################################## # NO CUT ENZYMES ( 125 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AgeI Alw44I AlwNI ApaLI AscI Asp718I AspI AvaI AviII AvrII BalI BbeI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BsaAI BsaBI BsaHI BsaI BseAI BsiEI BsiWI BsoBI BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstXI BstZ17I Cfr10I ClaI CpoI DraI DraIII DrdI EaeI EagI EclXI Eco52I EcoNI EcoRI EcoT22I FbaI FseI FspI HgaI Hin1I HindIII HpaI KasI KpnI KspI MaeI MamI MluI MluNI MroI MscI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SwaI Tth111I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AcsI 1936 AAATTC [GA]^AATT[CT] AflIII 990 ACGTGT A^C[GA][CT]GT AhdI 1625 GACCCACCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 1386 AGCGCT AGC^GCT ApoI 1936 AAATTC [GA]^AATT[CT] AseI 1393 ATTAAT AT^TAAT AsnI 1393 ATTAAT AT^TAAT Asp700I 627 GAAAGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 1625 GACCCACCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 1122 GAGCAC G[AT]GC[AT]^C BanI 1654 GGCACC G^G[CT][GA]CC BglI 1646 GCCTACCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 43 GCTGAGC GC^T[ACGT]AGC Bpu1102I 43 GCTGAGC GC^T[ACGT]AGC BsaWI 617 TCCGGT [AT]^CCGG[AT] BsiHKAI 1122 GAGCAC G[AT]GC[AT]^C BsmBI 1632 CGTCTC CGTCTC(1/5) Bsp1407I 1911 TGTACA T^GTACA BsrGI 1911 TGTACA T^GTACA CelII 43 GCTGAGC GC^T[ACGT]AGC CfoI 1387 GCGC GCG^C DsaI 1372 CCATGG C^C[GA][CT]GG Eam1105I 1625 GACCCACCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 1386 AGCGCT AGC^GCT EcoRV 805 GATATC GAT^ATC EcoT14I 1372 CCATGG C^C[AT][AT]GG FauI 691 GCGGG CCCGC(4/6) HaeII 1386 AGCGCT [GA]GCGC^[CT] HhaI 1387 GCGC GCG^C HinP1I 1387 GCGC G^CGC MaeII 990 ACGT A^CGT MfeI 281 CAATTG C^AATTG MslI 1467 CACCTCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MunI 281 CAATTG C^AATTG NcoI 1372 CCATGG C^CATGG NspI 1518 GCATGT [GA]CATG^[CT] PshBI 1393 ATTAAT AT^TAAT SapI 349 GAAGAGC GCTCTTC(1/4) ScaI 1400 AGTACT AGT^ACT SfcI 1763 CTGTAG C^T[GA][CT]AG SspBI 1911 TGTACA T^GTACA StuI 1823 AGGCCT AGG^CCT StyI 1372 CCATGG C^C[AT][AT]GG TaqI 1015 TCGA T^CGA Tsp45I 646 GTGAC ^GT[GC]AC TthHB8I 1015 TCGA T^CGA XmnI 627 GAAAGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AfaI 1401 GTAC GT^AC 1912 GTAC AocI 85 CCTCAGG CC^T[ACGT]AGG 883 CCTAAGG ApaI 1242 GGGCCC GGGCC^C 1966 GGGCCC BamHI 1321 GGATCC G^GATCC 1917 GGATCC BpmI 316 CTCCAG CTGGAG(16/14) 1856 CTGGAG BsmAI 224 GAGAC GTCTC(1/5) 1633 GTCTC BsmI 400 GAATGC GAATGC(1/-1) 1079 GCATTC BsrDI 1587 CATTGC GCAATG(2/0) 1028 GCAATG BstEII 645 GGTGACC G^GT[ACGT]ACC 1757 GGTTACC BstPI 645 GGTGACC G^GT[ACGT]ACC 1757 GGTTACC Bsu36I 85 CCTCAGG CC^T[ACGT]AGG 883 CCTAAGG Eco81I 85 CCTCAGG CC^T[ACGT]AGG 883 CCTAAGG EcoO65I 645 GGTGACC G^GT[ACGT]ACC 1757 GGTTACC HincII 1061 GTCAAC GT[CT]^[GA]AC 2014 GTTGAC HindII 1061 GTCAAC GT[CT]^[GA]AC 2014 GTTGAC PvuII 680 CAGCTG CAG^CTG 1946 CAGCTG RsaI 1401 GTAC GT^AC 1912 GTAC SmlI 597 CTCAAG C^T[CT][GA]AG 1744 CTCAAG SspI 482 AATATT AAT^ATT 778 AATATT ######################################### # 3 OR MORE CUT ENZYMES ( 67 enzymes) # ######################################### AciI AluI AlwI AvaII BanII BbsI BbvI BcnI BmyI BpuAI BsaJI BseRI BsgI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PflMI PleI PpuMI Sau3AI Sau96I ScrFI SfaNI TfiI Tru9I TseI Tsp509I TspRI Van91I XhoII