################################## # NO CUT ENZYMES ( 117 enzymes) # ################################## AatII AccI AccIII AclI AflII AgeI AhdI AocI ApaI AscI AseI AsnI Asp700I AspEI AspI AviII AvrII BamHI BbrPI BbvCI BcgI BciVI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BsiEI BsiWI BsmAI BsmBI BsmI BspEI BspHI BspLU11I BsrBI BsrFI BssHII Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I CelII Cfr10I CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco52I Eco81I EcoO65I EcoT22I FseI FspI HincII HindII HindIII HpaI MamI MluI MroI MseI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SalI SapI SbfI SexAI SfiI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I StuI SwaI Tru9I Tth111I Van91I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### Acc65I 597 GGTACC G^GTACC AcsI 126 GAATTC [GA]^AATT[CT] AflIII 116 ACGTGT A^C[GA][CT]GT AluI 63 AGCT AG^CT Alw44I 913 GTGCAC G^TGCAC AlwNI 145 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 89 AGCGCT AGC^GCT ApaLI 913 GTGCAC G^TGCAC ApoI 126 GAATTC [GA]^AATT[CT] Asp718I 597 GGTACC G^GTACC BanII 373 GAGCCC G[GA]GC[CT]^C BbeI 852 GGCGCC GGCGC^C BclI 632 TGATCA T^GATCA BfaI 322 CTAG C^TAG BglI 902 GCCTGGACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC Bsp1407I 884 TGTACA T^GTACA BspDI 184 ATCGAT AT^CGAT BsrGI 884 TGTACA T^GTACA BssSI 562 CTCGTG CACGAG(-5/-1) ClaI 184 ATCGAT AT^CGAT DdeI 569 CTGAG C^T[ACGT]AG EarI 765 GAAGAG CTCTTC(1/4) Eco47III 89 AGCGCT AGC^GCT EcoNI 1038 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 126 GAATTC G^AATTC EcoRV 286 GATATC GAT^ATC EcoT14I 260 CCTTGG C^C[AT][AT]GG FbaI 632 TGATCA T^GATCA HgaI 97 GACGC GACGC(5/10) KasI 852 GGCGCC G^GCGCC KpnI 597 GGTACC GGTAC^C Ksp632I 765 GAAGAG CTCTTC(1/4) KspI 897 CCGCGG CCGC^GG MaeI 322 CTAG C^TAG MaeII 116 ACGT A^CGT MfeI 37 CAATTG C^AATTG MunI 37 CAATTG C^AATTG NarI 852 GGCGCC GG^CGCC PshAI 720 GACCAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacII 897 CCGCGG CCGC^GG ScaI 614 AGTACT AGT^ACT SfoI 852 GGCGCC GGC^GCC SmaI 772 CCCGGG CCC^GGG SspBI 884 TGTACA T^GTACA SspI 8 AATATT AAT^ATT StyI 260 CCTTGG C^C[AT][AT]GG TaqI 185 TCGA T^CGA TthHB8I 185 TCGA T^CGA XcmI 629 CCATGATCATCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 772 CCCGGG C^CCGGG XmaI 772 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AccII 747 CGCG CG^CG 898 CGCG AcyI 97 GACGCC G[GA]^CG[CT]C 852 GGCGCC AvaI 772 CCCGGG C^[CT]CG[GA]G 1009 CCCGAG BalI 827 TGGCCA TGG^CCA 1028 TGGCCA BanI 597 GGTACC G^G[CT][GA]CC 852 GGCGCC BbsI 550 GTCTTC GAAGAC(2/6) 1159 GTCTTC BpmI 155 CTCCAG CTGGAG(16/14) 1039 CTGGAG BpuAI 550 GTCTTC GAAGAC(2/6) 1159 GTCTTC BsaHI 97 GACGCC G[GA]^CG[CT]C 852 GGCGCC BseRI 342 GAGGAG GAGGAG(10/8) 1042 GAGGAG BsoBI 772 CCCGGG C^[CT]CG[GA]G 1009 CCCGAG BsrDI 139 CATTGC GCAATG(2/0) 1056 CATTGC BstAPI 956 GCATGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 984 GCACCTCCTGC BstUI 747 CGCG CG^CG 898 CGCG BstXI 1031 CCAAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1202 CCAACTTCGTGG BstYI 264 GGATCT [GA]^GATC[CT] 1034 AGATCC DsaI 848 CCGTGG C^C[GA][CT]GG 897 CCGCGG FauI 580 CCCGC CCCGC(4/6) 862 CCCGC Hin1I 97 GACGCC G[GA]^CG[CT]C 852 GGCGCC MflI 264 GGATCT [GA]^GATC[CT] 1034 AGATCC MluNI 827 TGGCCA TGG^CCA 1028 TGGCCA MscI 827 TGGCCA TGG^CCA 1028 TGGCCA MslI 111 CACTAACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1185 CATCCTGATG MvnI 747 CGCG CG^CG 898 CGCG PleI 934 GACTC GAGTC(4/5) 725 GAGTC Tsp509I 38 AATT ^AATT 127 AATT XhoII 264 GGATCT [GA]^GATC[CT] 1034 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 63 enzymes) # ######################################### AciI AfaI AlwI AspHI AvaII BbvI BcnI BmyI BsaJI BsgI BsiHKAI BsiYI BslI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DpnII DraII EaeI EcoO109I EcoRII Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeIII MboI MboII MnlI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TfiI TseI Tsp45I TspRI