 NaeI GCC'GGC Cuts at: 0 52 2845 9225 Size: 52 2793 6380 Fragments arranged by size: 6380 2793 52 NarI GG'CG_CC Cuts at: 0 1644 2636 9225 Size: 1644 992 6589 Fragments arranged by size: 6589 1644 992 NcoI C'CATG_G Cuts at: 0 838 1103 2835 2952 6182 7929 8456 9225 Size: 838 265 1732 117 3230 1747 527 769 Fragments arranged by size: 3230 1747 1732 838 769 527 265 117 NdeI CA'TA_TG Cuts at: 0 360 4761 8889 9225 Size: 360 4401 4128 336 Fragments arranged by size: 4401 4128 360 336 NgoMIV G'CCGG_C Cuts at: 0 50 2843 9225 Size: 50 2793 6382 Fragments arranged by size: 6382 2793 50 NheI G'CTAG_C Cuts at: 0 7200 9126 9225 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Size: 1208 942 1350 1336 1704 574 179 1932 Fragments arranged by size: 1932 1704 1350 1336 1208 942 574 179 PshAI GACnn'nnGTC Cuts at: 0 1351 4433 9225 Size: 1351 3082 4792 Fragments arranged by size: 4792 3082 1351 PsiI TTA'TAA Cuts at: 0 7145 9225 Size: 7145 2080 PspOMI G'GGCC_C Cuts at: 0 1405 9225 Size: 1405 7820 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 8638 8670 9225 Size: 8638 32 555 Fragments arranged by size: 8638 555 32 PstI C_TGCA'G Cuts at: 0 866 1537 2554 2860 3442 4210 4994 6173 Size: 866 671 1017 306 582 768 784 1179 Cuts at: 6173 6366 7735 9225 Size: 193 1369 1490 Fragments arranged by size: 1490 1369 1179 1017 866 784 768 671 582 306 193 PvuII CAG'CTG Cuts at: 0 1974 2245 8301 8610 8884 9225 Size: 1974 271 6056 309 274 341 Fragments arranged by size: 6056 1974 341 309 274 271 SacI G_AGCT'C Cuts at: 0 5426 6664 9225 Size: 5426 1238 2561 Fragments arranged by size: 5426 2561 1238 SacII CC_GC'GG Cuts at: 0 1673 9111 9225 Size: 1673 7438 114 Fragments arranged by size: 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812 209 709 688 4061 Fragments arranged by size: 4061 2172 812 709 688 336 238 209 SphI G_CATG'C Cuts at: 0 1822 9225 Size: 1822 7403 SspI AAT'ATT Cuts at: 0 3886 9225 Size: 3886 5339 StuI AGG'CCT Cuts at: 0 62 1441 1720 3127 6823 6848 7694 8034 Size: 62 1379 279 1407 3696 25 846 340 Cuts at: 8034 8519 9225 Size: 485 706 Fragments arranged by size: 3696 1407 1379 846 706 485 340 279 62 25 StyI C'CwwG_G Cuts at: 0 838 1103 1725 2835 2952 3233 3675 5433 Size: 838 265 622 1110 117 281 442 1758 Cuts at: 5433 6182 7318 7760 7929 8334 8456 8511 9225 Size: 749 1136 442 169 405 122 55 714 Fragments arranged by size: 1758 1136 1110 838 749 714 622 442 442 405 281 265 169 122 117 55 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 528 651 1352 3586 4064 6875 7297 7515 Size: 528 123 701 2234 478 2811 422 218 Cuts at: 7515 8575 9028 9225 Size: 1060 453 197 Fragments arranged by size: 2811 2234 1060 701 528 478 453 422 218 197 123 TatI w'GTAC_w Cuts at: 0 1697 2983 3713 3953 4300 5478 5654 9225 Size: 1697 1286 730 240 347 1178 176 3571 Fragments arranged by size: 3571 1697 1286 1178 730 347 240 176 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1428 1460 2142 2174 2423 2455 2922 2954 Size: 1428 32 682 32 249 32 467 32 Cuts at: 2954 3103 3135 6862 6894 9024 9056 9225 Size: 149 32 3727 32 2130 32 169 Fragments arranged by size: 3727 2130 1428 682 467 249 169 149 32 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 0 2593 5563 5599 9219 9225 Size: 2593 2970 36 3620 6 Fragments arranged by size: 3620 2970 2593 36 6 XbaI T'CTAG_A Cuts at: 0 5490 9225 Size: 5490 3735 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 2097 2425 2806 4398 7359 7523 8463 8490 Size: 2097 328 381 1592 2961 164 940 27 Cuts at: 8490 8860 9225 Size: 370 365 Fragments arranged by size: 2961 2097 1592 940 381 370 365 328 164 27 XmaI C'CCGG_G Cuts at: 0 1490 2460 8081 9225 Size: 1490 970 5621 1144 Fragments arranged by size: 5621 1490 1144 970 ZraI GAC'GTC Cuts at: 0 1827 3290 7901 9225 Size: 1827 1463 4611 1324 Fragments arranged by size: 4611 1827 1463 1324 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AleI AlfI AloI AlwNI ApaI ApoI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NspI PasI PciI PflMI PfoI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuII SacI SacII SalI SanDI ScaI SexAI SfcI SfoI SgrAI SmaI SmlI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI ZraI Enzymes that do not cut: AgeI AhdI ApaLI AscI AseI AsiSI BaeI BfrBI BsiWI BsrBI BssHII BstZ17I EcoRV FseI MluI NotI NruI NsiI PacI PmeI PspXI PvuI RsrII SapI SbfI SfiI SgfI SnaBI SpeI SrfI SwaI XhoI XmnI