Restriction map for
pFN21AE2450
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# NO CUT ENZYMES ( 136 enzymes) #
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AatII AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI AluI
AlwNI AocI Aor51HI ApaI ApoI
AscI AseI AsnI Asp700I AspEI
AspI AviII AvrII BamHI BbrPI
BbvCI BcgI BciVI BfrI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaI BsaWI BseAI BsiEI BsiWI
BsmAI BsmBI BsmI BspDI BspEI
BspHI BspLU11I BsrBI BsrFI BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoT14I EcoT22I FseI FspI
HincII HindII HindIII HpaI MaeII
MamI MfeI MluI MroI MseI
MunI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SalI SapI SbfI SexAI
SfiI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspI StuI
StyI SwaI TaqI Tru9I Tsp509I
Tth111I TthHB8I Van91I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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Acc65I 324 GGTACC G^GTACC
AcyI 579 GGCGCC G[GA]^CG[CT]C
Alw44I 640 GTGCAC G^TGCAC
ApaLI 640 GTGCAC G^TGCAC
Asp718I 324 GGTACC G^GTACC
BanII 100 GAGCCC G[GA]GC[CT]^C
BbeI 579 GGCGCC GGCGC^C
BclI 359 TGATCA T^GATCA
BfaI 49 CTAG C^TAG
BglI 629 GCCTGGACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 766 CTGGAG CTGGAG(16/14)
BsaHI 579 GGCGCC G[GA]^CG[CT]C
Bsp1407I 611 TGTACA T^GTACA
BsrDI 783 CATTGC GCAATG(2/0)
BsrGI 611 TGTACA T^GTACA
BssSI 289 CTCGTG CACGAG(-5/-1)
BstYI 761 AGATCC [GA]^GATC[CT]
DdeI 296 CTGAG C^T[ACGT]AG
EarI 492 GAAGAG CTCTTC(1/4)
EcoNI 765 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 13 GATATC GAT^ATC
FbaI 359 TGATCA T^GATCA
HgaI 144 GACGC GACGC(5/10)
Hin1I 579 GGCGCC G[GA]^CG[CT]C
KasI 579 GGCGCC G^GCGCC
KpnI 324 GGTACC GGTAC^C
Ksp632I 492 GAAGAG CTCTTC(1/4)
KspI 624 CCGCGG CCGC^GG
MaeI 49 CTAG C^TAG
MflI 761 AGATCC [GA]^GATC[CT]
MslI 912 CATCCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 579 GGCGCC GG^CGCC
PshAI 447 GACCAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 624 CCGCGG CCGC^GG
ScaI 341 AGTACT AGT^ACT
SfoI 579 GGCGCC GGC^GCC
SmaI 499 CCCGGG CCC^GGG
SspBI 611 TGTACA T^GTACA
XcmI 356 CCATGATCATCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 761 AGATCC [GA]^GATC[CT]
XmaCI 499 CCCGGG C^CCGGG
XmaI 499 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccII 474 CGCG CG^CG
625 CGCG
AlwI 171 GATCC GGATC(4/5)
762 GATCC
AvaI 499 CCCGGG C^[CT]CG[GA]G
736 CCCGAG
BalI 554 TGGCCA TGG^CCA
755 TGGCCA
BanI 324 GGTACC G^G[CT][GA]CC
579 GGCGCC
BbsI 277 GTCTTC GAAGAC(2/6)
886 GTCTTC
BpuAI 277 GTCTTC GAAGAC(2/6)
886 GTCTTC
BseRI 69 GAGGAG GAGGAG(10/8)
769 GAGGAG
BsgI 718 CTGCAC GTGCAG(16/14)
900 CTGCAC
BsoBI 499 CCCGGG C^[CT]CG[GA]G
736 CCCGAG
BspMI 164 ACCTGC ACCTGC(4/8)
605 ACCTGC
BstAPI 683 GCATGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
711 GCACCTCCTGC
BstUI 474 CGCG CG^CG
625 CGCG
BstXI 758 CCAAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
929 CCAACTTCGTGG
DraII 44 GGGACCT [GA]G^G[ACGT]CC[CT]
743 GGGACCC
DsaI 575 CCGTGG C^C[GA][CT]GG
624 CCGCGG
EcoO109I 44 GGGACCT [GA]G^G[ACGT]CC[CT]
743 GGGACCC
FauI 307 CCCGC CCCGC(4/6)
589 CCCGC
MluNI 554 TGGCCA TGG^CCA
755 TGGCCA
MscI 554 TGGCCA TGG^CCA
755 TGGCCA
MvnI 474 CGCG CG^CG
625 CGCG
PleI 661 GACTC GAGTC(4/5)
452 GAGTC
PpuMI 44 GGGACCT [GA]G^G[AT]CC[CT]
743 GGGACCC
SfaNI 435 GATGC GCATC(5/9)
911 GCATC
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# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AciI AfaI AspHI AvaII BbvI
BcnI BmyI BsaJI BsiHKAI BsiYI
BslI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DpnII EaeI EcoRII Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeIII
MboI MboII MnlI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PstI RsaI Sau3AI Sau96I
ScrFI SfcI TfiI TseI Tsp45I
TspRI