Restriction map for
pFN21AE3496
(Linear) (Six-base) MAPSORT of: KIEE3496.seq Check: 346 from: 1 to: 5910
KIEE3496.orf
With 182 enzymes: SgfI *
July 16, 2019 14:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1325 1520 3140 4800 5910
Size: 1325 195 1620 1660 1110
Fragments arranged by size:
1660 1620 1325 1110 195
AatII G_ACGT'C
Cuts at: 0 1658 5910
Size: 1658 4252
AccI GT'mk_AC
Cuts at: 0 431 1438 1702 3090 3584 5557 5702 5910
Size: 431 1007 264 1388 494 1973 145 208
Fragments arranged by size:
1973 1388 1007 494 431 264 208 145
Acc65I G'GTAC_C
Cuts at: 0 557 5910
Size: 557 5353
AclI AA'CG_TT
Cuts at: 0 3493 5910
Size: 3493 2417
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 112 1036 4653 5910
Size: 112 924 3617 1257
Fragments arranged by size:
3617 1257 924 112
AflII C'TTAA_G
Cuts at: 0 2725 3370 5910
Size: 2725 645 2540
Fragments arranged by size:
2725 2540 645
AflIII A'CryG_T
Cuts at: 0 217 426 1346 5910
Size: 217 209 920 4564
Fragments arranged by size:
4564 920 217 209
AhdI GACnn_n'nnGTC
Cuts at: 0 239 4252 5910
Size: 239 4013 1658
Fragments arranged by size:
4013 1658 239
AleI CACnn'nnGTG
Cuts at: 0 8 949 1349 1630 1879 3553 5910
Size: 8 941 400 281 249 1674 2357
Fragments arranged by size:
2357 1674 941 400 281 249 8
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 821 1300 4452 5910
Size: 821 479 3152 1458
Fragments arranged by size:
3152 1458 821 479
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 472 504 561 593 5910
Size: 472 32 57 32 5317
Fragments arranged by size:
5317 472 57 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1524 1551 2715 5910
Size: 1524 27 1164 3195
Fragments arranged by size:
3195 1524 1164 27
ApaI G_GGCC'C
Cuts at: 0 2787 5910
Size: 2787 3123
ApoI r'AATT_y
Cuts at: 0 1639 2863 3979 5910
Size: 1639 1224 1116 1931
Fragments arranged by size:
1931 1639 1224 1116
AvaI C'yCGr_G
Cuts at: 0 21 177 2243 3247 3443 4713 5910
Size: 21 156 2066 1004 196 1270 1197
Fragments arranged by size:
2066 1270 1197 1004 196 156 21
BamHI G'GATC_C
Cuts at: 0 569 4001 5910
Size: 569 3432 1909
Fragments arranged by size:
3432 1909 569
BanI G'GyrC_C
Cuts at: 0 557 1243 4585 4759 5910
Size: 557 686 3342 174 1151
Fragments arranged by size:
3342 1151 686 557 174
BanII G_rGCy'C
Cuts at: 0 719 805 2180 2540 2787 3267 3380 4711
Size: 719 86 1375 360 247 480 113 1331
Cuts at: 4711 4805 5910
Size: 94 1105
Fragments arranged by size:
1375 1331 1105 719 480 360 247 113
94 86
BbeI G_GCGC'C
Cuts at: 0 1247 4589 5910
Size: 1247 3342 1321
Fragments arranged by size:
3342 1321 1247
BbsI GAAGACnn'nnnn_
Cuts at: 0 310 1650 1844 2684 3414 3840 5910
Size: 310 1340 194 840 730 426 2070
Fragments arranged by size:
2070 1340 840 730 426 310 194
BciVI GTATCCnnnnn_n'
Cuts at: 0 1816 2566 5910
Size: 1816 750 3344
Fragments arranged by size:
3344 1816 750
BclI T'GATC_A
Cuts at: 0 1904 2591 5910
Size: 1904 687 3319
Fragments arranged by size:
3319 1904 687
BglI GCCn_nnn'nGGC
Cuts at: 0 254 545 2690 5910
Size: 254 291 2145 3220
Fragments arranged by size:
3220 2145 291 254
BglII A'GATC_T
Cuts at: 0 668 4091 5910
Size: 668 3423 1819
Fragments arranged by size:
3423 1819 668
BlpI GC'TnA_GC
Cuts at: 0 2827 5910
Size: 2827 3083
Bme1580I G_kGCm'C
Cuts at: 0 767 1142 1939 2787 3365 5910
Size: 767 375 797 848 578 2545
Fragments arranged by size:
2545 848 797 767 578 375
BmgBI CAC'GTC
Cuts at: 0 1706 1940 2823 4053 4073 4152 4311 5910
Size: 1706 234 883 1230 20 79 159 1599
Fragments arranged by size:
1706 1599 1230 883 234 159 79 20
BmrI ACTGGGnnnn_n'
Cuts at: 0 1298 4514 4592 4637 5910
Size: 1298 3216 78 45 1273
Fragments arranged by size:
3216 1298 1273 78 45
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 4824 5910
Size: 4824 1086
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 913 1546 1684 2194 2936 3502 4027 4153
Size: 913 633 138 510 742 566 525 126
Cuts at: 4153 5681 5910
Size: 1528 229
Fragments arranged by size:
1528 913 742 633 566 525 510 229
138 126
Bpu10I CC'TnA_GC
Cuts at: 0 2896 5910
Size: 2896 3014
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 991 4716 5910
Size: 991 3725 1194
Fragments arranged by size:
3725 1194 991
BsaI GGTCTCn'nnnn_
Cuts at: 0 4365 4700 5910
Size: 4365 335 1210
Fragments arranged by size:
4365 1210 335
BsaAI yAC'GTr
Cuts at: 0 288 5910
Size: 288 5622
BsaBI GATnn'nnATC
Cuts at: 0 2338 3961 4237 5910
Size: 2338 1623 276 1673
Fragments arranged by size:
2338 1673 1623 276
BsaHI Gr'CG_yC
Cuts at: 0 999 1244 1655 4586 5910
Size: 999 245 411 2931 1324
Fragments arranged by size:
2931 1324 999 411 245
BsaWI w'CCGG_w
Cuts at: 0 81 2314 2411 3668 5910
Size: 81 2233 97 1257 2242
Fragments arranged by size:
2242 2233 1257 97 81
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 3202 3232 4141 4171 5805 5835 5910
Size: 3202 30 909 30 1634 30 75
Fragments arranged by size:
3202 1634 909 75 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 487 706 916 2029 2250 3181 3826 5910
Size: 487 219 210 1113 221 931 645 2084
Fragments arranged by size:
2084 1113 931 645 487 221 219 210
BseYI C'CCAG_C
Cuts at: 0 705 3354 4104 4377 4771 4921 4942 4963
Size: 705 2649 750 273 394 150 21 21
Cuts at: 4963 4984 5005 5026 5089 5110 5131 5152 5194
Size: 21 21 21 63 21 21 21 42
Cuts at: 5194 5278 5299 5383 5467 5479 5488 5542 5910
Size: 84 21 84 84 12 9 54 368
Fragments arranged by size:
2649 750 705 394 368 273 150 84
84 84 63 54 42 21 21 21
21 21 21 21 21 21 12 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 242 1375 1633 2680 2995 5910
Size: 242 1133 258 1047 315 2915
Fragments arranged by size:
2915 1133 1047 315 258 242
BsiEI CG_ry'CG
Cuts at: 0 4397 5910
Size: 4397 1513
BsiHKAI G_wGCw'C
Cuts at: 0 2897 3380 3550 3770 5910
Size: 2897 483 170 220 2140
Fragments arranged by size:
2897 2140 483 220 170
BsiWI C'GTAC_G
Cuts at: 0 4062 5910
Size: 4062 1848
BsmI GAATG_Cn'
Cuts at: 0 1424 2228 2970 5910
Size: 1424 804 742 2940
Fragments arranged by size:
2940 1424 804 742
BsmBI CGTCTCn'nnnn_
Cuts at: 0 127 1522 1646 4003 5090 5910
Size: 127 1395 124 2357 1087 820
Fragments arranged by size:
2357 1395 1087 820 127 124
Bsp1286I G_dGCh'C
Cuts at: 0 719 767 805 1142 1939 2180 2540 2787
Size: 719 48 38 337 797 241 360 247
Cuts at: 2787 2897 3267 3365 3380 3550 3770 4711 4805
Size: 110 370 98 15 170 220 941 94
Cuts at: 4805 5910
Size: 1105
Fragments arranged by size:
1105 941 797 719 370 360 337 247
241 220 170 110 98 94 48 38
15
BspEI T'CCGG_A
Cuts at: 0 81 2314 5910
Size: 81 2233 3596
Fragments arranged by size:
3596 2233 81
BspHI T'CATG_A
Cuts at: 0 1983 3782 4280 5910
Size: 1983 1799 498 1630
Fragments arranged by size:
1983 1799 1630 498
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1325 1520 2954 3017 3140 3300 4800 5910
Size: 1325 195 1434 63 123 160 1500 1110
Fragments arranged by size:
1500 1434 1325 1110 195 160 123 63
BsrBI CCG'CTC
Cuts at: 0 198 771 1145 1668 2859 2871 3442 4137
Size: 198 573 374 523 1191 12 571 695
Cuts at: 4137 4808 5910
Size: 671 1102
Fragments arranged by size:
1191 1102 695 671 573 571 523 374
198 12
BsrDI GCAATG_nn'
Cuts at: 0 882 905 1148 2075 2351 3236 5910
Size: 882 23 243 927 276 885 2674
Fragments arranged by size:
2674 927 885 882 276 243 23
BsrFI r'CCGG_y
Cuts at: 0 1324 1376 1728 2485 4396 4495 4630 5910
Size: 1324 52 352 757 1911 99 135 1280
Fragments arranged by size:
1911 1324 1280 757 352 135 99 52
BsrGI T'GTAC_A
Cuts at: 0 3920 5910
Size: 3920 1990
BstAPI GCAn_nnn'nTGC
Cuts at: 0 4406 4576 5910
Size: 4406 170 1334
Fragments arranged by size:
4406 1334 170
BstXI CCAn_nnnn'nTGG
Cuts at: 0 951 1884 3024 3328 4527 5910
Size: 951 933 1140 304 1199 1383
Fragments arranged by size:
1383 1199 1140 951 933 304
BstYI r'GATC_y
Cuts at: 0 519 569 638 668 1541 4001 4091 4454
Size: 519 50 69 30 873 2460 90 363
Cuts at: 4454 5910
Size: 1456
Fragments arranged by size:
2460 1456 873 519 363 90 69 50
30
BstZ17I GTA'TAC
Cuts at: 0 5558 5910
Size: 5558 352
Bsu36I CC'TnA_GG
Cuts at: 0 506 5910
Size: 506 5404
BtgI C'CryG_G
Cuts at: 0 540 947 1432 1556 2457 2519 2600 3680
Size: 540 407 485 124 901 62 81 1080
Cuts at: 3680 4526 5910
Size: 846 1384
Fragments arranged by size:
1384 1080 901 846 540 485 407 124
81 62
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 3806 5910
Size: 3806 2104
BtsI GCAGTG_nn'
Cuts at: 0 1637 5910
Size: 1637 4273
DraIII CAC_nnn'GTG
Cuts at: 0 3146 4336 4756 5910
Size: 3146 1190 420 1154
Fragments arranged by size:
3146 1190 1154 420
DrdI GACnn_nn'nnGTC
Cuts at: 0 2551 2926 5910
Size: 2551 375 2984
Fragments arranged by size:
2984 2551 375
EaeI y'GGCC_r
Cuts at: 0 204 246 255 269 730 941 1553 1730
Size: 204 42 9 14 461 211 612 177
Cuts at: 1730 4224 4235 4394 5910
Size: 2494 11 159 1516
Fragments arranged by size:
2494 1516 612 461 211 204 177 159
42 14 11 9
EagI C'GGCC_G
Cuts at: 0 4394 5910
Size: 4394 1516
EarI CTCTTCn'nnn_
Cuts at: 0 2005 3124 3812 5910
Size: 2005 1119 688 2098
Fragments arranged by size:
2098 2005 1119 688
EcoICRI GAG'CTC
Cuts at: 0 3378 5910
Size: 3378 2532
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 112 913 1036 1546 1684 2194 2936 3502
Size: 112 801 123 510 138 510 742 566
Cuts at: 3502 4027 4153 4653 5681 5910
Size: 525 126 500 1028 229
Fragments arranged by size:
1028 801 742 566 525 510 510 500
229 138 126 123 112
EcoNI CCTnn'n_nnAGG
Cuts at: 0 442 2044 2815 4751 5910
Size: 442 1602 771 1936 1159
Fragments arranged by size:
1936 1602 1159 771 442
EcoO109I rG'GnC_Cy
Cuts at: 0 1719 2783 2784 2808 3987 4128 4718 4726
Size: 1719 1064 1 24 1179 141 590 8
Cuts at: 4726 5910
Size: 1184
Fragments arranged by size:
1719 1184 1179 1064 590 141 24 8
1
EcoRI G'AATT_C
Cuts at: 0 3979 5910
Size: 3979 1931
EcoRV GAT'ATC
Cuts at: 0 384 5910
Size: 384 5526
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1788 2778 4308 5910
Size: 1788 990 1530 1602
Fragments arranged by size:
1788 1602 1530 990
FspI TGC'GCA
Cuts at: 0 46 1219 1636 3242 3275 5910
Size: 46 1173 417 1606 33 2635
Fragments arranged by size:
2635 1606 1173 417 46 33
HaeII r_GCGC'y
Cuts at: 0 1247 4589 5910
Size: 1247 3342 1321
Fragments arranged by size:
3342 1321 1247
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 225 257 846 878 1398 1430 1526 1558
Size: 225 32 589 32 520 32 96 32
Cuts at: 1558 2406 2438 3202 3234 4238 4270 5910
Size: 848 32 764 32 1004 32 1640
Fragments arranged by size:
1640 1004 848 764 589 520 225 96
32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 1703 3091 4257 5910
Size: 1703 1388 1166 1653
Fragments arranged by size:
1703 1653 1388 1166
HindIII A'AGCT_T
Cuts at: 0 157 5910
Size: 157 5753
KasI G'GCGC_C
Cuts at: 0 1243 4585 5910
Size: 1243 3342 1325
Fragments arranged by size:
3342 1325 1243
KpnI G_GTAC'C
Cuts at: 0 561 5910
Size: 561 5349
MfeI C'AATT_G
Cuts at: 0 1800 5910
Size: 1800 4110
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2033 2343 2759 2834 3029 3893 4915 5272
Size: 2033 310 416 75 195 864 1022 357
Cuts at: 5272 5398 5470 5910
Size: 126 72 440
Fragments arranged by size:
2033 1022 864 440 416 357 310 195
126 75 72
MscI TGG'CCA
Cuts at: 0 248 257 271 943 1555 4226 4237 5910
Size: 248 9 14 672 612 2671 11 1673
Fragments arranged by size:
2671 1673 672 612 248 14 11 9
MslI CAynn'nnrTG
Cuts at: 0 8 614 949 1349 1630 1829 1879 2165
Size: 8 606 335 400 281 199 50 286
Cuts at: 2165 3022 3301 3553 4498 5910
Size: 857 279 252 945 1412
Fragments arranged by size:
1412 945 857 606 400 335 286 281
279 252 199 50 8
MspA1I CmG'CkG
Cuts at: 0 42 209 867 895 1023 1945 2583 2664
Size: 42 167 658 28 128 922 638 81
Cuts at: 2664 4386 4664 5910
Size: 1722 278 1246
Fragments arranged by size:
1722 1246 922 658 638 278 167 128
81 42 28
NaeI GCC'GGC
Cuts at: 0 1730 2487 4398 4632 5910
Size: 1730 757 1911 234 1278
Fragments arranged by size:
1911 1730 1278 757 234
NarI GG'CG_CC
Cuts at: 0 1244 4586 5910
Size: 1244 3342 1324
Fragments arranged by size:
3342 1324 1244
NcoI C'CATG_G
Cuts at: 0 540 947 1432 1556 2519 2600 3680 4526
Size: 540 407 485 124 963 81 1080 846
Cuts at: 4526 5910
Size: 1384
Fragments arranged by size:
1384 1080 963 846 540 485 407 124
81
NdeI CA'TA_TG
Cuts at: 0 457 5910
Size: 457 5453
NgoMIV G'CCGG_C
Cuts at: 0 1728 2485 4396 4630 5910
Size: 1728 757 1911 234 1280
Fragments arranged by size:
1911 1728 1280 757 234
NspI r_CATG'y
Cuts at: 0 38 221 430 1350 3876 3927 4503 5910
Size: 38 183 209 920 2526 51 576 1407
Fragments arranged by size:
2526 1407 920 576 209 183 51 38
PasI CC'CwG_GG
Cuts at: 0 2185 4621 5910
Size: 2185 2436 1289
Fragments arranged by size:
2436 2185 1289
PciI A'CATG_T
Cuts at: 0 217 426 1346 5910
Size: 217 209 920 4564
Fragments arranged by size:
4564 920 217 209
PflMI CCAn_nnn'nTGG
Cuts at: 0 1551 2520 2715 4233 5910
Size: 1551 969 195 1518 1677
Fragments arranged by size:
1677 1551 1518 969 195
PfoI T'CCnGG_A
Cuts at: 0 1544 1661 4004 5910
Size: 1544 117 2343 1906
Fragments arranged by size:
2343 1906 1544 117
PmlI CAC'GTG
Cuts at: 0 288 5910
Size: 288 5622
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2740 2772 3208 3240 5910
Size: 2740 32 436 32 2670
Fragments arranged by size:
2740 2670 436 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 1719 2808 4718 4726 5910
Size: 1719 1089 1910 8 1184
Fragments arranged by size:
1910 1719 1184 1089 8
PspOMI G'GGCC_C
Cuts at: 0 2783 5910
Size: 2783 3127
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1219 1251 5910
Size: 1219 32 4659
Fragments arranged by size:
4659 1219 32
PstI C_TGCA'G
Cuts at: 0 1322 2804 3896 3995 5910
Size: 1322 1482 1092 99 1915
Fragments arranged by size:
1915 1482 1322 1092 99
PvuII CAG'CTG
Cuts at: 0 867 1945 2664 4386 5910
Size: 867 1078 719 1722 1524
Fragments arranged by size:
1722 1524 1078 867 719
SacI G_AGCT'C
Cuts at: 0 3380 5910
Size: 3380 2530
SalI G'TCGA_C
Cuts at: 0 1701 3089 5910
Size: 1701 1388 2821
Fragments arranged by size:
2821 1701 1388
SanDI GG'GwC_CC
Cuts at: 0 4718 5910
Size: 4718 1192
SapI GCTCTTCn'nnn_
Cuts at: 0 3124 5910
Size: 3124 2786
SbfI CC_TGCA'GG
Cuts at: 0 3896 3995 5910
Size: 3896 99 1915
Fragments arranged by size:
3896 1915 99
ScaI AGT'ACT
Cuts at: 0 5578 5620 5725 5767 5910
Size: 5578 42 105 42 143
Fragments arranged by size:
5578 143 105 42 42
SexAI A'CCwGG_T
Cuts at: 0 1127 1788 2651 2810 4752 5910
Size: 1127 661 863 159 1942 1158
Fragments arranged by size:
1942 1158 1127 863 661 159
SfcI C'TryA_G
Cuts at: 0 1318 2800 3892 3991 4905 5283 5346 5472
Size: 1318 1482 1092 99 914 378 63 126
Cuts at: 5472 5493 5859 5910
Size: 21 366 51
Fragments arranged by size:
1482 1318 1092 914 378 366 126 99
63 51 21
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 254 5910
Size: 254 5656
SfoI GGC'GCC
Cuts at: 0 1245 4587 5910
Size: 1245 3342 1323
Fragments arranged by size:
3342 1323 1245
SmaI CCC'GGG
Cuts at: 0 3249 4715 5910
Size: 3249 1466 1195
Fragments arranged by size:
3249 1466 1195
SmlI C'TyrA_G
Cuts at: 0 1006 2725 3370 3443 4731 5910
Size: 1006 1719 645 73 1288 1179
Fragments arranged by size:
1719 1288 1179 1006 645 73
SpeI A'CTAG_T
Cuts at: 0 1212 5314 5910
Size: 1212 4102 596
Fragments arranged by size:
4102 1212 596
SphI G_CATG'C
Cuts at: 0 38 5910
Size: 38 5872
SspI AAT'ATT
Cuts at: 0 5599 5910
Size: 5599 311
StuI AGG'CCT
Cuts at: 0 3190 5910
Size: 3190 2720
StyI C'CwwG_G
Cuts at: 0 398 540 947 1432 1556 1877 2519 2600
Size: 398 142 407 485 124 321 642 81
Cuts at: 2600 3282 3461 3680 4526 4555 5910
Size: 682 179 219 846 29 1355
Fragments arranged by size:
1355 846 682 642 485 407 398 321
219 179 142 124 81 29
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1837 2511 3313 3346 3564 4519 4533 4935
Size: 1837 674 802 33 218 955 14 402
Cuts at: 4935 4956 4989 5052 5145 5199 5220 5241 5292
Size: 21 33 63 93 54 21 21 51
Cuts at: 5292 5313 5397 5481 5523 5619 5661 5910
Size: 21 84 84 42 96 42 249
Fragments arranged by size:
1837 955 802 674 402 249 218 96
93 84 84 63 54 51 42 42
33 33 21 21 21 21 14
TatI w'GTAC_w
Cuts at: 0 1250 3920 5513 5576 5618 5723 5765 5910
Size: 1250 2670 1593 63 42 105 42 145
Fragments arranged by size:
2670 1593 1250 145 105 63 42 42
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1384 1416 3121 3153 3457 3489 4141 4173
Size: 1384 32 1705 32 304 32 652 32
Cuts at: 4173 4551 4583 5910
Size: 378 32 1327
Fragments arranged by size:
1705 1384 1327 652 378 304 32 32
32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 351 547 1884 2652 3024 4083 5910
Size: 351 196 1337 768 372 1059 1827
Fragments arranged by size:
1827 1337 1059 768 372 351 196
XhoI C'TCGA_G
Cuts at: 0 3443 5910
Size: 3443 2467
XmaI C'CCGG_G
Cuts at: 0 3247 4713 5910
Size: 3247 1466 1197
Fragments arranged by size:
3247 1466 1197
XmnI GAAnn'nnTTC
Cuts at: 0 2755 2977 3316 5910
Size: 2755 222 339 2594
Fragments arranged by size:
2755 2594 339 222
ZraI GAC'GTC
Cuts at: 0 1656 5910
Size: 1656 4254
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AhdI AleI AlfI AloI AlwNI ApaI ApoI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BstAPI
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DraIII
DrdI EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MfeI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NspI PasI PciI PflMI
PfoI PmlI PpiI PpuMI PspOMI PsrI PstI PvuII
SacI SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SmaI SmlI SpeI SphI SspI StuI
StyI TaqII TatI TstI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AgeI ApaLI AscI AseI AsiSI AvrII BaeI
BbvCI BcgI BfrBI BmtI BssHII BssSI BstBI BstEII
ClaI CspCI DraI EciI FseI FspAI HpaI MluI
NheI NotI NruI NsiI PacI PmeI PshAI PsiI
PspXI PvuI RsrII SacII SgfI SgrAI SnaBI SrfI
SwaI Tth111I XbaI