(Linear) (Six-base) MAPSORT of: KIEE5810.seq Check: 5241 from: 1 to: 4365 KIEE5810.orf With 182 enzymes: SgfI * December 5, 2014 13:11 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1458 4365 Size: 1458 2907 AatII G_ACGT'C Cuts at: 0 689 2173 4365 Size: 689 1484 2192 Fragments arranged by size: 2192 1484 689 AccI GT'mk_AC Cuts at: 0 3455 4365 Size: 3455 910 Acc65I G'GTAC_C Cuts at: 0 1460 4365 Size: 1460 2905 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 615 942 967 1627 1840 1904 2843 2891 Size: 615 327 25 660 213 64 939 48 Cuts at: 2891 3190 3387 4365 Size: 299 197 978 Fragments arranged by size: 978 939 660 615 327 299 213 197 64 48 25 AflIII A'CryG_T Cuts at: 0 1736 2265 2337 4076 4248 4365 Size: 1736 529 72 1739 172 117 Fragments arranged by size: 1739 1736 529 172 117 72 AleI CACnn'nnGTG Cuts at: 0 2372 3368 3860 4365 Size: 2372 996 492 505 Fragments arranged by size: 2372 996 505 492 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2732 4365 Size: 2732 1633 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2473 2505 4365 Size: 2473 32 1860 Fragments arranged by size: 2473 1860 32 AlwNI CAG_nnn'CTG Cuts at: 0 168 1215 2187 2845 3174 3682 4365 Size: 168 1047 972 658 329 508 683 Fragments arranged by size: 1047 972 683 658 508 329 168 ApaI G_GGCC'C Cuts at: 0 1324 2320 4365 Size: 1324 996 2045 Fragments arranged by size: 2045 1324 996 ApaLI G'TGCA_C Cuts at: 0 1471 2365 4365 Size: 1471 894 2000 Fragments arranged by size: 2000 1471 894 ApoI r'AATT_y Cuts at: 0 2503 2813 3006 4365 Size: 2503 310 193 1359 Fragments arranged by size: 2503 1359 310 193 AvaI C'yCGr_G Cuts at: 0 30 1769 2319 3581 3874 4365 Size: 30 1739 550 1262 293 491 Fragments arranged by size: 1739 1262 550 491 293 30 BamHI G'GATC_C Cuts at: 0 3239 4365 Size: 3239 1126 BanI G'GyrC_C Cuts at: 0 1460 3370 4226 4365 Size: 1460 1910 856 139 Fragments arranged by size: 1910 1460 856 139 BanII G_rGCy'C Cuts at: 0 1324 1628 1946 2320 3574 4157 4365 Size: 1324 304 318 374 1254 583 208 Fragments arranged by size: 1324 1254 583 374 318 304 208 BbsI GAAGACnn'nnnn_ Cuts at: 0 428 1229 1404 4003 4037 4365 Size: 428 801 175 2599 34 328 Fragments arranged by size: 2599 801 428 328 175 34 BbvCI CC'TCA_GC Cuts at: 0 1937 4365 Size: 1937 2428 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 592 626 4365 Size: 592 34 3739 Fragments arranged by size: 3739 592 34 BciVI GTATCCnnnnn_n' Cuts at: 0 4107 4365 Size: 4107 258 BfrBI ATG'CAT Cuts at: 0 609 4365 Size: 609 3756 BglII A'GATC_T Cuts at: 0 368 753 4365 Size: 368 385 3612 Fragments arranged by size: 3612 385 368 BlpI GC'TnA_GC Cuts at: 0 2110 4365 Size: 2110 2255 Bme1580I G_kGCm'C Cuts at: 0 120 1324 1475 2320 2334 2369 3375 4231 Size: 120 1204 151 845 14 35 1006 856 Cuts at: 4231 4365 Size: 134 Fragments arranged by size: 1204 1006 856 845 151 134 120 35 14 BmgBI CAC'GTC Cuts at: 0 2266 4365 Size: 2266 2099 BmrI ACTGGGnnnn_n' Cuts at: 0 1236 1687 1978 2544 4365 Size: 1236 451 291 566 1821 Fragments arranged by size: 1821 1236 566 451 291 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 882 1341 2737 3663 4365 Size: 882 459 1396 926 702 Fragments arranged by size: 1396 926 882 702 459 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 365 869 1336 2152 2184 2567 2697 2824 Size: 365 504 467 816 32 383 130 127 Cuts at: 2824 3103 3151 3550 4118 4171 4291 4365 Size: 279 48 399 568 53 120 74 Fragments arranged by size: 816 568 504 467 399 383 365 279 130 127 120 74 53 48 32 Bpu10I CC'TnA_GC Cuts at: 0 1937 4365 Size: 1937 2428 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 205 291 1546 2967 4141 4365 Size: 205 86 1255 1421 1174 224 Fragments arranged by size: 1421 1255 1174 224 205 86 BsaI GGTCTCn'nnnn_ Cuts at: 0 382 2673 2918 3431 4365 Size: 382 2291 245 513 934 Fragments arranged by size: 2291 934 513 382 245 BsaAI yAC'GTr Cuts at: 0 558 1737 4251 4365 Size: 558 1179 2514 114 Fragments arranged by size: 2514 1179 558 114 BsaHI Gr'CG_yC Cuts at: 0 686 2170 4365 Size: 686 1484 2195 Fragments arranged by size: 2195 1484 686 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 2255 2285 3227 3257 3343 3373 4365 Size: 2255 30 942 30 86 30 992 Fragments arranged by size: 2255 992 942 86 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 874 1120 1507 1954 2668 3235 3570 3775 Size: 874 246 387 447 714 567 335 205 Cuts at: 3775 3943 3946 4365 Size: 168 3 419 Fragments arranged by size: 874 714 567 447 419 387 335 246 205 168 3 BseYI C'CCAG_C Cuts at: 0 312 1609 1999 2343 3651 3942 4108 4365 Size: 312 1297 390 344 1308 291 166 257 Fragments arranged by size: 1308 1297 390 344 312 291 257 166 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 357 1121 1949 2402 2589 3668 3673 3884 Size: 357 764 828 453 187 1079 5 211 Cuts at: 3884 4365 Size: 481 Fragments arranged by size: 1079 828 764 481 453 357 211 187 5 BsiHKAI G_wGCw'C Cuts at: 0 1169 1375 1475 1778 2273 2285 2369 2885 Size: 1169 206 100 303 495 12 84 516 Cuts at: 2885 3137 4157 4365 Size: 252 1020 208 Fragments arranged by size: 1169 1020 516 495 303 252 208 206 100 84 12 BsmI GAATG_Cn' Cuts at: 0 177 821 1252 4365 Size: 177 644 431 3113 Fragments arranged by size: 3113 644 431 177 Bsp1286I G_dGCh'C Cuts at: 0 120 1169 1324 1375 1475 1628 1778 1946 Size: 120 1049 155 51 100 153 150 168 Cuts at: 1946 2273 2285 2320 2334 2369 2885 3137 3375 Size: 327 12 35 14 35 516 252 238 Cuts at: 3375 3574 4157 4231 4365 Size: 199 583 74 134 Fragments arranged by size: 1049 583 516 327 252 238 199 168 155 153 150 134 120 100 74 51 35 35 14 12 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 780 1458 1472 2936 3848 4365 Size: 780 678 14 1464 912 517 Fragments arranged by size: 1464 912 780 678 517 14 BsrBI CCG'CTC Cuts at: 0 1511 4365 Size: 1511 2854 BsrFI r'CCGG_y Cuts at: 0 3548 3608 4365 Size: 3548 60 757 Fragments arranged by size: 3548 757 60 BsrGI T'GTAC_A Cuts at: 0 2509 3230 4365 Size: 2509 721 1135 Fragments arranged by size: 2509 1135 721 BssSI C'ACGA_G Cuts at: 0 9 1376 1438 2555 4365 Size: 9 1367 62 1117 1810 Fragments arranged by size: 1810 1367 1117 62 9 BstAPI GCAn_nnn'nTGC Cuts at: 0 585 816 1012 1164 1215 2425 4365 Size: 585 231 196 152 51 1210 1940 Fragments arranged by size: 1940 1210 585 231 196 152 51 BstYI r'GATC_y Cuts at: 0 368 753 939 3239 4365 Size: 368 385 186 2300 1126 Fragments arranged by size: 2300 1126 385 368 186 Bsu36I CC'TnA_GG Cuts at: 0 3638 3937 4365 Size: 3638 299 428 Fragments arranged by size: 3638 428 299 BtgI C'CryG_G Cuts at: 0 323 1386 2137 3363 4175 4365 Size: 323 1063 751 1226 812 190 Fragments arranged by size: 1226 1063 812 751 323 190 BtsI GCAGTG_nn' Cuts at: 0 930 1283 2039 2285 3302 3389 3561 3685 Size: 930 353 756 246 1017 87 172 124 Cuts at: 3685 4365 Size: 680 Fragments arranged by size: 1017 930 756 680 353 246 172 124 87 DraIII CAC_nnn'GTG Cuts at: 0 402 2425 4365 Size: 402 2023 1940 Fragments arranged by size: 2023 1940 402 DrdI GACnn_nn'nnGTC Cuts at: 0 13 1279 4365 Size: 13 1266 3086 Fragments arranged by size: 3086 1266 13 EaeI y'GGCC_r Cuts at: 0 1116 1538 1553 2360 3027 3114 3467 3520 Size: 1116 422 15 807 667 87 353 53 Cuts at: 3520 4037 4365 Size: 517 328 Fragments arranged by size: 1116 807 667 517 422 353 328 87 53 15 EarI CTCTTCn'nnn_ Cuts at: 0 634 943 1646 1816 1880 2248 3883 4043 Size: 634 309 703 170 64 368 1635 160 Cuts at: 4043 4105 4365 Size: 62 260 Fragments arranged by size: 1635 703 634 368 309 260 170 160 64 62 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 15 3238 4365 Size: 15 3223 1127 Fragments arranged by size: 3223 1127 15 EcoICRI GAG'CTC Cuts at: 0 4155 4365 Size: 4155 210 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 365 615 869 942 967 1336 1627 1840 Size: 365 250 254 73 25 369 291 213 Cuts at: 1840 1904 2152 2184 2567 2697 2824 2843 2891 Size: 64 248 32 383 130 127 19 48 Cuts at: 2891 3103 3151 3190 3387 3550 4118 4171 4291 Size: 212 48 39 197 163 568 53 120 Cuts at: 4291 4365 Size: 74 Fragments arranged by size: 568 383 369 365 291 254 250 248 213 212 197 163 130 127 120 74 73 64 53 48 48 39 32 25 19 EcoNI CCTnn'n_nnAGG Cuts at: 0 3379 3844 4345 4365 Size: 3379 465 501 20 Fragments arranged by size: 3379 501 465 20 EcoO109I rG'GnC_Cy Cuts at: 0 15 39 59 165 1320 1321 1404 2227 Size: 15 24 20 106 1155 1 83 823 Cuts at: 2227 2316 2410 3408 4365 Size: 89 94 998 957 Fragments arranged by size: 1155 998 957 823 106 94 89 83 24 20 15 1 EcoRV GAT'ATC Cuts at: 0 2096 3899 4365 Size: 2096 1803 466 Fragments arranged by size: 2096 1803 466 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 972 1380 4216 4365 Size: 972 408 2836 149 Fragments arranged by size: 2836 972 408 149 HaeII r_GCGC'y Cuts at: 0 4301 4365 Size: 4301 64 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 289 321 611 643 969 1001 1041 1073 Size: 289 32 290 32 326 32 40 32 Cuts at: 1073 1516 1548 1585 1588 1617 1620 2002 2034 Size: 443 32 37 3 29 3 382 32 Cuts at: 2034 2211 2243 2255 2287 2368 2400 3492 3524 Size: 177 32 12 32 81 32 1092 32 Cuts at: 3524 3616 3648 3658 3690 4365 Size: 92 32 10 32 675 Fragments arranged by size: 1092 675 443 382 326 290 289 177 92 81 40 37 32 32 32 32 32 32 32 32 32 32 32 32 29 12 10 3 3 HincII GTy'rAC Cuts at: 0 2955 3336 3456 3923 4365 Size: 2955 381 120 467 442 Fragments arranged by size: 2955 467 442 381 120 KpnI G_GTAC'C Cuts at: 0 1464 4365 Size: 1464 2901 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 29 4365 Size: 29 4336 MscI TGG'CCA Cuts at: 0 1118 1540 3469 4365 Size: 1118 422 1929 896 Fragments arranged by size: 1929 1118 896 422 MslI CAynn'nnrTG Cuts at: 0 1666 1702 2152 2372 3368 3860 4365 Size: 1666 36 450 220 996 492 505 Fragments arranged by size: 1666 996 505 492 450 220 36 MspA1I CmG'CkG Cuts at: 0 139 385 1215 1269 1609 1963 2301 2573 Size: 139 246 830 54 340 354 338 272 Cuts at: 2573 3576 3679 4138 4365 Size: 1003 103 459 227 Fragments arranged by size: 1003 830 459 354 340 338 272 246 227 139 103 54 NaeI GCC'GGC Cuts at: 0 3610 4365 Size: 3610 755 NcoI C'CATG_G Cuts at: 0 1386 4365 Size: 1386 2979 NdeI CA'TA_TG Cuts at: 0 1341 4365 Size: 1341 3024 NgoMIV G'CCGG_C Cuts at: 0 3608 4365 Size: 3608 757 NsiI A_TGCA'T Cuts at: 0 611 4365 Size: 611 3754 NspI r_CATG'y Cuts at: 0 609 613 682 2341 4066 4080 4365 Size: 609 4 69 1659 1725 14 285 Fragments arranged by size: 1725 1659 609 285 69 14 4 PasI CC'CwG_GG Cuts at: 0 43 1908 1947 2231 4365 Size: 43 1865 39 284 2134 Fragments arranged by size: 2134 1865 284 43 39 PciI A'CATG_T Cuts at: 0 2337 4076 4365 Size: 2337 1739 289 Fragments arranged by size: 2337 1739 289 PfoI T'CCnGG_A Cuts at: 0 2674 4365 Size: 2674 1691 PmlI CAC'GTG Cuts at: 0 4251 4365 Size: 4251 114 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 13 45 4365 Size: 13 32 4320 Fragments arranged by size: 4320 32 13 PpuMI rG'GwC_Cy Cuts at: 0 15 39 165 2227 2410 4365 Size: 15 24 126 2062 183 1955 Fragments arranged by size: 2062 1955 183 126 24 15 PspOMI G'GGCC_C Cuts at: 0 1320 2316 4365 Size: 1320 996 2049 Fragments arranged by size: 2049 1320 996 PspXI vC'TCGA_Gb Cuts at: 0 1769 3874 4365 Size: 1769 2105 491 Fragments arranged by size: 2105 1769 491 PstI C_TGCA'G Cuts at: 0 812 1396 1469 2280 2703 2933 3092 3593 Size: 812 584 73 811 423 230 159 501 Cuts at: 3593 4214 4365 Size: 621 151 Fragments arranged by size: 812 811 621 584 501 423 230 159 151 73 PvuII CAG'CTG Cuts at: 0 139 1215 1269 1609 1963 2301 2573 3679 Size: 139 1076 54 340 354 338 272 1106 Cuts at: 3679 4138 4365 Size: 459 227 Fragments arranged by size: 1106 1076 459 354 340 338 272 227 139 54 SacI G_AGCT'C Cuts at: 0 4157 4365 Size: 4157 208 SalI G'TCGA_C Cuts at: 0 3454 4365 Size: 3454 911 SanDI GG'GwC_CC Cuts at: 0 15 2227 4365 Size: 15 2212 2138 Fragments arranged by size: 2212 2138 15 SapI GCTCTTCn'nnn_ Cuts at: 0 634 1646 4105 4365 Size: 634 1012 2459 260 Fragments arranged by size: 2459 1012 634 260 SbfI CC_TGCA'GG Cuts at: 0 1469 2933 4365 Size: 1469 1464 1432 Fragments arranged by size: 1469 1464 1432 SexAI A'CCwGG_T Cuts at: 0 2421 4365 Size: 2421 1944 SfcI C'TryA_G Cuts at: 0 808 1392 1465 1571 2276 2469 2699 2929 Size: 808 584 73 106 705 193 230 230 Cuts at: 2929 3088 3589 4210 4365 Size: 159 501 621 155 Fragments arranged by size: 808 705 621 584 501 230 230 193 159 155 106 73 SmaI CCC'GGG Cuts at: 0 3583 4365 Size: 3583 782 SmlI C'TyrA_G Cuts at: 0 184 306 1561 1769 2982 3874 4156 4365 Size: 184 122 1255 208 1213 892 282 209 Fragments arranged by size: 1255 1213 892 282 209 208 184 122 SphI G_CATG'C Cuts at: 0 613 682 4365 Size: 613 69 3683 Fragments arranged by size: 3683 613 69 StuI AGG'CCT Cuts at: 0 253 1360 1728 1870 2588 4054 4365 Size: 253 1107 368 142 718 1466 311 Fragments arranged by size: 1466 1107 718 368 311 253 142 StyI C'CwwG_G Cuts at: 0 1386 4365 Size: 1386 2979 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1218 1499 2362 3672 4365 Size: 1218 281 863 1310 693 Fragments arranged by size: 1310 1218 863 693 281 TatI w'GTAC_w Cuts at: 0 804 852 1850 2509 3230 4079 4365 Size: 804 48 998 659 721 849 286 Fragments arranged by size: 998 849 804 721 659 286 48 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2244 2276 3109 3141 4365 Size: 2244 32 833 32 1224 Fragments arranged by size: 2244 1224 833 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1548 4365 Size: 1548 2817 XhoI C'TCGA_G Cuts at: 0 1769 3874 4365 Size: 1769 2105 491 Fragments arranged by size: 2105 1769 491 XmaI C'CCGG_G Cuts at: 0 3581 4365 Size: 3581 784 XmnI GAAnn'nnTTC Cuts at: 0 952 2771 4365 Size: 952 1819 1594 Fragments arranged by size: 1819 1594 952 ZraI GAC'GTC Cuts at: 0 687 2171 4365 Size: 687 1484 2194 Fragments arranged by size: 2194 1484 687 Enzymes that do cut: AarI AatII AccI Acc65I AcuI AflIII AleI AlfI AloI AlwNI ApaI ApaLI ApoI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BfrBI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrFI BsrGI BssSI BstAPI BstYI Bsu36I BtgI BtsI DraIII DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI HaeII Hin4I HincII KpnI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NsiI NspI PasI PciI PfoI PmlI PpiI PpuMI PspOMI PspXI PstI PvuII SacI SalI SanDI SapI SbfI SexAI SfcI SmaI SmlI SphI StuI StyI TaqII TatI TstI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AclI AfeI AflII AgeI AhdI AscI AseI AsiSI AvrII BaeI BbeI BclI BglI BmtI BsaBI BsaWI BsiEI BsiWI BsmBI BspEI BspHI BsrDI BssHII BstBI BstEII BstXI BstZ17I BtgZI ClaI CspCI DraI EagI EcoRI FseI FspI FspAI HindIII HpaI KasI MfeI MluI NarI NheI NotI NruI PacI PflMI PmeI PshAI PsiI PsrI PvuI RsrII SacII ScaI SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI Tth111I XbaI