################################## # NO CUT ENZYMES ( 131 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AfaI AflII AflIII AgeI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaHI BseAI BsgI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstYI BstZ17I CelII Cfr10I ClaI CpoI DraIII DrdI EaeI EagI EclXI Eco47III Eco52I EcoRV EcoT22I FseI FspI HaeII HgaI Hin1I HindIII HpaI KasI KpnI MaeII MfeI MflI MluI MluNI MroI MscI MunI NaeI NarI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshBI Psp1406I PstI PvuI PvuII RsaI RsrII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI XbaI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AccI 735 GTCTAC GT^[AC][GT]AC AccII 616 CGCG CG^CG AhdI 349 GACCAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwI 598 GGATC GGATC(4/5) AocI 1166 CCTCAGG CC^T[ACGT]AGG Asp700I 1115 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 349 GACCAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 170 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC BanII 225 GAGCTC G[GA]GC[CT]^C BglI 1580 GCCGGGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpmI 159 CTCCAG CTGGAG(16/14) BsaBI 367 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 796 ACCGGA [AT]^CCGG[AT] BspHI 971 TCATGA T^CATGA BsrDI 1136 GCAATG GCAATG(2/0) BstAPI 1127 GCAATCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 610 GGTGACC G^GT[ACGT]ACC BstF5I 576 GGATG GGATG(2/0) BstPI 610 GGTGACC G^GT[ACGT]ACC BstUI 616 CGCG CG^CG BstXI 75 CCAAGGCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 1166 CCTCAGG CC^T[ACGT]AGG Cac8I 236 GCCAGC GC[ACGT]^[ACGT]GC CfoI 1761 GCGC GCG^C DraI 760 TTTAAA TTT^AAA DraII 585 AGGTCCT [GA]G^G[ACGT]CC[CT] Eam1105I 349 GACCAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 1166 CCTCAGG CC^T[ACGT]AGG EcoNI 589 CCTATGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 585 AGGTCCT [GA]G^G[ACGT]CC[CT] EcoO65I 610 GGTGACC G^GT[ACGT]ACC FauI 533 GCGGG CCCGC(4/6) FokI 576 GGATG GGATG(9/13) HaeIII 1006 GGCC GG^CC HhaI 1761 GCGC GCG^C HinP1I 1761 GCGC G^CGC HincII 332 GTCAAC GT[CT]^[GA]AC HindII 332 GTCAAC GT[CT]^[GA]AC KspI 615 CCGCGG CCGC^GG MamI 367 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MslI 936 CATTAAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 616 CGCG CG^CG NcoI 924 CCATGG C^CATGG NlaIV 655 GGACCC GG[ACGT]^[ACGT]CC PflFI 170 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 1156 GACTC GAGTC(4/5) PpuMI 585 AGGTCCT [GA]G^G[AT]CC[CT] RcaI 971 TCATGA T^CATGA SacI 225 GAGCTC GAGCT^C SacII 615 CCGCGG CCGC^GG SapI 299 GAAGAGC GCTCTTC(1/4) Tth111I 170 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 318 CCAGCCAGACTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 1115 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AlwNI 665 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG 677 CAGAATCTG AspHI 225 GAGCTC G[AT]GC[AT]^C 302 GAGCAC AvaII 586 GGTCC G^G[AT]CC 655 GGACC BbsI 792 GAAGAC GAAGAC(2/6) 959 GAAGAC BcnI 1182 CCGGG CC^[GC]GG 1581 CCGGG BfaI 956 CTAG C^TAG 1145 CTAG BmyI 225 GAGCTC G[AGT]GC[ACT]^C 302 GAGCAC BpuAI 792 GAAGAC GAAGAC(2/6) 959 GAAGAC BsiHKAI 225 GAGCTC G[AT]GC[AT]^C 302 GAGCAC Bsp1286I 225 GAGCTC G[AGT]GC[ACT]^C 302 GAGCAC BsrI 902 ACTGG ACTGG(1/-1) 161 CCAGT BstNI 67 CCAGG CC^[AT]GG 255 CCAGG Cfr13I 586 GGTCC G^G[ACGT]CC 655 GGACC DdeI 887 CTAAG C^T[ACGT]AG 1167 CTCAG EarI 299 GAAGAG CTCTTC(1/4) 581 GAAGAG EcoRII 67 CCAGG ^CC[AT]GG 255 CCAGG HphI 34 GGTGA GGTGA(8/7) 610 GGTGA Ksp632I 299 GAAGAG CTCTTC(1/4) 581 GAAGAG MaeI 956 CTAG C^TAG 1145 CTAG MseI 761 TTAA T^TAA 938 TTAA MvaI 67 CCAGG CC^[AT]GG 255 CCAGG NciI 1182 CCGGG CC^[GC]GG 1581 CCGGG NdeI 344 CATATG CA^TATG 383 CATATG PflMI 322 CCAGACTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 896 CCATTGACTGG PshAI 325 GACTATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 1156 GACTCTCGTC Sau96I 586 GGTCC G^G[ACGT]CC 655 GGACC SfaNI 667 GATGC GCATC(5/9) 91 GCATC Tru9I 761 TTAA T^TAA 938 TTAA Van91I 322 CCAGACTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 896 CCATTGACTGG ######################################### # 3 OR MORE CUT ENZYMES ( 44 enzymes) # ######################################### AciI AcsI AluI ApoI BbvI BclI BsaI BsaJI BseRI BsiYI BslI BsmAI BsmFI BssKI DpnII DsaI EcoRI EcoT14I FbaI Fnu4HI HapII HinfI HpaII ItaI MaeIII MboI MboII MnlI MspA1I MspI MwoI NdeII NlaIII Sau3AI ScrFI SfcI StyI TaqI TfiI TseI Tsp45I Tsp509I TspRI TthHB8I