################################## # NO CUT ENZYMES ( 137 enzymes) # ################################## AatII AccI AccII AccIII AclI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AspEI AspI AvaI AviII AvrII BalI BbrPI BbvCI BcgI BclI BfrI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaI BseAI BsiEI BsiWI BsmAI BsmBI BsmFI BsoBI BspDI BspEI BspHI BspLU11I BsrFI BssHII BssSI Bst1107I BstBI BstEII BstF5I BstPI BstUI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRV EcoT22I FbaI FokI FseI FspI HincII HindII HpaI KspI MaeIII MamI MfeI MluI MluNI MroI MscI MspA1I MunI MvnI NaeI NcoI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SbfI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I StuI SwaI TaqI Tsp45I Tth111I TthHB8I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### Acc65I 1176 GGTACC G^GTACC AcyI 3 GGCGCC G[GA]^CG[CT]C AlwNI 1272 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG Asp718I 1176 GGTACC G^GTACC AspHI 247 GAGCAC G[AT]GC[AT]^C BamHI 1104 GGATCC G^GATCC BanII 736 GAGCCC G[GA]GC[CT]^C BbeI 3 GGCGCC GGCGC^C BciVI 1413 GTATCC GTATCC(6/5) BcnI 24 CCGGG CC^[GC]GG BglI 6 GCCGGAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaHI 3 GGCGCC G[GA]^CG[CT]C BsaWI 447 TCCGGT [AT]^CCGG[AT] BsiHKAI 247 GAGCAC G[AT]GC[AT]^C BspMI 949 ACCTGC ACCTGC(4/8) BsrBI 10 GAGCGG CCGCTC(-3/-3) BsrDI 1437 CATTGC GCAATG(2/0) BstAPI 1271 GCAGAAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 1378 CCAGTGTATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DsaI 63 CCGTGG C^C[GA][CT]GG FauI 48 GCGGG CCCGC(4/6) HaeIII 1133 GGCC GG^CC HgaI 1280 GCGTC GACGC(5/10) Hin1I 3 GGCGCC G[GA]^CG[CT]C HindIII 1007 AAGCTT A^AGCTT KasI 3 GGCGCC G^GCGCC KpnI 1176 GGTACC GGTAC^C MaeII 251 ACGT A^CGT NarI 3 GGCGCC GG^CGCC NciI 24 CCGGG CC^[GC]GG NspI 661 GCATGC [GA]CATG^[CT] PflMI 1507 CCAAGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 1353 GACTC GAGTC(4/5) SapI 94 GCTCTTC GCTCTTC(1/4) SfcI 242 CTATAG C^T[GA][CT]AG SfoI 3 GGCGCC GGC^GCC SphI 661 GCATGC GCATG^C SspI 769 AATATT AAT^ATT TfiI 1018 GATTC G^A[AT]TC Van91I 1507 CCAAGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AseI 145 ATTAAT AT^TAAT 772 ATTAAT AsnI 145 ATTAAT AT^TAAT 772 ATTAAT Asp700I 91 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 904 GAAAAACTTC BanI 3 GGCGCC G^G[CT][GA]CC 1176 GGTACC BseRI 443 CTCCTC GAGGAG(10/8) 838 GAGGAG BsmI 217 GAATGC GAATGC(1/-1) 1477 GAATGC Bsp1407I 293 TGTACA T^GTACA 1222 TGTACA BsrGI 293 TGTACA T^GTACA 1222 TGTACA BstYI 594 AGATCC [GA]^GATC[CT] 1104 GGATCC Cac8I 53 GCTTGC GC[ACGT]^[ACGT]GC 661 GCATGC CfoI 4 GCGC GCG^C 28 GCGC DraII 418 AGGACCC [GA]G^G[ACGT]CC[CT] 786 AGGTCCT EarI 95 CTCTTC CTCTTC(1/4) 835 GAAGAG EcoO109I 418 AGGACCC [GA]G^G[ACGT]CC[CT] 786 AGGTCCT EcoRI 1162 GAATTC G^AATTC 1211 GAATTC EcoT14I 1129 CCAAGG C^C[AT][AT]GG 1341 CCAAGG HaeII 3 GGCGCC [GA]GCGC^[CT] 27 GGCGCT HhaI 4 GCGC GCG^C 28 GCGC HinP1I 4 GCGC G^CGC 28 GCGC HinfI 1018 GATTC G^A[ACGT]TC 1353 GACTC Ksp632I 95 CTCTTC CTCTTC(1/4) 835 GAAGAG MflI 594 AGATCC [GA]^GATC[CT] 1104 GGATCC NdeI 986 CATATG CA^TATG 1391 CATATG PpuMI 418 AGGACCC [GA]G^G[AT]CC[CT] 786 AGGTCCT PshBI 145 ATTAAT AT^TAAT 772 ATTAAT ScaI 745 AGTACT AGT^ACT 1217 AGTACT SexAI 887 ACCAGGT A^CC[AT]GGT 1092 ACCTGGT SspBI 293 TGTACA T^GTACA 1222 TGTACA StyI 1129 CCAAGG C^C[AT][AT]GG 1341 CCAAGG XhoII 594 AGATCC [GA]^GATC[CT] 1104 GGATCC XmnI 91 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 904 GAAAAACTTC ######################################### # 3 OR MORE CUT ENZYMES ( 50 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI AvaII BbsI BbvI BfaI BmyI BpuAI BsaJI BsgI BsiYI BslI Bsp1286I BsrI BssKI BstNI Cfr13I DdeI DpnII EcoRII Fnu4HI HapII HpaII HphI ItaI MaeI MboI MboII MnlI MseI MslI MspI MvaI MwoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI Tru9I TseI Tsp509I TspRI