Restriction map for
pFN21AB8272
(Circular) (Six-base) MAPSORT of: pFN21AB8272.seq Check: 1333 from: 1 to: 5188
pFN21AB8272 5188 bp
With 182 enzymes: SgfI *
December 8, 2011 13:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2367 2367
Size: 5188
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2998 278
Size: 53 83 186 936 1462 2468
Fragments arranged by size:
2468 1462 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2531 1937
Size: 594 4594
Acc65I G'GTAC_C
Cuts at: 2509 2509
Size: 5188
AclI AA'CG_TT
Cuts at: 1563 3315 3688 1563
Size: 1752 373 3063
Fragments arranged by size:
3063 1752 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2105 3251 4635 4903 1632
Size: 473 1146 1384 268 1917
Fragments arranged by size:
1917 1384 1146 473 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4991
AflIII A'CryG_T
Cuts at: 1203 2010 4087 5064 1203
Size: 807 2077 977 1327
Fragments arranged by size:
2077 1327 977 807
AgeI A'CCGG_T
Cuts at: 4019 4019
Size: 5188
AhdI GACnn_n'nnGTC
Cuts at: 3917 3917
Size: 5188
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5188
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5188
AlwNI CAG_nnn'CTG
Cuts at: 2134 2931 4503 2134
Size: 797 1572 2819
Fragments arranged by size:
2819 1572 797
ApaLI G'TGCA_C
Cuts at: 2354 3245 4401 2354
Size: 891 1156 3141
Fragments arranged by size:
3141 1156 891
ApoI r'AATT_y
Cuts at: 1493 2497 2723 2875 4984 1493
Size: 1004 226 152 2109 1697
Fragments arranged by size:
2109 1697 1004 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3742 160
Size: 872 2710 1606
Fragments arranged by size:
2710 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5188
AvaI C'yCGr_G
Cuts at: 1870 1943 2326 2513 1870
Size: 73 383 187 4545
Fragments arranged by size:
4545 383 187 73
BamHI G'GATC_C
Cuts at: 2518 2885 2518
Size: 367 4821
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2028 2509 3964 618
Size: 325 217 627 241 481 1455 1842
Fragments arranged by size:
1842 1455 627 481 325 241 217
BanII G_rGCy'C
Cuts at: 1391 2507 1391
Size: 1116 4072
BbeI G_GCGC'C
Cuts at: 2032 2032
Size: 5188
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4217
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2059 2093 3379 3413 1903
Size: 34 122 34 1286 34 3678
Fragments arranged by size:
3678 1286 122 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3080 4290 3080
Size: 1210 3978
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5188
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2243 3799 136
Size: 107 122 71 631 605 571 1556 1525
Fragments arranged by size:
1556 1525 631 605 571 122 107 71
BglII A'GATC_T
Cuts at: 5183 5183
Size: 5188
BlpI GC'TnA_GC
Cuts at: 2605 2605
Size: 5188
Bme1580I G_kGCm'C
Cuts at: 1790 2358 3249 4405 1790
Size: 568 891 1156 2573
Fragments arranged by size:
2573 1156 891 568
BmgBI CAC'GTC
Cuts at: 1318 2011 1318
Size: 693 4495
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2183 2919 3877
Size: 416 53 83 399 24 766 736 958
Cuts at: 3877 442
Size: 1753
Fragments arranged by size:
1753 958 766 736 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5188
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2425 3848 1239
Size: 1186 1423 2579
Fragments arranged by size:
2579 1423 1186
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2093 3313 4178 4476 2093
Size: 1220 865 298 2805
Fragments arranged by size:
2805 1220 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3851 882
Size: 617 2352 2219
Fragments arranged by size:
2352 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2979 493
Size: 711 1775 2702
Fragments arranged by size:
2702 1775 711
BsaBI GATnn'nnATC
Cuts at: 5182 5182
Size: 5188
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2029 2055 2995 3377
Size: 53 83 186 936 496 26 940 382
Cuts at: 3377 4012 275
Size: 635 1451
Fragments arranged by size:
1451 940 936 635 496 382 186 83
53 26
BsaWI w'CCGG_w
Cuts at: 3621 4019 4293 4440 4851 3621
Size: 398 274 147 411 3958
Fragments arranged by size:
3958 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2398 2428 1178
Size: 30 165 30 995 30 3938
Fragments arranged by size:
3938 995 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5188
BseYI C'CCAG_C
Cuts at: 2218 2478 4391 4887 2218
Size: 260 1913 496 2519
Fragments arranged by size:
2519 1913 496 260
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4615
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2638 3399 4427 1540
Size: 275 186 637 761 1028 2301
Fragments arranged by size:
2301 1028 761 637 275 186
BsiHKAI G_wGCw'C
Cuts at: 2358 3249 3334 4405 2358
Size: 891 85 1071 3141
Fragments arranged by size:
3141 1071 891 85
BsmI GAATG_Cn'
Cuts at: 2699 2792 2699
Size: 93 5095
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2358 2507 3249 3334 4405 1391
Size: 399 568 149 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 568 399 149 85
BspEI T'CCGG_A
Cuts at: 4851 4851
Size: 5188
BspHI T'CATG_A
Cuts at: 2019 3077 4807 2019
Size: 1058 1730 2400
Fragments arranged by size:
2400 1730 1058
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2367 2543 844
Size: 1523 176 3489
Fragments arranged by size:
3489 1523 176
BsrBI CCG'CTC
Cuts at: 1394 1823 3075 1394
Size: 429 1252 3507
Fragments arranged by size:
3507 1252 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3683 3857 65
Size: 1183 2435 174 1396
Fragments arranged by size:
2435 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3832 4019 1700
Size: 110 2022 187 2869
Fragments arranged by size:
2869 2022 187 110
BsrGI T'GTAC_A
Cuts at: 96 2136 96
Size: 2040 3148
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5188
BssSI C'ACGA_G
Cuts at: 3248 4260 3248
Size: 1012 4176
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5188
BstBI TT'CG_AA
Cuts at: 2079 3992 2079
Size: 1913 3275
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5188
BstXI CCAn_nnnn'nTGG
Cuts at: 2417 2417
Size: 5188
BstYI r'GATC_y
Cuts at: 1972 2518 2885 3270 3287 4739 4825 4837 5183
Size: 546 367 385 17 1452 86 12 346
Cuts at: 5183 1972
Size: 1977
Fragments arranged by size:
1977 1452 546 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3697
Fragments arranged by size:
3697 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4449
Fragments arranged by size:
4449 683 56
BtsI GCAGTG_nn'
Cuts at: 2341 2704 3498 3518 2341
Size: 363 794 20 4011
Fragments arranged by size:
4011 794 363 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5188
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5153
DraI TTT'AAA
Cuts at: 2492 2492
Size: 5188
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4195 809
Size: 507 2879 1802
Fragments arranged by size:
2879 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2207 2382 2635
Size: 54 1425 50 275 18 377 175 253
Cuts at: 2635 3524 5153 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 377 275 253 175 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2635 1537
Size: 275 823 4090
Fragments arranged by size:
4090 823 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3118 1353
Size: 1765 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3775 4149 4295 387
Size: 1064 138 2186 374 146 1280
Fragments arranged by size:
2186 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2105 2425 3251 3848 4635 4903 1239
Size: 393 473 320 826 597 787 268 1524
Fragments arranged by size:
1524 826 787 597 473 393 320 268
EcoO109I rG'GnC_Cy
Cuts at: 2215 2263 2215
Size: 48 5140
EcoRI G'AATT_C
Cuts at: 2497 2875 2497
Size: 378 4810
FspI TGC'GCA
Cuts at: 3694 3694
Size: 5188
HaeII r_GCGC'y
Cuts at: 1678 2032 4335 1678
Size: 354 2303 2531
Fragments arranged by size:
2531 2303 354
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2398 2430 3829 3861 3903
Size: 32 135 32 826 32 1399 32 42
Cuts at: 3903 3935 1373
Size: 32 2626
Fragments arranged by size:
2626 1399 826 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2532 2778 669
Size: 988 281 594 246 3079
Fragments arranged by size:
3079 988 594 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5188
HpaI GTT'AAC
Cuts at: 2778 2778
Size: 5188
KasI G'GCGC_C
Cuts at: 2028 2028
Size: 5188
KpnI G_GTAC'C
Cuts at: 2513 2513
Size: 5188
MfeI C'AATT_G
Cuts at: 2787 2787
Size: 5188
MluI A'CGCG_T
Cuts at: 5064 5064
Size: 5188
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4302 4486 1305
Size: 2997 184 2007
Fragments arranged by size:
2997 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2384 10
Size: 54 1425 343 552 2814
Fragments arranged by size:
2814 1425 552 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2361 3146 3505 3664 518
Size: 648 843 352 785 359 159 2042
Fragments arranged by size:
2042 843 785 648 359 352 159
MspA1I CmG'CkG
Cuts at: 1606 2131 2604 3281 4429 4674 1606
Size: 525 473 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 525 473 245
NarI GG'CG_CC
Cuts at: 2029 2029
Size: 5188
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3697
Fragments arranged by size:
3697 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5188
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5188
NotI GC'GGCC_GC
Cuts at: 2635 2635
Size: 5188
NspI r_CATG'y
Cuts at: 1132 1229 2366 2433 2546 4091 1132
Size: 97 1137 67 113 1545 2229
Fragments arranged by size:
2229 1545 1137 113 97 67
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5188
PciI A'CATG_T
Cuts at: 4087 4087
Size: 5188
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2155 2380 1108
Size: 1047 225 3916
Fragments arranged by size:
3916 1047 225
PmeI GTTT'AAAC
Cuts at: 2492 2492
Size: 5188
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3257 3289 4770 4802 3257
Size: 32 1481 32 3643
Fragments arranged by size:
3643 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5188
PsiI TTA'TAA
Cuts at: 2758 2758
Size: 5188
PstI C_TGCA'G
Cuts at: 830 2540 830
Size: 1710 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5188
SalI G'TCGA_C
Cuts at: 1936 2530 1936
Size: 594 4594
SbfI CC_TGCA'GG
Cuts at: 2540 2540
Size: 5188
ScaI AGT'ACT
Cuts at: 3436 5148 3436
Size: 1712 3476
SfcI C'TryA_G
Cuts at: 826 1045 2536 3671 4352 4543 826
Size: 219 1491 1135 681 191 1471
Fragments arranged by size:
1491 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2030 2030
Size: 5188
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5188
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5188
SmaI CCC'GGG
Cuts at: 1872 2515 1872
Size: 643 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2108 3292 4193 4455 820
Size: 197 926 165 1184 901 262 1553
Fragments arranged by size:
1553 1184 926 901 262 197 165
SnaBI TAC'GTA
Cuts at: 493 2979 493
Size: 2486 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5188
SphI G_CATG'C
Cuts at: 1132 2546 1132
Size: 1414 3774
SspI AAT'ATT
Cuts at: 5 52 3112 5
Size: 47 3060 2081
Fragments arranged by size:
3060 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5188
StyI C'CwwG_G
Cuts at: 513 1065 2004 2058 2627 513
Size: 552 939 54 569 3074
Fragments arranged by size:
3074 939 569 552 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2487 3215 3232 3385 3570 4015 325
Size: 1255 907 728 17 153 185 445 1498
Fragments arranged by size:
1498 1255 907 728 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2136 3434 5146
Size: 274 80 33 51 1443 159 1298 1712
Cuts at: 5146 96
Size: 138
Fragments arranged by size:
1712 1443 1298 274 159 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5148 5180 5148
Size: 32 5156
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5188
XbaI T'CTAG_A
Cuts at: 2524 2524
Size: 5188
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2230 2230
Size: 5188
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5188
XmaI C'CCGG_G
Cuts at: 1870 2513 1870
Size: 643 4545
XmnI GAAnn'nnTTC
Cuts at: 3317 3317
Size: 5188
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2996 276
Size: 53 83 186 936 1462 2468
Fragments arranged by size:
2468 1462 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SapI SexAI SfiI
SrfI SwaI