(Circular) (Six-base) MAPSORT of: pFN21AE5562.seq Check: 4921 from: 1 to: 5941 pFN21AE5562 5941 bp With 182 enzymes: SgfI * January 30, 2014 17:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3751 278 Size: 53 83 186 936 2215 2468 Fragments arranged by size: 2468 2215 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3284 1937 Size: 1347 4594 Acc65I G'GTAC_C Cuts at: 2602 3262 2602 Size: 660 5281 AclI AA'CG_TT Cuts at: 1563 4068 4441 1563 Size: 2505 373 3063 Fragments arranged by size: 3063 2505 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2252 4004 5388 5656 1632 Size: 620 1752 1384 268 1917 Fragments arranged by size: 1917 1752 1384 620 268 AfeI AGC'GCT Cuts at: 2096 2096 Size: 5941 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5744 AflIII A'CryG_T Cuts at: 1203 2121 4840 5817 1203 Size: 918 2719 977 1327 Fragments arranged by size: 2719 1327 977 918 AgeI A'CCGG_T Cuts at: 4772 4772 Size: 5941 AhdI GACnn_n'nnGTC Cuts at: 4670 4670 Size: 5941 AleI CACnn'nnGTG Cuts at: 1166 2120 1166 Size: 954 4987 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5941 AlwNI CAG_nnn'CTG Cuts at: 2155 3684 5256 2155 Size: 1529 1572 2840 Fragments arranged by size: 2840 1572 1529 ApaLI G'TGCA_C Cuts at: 2918 3998 5154 2918 Size: 1080 1156 3705 Fragments arranged by size: 3705 1156 1080 ApoI r'AATT_y Cuts at: 1493 2131 3250 3476 3628 5737 1493 Size: 638 1119 226 152 2109 1697 Fragments arranged by size: 2109 1697 1119 638 226 152 AseI AT'TA_AT Cuts at: 160 1032 4495 160 Size: 872 3463 1606 Fragments arranged by size: 3463 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5941 AvaI C'yCGr_G Cuts at: 1870 1943 2777 3014 3266 1870 Size: 73 834 237 252 4545 Fragments arranged by size: 4545 834 252 237 73 BamHI G'GATC_C Cuts at: 3271 3638 3271 Size: 367 5574 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2602 2857 3262 4717 618 Size: 325 217 627 815 255 405 1455 1842 Fragments arranged by size: 1842 1455 815 627 405 325 255 217 BanII G_rGCy'C Cuts at: 1391 2382 3260 1391 Size: 991 878 4072 Fragments arranged by size: 4072 991 878 BbeI G_GCGC'C Cuts at: 2861 2861 Size: 5941 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2548 3157 928 Size: 971 649 609 3712 Fragments arranged by size: 3712 971 649 609 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4132 4166 1903 Size: 34 50 34 2111 34 3678 Fragments arranged by size: 3678 2111 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3833 5043 3833 Size: 1210 4731 BclI T'GATC_A Cuts at: 1548 2637 1548 Size: 1089 4852 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2913 4552 136 Size: 107 122 71 631 605 1241 1639 1525 Fragments arranged by size: 1639 1525 1241 631 605 122 107 71 BglII A'GATC_T Cuts at: 5936 5936 Size: 5941 BlpI GC'TnA_GC Cuts at: 3358 3358 Size: 5941 Bme1580I G_kGCm'C Cuts at: 1790 2922 4002 5158 1790 Size: 1132 1080 1156 2573 Fragments arranged by size: 2573 1156 1132 1080 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5941 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2611 3672 4630 Size: 416 53 83 399 24 1194 1061 958 Cuts at: 4630 442 Size: 1753 Fragments arranged by size: 1753 1194 1061 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5941 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2145 3065 4601 1239 Size: 906 920 1536 2579 Fragments arranged by size: 2579 1536 920 906 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4066 4931 5229 4066 Size: 865 298 4778 Fragments arranged by size: 4778 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4604 882 Size: 617 3105 2219 Fragments arranged by size: 3105 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3732 493 Size: 711 2528 2702 Fragments arranged by size: 2702 2528 711 BsaBI GATnn'nnATC Cuts at: 5935 5935 Size: 5941 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2103 2858 3748 4130 Size: 53 83 186 936 570 755 890 382 Cuts at: 4130 4765 275 Size: 635 1451 Fragments arranged by size: 1451 936 890 755 635 570 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4374 4772 5046 5193 5604 4374 Size: 398 274 147 411 4711 Fragments arranged by size: 4711 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3209 3239 1178 Size: 30 165 30 1806 30 3880 Fragments arranged by size: 3880 1806 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2362 3062 1186 Size: 1176 700 4065 Fragments arranged by size: 4065 1176 700 BseYI C'CCAG_C Cuts at: 5144 5640 5144 Size: 496 5445 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2237 2981 3163 1163 Size: 573 501 744 182 3941 Fragments arranged by size: 3941 744 573 501 182 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3391 4152 5180 1540 Size: 275 186 1390 761 1028 2301 Fragments arranged by size: 2301 1390 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2463 2922 2991 4002 4087 5158 2463 Size: 459 69 1011 85 1071 3246 Fragments arranged by size: 3246 1071 1011 459 85 69 BsmI GAATG_Cn' Cuts at: 3452 3545 3452 Size: 93 5848 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2382 2463 2922 2991 3260 4002 4087 Size: 399 592 81 459 69 269 742 85 Cuts at: 4087 5158 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 592 459 399 269 85 81 69 BspEI T'CCGG_A Cuts at: 5604 5604 Size: 5941 BspHI T'CATG_A Cuts at: 3830 5560 3830 Size: 1730 4211 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2163 2451 2892 3296 844 Size: 1319 288 441 404 3489 Fragments arranged by size: 3489 1319 441 404 288 BsrBI CCG'CTC Cuts at: 1394 1823 3828 1394 Size: 429 2005 3507 Fragments arranged by size: 3507 2005 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2143 3060 4436 4610 65 Size: 1183 895 917 1376 174 1396 Fragments arranged by size: 1396 1376 1183 917 895 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4585 4772 1700 Size: 110 2775 187 2869 Fragments arranged by size: 2869 2775 187 110 BsrGI T'GTAC_A Cuts at: 96 2889 96 Size: 2793 3148 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5941 BssSI C'ACGA_G Cuts at: 2567 4001 5013 2567 Size: 1434 1012 3495 Fragments arranged by size: 3495 1434 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2967 2995 794 Size: 2173 28 3740 Fragments arranged by size: 3740 2173 28 BstBI TT'CG_AA Cuts at: 4745 4745 Size: 5941 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5941 BstXI CCAn_nnnn'nTGG Cuts at: 3043 3214 3043 Size: 171 5770 BstYI r'GATC_y Cuts at: 1972 2269 3039 3271 3638 4023 4040 5492 5578 Size: 297 770 232 367 385 17 1452 86 Cuts at: 5578 5590 5936 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 770 385 367 346 297 232 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2853 2902 513 Size: 552 1788 49 3552 Fragments arranged by size: 3552 1788 552 49 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5202 Fragments arranged by size: 5202 683 56 BtsI GCAGTG_nn' Cuts at: 2221 3457 4251 4271 2221 Size: 1236 794 20 3891 Fragments arranged by size: 3891 1236 794 20 ClaI AT'CG_AT Cuts at: 1554 2190 1554 Size: 636 5305 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5906 DraI TTT'AAA Cuts at: 3245 3245 Size: 5941 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4948 809 Size: 507 3632 1802 Fragments arranged by size: 3632 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2832 2956 3033 Size: 54 1425 50 275 18 1002 124 77 Cuts at: 3033 3388 4277 5906 8 Size: 355 889 1629 43 Fragments arranged by size: 1629 1425 1002 889 355 275 124 77 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3388 1537 Size: 275 1576 4090 Fragments arranged by size: 4090 1576 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2765 3871 1353 Size: 1412 1106 3423 Fragments arranged by size: 3423 1412 1106 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2458 4528 4902 5048 387 Size: 1064 138 869 2070 374 146 1280 Fragments arranged by size: 2070 1280 1064 869 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2145 2252 3065 4004 4601 5388 5656 Size: 393 513 107 813 939 597 787 268 Cuts at: 5656 1239 Size: 1524 Fragments arranged by size: 1524 939 813 787 597 513 393 268 107 EcoNI CCTnn'n_nnAGG Cuts at: 3047 3047 Size: 5941 EcoO109I rG'GnC_Cy Cuts at: 2241 2323 3022 2241 Size: 82 699 5160 Fragments arranged by size: 5160 699 82 EcoRI G'AATT_C Cuts at: 2131 3250 3628 2131 Size: 1119 378 4444 Fragments arranged by size: 4444 1119 378 EcoRV GAT'ATC Cuts at: 2293 2293 Size: 5941 FspI TGC'GCA Cuts at: 4447 4447 Size: 5941 HaeII r_GCGC'y Cuts at: 1678 2098 2824 2853 2861 5088 1678 Size: 420 726 29 8 2227 2531 Fragments arranged by size: 2531 2227 726 420 29 8 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3187 3209 3219 3241 4582 Size: 32 135 32 1615 22 10 22 1341 Cuts at: 4582 4614 4656 4688 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1615 1341 135 42 32 32 32 32 22 22 10 HincII GTy'rAC Cuts at: 669 1657 1938 3285 3531 669 Size: 988 281 1347 246 3079 Fragments arranged by size: 3079 1347 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5941 HpaI GTT'AAC Cuts at: 3531 3531 Size: 5941 KasI G'GCGC_C Cuts at: 2857 2857 Size: 5941 KpnI G_GTAC'C Cuts at: 2606 3266 2606 Size: 660 5281 MfeI C'AATT_G Cuts at: 2042 3540 2042 Size: 1498 4443 MluI A'CGCG_T Cuts at: 5817 5817 Size: 5941 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5055 5239 1305 Size: 3750 184 2007 Fragments arranged by size: 3750 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2834 3035 10 Size: 54 1425 343 1002 201 2916 Fragments arranged by size: 2916 1425 1002 343 201 54 MslI CAynn'nnrTG Cuts at: 518 1166 2120 3194 3899 4258 4417 518 Size: 648 954 1074 705 359 159 2042 Fragments arranged by size: 2042 1074 954 705 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2818 2904 3078 3357 4034 5182 5427 1606 Size: 1212 86 174 279 677 1148 245 2120 Fragments arranged by size: 2120 1212 1148 677 279 245 174 86 NarI GG'CG_CC Cuts at: 2858 2858 Size: 5941 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5389 NdeI CA'TA_TG Cuts at: 387 387 Size: 5941 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5941 NotI GC'GGCC_GC Cuts at: 3388 3388 Size: 5941 NspI r_CATG'y Cuts at: 1132 1229 3299 4844 1132 Size: 97 2070 1545 2229 Fragments arranged by size: 2229 2070 1545 97 PasI CC'CwG_GG Cuts at: 1348 2524 1348 Size: 1176 4765 PciI A'CATG_T Cuts at: 4840 4840 Size: 5941 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5941 PfoI T'CCnGG_A Cuts at: 3042 3042 Size: 5941 PmeI GTTT'AAAC Cuts at: 3245 3245 Size: 5941 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3101 3133 4010 4042 5523 5555 3101 Size: 32 877 32 1481 32 3487 Fragments arranged by size: 3487 1481 877 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2241 2323 3022 2241 Size: 82 699 5160 Fragments arranged by size: 5160 699 82 PshAI GACnn'nnGTC Cuts at: 1613 2729 1613 Size: 1116 4825 PsiI TTA'TAA Cuts at: 3511 3511 Size: 5941 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2873 2905 2873 Size: 32 5909 PstI C_TGCA'G Cuts at: 830 2277 2503 2682 2828 3107 3293 830 Size: 1447 226 179 146 279 186 3478 Fragments arranged by size: 3478 1447 279 226 186 179 146 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5941 SacII CC_GC'GG Cuts at: 2905 2905 Size: 5941 SalI G'TCGA_C Cuts at: 1936 3283 1936 Size: 1347 4594 SanDI GG'GwC_CC Cuts at: 3022 3022 Size: 5941 SbfI CC_TGCA'GG Cuts at: 3293 3293 Size: 5941 ScaI AGT'ACT Cuts at: 2621 4189 5901 2621 Size: 1568 1712 2661 Fragments arranged by size: 2661 1712 1568 SfcI C'TryA_G Cuts at: 826 1045 2273 2499 2678 2824 3103 3289 4424 Size: 219 1228 226 179 146 279 186 1135 Cuts at: 4424 5105 5296 826 Size: 681 191 1471 Fragments arranged by size: 1471 1228 1135 681 279 226 219 191 186 179 146 SfoI GGC'GCC Cuts at: 2859 2859 Size: 5941 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5941 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5941 SmaI CCC'GGG Cuts at: 1872 2779 3268 1872 Size: 907 489 4545 Fragments arranged by size: 4545 907 489 SmlI C'TyrA_G Cuts at: 820 1017 1943 4045 4946 5208 820 Size: 197 926 2102 901 262 1553 Fragments arranged by size: 2102 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3732 493 Size: 3239 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5941 SphI G_CATG'C Cuts at: 1132 3299 1132 Size: 2167 3774 SspI AAT'ATT Cuts at: 5 52 2015 3865 5 Size: 47 1963 1850 2081 Fragments arranged by size: 2081 1963 1850 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5941 StyI C'CwwG_G Cuts at: 513 1065 2265 3380 513 Size: 552 1200 1115 3074 Fragments arranged by size: 3074 1200 1115 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3151 3968 3985 4138 4323 4768 325 Size: 1255 1571 817 17 153 185 445 1498 Fragments arranged by size: 1571 1498 1255 817 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2619 2889 4187 Size: 274 80 33 51 1443 642 270 1298 Cuts at: 4187 5899 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1298 642 274 270 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3128 3160 5901 5933 3128 Size: 32 2741 32 3136 Fragments arranged by size: 3136 2741 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5941 XbaI T'CTAG_A Cuts at: 3277 3277 Size: 5941 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2641 2641 Size: 5941 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5941 XmaI C'CCGG_G Cuts at: 1870 2777 3266 1870 Size: 907 489 4545 Fragments arranged by size: 4545 907 489 XmnI GAAnn'nnTTC Cuts at: 4070 4070 Size: 5941 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3749 276 Size: 53 83 186 936 2215 2468 Fragments arranged by size: 2468 2215 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PvuII RsrII SacI SapI SexAI SfiI SrfI SwaI