################################## # NO CUT ENZYMES ( 160 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BspEI BspLU11I BspMI BsrDI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstYI BstZ17I Bsu36I CelII Cfr10I CpoI DraII DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV FbaI FseI HaeII HapII HgaI Hin1I HindIII HpaI HpaII KasI KpnI KspI MamI MfeI MflI MluI MluNI MroI MscI MspA1I MspI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NlaIV NotI NruI NspI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AccI 592 GTAGAC GT^[AC][GT]AC AcsI 103 GAATTT [GA]^AATT[CT] ApoI 103 GAATTT [GA]^AATT[CT] AspI 874 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 92 CTCGGG C^[CT]CG[GA]G AviII 335 TGCGCA TGC^GCA BanII 136 GAGCTC G[GA]GC[CT]^C BbvI 958 GCAGC GCAGC(8/12) BglI 358 GCCTGCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaJI 422 CCATGG C^C[ACGT][ACGT]GG BsiYI 571 CCATTTTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 571 CCATTTTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmI 553 GCATTC GAATGC(1/-1) BsoBI 92 CTCGGG C^[CT]CG[GA]G Bsp1407I 929 TGTACA T^GTACA BspDI 69 ATCGAT AT^CGAT BspHI 476 TCATGA T^CATGA BsrBI 780 CCGCTC CCGCTC(-3/-3) BsrGI 929 TGTACA T^GTACA BstXI 542 CCAGCCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CfoI 336 GCGC GCG^C ClaI 69 ATCGAT AT^CGAT DpnII 19 GATC ^GATC DraI 915 TTTAAA TTT^AAA DsaI 422 CCATGG C^C[GA][CT]GG EcoT14I 422 CCATGG C^C[AT][AT]GG EcoT22I 831 ATGCAT ATGCA^T Fnu4HI 958 GCAGC GC^[ACGT]GC FspI 335 TGCGCA TGC^GCA HhaI 336 GCGC GCG^C HinP1I 336 GCGC G^CGC HincII 871 GTTGAC GT[CT]^[GA]AC HindII 871 GTTGAC GT[CT]^[GA]AC HphI 96 GGTGA GGTGA(8/7) ItaI 958 GCAGC GC^[ACGT]GC MaeII 281 ACGT A^CGT MboI 19 GATC ^GATC NcoI 422 CCATGG C^CATGG NdeII 19 GATC ^GATC NsiI 831 ATGCAT ATGCA^T PflFI 874 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 571 CCATTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 978 GAGTC GAGTC(4/5) RcaI 476 TCATGA T^CATGA SacI 136 GAGCTC GAGCT^C Sau3AI 19 GATC ^GATC SfaNI 355 GATGC GCATC(5/9) SfcI 1007 CTGTAG C^T[GA][CT]AG SspBI 929 TGTACA T^GTACA StyI 422 CCATGG C^C[AT][AT]GG TaqI 70 TCGA T^CGA TseI 958 GCAGC G^C[AT]GC Tth111I 874 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 70 TCGA T^CGA Van91I 571 CCATTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AciI 231 CCGC CCGC(-3/-1) 780 CCGC AfaI 249 GTAC GT^AC 930 GTAC AvaII 580 GGTCC G^G[AT]CC 826 GGACC BseRI 706 CTCCTC GAGGAG(10/8) 756 CTCCTC BsmAI 79 GAGAC GTCTC(1/5) 718 GTCTC BsmFI 117 GGGAC GGGAC(10/14) 57 GTCCC BsrI 394 ACTGG ACTGG(1/-1) 546 CCAGT BssKI 363 CCTGG ^CC[ACGT]GG 515 CCTGG BstF5I 207 CATCC GGATG(2/0) 531 CATCC BstNI 363 CCTGG CC^[AT]GG 515 CCTGG EarI 388 CTCTTC CTCTTC(1/4) 975 GAAGAG EcoRII 363 CCTGG ^CC[AT]GG 515 CCTGG FauI 230 CCCGC CCCGC(4/6) 779 CCCGC FokI 207 CATCC GGATG(9/13) 531 CATCC Ksp632I 388 CTCTTC CTCTTC(1/4) 975 GAAGAG MvaI 363 CCTGG CC^[AT]GG 515 CCTGG RsaI 249 GTAC GT^AC 930 GTAC ScrFI 363 CCTGG CC^[ACGT]GG 515 CCTGG TfiI 226 GATTC G^A[AT]TC 750 GAATC ######################################### # 3 OR MORE CUT ENZYMES ( 24 enzymes) # ######################################### AluI AspHI BfaI BmyI BsiHKAI Bsp1286I Cac8I Cfr13I DdeI HaeIII HinfI MaeI MaeIII MboII MnlI MseI MslI MwoI NlaIII Sau96I Tru9I Tsp45I Tsp509I TspRI