(Circular) (Six-base) MAPSORT of: pFN21AE3496.seq Check: 6718 from: 1 to: 10615 pFN21AE3496 10615 bp With 182 enzymes: SgfI * July 19, 2019 13:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3330 3525 5145 6805 3330 Size: 195 1620 1660 7140 Fragments arranged by size: 7140 1660 1620 195 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3663 8425 278 Size: 53 83 186 936 2127 4762 2468 Fragments arranged by size: 4762 2468 2127 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2436 3443 3707 5095 5589 7562 7707 7958 Size: 499 1007 264 1388 494 1973 145 251 Cuts at: 7958 1937 Size: 4594 Fragments arranged by size: 4594 1973 1388 1007 499 494 264 251 145 Acc65I G'GTAC_C Cuts at: 2562 7936 2562 Size: 5374 5241 AclI AA'CG_TT Cuts at: 1563 5498 7914 8742 9115 1563 Size: 3935 2416 828 373 3063 Fragments arranged by size: 3935 3063 2416 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2117 3041 6658 8678 10062 10330 1632 Size: 485 924 3617 2020 1384 268 1917 Fragments arranged by size: 3617 2020 1917 1384 924 485 268 AflII C'TTAA_G Cuts at: 820 1017 4730 5375 820 Size: 197 3713 645 6060 Fragments arranged by size: 6060 3713 645 197 AflIII A'CryG_T Cuts at: 1203 2222 2431 3351 9514 10491 1203 Size: 1019 209 920 6163 977 1327 Fragments arranged by size: 6163 1327 1019 977 920 209 AgeI A'CCGG_T Cuts at: 9446 9446 Size: 10615 AhdI GACnn_n'nnGTC Cuts at: 2244 6257 9344 2244 Size: 4013 3087 3515 Fragments arranged by size: 4013 3515 3087 AleI CACnn'nnGTG Cuts at: 1166 2013 2954 3354 3635 3884 5558 1166 Size: 847 941 400 281 249 1674 6223 Fragments arranged by size: 6223 1674 941 847 400 281 249 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2826 3305 6457 1184 Size: 1642 479 3152 5342 Fragments arranged by size: 5342 3152 1642 479 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2477 2509 2566 2598 2477 Size: 32 57 32 10494 Fragments arranged by size: 10494 57 32 32 AlwNI CAG_nnn'CTG Cuts at: 3529 3556 4720 8358 9930 3529 Size: 27 1164 3638 1572 4214 Fragments arranged by size: 4214 3638 1572 1164 27 ApaI G_GGCC'C Cuts at: 4792 4792 Size: 10615 ApaLI G'TGCA_C Cuts at: 8672 9828 8672 Size: 1156 9459 ApoI r'AATT_y Cuts at: 1493 3644 4868 5984 7924 8150 8302 10411 1493 Size: 2151 1224 1116 1940 226 152 2109 1697 Fragments arranged by size: 2151 2109 1940 1697 1224 1116 226 152 AseI AT'TA_AT Cuts at: 160 1032 9169 160 Size: 872 8137 1606 Fragments arranged by size: 8137 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 10615 AvaI C'yCGr_G Cuts at: 1870 1943 2026 2182 4248 5252 5448 6718 7940 Size: 73 83 156 2066 1004 196 1270 1222 Cuts at: 7940 1870 Size: 4545 Fragments arranged by size: 4545 2066 1270 1222 1004 196 156 83 73 BamHI G'GATC_C Cuts at: 2574 6006 7945 8312 2574 Size: 3432 1939 367 4877 Fragments arranged by size: 4877 3432 1939 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2562 3248 6590 6764 7936 Size: 325 217 627 775 686 3342 174 1172 Cuts at: 7936 9391 618 Size: 1455 1842 Fragments arranged by size: 3342 1842 1455 1172 775 686 627 325 217 174 BanII G_rGCy'C Cuts at: 1391 2724 2810 4185 4545 4792 5272 5385 6716 Size: 1333 86 1375 360 247 480 113 1331 Cuts at: 6716 6810 7934 1391 Size: 94 1124 4072 Fragments arranged by size: 4072 1375 1333 1331 1124 480 360 247 113 94 86 BbeI G_GCGC'C Cuts at: 3252 6594 3252 Size: 3342 7273 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2315 3655 3849 4689 5419 5845 928 Size: 971 416 1340 194 840 730 426 5698 Fragments arranged by size: 5698 1340 971 840 730 426 416 194 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 8806 8840 1903 Size: 34 50 34 6785 34 3678 Fragments arranged by size: 6785 3678 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3821 4571 8507 9717 3821 Size: 750 3936 1210 4719 Fragments arranged by size: 4719 3936 1210 750 BclI T'GATC_A Cuts at: 1548 3909 4596 1548 Size: 2361 687 7567 Fragments arranged by size: 7567 2361 687 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2259 2550 4695 Size: 107 122 71 631 605 587 291 2145 Cuts at: 4695 9226 136 Size: 4531 1525 Fragments arranged by size: 4531 2145 1525 631 605 587 291 122 107 71 BglII A'GATC_T Cuts at: 2673 6096 10610 2673 Size: 3423 4514 2678 Fragments arranged by size: 4514 3423 2678 BlpI GC'TnA_GC Cuts at: 4832 8032 4832 Size: 3200 7415 Bme1580I G_kGCm'C Cuts at: 1790 2772 3147 3944 4792 5370 8676 9832 1790 Size: 982 375 797 848 578 3306 1156 2573 Fragments arranged by size: 3306 2573 1156 982 848 797 578 375 BmgBI CAC'GTC Cuts at: 1318 3711 3945 4828 6058 6078 6157 6316 1318 Size: 2393 234 883 1230 20 79 159 5617 Fragments arranged by size: 5617 2393 1230 883 234 159 79 20 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3303 6519 6597 Size: 416 53 83 399 24 1886 3216 78 Cuts at: 6597 6642 8346 9304 442 Size: 45 1704 958 1753 Fragments arranged by size: 3216 1886 1753 1704 958 416 399 83 78 53 45 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 10615 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6829 6829 Size: 10615 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2918 3551 3689 4199 4941 5507 6032 6158 Size: 1679 633 138 510 742 566 525 126 Cuts at: 6158 7686 9275 1239 Size: 1528 1589 2579 Fragments arranged by size: 2579 1679 1589 1528 742 633 566 525 510 138 126 Bpu10I CC'TnA_GC Cuts at: 4901 4901 Size: 10615 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2996 6721 8740 9605 9903 2996 Size: 3725 2019 865 298 3708 Fragments arranged by size: 3725 3708 2019 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6370 6705 9278 882 Size: 617 4871 335 2573 2219 Fragments arranged by size: 4871 2573 2219 617 335 BsaAI yAC'GTr Cuts at: 493 1204 2293 8406 493 Size: 711 1089 6113 2702 Fragments arranged by size: 6113 2702 1089 711 BsaBI GATnn'nnATC Cuts at: 4343 5966 6242 10609 4343 Size: 1623 276 4367 4349 Fragments arranged by size: 4367 4349 1623 276 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3004 3249 3660 6591 Size: 53 83 186 936 1471 245 411 2931 Cuts at: 6591 8422 8804 9439 275 Size: 1831 382 635 1451 Fragments arranged by size: 2931 1831 1471 1451 936 635 411 382 245 186 83 53 BsaWI w'CCGG_w Cuts at: 2086 4319 4416 5673 9048 9446 9720 9867 10278 Size: 2233 97 1257 3375 398 274 147 411 Cuts at: 10278 2086 Size: 2423 Fragments arranged by size: 3375 2423 2233 1257 411 398 274 147 97 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 5207 5237 6146 6176 7810 Size: 30 165 30 3804 30 909 30 1634 Cuts at: 7810 7840 1178 Size: 30 3953 Fragments arranged by size: 3953 3804 1634 909 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2492 2711 2921 4034 4255 5186 5831 7922 Size: 1306 219 210 1113 221 931 645 2091 Cuts at: 7922 1186 Size: 3879 Fragments arranged by size: 3879 2091 1306 1113 931 645 221 219 210 BseYI C'CCAG_C Cuts at: 2710 5359 6109 6382 6776 6926 6947 6968 6989 Size: 2649 750 273 394 150 21 21 21 Cuts at: 6989 7010 7031 7094 7115 7136 7157 7199 7283 Size: 21 21 63 21 21 21 42 84 Cuts at: 7283 7304 7388 7472 7484 7493 7547 9818 10314 Size: 21 84 84 12 9 54 2271 496 Cuts at: 10314 2710 Size: 3011 Fragments arranged by size: 3011 2649 2271 750 496 394 273 150 84 84 84 63 54 42 21 21 21 21 21 21 21 21 21 12 9 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2247 3380 3638 4685 5000 1163 Size: 573 511 1133 258 1047 315 6778 Fragments arranged by size: 6778 1133 1047 573 511 315 258 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6402 8065 8826 9854 1540 Size: 275 186 4401 1663 761 1028 2301 Fragments arranged by size: 4401 2301 1663 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4902 5385 5555 5775 8676 8761 9832 4902 Size: 483 170 220 2901 85 1071 5685 Fragments arranged by size: 5685 2901 1071 483 220 170 85 BsiWI C'GTAC_G Cuts at: 6067 6067 Size: 10615 BsmI GAATG_Cn' Cuts at: 3429 4233 4975 8126 8219 3429 Size: 804 742 3151 93 5825 Fragments arranged by size: 5825 3151 804 742 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2132 3527 3651 6008 7095 2132 Size: 1395 124 2357 1087 5652 Fragments arranged by size: 5652 2357 1395 1087 124 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2724 2772 2810 3147 3944 4185 4545 Size: 399 934 48 38 337 797 241 360 Cuts at: 4545 4792 4902 5272 5370 5385 5555 5775 6716 Size: 247 110 370 98 15 170 220 941 Cuts at: 6716 6810 7934 8676 8761 9832 1391 Size: 94 1124 742 85 1071 2174 Fragments arranged by size: 2174 1124 1071 941 934 797 742 399 370 360 337 247 241 220 170 110 98 94 85 48 38 15 BspEI T'CCGG_A Cuts at: 2086 4319 10278 2086 Size: 2233 5959 2423 Fragments arranged by size: 5959 2423 2233 BspHI T'CATG_A Cuts at: 3988 5787 6285 8504 10234 3988 Size: 1799 498 2219 1730 4369 Fragments arranged by size: 4369 2219 1799 1730 498 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3330 3525 4959 5022 5145 5305 6805 7970 Size: 2486 195 1434 63 123 160 1500 1165 Cuts at: 7970 844 Size: 3489 Fragments arranged by size: 3489 2486 1500 1434 1165 195 160 123 63 BsrBI CCG'CTC Cuts at: 1394 1823 2203 2776 3150 3673 4864 4876 5447 Size: 429 380 573 374 523 1191 12 571 Cuts at: 5447 6142 6813 8502 1394 Size: 695 671 1689 3507 Fragments arranged by size: 3507 1689 1191 695 671 573 571 523 429 380 374 12 BsrDI GCAATG_nn' Cuts at: 65 1248 2887 2910 3153 4080 4356 5241 9110 Size: 1183 1639 23 243 927 276 885 3869 Cuts at: 9110 9284 65 Size: 174 1396 Fragments arranged by size: 3869 1639 1396 1183 927 885 276 243 174 23 BsrFI r'CCGG_y Cuts at: 1700 1810 3329 3381 3733 4490 6401 6500 6635 Size: 110 1519 52 352 757 1911 99 135 Cuts at: 6635 9259 9446 1700 Size: 2624 187 2869 Fragments arranged by size: 2869 2624 1911 1519 757 352 187 135 110 99 52 BsrGI T'GTAC_A Cuts at: 96 5925 96 Size: 5829 4786 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 10615 BssSI C'ACGA_G Cuts at: 8675 9687 8675 Size: 1012 9603 BstAPI GCAn_nnn'nTGC Cuts at: 794 6411 6581 794 Size: 5617 170 4828 Fragments arranged by size: 5617 4828 170 BstBI TT'CG_AA Cuts at: 9419 9419 Size: 10615 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 10615 BstXI CCAn_nnnn'nTGG Cuts at: 2956 3889 5029 5333 6532 2956 Size: 933 1140 304 1199 7039 Fragments arranged by size: 7039 1199 1140 933 304 BstYI r'GATC_y Cuts at: 1972 2524 2574 2643 2673 3546 6006 6096 6459 Size: 552 50 69 30 873 2460 90 363 Cuts at: 6459 7945 8312 8697 8714 10166 10252 10264 10610 Size: 1486 367 385 17 1452 86 12 346 Cuts at: 10610 1972 Size: 1977 Fragments arranged by size: 2460 1977 1486 1452 873 552 385 367 363 346 90 86 69 50 30 17 12 BstZ17I GTA'TAC Cuts at: 7563 7563 Size: 10615 Bsu36I CC'TnA_GG Cuts at: 2511 2511 Size: 10615 BtgI C'CryG_G Cuts at: 513 1065 2545 2952 3437 3561 4462 4524 4605 Size: 552 1480 407 485 124 901 62 81 Cuts at: 4605 5685 6531 513 Size: 1080 846 4597 Fragments arranged by size: 4597 1480 1080 901 846 552 485 407 124 81 62 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 5811 487 Size: 683 56 4585 5291 Fragments arranged by size: 5291 4585 683 56 BtsI GCAGTG_nn' Cuts at: 3642 8131 8925 8945 3642 Size: 4489 794 20 5312 Fragments arranged by size: 5312 4489 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 10615 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 10580 DraI TTT'AAA Cuts at: 7919 7919 Size: 10615 DraIII CAC_nnn'GTG Cuts at: 5151 6341 6761 5151 Size: 1190 420 9005 Fragments arranged by size: 9005 1190 420 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4556 4931 9622 809 Size: 507 3240 375 4691 1802 Fragments arranged by size: 4691 3240 1802 507 375 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2209 2251 2260 Size: 54 1425 50 275 18 379 42 9 Cuts at: 2260 2274 2735 2946 3558 3735 6229 6240 6399 Size: 14 461 211 612 177 2494 11 159 Cuts at: 6399 8062 8951 10580 8 Size: 1663 889 1629 43 Fragments arranged by size: 2494 1663 1629 1425 889 612 461 379 275 211 177 159 54 50 43 42 18 14 11 9 EagI C'GGCC_G Cuts at: 1537 1812 6399 8062 1537 Size: 275 4587 1663 4090 Fragments arranged by size: 4587 4090 1663 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4010 5129 5817 8545 1353 Size: 2657 1119 688 2728 3423 Fragments arranged by size: 3423 2728 2657 1119 688 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 9202 9576 9722 387 Size: 1064 138 7613 374 146 1280 Fragments arranged by size: 7613 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 5383 5383 Size: 10615 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2117 2918 3041 3551 3689 4199 4941 Size: 393 485 801 123 510 138 510 742 Cuts at: 4941 5507 6032 6158 6658 7686 8678 9275 10062 Size: 566 525 126 500 1028 992 597 787 Cuts at: 10062 10330 1239 Size: 268 1524 Fragments arranged by size: 1524 1028 992 801 787 742 597 566 525 510 510 500 485 393 268 138 126 123 EcoNI CCTnn'n_nnAGG Cuts at: 2447 4049 4820 6756 2447 Size: 1602 771 1936 6306 Fragments arranged by size: 6306 1936 1602 771 EcoO109I rG'GnC_Cy Cuts at: 3724 4788 4789 4813 5992 6133 6723 6731 3724 Size: 1064 1 24 1179 141 590 8 7608 Fragments arranged by size: 7608 1179 1064 590 141 24 8 1 EcoRI G'AATT_C Cuts at: 5984 7924 8302 5984 Size: 1940 378 8297 Fragments arranged by size: 8297 1940 378 EcoRV GAT'ATC Cuts at: 2389 2389 Size: 10615 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3793 4783 6313 3793 Size: 990 1530 8095 Fragments arranged by size: 8095 1530 990 FspI TGC'GCA Cuts at: 2051 3224 3641 5247 5280 9121 2051 Size: 1173 417 1606 33 3841 3545 Fragments arranged by size: 3841 3545 1606 1173 417 33 HaeII r_GCGC'y Cuts at: 1678 3252 6594 9762 1678 Size: 1574 3342 3168 2531 Fragments arranged by size: 3342 3168 2531 1574 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2230 2262 2851 2883 3403 Size: 32 135 32 658 32 589 32 520 Cuts at: 3403 3435 3531 3563 4411 4443 5207 5239 6243 Size: 32 96 32 848 32 764 32 1004 Cuts at: 6243 6275 9256 9288 9330 9362 1373 Size: 32 2981 32 42 32 2626 Fragments arranged by size: 2981 2626 1004 848 764 658 589 520 135 96 42 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3708 5096 6262 7959 8205 669 Size: 988 281 1770 1388 1166 1697 246 3079 Fragments arranged by size: 3079 1770 1697 1388 1166 988 281 246 HindIII A'AGCT_T Cuts at: 748 2162 748 Size: 1414 9201 HpaI GTT'AAC Cuts at: 8205 8205 Size: 10615 KasI G'GCGC_C Cuts at: 3248 6590 3248 Size: 3342 7273 KpnI G_GTAC'C Cuts at: 2566 7940 2566 Size: 5374 5241 MfeI C'AATT_G Cuts at: 3805 8214 3805 Size: 4409 6206 MluI A'CGCG_T Cuts at: 10491 10491 Size: 10615 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4038 4348 4764 4839 5034 5898 6920 7277 Size: 2733 310 416 75 195 864 1022 357 Cuts at: 7277 7403 7475 9729 9913 1305 Size: 126 72 2254 184 2007 Fragments arranged by size: 2733 2254 2007 1022 864 416 357 310 195 184 126 75 72 MscI TGG'CCA Cuts at: 10 64 1489 1832 2253 2262 2276 2948 3560 Size: 54 1425 343 421 9 14 672 612 Cuts at: 3560 6231 6242 10 Size: 2671 11 4383 Fragments arranged by size: 4383 2671 1425 672 612 421 343 54 14 11 9 MslI CAynn'nnrTG Cuts at: 518 1166 2013 2619 2954 3354 3635 3834 3884 Size: 648 847 606 335 400 281 199 50 Cuts at: 3884 4170 5027 5306 5558 6503 8573 8932 9091 Size: 286 857 279 252 945 2070 359 159 Cuts at: 9091 518 Size: 2042 Fragments arranged by size: 2070 2042 945 857 847 648 606 400 359 335 286 281 279 252 199 159 50 MspA1I CmG'CkG Cuts at: 1606 2047 2214 2872 2900 3028 3950 4588 4669 Size: 441 167 658 28 128 922 638 81 Cuts at: 4669 6391 6669 8031 8708 9856 10101 1606 Size: 1722 278 1362 677 1148 245 2120 Fragments arranged by size: 2120 1722 1362 1148 922 677 658 638 441 278 245 167 128 81 28 NaeI GCC'GGC Cuts at: 3735 4492 6403 6637 3735 Size: 757 1911 234 7713 Fragments arranged by size: 7713 1911 757 234 NarI GG'CG_CC Cuts at: 3249 6591 3249 Size: 3342 7273 NcoI C'CATG_G Cuts at: 513 1065 2545 2952 3437 3561 4524 4605 5685 Size: 552 1480 407 485 124 963 81 1080 Cuts at: 5685 6531 513 Size: 846 4597 Fragments arranged by size: 4597 1480 1080 963 846 552 485 407 124 81 NdeI CA'TA_TG Cuts at: 387 2462 387 Size: 2075 8540 NgoMIV G'CCGG_C Cuts at: 3733 4490 6401 6635 3733 Size: 757 1911 234 7713 Fragments arranged by size: 7713 1911 757 234 NheI G'CTAG_C Cuts at: 1052 1052 Size: 10615 NotI GC'GGCC_GC Cuts at: 8062 8062 Size: 10615 NspI r_CATG'y Cuts at: 1132 1229 2043 2226 2435 3355 5881 5932 6508 Size: 97 814 183 209 920 2526 51 576 Cuts at: 6508 7973 9518 1132 Size: 1465 1545 2229 Fragments arranged by size: 2526 2229 1545 1465 920 814 576 209 183 97 51 PasI CC'CwG_GG Cuts at: 1348 4190 6626 1348 Size: 2842 2436 5337 Fragments arranged by size: 5337 2842 2436 PciI A'CATG_T Cuts at: 2222 2431 3351 9514 2222 Size: 209 920 6163 3323 Fragments arranged by size: 6163 3323 920 209 PflMI CCAn_nnn'nTGG Cuts at: 1108 3556 4525 4720 6238 1108 Size: 2448 969 195 1518 5485 Fragments arranged by size: 5485 2448 1518 969 195 PfoI T'CCnGG_A Cuts at: 3549 3666 6009 3549 Size: 117 2343 8155 Fragments arranged by size: 8155 2343 117 PmeI GTTT'AAAC Cuts at: 7919 7919 Size: 10615 PmlI CAC'GTG Cuts at: 2293 2293 Size: 10615 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4745 4777 5213 5245 8684 8716 10197 10229 4745 Size: 32 436 32 3439 32 1481 32 5131 Fragments arranged by size: 5131 3439 1481 436 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3724 4813 6723 6731 3724 Size: 1089 1910 8 7608 Fragments arranged by size: 7608 1910 1089 8 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 10615 PsiI TTA'TAA Cuts at: 8185 8185 Size: 10615 PspOMI G'GGCC_C Cuts at: 4788 4788 Size: 10615 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3224 3256 3224 Size: 32 10583 PstI C_TGCA'G Cuts at: 830 3327 4809 5901 6000 7967 830 Size: 2497 1482 1092 99 1967 3478 Fragments arranged by size: 3478 2497 1967 1482 1092 99 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 10615 PvuII CAG'CTG Cuts at: 2872 3950 4669 6391 2872 Size: 1078 719 1722 7096 Fragments arranged by size: 7096 1722 1078 719 SacI G_AGCT'C Cuts at: 5385 5385 Size: 10615 SalI G'TCGA_C Cuts at: 1936 3706 5094 7957 1936 Size: 1770 1388 2863 4594 Fragments arranged by size: 4594 2863 1770 1388 SanDI GG'GwC_CC Cuts at: 6723 6723 Size: 10615 SapI GCTCTTCn'nnn_ Cuts at: 5129 5129 Size: 10615 SbfI CC_TGCA'GG Cuts at: 5901 6000 7967 5901 Size: 99 1967 8549 Fragments arranged by size: 8549 1967 99 ScaI AGT'ACT Cuts at: 7583 7625 7730 7772 8863 10575 7583 Size: 42 105 42 1091 1712 7623 Fragments arranged by size: 7623 1712 1091 105 42 42 SexAI A'CCwGG_T Cuts at: 3132 3793 4656 4815 6757 3132 Size: 661 863 159 1942 6990 Fragments arranged by size: 6990 1942 863 661 159 SfcI C'TryA_G Cuts at: 826 1045 3323 4805 5897 5996 6910 7288 7351 Size: 219 2278 1482 1092 99 914 378 63 Cuts at: 7351 7477 7498 7864 7963 9098 9779 9970 826 Size: 126 21 366 99 1135 681 191 1471 Fragments arranged by size: 2278 1482 1471 1135 1092 914 681 378 366 219 191 126 99 99 63 21 SfiI GGCCn_nnn'nGGCC Cuts at: 2259 2259 Size: 10615 SfoI GGC'GCC Cuts at: 3250 6592 3250 Size: 3342 7273 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 10615 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 10615 SmaI CCC'GGG Cuts at: 1872 5254 6720 7942 1872 Size: 3382 1466 1222 4545 Fragments arranged by size: 4545 3382 1466 1222 SmlI C'TyrA_G Cuts at: 820 1017 1943 3011 4730 5375 5448 6736 8719 Size: 197 926 1068 1719 645 73 1288 1983 Cuts at: 8719 9620 9882 820 Size: 901 262 1553 Fragments arranged by size: 1983 1719 1553 1288 1068 926 901 645 262 197 73 SnaBI TAC'GTA Cuts at: 493 8406 493 Size: 7913 2702 SpeI A'CTAG_T Cuts at: 152 3217 7319 152 Size: 3065 4102 3448 Fragments arranged by size: 4102 3448 3065 SphI G_CATG'C Cuts at: 1132 2043 7973 1132 Size: 911 5930 3774 Fragments arranged by size: 5930 3774 911 SspI AAT'ATT Cuts at: 5 52 7604 8539 5 Size: 47 7552 935 2081 Fragments arranged by size: 7552 2081 935 47 StuI AGG'CCT Cuts at: 1517 5195 1517 Size: 3678 6937 StyI C'CwwG_G Cuts at: 513 1065 2403 2545 2952 3437 3561 3882 4524 Size: 552 1338 142 407 485 124 321 642 Cuts at: 4524 4605 5287 5466 5685 6531 6560 8054 513 Size: 81 682 179 219 846 29 1494 3074 Fragments arranged by size: 3074 1494 1338 846 682 642 552 485 407 321 219 179 142 124 81 29 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3842 4516 5318 5351 5569 6524 6538 Size: 1255 2262 674 802 33 218 955 14 Cuts at: 6538 6940 6961 6994 7057 7150 7204 7225 7246 Size: 402 21 33 63 93 54 21 21 Cuts at: 7246 7297 7318 7402 7486 7528 7624 7666 8642 Size: 51 21 84 84 42 96 42 976 Cuts at: 8642 8659 8812 8997 9442 325 Size: 17 153 185 445 1498 Fragments arranged by size: 2262 1498 1255 976 955 802 674 445 402 218 185 153 96 93 84 84 63 54 51 42 42 33 33 21 21 21 21 17 14 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3255 5925 7518 Size: 274 80 33 51 1443 1278 2670 1593 Cuts at: 7518 7581 7623 7728 7770 8861 10573 96 Size: 63 42 105 42 1091 1712 138 Fragments arranged by size: 2670 1712 1593 1443 1278 1091 274 138 105 80 63 51 42 42 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3389 3421 5126 5158 5462 5494 6146 6178 6556 Size: 32 1705 32 304 32 652 32 378 Cuts at: 6556 6588 10575 10607 3389 Size: 32 3987 32 3397 Fragments arranged by size: 3987 3397 1705 652 378 304 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 10615 XbaI T'CTAG_A Cuts at: 7951 7951 Size: 10615 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2356 2552 3889 4657 5029 6088 2356 Size: 196 1337 768 372 1059 6883 Fragments arranged by size: 6883 1337 1059 768 372 196 XhoI C'TCGA_G Cuts at: 1943 5448 1943 Size: 3505 7110 XmaI C'CCGG_G Cuts at: 1870 5252 6718 7940 1870 Size: 3382 1466 1222 4545 Fragments arranged by size: 4545 3382 1466 1222 XmnI GAAnn'nnTTC Cuts at: 4760 4982 5321 8744 4760 Size: 222 339 3423 6631 Fragments arranged by size: 6631 3423 339 222 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3661 8423 276 Size: 53 83 186 936 2127 4762 2468 Fragments arranged by size: 4762 2468 2127 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbvCI BfrBI FseI FspAI NruI NsiI PacI PspXI RsrII SacII SrfI SwaI