Restriction map for pFN21AE3496
 (Circular) (Six-base) MAPSORT of: pFN21AE3496.seq  Check: 6718  from: 1  to: 10615



pFN21AE3496  10615 bp

 With 182 enzymes: SgfI * 

                        July 19, 2019 13:42  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   3330    3525    5145    6805    3330 
   Size:        195    1620    1660    7140

  Fragments arranged by size: 

                7140    1660    1620     195
 
AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3663    8425     278 
   Size:         53      83     186     936    2127    4762    2468

  Fragments arranged by size: 

                4762    2468    2127     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2436    3443    3707    5095    5589    7562    7707    7958 
   Size:        499    1007     264    1388     494    1973     145     251

Cuts at:   7958    1937 
   Size:       4594

  Fragments arranged by size: 

                4594    1973    1388    1007     499     494     264     251
                 145
 
Acc65I G'GTAC_C

Cuts at:   2562    7936    2562 
   Size:       5374    5241
 
AclI AA'CG_TT

Cuts at:   1563    5498    7914    8742    9115    1563 
   Size:       3935    2416     828     373    3063

  Fragments arranged by size: 

                3935    3063    2416     828     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2117    3041    6658    8678   10062   10330    1632 
   Size:        485     924    3617    2020    1384     268    1917

  Fragments arranged by size: 

                3617    2020    1917    1384     924     485     268
 
AflII C'TTAA_G

Cuts at:    820    1017    4730    5375     820 
   Size:        197    3713     645    6060

  Fragments arranged by size: 

                6060    3713     645     197
 
AflIII A'CryG_T

Cuts at:   1203    2222    2431    3351    9514   10491    1203 
   Size:       1019     209     920    6163     977    1327

  Fragments arranged by size: 

                6163    1327    1019     977     920     209
 
AgeI A'CCGG_T

Cuts at:   9446    9446 
   Size:      10615
 
AhdI GACnn_n'nnGTC

Cuts at:   2244    6257    9344    2244 
   Size:       4013    3087    3515

  Fragments arranged by size: 

                4013    3515    3087
 
AleI CACnn'nnGTG

Cuts at:   1166    2013    2954    3354    3635    3884    5558    1166 
   Size:        847     941     400     281     249    1674    6223

  Fragments arranged by size: 

                6223    1674     941     847     400     281     249
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    2826    3305    6457    1184 
   Size:       1642     479    3152    5342

  Fragments arranged by size: 

                5342    3152    1642     479
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2477    2509    2566    2598    2477 
   Size:         32      57      32   10494

  Fragments arranged by size: 

               10494      57      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:   3529    3556    4720    8358    9930    3529 
   Size:         27    1164    3638    1572    4214

  Fragments arranged by size: 

                4214    3638    1572    1164      27
 
ApaI G_GGCC'C

Cuts at:   4792    4792 
   Size:      10615
 
ApaLI G'TGCA_C

Cuts at:   8672    9828    8672 
   Size:       1156    9459
 
ApoI r'AATT_y

Cuts at:   1493    3644    4868    5984    7924    8150    8302   10411    1493 
   Size:       2151    1224    1116    1940     226     152    2109    1697

  Fragments arranged by size: 

                2151    2109    1940    1697    1224    1116     226     152
            
 
AseI AT'TA_AT

Cuts at:    160    1032    9169     160 
   Size:        872    8137    1606

  Fragments arranged by size: 

                8137    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:      10615
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2026    2182    4248    5252    5448    6718    7940 
   Size:         73      83     156    2066    1004     196    1270    1222

Cuts at:   7940    1870 
   Size:       4545

  Fragments arranged by size: 

                4545    2066    1270    1222    1004     196     156      83
                  73
 
BamHI G'GATC_C

Cuts at:   2574    6006    7945    8312    2574 
   Size:       3432    1939     367    4877

  Fragments arranged by size: 

                4877    3432    1939     367
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2562    3248    6590    6764    7936 
   Size:        325     217     627     775     686    3342     174    1172

Cuts at:   7936    9391     618 
   Size:       1455    1842

  Fragments arranged by size: 

                3342    1842    1455    1172     775     686     627     325
                 217     174
 
BanII G_rGCy'C

Cuts at:   1391    2724    2810    4185    4545    4792    5272    5385    6716 
   Size:       1333      86    1375     360     247     480     113    1331

Cuts at:   6716    6810    7934    1391 
   Size:         94    1124    4072

  Fragments arranged by size: 

                4072    1375    1333    1331    1124     480     360     247
                 113      94      86
 
BbeI G_GCGC'C

Cuts at:   3252    6594    3252 
   Size:       3342    7273
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2315    3655    3849    4689    5419    5845     928 
   Size:        971     416    1340     194     840     730     426    5698

  Fragments arranged by size: 

                5698    1340     971     840     730     426     416     194
            
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    1987    2021    8806    8840    1903 
   Size:         34      50      34    6785      34    3678

  Fragments arranged by size: 

                6785    3678      50      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3821    4571    8507    9717    3821 
   Size:        750    3936    1210    4719

  Fragments arranged by size: 

                4719    3936    1210     750
 
BclI T'GATC_A

Cuts at:   1548    3909    4596    1548 
   Size:       2361     687    7567

  Fragments arranged by size: 

                7567    2361     687
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2259    2550    4695 
   Size:        107     122      71     631     605     587     291    2145

Cuts at:   4695    9226     136 
   Size:       4531    1525

  Fragments arranged by size: 

                4531    2145    1525     631     605     587     291     122
                 107      71
 
BglII A'GATC_T

Cuts at:   2673    6096   10610    2673 
   Size:       3423    4514    2678

  Fragments arranged by size: 

                4514    3423    2678
 
BlpI GC'TnA_GC

Cuts at:   4832    8032    4832 
   Size:       3200    7415
 
Bme1580I G_kGCm'C

Cuts at:   1790    2772    3147    3944    4792    5370    8676    9832    1790 
   Size:        982     375     797     848     578    3306    1156    2573

  Fragments arranged by size: 

                3306    2573    1156     982     848     797     578     375
            
 
BmgBI CAC'GTC

Cuts at:   1318    3711    3945    4828    6058    6078    6157    6316    1318 
   Size:       2393     234     883    1230      20      79     159    5617

  Fragments arranged by size: 

                5617    2393    1230     883     234     159      79      20
            
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3303    6519    6597 
   Size:        416      53      83     399      24    1886    3216      78

Cuts at:   6597    6642    8346    9304     442 
   Size:         45    1704     958    1753

  Fragments arranged by size: 

                3216    1886    1753    1704     958     416     399      83
                  78      53      45      24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:      10615
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   6829    6829 
   Size:      10615
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2918    3551    3689    4199    4941    5507    6032    6158 
   Size:       1679     633     138     510     742     566     525     126

Cuts at:   6158    7686    9275    1239 
   Size:       1528    1589    2579

  Fragments arranged by size: 

                2579    1679    1589    1528     742     633     566     525
                 510     138     126
 
Bpu10I CC'TnA_GC

Cuts at:   4901    4901 
   Size:      10615
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2996    6721    8740    9605    9903    2996 
   Size:       3725    2019     865     298    3708

  Fragments arranged by size: 

                3725    3708    2019     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    6370    6705    9278     882 
   Size:        617    4871     335    2573    2219

  Fragments arranged by size: 

                4871    2573    2219     617     335
 
BsaAI yAC'GTr

Cuts at:    493    1204    2293    8406     493 
   Size:        711    1089    6113    2702

  Fragments arranged by size: 

                6113    2702    1089     711
 
BsaBI GATnn'nnATC

Cuts at:   4343    5966    6242   10609    4343 
   Size:       1623     276    4367    4349

  Fragments arranged by size: 

                4367    4349    1623     276
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3004    3249    3660    6591 
   Size:         53      83     186     936    1471     245     411    2931

Cuts at:   6591    8422    8804    9439     275 
   Size:       1831     382     635    1451

  Fragments arranged by size: 

                2931    1831    1471    1451     936     635     411     382
                 245     186      83      53
 
BsaWI w'CCGG_w

Cuts at:   2086    4319    4416    5673    9048    9446    9720    9867   10278 
   Size:       2233      97    1257    3375     398     274     147     411

Cuts at:  10278    2086 
   Size:       2423

  Fragments arranged by size: 

                3375    2423    2233    1257     411     398     274     147
                  97
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    5207    5237    6146    6176    7810 
   Size:         30     165      30    3804      30     909      30    1634

Cuts at:   7810    7840    1178 
   Size:         30    3953

  Fragments arranged by size: 

                3953    3804    1634     909     165      30      30      30
                  30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2492    2711    2921    4034    4255    5186    5831    7922 
   Size:       1306     219     210    1113     221     931     645    2091

Cuts at:   7922    1186 
   Size:       3879

  Fragments arranged by size: 

                3879    2091    1306    1113     931     645     221     219
                 210
 
BseYI C'CCAG_C

Cuts at:   2710    5359    6109    6382    6776    6926    6947    6968    6989 
   Size:       2649     750     273     394     150      21      21      21

Cuts at:   6989    7010    7031    7094    7115    7136    7157    7199    7283 
   Size:         21      21      63      21      21      21      42      84

Cuts at:   7283    7304    7388    7472    7484    7493    7547    9818   10314 
   Size:         21      84      84      12       9      54    2271     496

Cuts at:  10314    2710 
   Size:       3011

  Fragments arranged by size: 

                3011    2649    2271     750     496     394     273     150
                  84      84      84      63      54      42      21      21
                  21      21      21      21      21      21      21      12
                   9
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2247    3380    3638    4685    5000    1163 
   Size:        573     511    1133     258    1047     315    6778

  Fragments arranged by size: 

                6778    1133    1047     573     511     315     258
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    6402    8065    8826    9854    1540 
   Size:        275     186    4401    1663     761    1028    2301

  Fragments arranged by size: 

                4401    2301    1663    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   4902    5385    5555    5775    8676    8761    9832    4902 
   Size:        483     170     220    2901      85    1071    5685

  Fragments arranged by size: 

                5685    2901    1071     483     220     170      85
 
BsiWI C'GTAC_G

Cuts at:   6067    6067 
   Size:      10615
 
BsmI GAATG_Cn'

Cuts at:   3429    4233    4975    8126    8219    3429 
   Size:        804     742    3151      93    5825

  Fragments arranged by size: 

                5825    3151     804     742      93
 
BsmBI CGTCTCn'nnnn_

Cuts at:   2132    3527    3651    6008    7095    2132 
   Size:       1395     124    2357    1087    5652

  Fragments arranged by size: 

                5652    2357    1395    1087     124
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2724    2772    2810    3147    3944    4185    4545 
   Size:        399     934      48      38     337     797     241     360

Cuts at:   4545    4792    4902    5272    5370    5385    5555    5775    6716 
   Size:        247     110     370      98      15     170     220     941

Cuts at:   6716    6810    7934    8676    8761    9832    1391 
   Size:         94    1124     742      85    1071    2174

  Fragments arranged by size: 

                2174    1124    1071     941     934     797     742     399
                 370     360     337     247     241     220     170     110
                  98      94      85      48      38      15
 
BspEI T'CCGG_A

Cuts at:   2086    4319   10278    2086 
   Size:       2233    5959    2423

  Fragments arranged by size: 

                5959    2423    2233
 
BspHI T'CATG_A

Cuts at:   3988    5787    6285    8504   10234    3988 
   Size:       1799     498    2219    1730    4369

  Fragments arranged by size: 

                4369    2219    1799    1730     498
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    3330    3525    4959    5022    5145    5305    6805    7970 
   Size:       2486     195    1434      63     123     160    1500    1165

Cuts at:   7970     844 
   Size:       3489

  Fragments arranged by size: 

                3489    2486    1500    1434    1165     195     160     123
                  63
 
BsrBI CCG'CTC

Cuts at:   1394    1823    2203    2776    3150    3673    4864    4876    5447 
   Size:        429     380     573     374     523    1191      12     571

Cuts at:   5447    6142    6813    8502    1394 
   Size:        695     671    1689    3507

  Fragments arranged by size: 

                3507    1689    1191     695     671     573     571     523
                 429     380     374      12
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2887    2910    3153    4080    4356    5241    9110 
   Size:       1183    1639      23     243     927     276     885    3869

Cuts at:   9110    9284      65 
   Size:        174    1396

  Fragments arranged by size: 

                3869    1639    1396    1183     927     885     276     243
                 174      23
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    3329    3381    3733    4490    6401    6500    6635 
   Size:        110    1519      52     352     757    1911      99     135

Cuts at:   6635    9259    9446    1700 
   Size:       2624     187    2869

  Fragments arranged by size: 

                2869    2624    1911    1519     757     352     187     135
                 110      99      52
 
BsrGI T'GTAC_A

Cuts at:     96    5925      96 
   Size:       5829    4786
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:      10615
 
BssSI C'ACGA_G

Cuts at:   8675    9687    8675 
   Size:       1012    9603
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    6411    6581     794 
   Size:       5617     170    4828

  Fragments arranged by size: 

                5617    4828     170
 
BstBI TT'CG_AA

Cuts at:   9419    9419 
   Size:      10615
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:      10615
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2956    3889    5029    5333    6532    2956 
   Size:        933    1140     304    1199    7039

  Fragments arranged by size: 

                7039    1199    1140     933     304
 
BstYI r'GATC_y

Cuts at:   1972    2524    2574    2643    2673    3546    6006    6096    6459 
   Size:        552      50      69      30     873    2460      90     363

Cuts at:   6459    7945    8312    8697    8714   10166   10252   10264   10610 
   Size:       1486     367     385      17    1452      86      12     346

Cuts at:  10610    1972 
   Size:       1977

  Fragments arranged by size: 

                2460    1977    1486    1452     873     552     385     367
                 363     346      90      86      69      50      30      17
                  12
 
BstZ17I GTA'TAC

Cuts at:   7563    7563 
   Size:      10615
 
Bsu36I CC'TnA_GG

Cuts at:   2511    2511 
   Size:      10615
 
BtgI C'CryG_G

Cuts at:    513    1065    2545    2952    3437    3561    4462    4524    4605 
   Size:        552    1480     407     485     124     901      62      81

Cuts at:   4605    5685    6531     513 
   Size:       1080     846    4597

  Fragments arranged by size: 

                4597    1480    1080     901     846     552     485     407
                 124      81      62
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226    5811     487 
   Size:        683      56    4585    5291

  Fragments arranged by size: 

                5291    4585     683      56
 
BtsI GCAGTG_nn'

Cuts at:   3642    8131    8925    8945    3642 
   Size:       4489     794      20    5312

  Fragments arranged by size: 

                5312    4489     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:      10615
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35   10580
 
DraI TTT'AAA

Cuts at:   7919    7919 
   Size:      10615
 
DraIII CAC_nnn'GTG

Cuts at:   5151    6341    6761    5151 
   Size:       1190     420    9005

  Fragments arranged by size: 

                9005    1190     420
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4556    4931    9622     809 
   Size:        507    3240     375    4691    1802

  Fragments arranged by size: 

                4691    3240    1802     507     375
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2209    2251    2260 
   Size:         54    1425      50     275      18     379      42       9

Cuts at:   2260    2274    2735    2946    3558    3735    6229    6240    6399 
   Size:         14     461     211     612     177    2494      11     159

Cuts at:   6399    8062    8951   10580       8 
   Size:       1663     889    1629      43

  Fragments arranged by size: 

                2494    1663    1629    1425     889     612     461     379
                 275     211     177     159      54      50      43      42
                  18      14      11       9
 
EagI C'GGCC_G

Cuts at:   1537    1812    6399    8062    1537 
   Size:        275    4587    1663    4090

  Fragments arranged by size: 

                4587    4090    1663     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    4010    5129    5817    8545    1353 
   Size:       2657    1119     688    2728    3423

  Fragments arranged by size: 

                3423    2728    2657    1119     688
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    9202    9576    9722     387 
   Size:       1064     138    7613     374     146    1280

  Fragments arranged by size: 

                7613    1280    1064     374     146     138
 
EcoICRI GAG'CTC

Cuts at:   5383    5383 
   Size:      10615
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2117    2918    3041    3551    3689    4199    4941 
   Size:        393     485     801     123     510     138     510     742

Cuts at:   4941    5507    6032    6158    6658    7686    8678    9275   10062 
   Size:        566     525     126     500    1028     992     597     787

Cuts at:  10062   10330    1239 
   Size:        268    1524

  Fragments arranged by size: 

                1524    1028     992     801     787     742     597     566
                 525     510     510     500     485     393     268     138
                 126     123
 
EcoNI CCTnn'n_nnAGG

Cuts at:   2447    4049    4820    6756    2447 
   Size:       1602     771    1936    6306

  Fragments arranged by size: 

                6306    1936    1602     771
 
EcoO109I rG'GnC_Cy

Cuts at:   3724    4788    4789    4813    5992    6133    6723    6731    3724 
   Size:       1064       1      24    1179     141     590       8    7608

  Fragments arranged by size: 

                7608    1179    1064     590     141      24       8       1
            
 
EcoRI G'AATT_C

Cuts at:   5984    7924    8302    5984 
   Size:       1940     378    8297

  Fragments arranged by size: 

                8297    1940     378
 
EcoRV GAT'ATC

Cuts at:   2389    2389 
   Size:      10615
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   3793    4783    6313    3793 
   Size:        990    1530    8095

  Fragments arranged by size: 

                8095    1530     990
 
FspI TGC'GCA

Cuts at:   2051    3224    3641    5247    5280    9121    2051 
   Size:       1173     417    1606      33    3841    3545

  Fragments arranged by size: 

                3841    3545    1606    1173     417      33
 
HaeII r_GCGC'y

Cuts at:   1678    3252    6594    9762    1678 
   Size:       1574    3342    3168    2531

  Fragments arranged by size: 

                3342    3168    2531    1574
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2230    2262    2851    2883    3403 
   Size:         32     135      32     658      32     589      32     520

Cuts at:   3403    3435    3531    3563    4411    4443    5207    5239    6243 
   Size:         32      96      32     848      32     764      32    1004

Cuts at:   6243    6275    9256    9288    9330    9362    1373 
   Size:         32    2981      32      42      32    2626

  Fragments arranged by size: 

                2981    2626    1004     848     764     658     589     520
                 135      96      42      32      32      32      32      32
                  32      32      32      32      32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    3708    5096    6262    7959    8205     669 
   Size:        988     281    1770    1388    1166    1697     246    3079

  Fragments arranged by size: 

                3079    1770    1697    1388    1166     988     281     246
            
 
HindIII A'AGCT_T

Cuts at:    748    2162     748 
   Size:       1414    9201
 
HpaI GTT'AAC

Cuts at:   8205    8205 
   Size:      10615
 
KasI G'GCGC_C

Cuts at:   3248    6590    3248 
   Size:       3342    7273
 
KpnI G_GTAC'C

Cuts at:   2566    7940    2566 
   Size:       5374    5241
 
MfeI C'AATT_G

Cuts at:   3805    8214    3805 
   Size:       4409    6206
 
MluI A'CGCG_T

Cuts at:  10491   10491 
   Size:      10615
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4038    4348    4764    4839    5034    5898    6920    7277 
   Size:       2733     310     416      75     195     864    1022     357

Cuts at:   7277    7403    7475    9729    9913    1305 
   Size:        126      72    2254     184    2007

  Fragments arranged by size: 

                2733    2254    2007    1022     864     416     357     310
                 195     184     126      75      72
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2253    2262    2276    2948    3560 
   Size:         54    1425     343     421       9      14     672     612

Cuts at:   3560    6231    6242      10 
   Size:       2671      11    4383

  Fragments arranged by size: 

                4383    2671    1425     672     612     421     343      54
                  14      11       9
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2013    2619    2954    3354    3635    3834    3884 
   Size:        648     847     606     335     400     281     199      50

Cuts at:   3884    4170    5027    5306    5558    6503    8573    8932    9091 
   Size:        286     857     279     252     945    2070     359     159

Cuts at:   9091     518 
   Size:       2042

  Fragments arranged by size: 

                2070    2042     945     857     847     648     606     400
                 359     335     286     281     279     252     199     159
                  50
 
MspA1I CmG'CkG

Cuts at:   1606    2047    2214    2872    2900    3028    3950    4588    4669 
   Size:        441     167     658      28     128     922     638      81

Cuts at:   4669    6391    6669    8031    8708    9856   10101    1606 
   Size:       1722     278    1362     677    1148     245    2120

  Fragments arranged by size: 

                2120    1722    1362    1148     922     677     658     638
                 441     278     245     167     128      81      28
 
NaeI GCC'GGC

Cuts at:   3735    4492    6403    6637    3735 
   Size:        757    1911     234    7713

  Fragments arranged by size: 

                7713    1911     757     234
 
NarI GG'CG_CC

Cuts at:   3249    6591    3249 
   Size:       3342    7273
 
NcoI C'CATG_G

Cuts at:    513    1065    2545    2952    3437    3561    4524    4605    5685 
   Size:        552    1480     407     485     124     963      81    1080

Cuts at:   5685    6531     513 
   Size:        846    4597

  Fragments arranged by size: 

                4597    1480    1080     963     846     552     485     407
                 124      81
 
NdeI CA'TA_TG

Cuts at:    387    2462     387 
   Size:       2075    8540
 
NgoMIV G'CCGG_C

Cuts at:   3733    4490    6401    6635    3733 
   Size:        757    1911     234    7713

  Fragments arranged by size: 

                7713    1911     757     234
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:      10615
 
NotI GC'GGCC_GC

Cuts at:   8062    8062 
   Size:      10615
 
NspI r_CATG'y

Cuts at:   1132    1229    2043    2226    2435    3355    5881    5932    6508 
   Size:         97     814     183     209     920    2526      51     576

Cuts at:   6508    7973    9518    1132 
   Size:       1465    1545    2229

  Fragments arranged by size: 

                2526    2229    1545    1465     920     814     576     209
                 183      97      51
 
PasI CC'CwG_GG

Cuts at:   1348    4190    6626    1348 
   Size:       2842    2436    5337

  Fragments arranged by size: 

                5337    2842    2436
 
PciI A'CATG_T

Cuts at:   2222    2431    3351    9514    2222 
   Size:        209     920    6163    3323

  Fragments arranged by size: 

                6163    3323     920     209
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    3556    4525    4720    6238    1108 
   Size:       2448     969     195    1518    5485

  Fragments arranged by size: 

                5485    2448    1518     969     195
 
PfoI T'CCnGG_A

Cuts at:   3549    3666    6009    3549 
   Size:        117    2343    8155

  Fragments arranged by size: 

                8155    2343     117
 
PmeI GTTT'AAAC

Cuts at:   7919    7919 
   Size:      10615
 
PmlI CAC'GTG

Cuts at:   2293    2293 
   Size:      10615
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4745    4777    5213    5245    8684    8716   10197   10229    4745 
   Size:         32     436      32    3439      32    1481      32    5131

  Fragments arranged by size: 

                5131    3439    1481     436      32      32      32      32
            
 
PpuMI rG'GwC_Cy

Cuts at:   3724    4813    6723    6731    3724 
   Size:       1089    1910       8    7608

  Fragments arranged by size: 

                7608    1910    1089       8
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:      10615
 
PsiI TTA'TAA

Cuts at:   8185    8185 
   Size:      10615
 
PspOMI G'GGCC_C

Cuts at:   4788    4788 
   Size:      10615
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:   3224    3256    3224 
   Size:         32   10583
 
PstI C_TGCA'G

Cuts at:    830    3327    4809    5901    6000    7967     830 
   Size:       2497    1482    1092      99    1967    3478

  Fragments arranged by size: 

                3478    2497    1967    1482    1092      99
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:      10615
 
PvuII CAG'CTG

Cuts at:   2872    3950    4669    6391    2872 
   Size:       1078     719    1722    7096

  Fragments arranged by size: 

                7096    1722    1078     719
 
SacI G_AGCT'C

Cuts at:   5385    5385 
   Size:      10615
 
SalI G'TCGA_C

Cuts at:   1936    3706    5094    7957    1936 
   Size:       1770    1388    2863    4594

  Fragments arranged by size: 

                4594    2863    1770    1388
 
SanDI GG'GwC_CC

Cuts at:   6723    6723 
   Size:      10615
 
SapI GCTCTTCn'nnn_

Cuts at:   5129    5129 
   Size:      10615
 
SbfI CC_TGCA'GG

Cuts at:   5901    6000    7967    5901 
   Size:         99    1967    8549

  Fragments arranged by size: 

                8549    1967      99
 
ScaI AGT'ACT

Cuts at:   7583    7625    7730    7772    8863   10575    7583 
   Size:         42     105      42    1091    1712    7623

  Fragments arranged by size: 

                7623    1712    1091     105      42      42
 
SexAI A'CCwGG_T

Cuts at:   3132    3793    4656    4815    6757    3132 
   Size:        661     863     159    1942    6990

  Fragments arranged by size: 

                6990    1942     863     661     159
 
SfcI C'TryA_G

Cuts at:    826    1045    3323    4805    5897    5996    6910    7288    7351 
   Size:        219    2278    1482    1092      99     914     378      63

Cuts at:   7351    7477    7498    7864    7963    9098    9779    9970     826 
   Size:        126      21     366      99    1135     681     191    1471

  Fragments arranged by size: 

                2278    1482    1471    1135    1092     914     681     378
                 366     219     191     126      99      99      63      21
            
 
SfiI GGCCn_nnn'nGGCC

Cuts at:   2259    2259 
   Size:      10615
 
SfoI GGC'GCC

Cuts at:   3250    6592    3250 
   Size:       3342    7273
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:      10615
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:      10615
 
SmaI CCC'GGG

Cuts at:   1872    5254    6720    7942    1872 
   Size:       3382    1466    1222    4545

  Fragments arranged by size: 

                4545    3382    1466    1222
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3011    4730    5375    5448    6736    8719 
   Size:        197     926    1068    1719     645      73    1288    1983

Cuts at:   8719    9620    9882     820 
   Size:        901     262    1553

  Fragments arranged by size: 

                1983    1719    1553    1288    1068     926     901     645
                 262     197      73
 
SnaBI TAC'GTA

Cuts at:    493    8406     493 
   Size:       7913    2702
 
SpeI A'CTAG_T

Cuts at:    152    3217    7319     152 
   Size:       3065    4102    3448

  Fragments arranged by size: 

                4102    3448    3065
 
SphI G_CATG'C

Cuts at:   1132    2043    7973    1132 
   Size:        911    5930    3774

  Fragments arranged by size: 

                5930    3774     911
 
SspI AAT'ATT

Cuts at:      5      52    7604    8539       5 
   Size:         47    7552     935    2081

  Fragments arranged by size: 

                7552    2081     935      47
 
StuI AGG'CCT

Cuts at:   1517    5195    1517 
   Size:       3678    6937
 
StyI C'CwwG_G

Cuts at:    513    1065    2403    2545    2952    3437    3561    3882    4524 
   Size:        552    1338     142     407     485     124     321     642

Cuts at:   4524    4605    5287    5466    5685    6531    6560    8054     513 
   Size:         81     682     179     219     846      29    1494    3074

  Fragments arranged by size: 

                3074    1494    1338     846     682     642     552     485
                 407     321     219     179     142     124      81      29
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3842    4516    5318    5351    5569    6524    6538 
   Size:       1255    2262     674     802      33     218     955      14

Cuts at:   6538    6940    6961    6994    7057    7150    7204    7225    7246 
   Size:        402      21      33      63      93      54      21      21

Cuts at:   7246    7297    7318    7402    7486    7528    7624    7666    8642 
   Size:         51      21      84      84      42      96      42     976

Cuts at:   8642    8659    8812    8997    9442     325 
   Size:         17     153     185     445    1498

  Fragments arranged by size: 

                2262    1498    1255     976     955     802     674     445
                 402     218     185     153      96      93      84      84
                  63      54      51      42      42      33      33      21
                  21      21      21      17      14
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3255    5925    7518 
   Size:        274      80      33      51    1443    1278    2670    1593

Cuts at:   7518    7581    7623    7728    7770    8861   10573      96 
   Size:         63      42     105      42    1091    1712     138

  Fragments arranged by size: 

                2670    1712    1593    1443    1278    1091     274     138
                 105      80      63      51      42      42      33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3389    3421    5126    5158    5462    5494    6146    6178    6556 
   Size:         32    1705      32     304      32     652      32     378

Cuts at:   6556    6588   10575   10607    3389 
   Size:         32    3987      32    3397

  Fragments arranged by size: 

                3987    3397    1705     652     378     304      32      32
                  32      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:      10615
 
XbaI T'CTAG_A

Cuts at:   7951    7951 
   Size:      10615
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2356    2552    3889    4657    5029    6088    2356 
   Size:        196    1337     768     372    1059    6883

  Fragments arranged by size: 

                6883    1337    1059     768     372     196
 
XhoI C'TCGA_G

Cuts at:   1943    5448    1943 
   Size:       3505    7110
 
XmaI C'CCGG_G

Cuts at:   1870    5252    6718    7940    1870 
   Size:       3382    1466    1222    4545

  Fragments arranged by size: 

                4545    3382    1466    1222
 
XmnI GAAnn'nnTTC

Cuts at:   4760    4982    5321    8744    4760 
   Size:        222     339    3423    6631

  Fragments arranged by size: 

                6631    3423     339     222
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3661    8423     276 
   Size:         53      83     186     936    2127    4762    2468

  Fragments arranged by size: 

                4762    2468    2127     936     186      83      53
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI
     BbsI     BcgI    BciVI     BclI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FspI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NspI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI     PsrI     PstI     PvuI    PvuII     SacI     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SspI
     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AfeI     AscI    AvrII     BaeI    BbvCI    BfrBI     FseI    FspAI
     NruI     NsiI     PacI    PspXI    RsrII    SacII     SrfI     SwaI
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