(Circular) (Six-base) MAPSORT of: pFN21AE2053.seq Check: 9387 from: 1 to: 6769 pFN21AE2053 6769 bp With 182 enzymes: SgfI * August 16, 2010 14:52 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4579 278 Size: 53 83 186 936 3043 2468 Fragments arranged by size: 3043 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4112 1937 Size: 2175 4594 Acc65I G'GTAC_C Cuts at: 4090 4090 Size: 6769 AclI AA'CG_TT Cuts at: 1563 4896 5269 1563 Size: 3333 373 3063 Fragments arranged by size: 3333 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2160 2256 4832 6216 6484 1632 Size: 528 96 2576 1384 268 1917 Fragments arranged by size: 2576 1917 1384 528 268 96 AfeI AGC'GCT Cuts at: 3393 3393 Size: 6769 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6572 AflIII A'CryG_T Cuts at: 1203 2995 5668 6645 1203 Size: 1792 2673 977 1327 Fragments arranged by size: 2673 1792 1327 977 AgeI A'CCGG_T Cuts at: 5600 5600 Size: 6769 AhdI GACnn_n'nnGTC Cuts at: 3635 5498 3635 Size: 1863 4906 AleI CACnn'nnGTG Cuts at: 1166 3476 1166 Size: 2310 4459 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6769 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2621 2653 2621 Size: 32 6737 AlwNI CAG_nnn'CTG Cuts at: 4512 6084 4512 Size: 1572 5197 ApaI G_GGCC'C Cuts at: 3251 3975 3251 Size: 724 6045 ApaLI G'TGCA_C Cuts at: 4826 5982 4826 Size: 1156 5613 ApoI r'AATT_y Cuts at: 1493 3941 4078 4304 4456 6565 1493 Size: 2448 137 226 152 2109 1697 Fragments arranged by size: 2448 2109 1697 226 152 137 AseI AT'TA_AT Cuts at: 160 1032 3399 5323 160 Size: 872 2367 1924 1606 Fragments arranged by size: 2367 1924 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6769 AvaI C'yCGr_G Cuts at: 1870 1943 4094 1870 Size: 73 2151 4545 Fragments arranged by size: 4545 2151 73 BamHI G'GATC_C Cuts at: 3326 3922 4099 4466 3326 Size: 596 177 367 5629 Fragments arranged by size: 5629 596 367 177 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3659 4090 5545 618 Size: 325 217 627 1872 431 1455 1842 Fragments arranged by size: 1872 1842 1455 627 431 325 217 BanII G_rGCy'C Cuts at: 1391 3251 3975 3996 4088 1391 Size: 1860 724 21 92 4072 Fragments arranged by size: 4072 1860 724 92 21 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2901 3123 3355 4040 928 Size: 971 1002 222 232 685 3657 Fragments arranged by size: 3657 1002 971 685 232 222 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4960 4994 1903 Size: 34 3023 34 3678 Fragments arranged by size: 3678 3023 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4661 5871 4661 Size: 1210 5559 BclI T'GATC_A Cuts at: 1548 1548 Size: 6769 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3657 5380 136 Size: 107 122 71 631 605 1985 1723 1525 Fragments arranged by size: 1985 1723 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6764 6764 Size: 6769 BlpI GC'TnA_GC Cuts at: 2049 4186 2049 Size: 2137 4632 Bme1580I G_kGCm'C Cuts at: 1790 3251 3975 4830 5986 1790 Size: 1461 724 855 1156 2573 Fragments arranged by size: 2573 1461 1156 855 724 BmgBI CAC'GTC Cuts at: 1318 2996 1318 Size: 1678 5091 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2097 3418 3611 Size: 416 53 83 399 24 680 1321 193 Cuts at: 3611 3970 4500 5458 442 Size: 359 530 958 1753 Fragments arranged by size: 1753 1321 958 680 530 416 399 359 193 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6769 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2820 2820 Size: 6769 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2306 3882 5429 1239 Size: 1067 1576 1547 2579 Fragments arranged by size: 2579 1576 1547 1067 Bpu10I CC'TnA_GC Cuts at: 3519 3519 Size: 6769 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2587 3734 4894 5759 6057 2587 Size: 1147 1160 865 298 3299 Fragments arranged by size: 3299 1160 1147 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5432 882 Size: 617 3933 2219 Fragments arranged by size: 3933 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4560 493 Size: 711 3356 2702 Fragments arranged by size: 3356 2702 711 BsaBI GATnn'nnATC Cuts at: 6763 6763 Size: 6769 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4576 4958 5593 275 Size: 53 83 186 936 3043 382 635 1451 Fragments arranged by size: 3043 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2622 5202 5600 5874 6021 6432 2622 Size: 2580 398 274 147 411 2959 Fragments arranged by size: 2959 2580 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2060 2090 2236 2266 2456 Size: 30 165 30 657 30 146 30 190 Cuts at: 2456 2486 2621 2651 3625 3655 1178 Size: 30 135 30 974 30 4292 Fragments arranged by size: 4292 974 657 190 165 146 135 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2275 3179 3207 3879 1186 Size: 1089 904 28 672 4076 Fragments arranged by size: 4076 1089 904 672 28 BseYI C'CCAG_C Cuts at: 3228 3244 3711 5972 6468 3228 Size: 16 467 2261 496 3529 Fragments arranged by size: 3529 2261 496 467 16 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2234 2532 2975 3500 1163 Size: 573 498 298 443 525 4432 Fragments arranged by size: 4432 573 525 498 443 298 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4219 4980 6008 1540 Size: 275 186 2218 761 1028 2301 Fragments arranged by size: 2301 2218 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3131 4830 4915 5986 3131 Size: 1699 85 1071 3914 Fragments arranged by size: 3914 1699 1071 85 BsmI GAATG_Cn' Cuts at: 2411 3084 4280 4373 2411 Size: 673 1196 93 4807 Fragments arranged by size: 4807 1196 673 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3643 3643 Size: 6769 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3131 3251 3975 3996 4088 4830 4915 Size: 399 1341 120 724 21 92 742 85 Cuts at: 4915 5986 1391 Size: 1071 2174 Fragments arranged by size: 2174 1341 1071 742 724 399 120 92 85 21 BspEI T'CCGG_A Cuts at: 6432 6432 Size: 6769 BspHI T'CATG_A Cuts at: 4658 6388 4658 Size: 1730 5039 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4124 844 Size: 3280 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4656 1394 Size: 429 2833 3507 Fragments arranged by size: 3507 2833 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3040 3591 5264 5438 65 Size: 1183 1792 551 1673 174 1396 Fragments arranged by size: 1792 1673 1396 1183 551 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5413 5600 1700 Size: 110 3603 187 2869 Fragments arranged by size: 3603 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3916 96 Size: 3820 2949 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6769 BssSI C'ACGA_G Cuts at: 4829 5841 4829 Size: 1012 5757 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6769 BstBI TT'CG_AA Cuts at: 5573 5573 Size: 6769 BstEII G'GTnAC_C Cuts at: 1184 2650 3762 1184 Size: 1466 1112 4191 Fragments arranged by size: 4191 1466 1112 BstYI r'GATC_y Cuts at: 1972 2070 2619 2872 3326 3922 4099 4466 4851 Size: 98 549 253 454 596 177 367 385 Cuts at: 4851 4868 6320 6406 6418 6764 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 596 549 454 385 367 346 253 177 98 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2091 2889 2091 Size: 798 5971 BtgI C'CryG_G Cuts at: 513 1065 3377 513 Size: 552 2312 3905 Fragments arranged by size: 3905 2312 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6030 Fragments arranged by size: 6030 683 56 BtsI GCAGTG_nn' Cuts at: 2536 2544 4285 5079 5099 2536 Size: 8 1741 794 20 4206 Fragments arranged by size: 4206 1741 794 20 8 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6769 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6734 DraI TTT'AAA Cuts at: 4073 4073 Size: 6769 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5776 809 Size: 507 4460 1802 Fragments arranged by size: 4460 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4216 5105 6734 Size: 54 1425 50 275 18 2386 889 1629 Cuts at: 6734 8 Size: 43 Fragments arranged by size: 2386 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4216 1537 Size: 275 2404 4090 Fragments arranged by size: 4090 2404 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2289 2349 3670 3722 4699 1353 Size: 936 60 1321 52 977 3423 Fragments arranged by size: 3423 1321 977 936 60 52 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5356 5730 5876 387 Size: 1064 138 3767 374 146 1280 Fragments arranged by size: 3767 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2160 2256 2306 3882 4832 5429 6216 Size: 393 528 96 50 1576 950 597 787 Cuts at: 6216 6484 1239 Size: 268 1524 Fragments arranged by size: 1576 1524 950 787 597 528 393 268 96 50 EcoO109I rG'GnC_Cy Cuts at: 3629 3691 3906 3971 3972 3984 3629 Size: 62 215 65 1 12 6414 Fragments arranged by size: 6414 215 65 62 12 1 EcoRI G'AATT_C Cuts at: 4078 4456 4078 Size: 378 6391 EcoRV GAT'ATC Cuts at: 2812 2812 Size: 6769 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2359 2359 Size: 6769 FspI TGC'GCA Cuts at: 5275 5275 Size: 6769 HaeII r_GCGC'y Cuts at: 1678 3395 5916 1678 Size: 1717 2521 2531 Fragments arranged by size: 2531 2521 1717 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2060 2092 2554 2586 2950 Size: 32 135 32 488 32 462 32 364 Cuts at: 2950 2982 3409 3441 3499 3531 3621 3653 5410 Size: 32 427 32 58 32 90 32 1757 Cuts at: 5410 5442 5484 5516 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1757 488 462 427 364 135 90 58 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3068 4021 4113 4359 669 Size: 988 281 1130 953 92 246 3079 Fragments arranged by size: 3079 1130 988 953 281 246 92 HindIII A'AGCT_T Cuts at: 748 748 Size: 6769 HpaI GTT'AAC Cuts at: 4359 4359 Size: 6769 KpnI G_GTAC'C Cuts at: 4094 4094 Size: 6769 MfeI C'AATT_G Cuts at: 2286 4368 2286 Size: 2082 4687 MluI A'CGCG_T Cuts at: 6645 6645 Size: 6769 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2607 3693 5883 6067 1305 Size: 1302 1086 2190 184 2007 Fragments arranged by size: 2190 2007 1302 1086 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4947 Fragments arranged by size: 4947 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3476 4727 5086 5245 518 Size: 648 2310 1251 359 159 2042 Fragments arranged by size: 2310 2042 1251 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2687 2696 3953 4185 4862 6010 6255 1606 Size: 1081 9 1257 232 677 1148 245 2120 Fragments arranged by size: 2120 1257 1148 1081 677 245 232 9 NcoI C'CATG_G Cuts at: 513 1065 3377 513 Size: 552 2312 3905 Fragments arranged by size: 3905 2312 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6769 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6769 NotI GC'GGCC_GC Cuts at: 4216 4216 Size: 6769 NspI r_CATG'y Cuts at: 1132 1229 3527 4127 5672 1132 Size: 97 2298 600 1545 2229 Fragments arranged by size: 2298 2229 1545 600 97 PasI CC'CwG_GG Cuts at: 1348 2771 1348 Size: 1423 5346 PciI A'CATG_T Cuts at: 5668 5668 Size: 6769 PflMI CCAn_nnn'nTGG Cuts at: 1108 2647 3278 3383 1108 Size: 1539 631 105 4494 Fragments arranged by size: 4494 1539 631 105 PfoI T'CCnGG_A Cuts at: 3538 3538 Size: 6769 PmeI GTTT'AAAC Cuts at: 4073 4073 Size: 6769 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2621 2653 4838 4870 6351 6383 2621 Size: 32 2185 32 1481 32 3007 Fragments arranged by size: 3007 2185 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3629 3691 3984 3629 Size: 62 293 6414 Fragments arranged by size: 6414 293 62 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6769 PsiI TTA'TAA Cuts at: 4339 4339 Size: 6769 PspOMI G'GGCC_C Cuts at: 3247 3971 3247 Size: 724 6045 PstI C_TGCA'G Cuts at: 830 4121 830 Size: 3291 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6769 PvuII CAG'CTG Cuts at: 2687 3953 2687 Size: 1266 5503 SalI G'TCGA_C Cuts at: 1936 4111 1936 Size: 2175 4594 SanDI GG'GwC_CC Cuts at: 3629 3629 Size: 6769 SapI GCTCTTCn'nnn_ Cuts at: 2349 2349 Size: 6769 SbfI CC_TGCA'GG Cuts at: 4121 4121 Size: 6769 ScaI AGT'ACT Cuts at: 3407 5017 6729 3407 Size: 1610 1712 3447 Fragments arranged by size: 3447 1712 1610 SfcI C'TryA_G Cuts at: 826 1045 3768 4117 5252 5933 6124 826 Size: 219 2723 349 1135 681 191 1471 Fragments arranged by size: 2723 1471 1135 681 349 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6769 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6769 SmaI CCC'GGG Cuts at: 1872 4096 1872 Size: 2224 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2602 3749 4873 5774 6036 820 Size: 197 926 659 1147 1124 901 262 1553 Fragments arranged by size: 1553 1147 1124 926 901 659 262 197 SnaBI TAC'GTA Cuts at: 493 4560 493 Size: 4067 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6769 SphI G_CATG'C Cuts at: 1132 4127 1132 Size: 2995 3774 SspI AAT'ATT Cuts at: 5 52 2489 2785 4693 5 Size: 47 2437 296 1908 2081 Fragments arranged by size: 2437 2081 1908 296 47 StuI AGG'CCT Cuts at: 1517 3830 1517 Size: 2313 4456 StyI C'CwwG_G Cuts at: 513 1065 3377 4208 513 Size: 552 2312 831 3074 Fragments arranged by size: 3074 2312 831 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2183 3220 3400 4796 4813 4966 5151 Size: 1255 603 1037 180 1396 17 153 185 Cuts at: 5151 5596 325 Size: 445 1498 Fragments arranged by size: 1498 1396 1255 1037 603 445 185 180 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3405 3916 5015 Size: 274 80 33 51 1443 1428 511 1099 Cuts at: 5015 6727 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1428 1099 511 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2236 2268 3625 3657 6729 6761 2236 Size: 32 1357 32 3072 32 2244 Fragments arranged by size: 3072 2244 1357 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6769 XbaI T'CTAG_A Cuts at: 4105 4105 Size: 6769 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6769 XmaI C'CCGG_G Cuts at: 1870 4094 1870 Size: 2224 4545 XmnI GAAnn'nnTTC Cuts at: 2636 4898 2636 Size: 2262 4507 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4577 276 Size: 53 83 186 936 3043 2468 Fragments arranged by size: 3043 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SexAI SfiI SfoI SrfI SwaI XcmI