(Linear) (Six-base) MAPSORT of: KIEE5562.seq Check: 7176 from: 1 to: 1236 KIEE5562.orf With 182 enzymes: SgfI * January 29, 2014 12:05 .. Acc65I G'GTAC_C Cuts at: 0 597 1236 Size: 597 639 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 247 1236 Size: 247 989 AfeI AGC'GCT Cuts at: 0 91 1236 Size: 91 1145 AflIII A'CryG_T Cuts at: 0 116 1236 Size: 116 1120 AleI CACnn'nnGTG Cuts at: 0 115 1236 Size: 115 1121 AlwNI CAG_nnn'CTG Cuts at: 0 150 1236 Size: 150 1086 ApaLI G'TGCA_C Cuts at: 0 913 1236 Size: 913 323 ApoI r'AATT_y Cuts at: 0 126 1236 Size: 126 1110 AvaI C'yCGr_G Cuts at: 0 772 1009 1236 Size: 772 237 227 Fragments arranged by size: 772 237 227 BanI G'GyrC_C Cuts at: 0 597 852 1236 Size: 597 255 384 Fragments arranged by size: 597 384 255 BanII G_rGCy'C Cuts at: 0 377 1236 Size: 377 859 BbeI G_GCGC'C Cuts at: 0 856 1236 Size: 856 380 BbsI GAAGACnn'nnnn_ Cuts at: 0 543 1152 1236 Size: 543 609 84 Fragments arranged by size: 609 543 84 BclI T'GATC_A Cuts at: 0 632 1236 Size: 632 604 BglI GCCn_nnn'nGGC Cuts at: 0 908 1236 Size: 908 328 Bme1580I G_kGCm'C Cuts at: 0 917 1236 Size: 917 319 BmrI ACTGGGnnnn_n' Cuts at: 0 606 1236 Size: 606 630 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 140 1060 1236 Size: 140 920 176 Fragments arranged by size: 920 176 140 BsaHI Gr'CG_yC Cuts at: 0 98 853 1236 Size: 98 755 383 Fragments arranged by size: 755 383 98 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1204 1234 1236 Size: 1204 30 2 Fragments arranged by size: 1204 30 2 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 357 1057 1236 Size: 357 700 179 Fragments arranged by size: 700 357 179 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 232 976 1158 1236 Size: 232 744 182 78 Fragments arranged by size: 744 232 182 78 BsiHKAI G_wGCw'C Cuts at: 0 458 917 986 1236 Size: 458 459 69 250 Fragments arranged by size: 459 458 250 69 Bsp1286I G_dGCh'C Cuts at: 0 377 458 917 986 1236 Size: 377 81 459 69 250 Fragments arranged by size: 459 377 250 81 69 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 158 446 887 1236 Size: 158 288 441 349 Fragments arranged by size: 441 349 288 158 BsrDI GCAATG_nn' Cuts at: 0 138 1055 1236 Size: 138 917 181 Fragments arranged by size: 917 181 138 BsrGI T'GTAC_A Cuts at: 0 884 1236 Size: 884 352 BssSI C'ACGA_G Cuts at: 0 562 1236 Size: 562 674 BstAPI GCAn_nnn'nTGC Cuts at: 0 962 990 1236 Size: 962 28 246 Fragments arranged by size: 962 246 28 BstXI CCAn_nnnn'nTGG Cuts at: 0 1038 1209 1236 Size: 1038 171 27 Fragments arranged by size: 1038 171 27 BstYI r'GATC_y Cuts at: 0 264 1034 1236 Size: 264 770 202 Fragments arranged by size: 770 264 202 BtgI C'CryG_G Cuts at: 0 848 897 1236 Size: 848 49 339 Fragments arranged by size: 848 339 49 BtsI GCAGTG_nn' Cuts at: 0 216 1236 Size: 216 1020 ClaI AT'CG_AT Cuts at: 0 185 1236 Size: 185 1051 EaeI y'GGCC_r Cuts at: 0 827 951 1028 1236 Size: 827 124 77 208 Fragments arranged by size: 827 208 124 77 EarI CTCTTCn'nnn_ Cuts at: 0 760 1236 Size: 760 476 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 453 1236 Size: 453 783 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 140 247 1060 1236 Size: 140 107 813 176 Fragments arranged by size: 813 176 140 107 EcoNI CCTnn'n_nnAGG Cuts at: 0 1042 1236 Size: 1042 194 EcoO109I rG'GnC_Cy Cuts at: 0 236 318 1017 1236 Size: 236 82 699 219 Fragments arranged by size: 699 236 219 82 EcoRI G'AATT_C Cuts at: 0 126 1236 Size: 126 1110 EcoRV GAT'ATC Cuts at: 0 288 1236 Size: 288 948 HaeII r_GCGC'y Cuts at: 0 93 819 848 856 1236 Size: 93 726 29 8 380 Fragments arranged by size: 726 380 93 29 8 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1182 1204 1214 1236 Size: 1182 22 10 22 Fragments arranged by size: 1182 22 22 10 KasI G'GCGC_C Cuts at: 0 852 1236 Size: 852 384 KpnI G_GTAC'C Cuts at: 0 601 1236 Size: 601 635 MfeI C'AATT_G Cuts at: 0 37 1236 Size: 37 1199 MscI TGG'CCA Cuts at: 0 829 1030 1236 Size: 829 201 206 Fragments arranged by size: 829 206 201 MslI CAynn'nnrTG Cuts at: 0 115 1189 1236 Size: 115 1074 47 Fragments arranged by size: 1074 115 47 MspA1I CmG'CkG Cuts at: 0 813 899 1073 1236 Size: 813 86 174 163 Fragments arranged by size: 813 174 163 86 NarI GG'CG_CC Cuts at: 0 853 1236 Size: 853 383 PasI CC'CwG_GG Cuts at: 0 519 1236 Size: 519 717 PfoI T'CCnGG_A Cuts at: 0 1037 1236 Size: 1037 199 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1096 1128 1236 Size: 1096 32 108 Fragments arranged by size: 1096 108 32 PpuMI rG'GwC_Cy Cuts at: 0 236 318 1017 1236 Size: 236 82 699 219 Fragments arranged by size: 699 236 219 82 PshAI GACnn'nnGTC Cuts at: 0 724 1236 Size: 724 512 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 868 900 1236 Size: 868 32 336 Fragments arranged by size: 868 336 32 PstI C_TGCA'G Cuts at: 0 272 498 677 823 1102 1236 Size: 272 226 179 146 279 134 Fragments arranged by size: 279 272 226 179 146 134 SacII CC_GC'GG Cuts at: 0 900 1236 Size: 900 336 SanDI GG'GwC_CC Cuts at: 0 1017 1236 Size: 1017 219 ScaI AGT'ACT Cuts at: 0 616 1236 Size: 616 620 SfcI C'TryA_G Cuts at: 0 268 494 673 819 1098 1236 Size: 268 226 179 146 279 138 Fragments arranged by size: 279 268 226 179 146 138 SfoI GGC'GCC Cuts at: 0 854 1236 Size: 854 382 SmaI CCC'GGG Cuts at: 0 774 1236 Size: 774 462 SspI AAT'ATT Cuts at: 0 10 1236 Size: 10 1226 StyI C'CwwG_G Cuts at: 0 260 1236 Size: 260 976 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1146 1236 Size: 1146 90 TatI w'GTAC_w Cuts at: 0 614 884 1236 Size: 614 270 352 Fragments arranged by size: 614 352 270 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1123 1155 1236 Size: 1123 32 81 Fragments arranged by size: 1123 81 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 636 1236 Size: 636 600 XmaI C'CCGG_G Cuts at: 0 772 1236 Size: 772 464 Enzymes that do cut: Acc65I AcuI AfeI AflIII AleI AlwNI ApaLI ApoI AvaI BanI BanII BbeI BbsI BclI BglI Bme1580I BmrI BpmI BsaHI BsaXI BseRI BsgI BsiHKAI Bsp1286I BspMI BsrDI BsrGI BssSI BstAPI BstXI BstYI BtgI BtsI ClaI EaeI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV HaeII Hin4I KasI KpnI MfeI MscI MslI MspA1I NarI PasI PfoI PpiI PpuMI PshAI PsrI PstI SacII SanDI ScaI SfcI SfoI SmaI SspI StyI TaqII TatI TstI XcmI XmaI Enzymes that do not cut: AarI AatII AccI AclI AflII AgeI AhdI AlfI AloI ApaI AscI AseI AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI BfrBI BglII BlpI BmgBI BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BseYI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrFI BssHII BstBI BstEII BstZ17I Bsu36I BtgZI CspCI DraI DraIII DrdI EagI EcoICRI FalI FseI FspI FspAI HincII HindIII HpaI MluI MmeI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PflMI PmeI PmlI PsiI PspOMI PspXI PvuI PvuII RsrII SacI SalI SapI SbfI SexAI SfiI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI StuI SwaI Tth111I XbaI XhoI XmnI ZraI