Restriction map for pFN21AE9396
 (Circular) (Six-base) MAPSORT of: pFN21AE9396.seq  Check: 7999  from: 1  to: 16612



pFN21AE9396  16612 bp

 With 182 enzymes: SgfI * 

                        May 26, 2016 14:38  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:  13130   13547   13130 
   Size:        417   16195
 
AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    5455   14422     278 
   Size:         53      83     186     936    3919    8967    2468

  Fragments arranged by size: 

                8967    3919    2468     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    3338    7042    8166    8482   10846   11680   12465   13503 
   Size:       1401    3704    1124     316    2364     834     785    1038

Cuts at:  13503   13955    1937 
   Size:        452    4594

  Fragments arranged by size: 

                4594    3704    2364    1401    1124    1038     834     785
                 452     316
 
Acc65I G'GTAC_C

Cuts at:   4016   11101   12116   13933    4016 
   Size:       7085    1015    1817    6695

  Fragments arranged by size: 

                7085    6695    1817    1015
 
AclI AA'CG_TT

Cuts at:   1563   13911   14739   15112    1563 
   Size:      12348     828     373    3063

  Fragments arranged by size: 

               12348    3063     828     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2289    2487    3408    3546    4011    4173    6956    8844 
   Size:        657     198     921     138     465     162    2783    1888

Cuts at:   8844    8868    9054    9216   10008   10059   10299   10527   10980 
   Size:         24     186     162     792      51     240     228     453

Cuts at:  10980   11081   11237   11346   11532   12344   12414   12573   12720 
   Size:        101     156     109     186     812      70     159     147

Cuts at:  12720   12938   13323   13433   14675   16059   16327    1632 
   Size:        218     385     110    1242    1384     268    1917

  Fragments arranged by size: 

                2783    1917    1888    1384    1242     921     812     792
                 657     465     453     385     268     240     228     218
                 198     186     186     162     162     159     156     147
                 138     110     109     101      70      51      24
 
AfeI AGC'GCT

Cuts at:   8195    8195 
   Size:      16612
 
AflII C'TTAA_G

Cuts at:    820    1017   10015     820 
   Size:        197    8998    7417

  Fragments arranged by size: 

                8998    7417     197
 
AflIII A'CryG_T

Cuts at:   1203    2104    5212    7294    7702    9882   10300   12085   13332 
   Size:        901    3108    2082     408    2180     418    1785    1247

Cuts at:  13332   15511   16488    1203 
   Size:       2179     977    1327

  Fragments arranged by size: 

                3108    2180    2179    2082    1785    1327    1247     977
                 901     418     408
 
AgeI A'CCGG_T

Cuts at:  15443   15443 
   Size:      16612
 
AhdI GACnn_n'nnGTC

Cuts at:   8893   15341    8893 
   Size:       6448   10164
 
AleI CACnn'nnGTG

Cuts at:   1166    6456    6866    7169   13728    1166 
   Size:       5290     410     303    6559    4050

  Fragments arranged by size: 

                6559    5290    4050     410     303
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    2396    4482    6138    8895   11477   12302   13460   13719 
   Size:       1212    2086    1656    2757    2582     825    1158     259

Cuts at:  13719   13883    1184 
   Size:        164    3913

  Fragments arranged by size: 

                3913    2757    2582    2086    1656    1212    1158     825
                 259     164
 
AlwNI CAG_nnn'CTG

Cuts at:   6490    9689   10705   10721   11228   11993   12172   12767   14355 
   Size:       3199    1016      16     507     765     179     595    1588

Cuts at:  14355   15927    6490 
   Size:       1572    7175

  Fragments arranged by size: 

                7175    3199    1588    1572    1016     765     595     507
                 179      16
 
ApaI G_GGCC'C

Cuts at:   2136    2333   12438    2136 
   Size:        197   10105    6310

  Fragments arranged by size: 

               10105    6310     197
 
ApaLI G'TGCA_C

Cuts at:   9870   14669   15825    9870 
   Size:       4799    1156   10657

  Fragments arranged by size: 

               10657    4799    1156
 
ApoI r'AATT_y

Cuts at:   1493    2560    4698    6180    6443    6961    7805    8100    8755 
   Size:       1067    2138    1482     263     518     844     295     655

Cuts at:   8755    8797    8985   10459   13020   13235   13921   14147   14299 
   Size:         42     188    1474    2561     215     686     226     152

Cuts at:  14299   16408    1493 
   Size:       2109    1697

  Fragments arranged by size: 

                2561    2138    2109    1697    1482    1474    1067     844
                 686     655     518     295     263     226     215     188
                 152      42
 
AseI AT'TA_AT

Cuts at:    160    1032    2964    7218   15166     160 
   Size:        872    1932    4254    7948    1606

  Fragments arranged by size: 

                7948    4254    1932    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:      16612
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2042    2129    2156    2332    3977    4568    7025 
   Size:         73      99      87      27     176    1645     591    2457

Cuts at:   7025    7881   13749   13937    1870 
   Size:        856    5868     188    4545

  Fragments arranged by size: 

                5868    4545    2457    1645     856     591     188     176
                  99      87      73      27
 
AvrII C'CTAG_G

Cuts at:   2083    2083 
   Size:      16612
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:   9183    9216   10176   10209   10426   10459   11611   11644    9183 
   Size:         33     960      33     217      33    1152      33   14151

  Fragments arranged by size: 

               14151    1152     960     217      33      33      33      33
            
 
BamHI G'GATC_C

Cuts at:   5755    6614   11581   13942   14309    5755 
   Size:        859    4967    2361     367    8058

  Fragments arranged by size: 

                8058    4967    2361     859     367
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2073    2090    2161    2372    4016 
   Size:        325     217     627     286      17      71     211    1644

Cuts at:   4016    5422    5491    6885    9544   11101   11899   12116   13211 
   Size:       1406      69    1394    2659    1557     798     217    1095

Cuts at:  13211   13883   13933   15388     618 
   Size:        672      50    1455    1842

  Fragments arranged by size: 

                2659    1842    1644    1557    1455    1406    1394    1095
                 798     672     627     325     286     217     217     211
                  71      69      50      17
 
BanII G_rGCy'C

Cuts at:   1391    2136    2333    3154    4088    4102    4305    5985    6366 
   Size:        745     197     821     934      14     203    1680     381

Cuts at:   6366    8906    8951   10488   10604   10940   12438   13931    1391 
   Size:       2540      45    1537     116     336    1498    1493    4072

  Fragments arranged by size: 

                4072    2540    1680    1537    1498    1493     934     821
                 745     381     336     203     197     116      45      14
            
 
BbeI G_GCGC'C

Cuts at:   2077    2094    2165   13887    2077 
   Size:         17      71   11722    4802

  Fragments arranged by size: 

               11722    4802      71      17
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2268    2274    2680    3209    3414    4317    4803 
   Size:        971     369       6     406     529     205     903     486

Cuts at:   4803    5313    7578    7614    8930    9939   10038     928 
   Size:        510    2265      36    1316    1009      99    7502

  Fragments arranged by size: 

                7502    2265    1316    1009     971     903     529     510
                 486     406     369     205      99      36       6
 
BbvCI CC'TCA_GC

Cuts at:   2248    2296    3904    5925    6143   12511    2248 
   Size:         48    1608    2021     218    6368    6349

  Fragments arranged by size: 

                6368    6349    2021    1608     218      48
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    2000    2034    2241    2275   14803   14837    1903 
   Size:         34      63      34     207      34   12528      34    3678

  Fragments arranged by size: 

               12528    3678     207      63      34      34      34      34
            
 
BciVI GTATCCnnnnn_n'

Cuts at:   4207    4731    4852    6071    9861   11962   12046   12454   12772 
   Size:        524     121    1219    3790    2101      84     408     318

Cuts at:  12772   14504   15714    4207 
   Size:       1732    1210    5105

  Fragments arranged by size: 

                5105    3790    2101    1732    1219    1210     524     408
                 318     121      84
 
BclI T'GATC_A

Cuts at:   1548    7108    7779    7944    8734   10978    1548 
   Size:       5560     671     165     790    2244    7182

  Fragments arranged by size: 

                7182    5560    2244     790     671     165
 
BfrBI ATG'CAT

Cuts at:  13696   13696 
   Size:      16612
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2088    2195   11942 
   Size:        107     122      71     631     605     416     107    9747

Cuts at:  11942   15223     136 
   Size:       3281    1525

  Fragments arranged by size: 

                9747    3281    1525     631     605     416     122     107
                 107      71
 
BglII A'GATC_T

Cuts at:   4250    7123   11867   13044   13803   16607    4250 
   Size:       2873    4744    1177     759    2804    4255

  Fragments arranged by size: 

                4744    4255    2873    2804    1177     759
 
BlpI GC'TnA_GC

Cuts at:   3120    3180    5100   10401   10406   10934   12768   13291   14029 
   Size:         60    1920    5301       5     528    1834     523     738

Cuts at:  14029    3120 
   Size:       5703

  Fragments arranged by size: 

                5703    5301    1920    1834     738     528     523      60
                   5
 
Bme1580I G_kGCm'C

Cuts at:   1790    2136    2333    3364    3661    9868    9874   11148   12438 
   Size:        346     197    1031     297    6207       6    1274    1290

Cuts at:  12438   14673   15829    1790 
   Size:       2235    1156    2573

  Fragments arranged by size: 

                6207    2573    2235    1290    1274    1156    1031     346
                 297     197       6
 
BmgBI CAC'GTC

Cuts at:   1318   12088    1318 
   Size:      10770    5842
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3444    3547    8442 
   Size:        416      53      83     399      24    2027     103    4895

Cuts at:   8442    8658    9969   10836   11334   12239   12402   14343   15301 
   Size:        216    1311     867     498     905     163    1941     958

Cuts at:  15301     442 
   Size:       1753

  Fragments arranged by size: 

                4895    2027    1941    1753    1311     958     905     867
                 498     416     399     216     163     103      83      53
                  24
 
BmtI G_CTAG'C

Cuts at:   1056   11454   12162   12789    1056 
   Size:      10398     708     627    4879

  Fragments arranged by size: 

               10398    4879     708     627
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4144    9115    9850   11405   12817   13838    4144 
   Size:       4971     735    1555    1412    1021    6918

  Fragments arranged by size: 

                6918    4971    1555    1412    1021     735
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2643    2712    3715    4973    5421    5853    6425    6512 
   Size:       1404      69    1003    1258     448     432     572      87

Cuts at:   6512    6609    6996    7521    8191    8217    8563    9165   12509 
   Size:         97     387     525     670      26     346     602    3344

Cuts at:  12509   12738   15272    1239 
   Size:        229    2534    2579

  Fragments arranged by size: 

                3344    2579    2534    1404    1258    1003     670     602
                 572     525     448     432     387     346     229      97
                  87      69      26
 
Bpu10I CC'TnA_GC

Cuts at:   2248    2296    3778    3904    5037    5925    6143    6262    8095 
   Size:         48    1482     126    1133     888     218     119    1833

Cuts at:   8095   12511    2248 
   Size:       4416    6349

  Fragments arranged by size: 

                6349    4416    1833    1482    1133     888     218     126
                 119      48
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2831    8041   11642   14737   15602   15900    2831 
   Size:       5210    3601    3095     865     298    3543

  Fragments arranged by size: 

                5210    3601    3543    3095     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2362    2502    2530    2606    2960    4441    4626 
   Size:        617     863     140      28      76     354    1481     185

Cuts at:   4626    5177    5902    6657    9135   11393   13515   15275     882 
   Size:        551     725     755    2478    2258    2122    1760    2219

  Fragments arranged by size: 

                2478    2258    2219    2122    1760    1481     863     755
                 725     617     551     354     185     140      76      28
            
 
BsaAI yAC'GTr

Cuts at:    493    1204   14403     493 
   Size:        711   13199    2702

  Fragments arranged by size: 

               13199    2702     711
 
BsaBI GATnn'nnATC

Cuts at:   8854   11872   16606    8854 
   Size:       3018    4734    8860

  Fragments arranged by size: 

                8860    4734    3018
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2074    2091    2162    5452 
   Size:         53      83     186     936     541      17      71    3290

Cuts at:   5452    8584   13884   14419   14801   15436     275 
   Size:       3132    5300     535     382     635    1451

  Fragments arranged by size: 

                5300    3290    3132    1451     936     635     541     535
                 382     186      83      71      53      17
 
BsaWI w'CCGG_w

Cuts at:   2994    6103    8625   11578   12847   15045   15443   15717   15864 
   Size:       3109    2522    2953    1269    2198     398     274     147

Cuts at:  15864   16275    2994 
   Size:        411    3331

  Fragments arranged by size: 

                3331    3109    2953    2522    2198    1269     411     398
                 274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2482    2512    4453    4483    7815 
   Size:         30     165      30    1079      30    1941      30    3332

Cuts at:   7815    7845   10112   10142   12736   12766    1178 
   Size:         30    2267      30    2594      30    5024

  Fragments arranged by size: 

                5024    3332    2594    2267    1941    1079     165      30
                  30      30      30      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2248    2832    2948    3247    3400    3421    3500    3521 
   Size:       1062     584     116     299     153      21      79      21

Cuts at:   3521    3667    4494    4586    5778    6401    7049    7512    8370 
   Size:        146     827      92    1192     623     648     463     858

Cuts at:   8370    8545    8896    9166   10133   10900   11565   13271   13412 
   Size:        175     351     270     967     767     665    1706     141

Cuts at:  13412   13415   13827    1186 
   Size:          3     412    3971

  Fragments arranged by size: 

                3971    1706    1192    1062     967     858     827     767
                 665     648     623     584     463     412     351     299
                 270     175     153     146     141     116      92      79
                  21      21       3
 
BseYI C'CCAG_C

Cuts at:   2219    3663    5901    6783    8610   10286   10829   10924   11784 
   Size:       1444    2238     882    1827    1676     543      95     860

Cuts at:  11784   12408   13162   15815   16311    2219 
   Size:        624     754    2653     496    2520

  Fragments arranged by size: 

                2653    2520    2238    1827    1676    1444     882     860
                 754     624     543     496      95
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    3414    7837    8946   12912   13071   13692    1163 
   Size:        573    1678    4423    1109    3966     159     621    4083

  Fragments arranged by size: 

                4423    4083    3966    1678    1109     621     573     159
            
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2142    2190    2241    2364    5459    8486 
   Size:        275     186     141      48      51     123    3095    3027

Cuts at:   8486   14062   14823   15851    1540 
   Size:       5576     761    1028    2301

  Fragments arranged by size: 

                5576    3095    3027    2301    1028     761     275     186
                 141     123      51      48
 
BsiHKAI G_wGCw'C

Cuts at:   4102    4305    4471    8177    8906    8951    9874   10488   12291 
   Size:        203     166    3706     729      45     923     614    1803

Cuts at:  12291   14673   14758   15829    4102 
   Size:       2382      85    1071    4885

  Fragments arranged by size: 

                4885    3706    2382    1803    1071     923     729     614
                 203     166      85      45
 
BsmI GAATG_Cn'

Cuts at:   3874    6127    9325   14123   14216    3874 
   Size:       2253    3198    4798      93    6270

  Fragments arranged by size: 

                6270    4798    3198    2253      93
 
BsmBI CGTCTCn'nnnn_

Cuts at:   3687   11178    3687 
   Size:       7491    9121
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2136    2333    3154    3364    3661    4088    4102 
   Size:        399     346     197     821     210     297     427      14

Cuts at:   4102    4305    4471    5985    6366    8177    8906    8951    9868 
   Size:        203     166    1514     381    1811     729      45     917

Cuts at:   9868    9874   10488   10604   10940   11148   12291   12438   13931 
   Size:          6     614     116     336     208    1143     147    1493

Cuts at:  13931   14673   14758   15829    1391 
   Size:        742      85    1071    2174

  Fragments arranged by size: 

                2174    1811    1514    1493    1143    1071     917     821
                 742     729     614     427     399     381     346     336
                 297     210     208     203     197     166     147     116
                  85      45      14       6
 
BspEI T'CCGG_A

Cuts at:   2994    8625   11578   12847   16275    2994 
   Size:       5631    2953    1269    3428    3331

  Fragments arranged by size: 

                5631    3428    3331    2953    1269
 
BspHI T'CATG_A

Cuts at:   6655    6673    8521   14501   16231    6655 
   Size:         18    1848    5980    1730    7036

  Fragments arranged by size: 

                7036    5980    1848    1730      18
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2344    2388    2819    5628    5884   12832   13130   13547 
   Size:       1500      44     431    2809     256    6948     298     417

Cuts at:  13547   13967     844 
   Size:        420    3489

  Fragments arranged by size: 

                6948    3489    2809    1500     431     420     417     298
                 256      44
 
BsrBI CCG'CTC

Cuts at:   1394    1823    2237    2412    3511    6883   13596   14499    1394 
   Size:        429     414     175    1099    3372    6713     903    3507

  Fragments arranged by size: 

                6713    3507    3372    1099     903     429     414     175
            
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    3114    6770    8286   11730   15107   15281      65 
   Size:       1183    1866    3656    1516    3444    3377     174    1396

  Fragments arranged by size: 

                3656    3444    3377    1866    1516    1396    1183     174
            
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    2311    2385    6993   11896   13580   15256   15443 
   Size:        110     501      74    4608    4903    1684    1676     187

Cuts at:  15443    1700 
   Size:       2869

  Fragments arranged by size: 

                4903    4608    2869    1684    1676     501     187     110
                  74
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:      16612
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:      16612
 
BssSI C'ACGA_G

Cuts at:   2529   13726   14672   15684    2529 
   Size:      11197     946    1012    3457

  Fragments arranged by size: 

               11197    3457    1012     946
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    3116    3914    5234   13647     794 
   Size:       2322     798    1320    8413    3759

  Fragments arranged by size: 

                8413    3759    2322    1320     798
 
BstBI TT'CG_AA

Cuts at:   8440    8566   15416    8440 
   Size:        126    6850    9636

  Fragments arranged by size: 

                9636    6850     126
 
BstEII G'GTnAC_C

Cuts at:   1184   12859    1184 
   Size:      11675    4937
 
BstXI CCAn_nnnn'nTGG

Cuts at:   7261   11347    7261 
   Size:       4086   12526
 
BstYI r'GATC_y

Cuts at:   1972    2578    3374    3620    4250    5755    6579    6614    7123 
   Size:        606     796     246     630    1505     824      35     509

Cuts at:   7123    8468    8849    8957    9260    9524    9917   10948   11581 
   Size:       1345     381     108     303     264     393    1031     633

Cuts at:  11581   11867   13044   13441   13803   13942   14309   14694   14711 
   Size:        286    1177     397     362     139     367     385      17

Cuts at:  14711   16163   16249   16261   16607    1972 
   Size:       1452      86      12     346    1977

  Fragments arranged by size: 

                1977    1505    1452    1345    1177    1031     824     796
                 633     630     606     509     397     393     385     381
                 367     362     346     303     286     264     246     139
                 108      86      35      17      12
 
BstZ17I GTA'TAC

Cuts at:   7043    8167    7043 
   Size:       1124   15488
 
Bsu36I CC'TnA_GG

Cuts at:   3504    5496    9822   12739   13313    3504 
   Size:       1992    4326    2917     574    6803

  Fragments arranged by size: 

                6803    4326    2917    1992     574
 
BtgI C'CryG_G

Cuts at:    513    1065    2004    2096    2190    5294    5325    7304    7830 
   Size:        552     939      92      94    3104      31    1979     526

Cuts at:   7830    9548   11376   13519     513 
   Size:       1718    1828    2143    3606

  Fragments arranged by size: 

                3606    3104    2143    1979    1828    1718     939     552
                 526      94      92      31
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56   15873

  Fragments arranged by size: 

               15873     683      56
 
BtsI GCAGTG_nn'

Cuts at:   3539    3716    3804    5480    5570    5704    7386    9868   10928 
   Size:        177      88    1676      90     134    1682    2482    1060

Cuts at:  10928   14128   14922   14942    3539 
   Size:       3200     794      20    5209

  Fragments arranged by size: 

                5209    3200    2482    1682    1676    1060     794     177
                 134      90      88      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:      16612
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564    3734    3769    5524    5559    6359    6394    7530 
   Size:         35    3170      35    1755      35     800      35    1136

Cuts at:   7530    7565    7676    7711     529 
   Size:         35     111      35    9430

  Fragments arranged by size: 

                9430    3170    1755    1136     800     111      35      35
                  35      35      35      35
 
DraI TTT'AAA

Cuts at:   3870    8913    9841   13916    3870 
   Size:       5043     928    4075    6566

  Fragments arranged by size: 

                6566    5043    4075     928
 
DraIII CAC_nnn'GTG

Cuts at:  11342   11918   11342 
   Size:        576   16036
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    3679   15619     809 
   Size:        507    2363   11940    1802

  Fragments arranged by size: 

               11940    2363    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2187    2238    2361 
   Size:         54    1425      50     275      18     357      51     123

Cuts at:   2361    5242    7684   10321   13522   14059   14948   16577       8 
   Size:       2881    2442    2637    3201     537     889    1629      43

  Fragments arranged by size: 

                3201    2881    2637    2442    1629    1425     889     537
                 357     275     123      54      51      50      43      18
            
 
EagI C'GGCC_G

Cuts at:   1537    1812    2187    2238    2361   14059    1537 
   Size:        275     375      51     123   11698    4090

  Fragments arranged by size: 

               11698    4090     375     275     123      51
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2308    2660    4095    4339    4514    5114    5218    7984 
   Size:        955     352    1435     244     175     600     104    2766

Cuts at:   7984    8602    8797    9073   10744   10871   11289   11326   12909 
   Size:        618     195     276    1671     127     418      37    1583

Cuts at:  12909   13389   14542    1353 
   Size:        480    1153    3423

  Fragments arranged by size: 

                3423    2766    1671    1583    1435    1153     955     618
                 600     480     418     352     276     244     195     175
                 127     104      37
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    2257    4816   15199   15573   15719     387 
   Size:       1064     138     668    2559   10383     374     146    1280

  Fragments arranged by size: 

               10383    2559    1280    1064     668     374     146     138
            
 
EcoICRI GAG'CTC

Cuts at:   4100    4303    8904    8949   10486    4100 
   Size:        203    4601      45    1537   10226

  Fragments arranged by size: 

               10226    4601    1537     203      45
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2289    2487    2643    2712    3408    3546    3715 
   Size:        393     657     198     156      69     696     138     169

Cuts at:   3715    4011    4173    4973    5421    5853    6425    6512    6609 
   Size:        296     162     800     448     432     572      87      97

Cuts at:   6609    6956    6996    7521    8191    8217    8563    8844    8868 
   Size:        347      40     525     670      26     346     281      24

Cuts at:   8868    9054    9165    9216   10008   10059   10299   10527   10980 
   Size:        186     111      51     792      51     240     228     453

Cuts at:  10980   11081   11237   11346   11532   12344   12414   12509   12573 
   Size:        101     156     109     186     812      70      95      64

Cuts at:  12573   12720   12738   12938   13323   13433   14675   15272   16059 
   Size:        147      18     200     385     110    1242     597     787

Cuts at:  16059   16327    1239 
   Size:        268    1524

  Fragments arranged by size: 

                1524    1242     812     800     792     787     696     670
                 657     597     572     525     453     448     432     393
                 385     347     346     296     281     268     240     228
                 200     198     186     186     169     162     156     156
                 147     138     111     110     109     101      97      95
                  87      70      69      64      51      51      40      26
                  24      18
 
EcoNI CCTnn'n_nnAGG

Cuts at:   3816    4955    5087    5937    8496   11547    3816 
   Size:       1139     132     850    2559    3051    8881

  Fragments arranged by size: 

                8881    3051    2559    1139     850     132
 
EcoO109I rG'GnC_Cy

Cuts at:   2080    2133    4564    5198    7067    7496    8428    9094   11372 
   Size:         53    2431     634    1869     429     932     666    2278

Cuts at:  11372   12434   12435   12599    2080 
   Size:       1062       1     164    6093

  Fragments arranged by size: 

                6093    2431    2278    1869    1062     932     666     634
                 429     164      53       1
 
EcoRI G'AATT_C

Cuts at:   6961    7805    8100    8755    8985   13921   14299    6961 
   Size:        844     295     655     230    4936     378    9274

  Fragments arranged by size: 

                9274    4936     844     655     378     295     230
 
EcoRV GAT'ATC

Cuts at:   8150    8251    8150 
   Size:        101   16511
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2517    2799    3145    3414    3944    4272    7291    2517 
   Size:        282     346     269     530     328    3019   11838

  Fragments arranged by size: 

               11838    3019     530     346     328     282     269
 
FseI GG_CCGG'CC

Cuts at:   2315    2315 
   Size:      16612
 
FspI TGC'GCA

Cuts at:  15118   15118 
   Size:      16612
 
HaeII r_GCGC'y

Cuts at:   1678    2029    2077    2094    2165    2351    6400    8197   13887 
   Size:        351      48      17      71     186    4049    1797    5690

Cuts at:  13887   15759    1678 
   Size:       1872    2531

  Fragments arranged by size: 

                5690    4049    2531    1872    1797     351     186      71
                  48      17
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2603    2616    2635    2648    2685 
   Size:         32     135      32    1031      13      19      13      37

Cuts at:   2685    2717    3216    3248    4453    4485    4562    4594    4891 
   Size:         32     499      32    1205      32      77      32     297

Cuts at:   4891    4923    5113    5145    5327    5330    5359    5362    5569 
   Size:         32     190      32     182       3      29       3     207

Cuts at:   5569    5601    5645    5677    6818    6850    7736    7768    8620 
   Size:         32      44      32    1141      32     886      32     852

Cuts at:   8620    8652    8699    8731    8879    8911    9590    9622    9647 
   Size:         32      47      32     148      32     679      32      25

Cuts at:   9647    9679   10772   10804   10872   10904   10963   10995   11000 
   Size:         32    1093      32      68      32      59      32       5

Cuts at:  11000   11032   11804   11836   12116   12148   12853   12885   13190 
   Size:         32     772      32     280      32     705      32     305

Cuts at:  13190   13222   15253   15285   15327   15359    1373 
   Size:         32    2031      32      42      32    2626

  Fragments arranged by size: 

                2626    2031    1205    1141    1093    1031     886     852
                 772     705     679     499     305     297     280     207
                 190     182     148     135      77      68      59      47
                  44      42      37      32      32      32      32      32
                  32      32      32      32      32      32      32      32
                  32      32      32      32      32      32      32      32
                  32      32      32      32      32      32      29      25
                  19      13      13       5       3       3
 
HincII GTy'rAC

Cuts at:    669    1657    1938    8483    9660   11248   13147   13956   14202 
   Size:        988     281    6545    1177    1588    1899     809     246

Cuts at:  14202     669 
   Size:       3079

  Fragments arranged by size: 

                6545    3079    1899    1588    1177     988     809     281
                 246
 
HindIII A'AGCT_T

Cuts at:    748    2870    5048   11416     748 
   Size:       2122    2178    6368    5944

  Fragments arranged by size: 

                6368    5944    2178    2122
 
HpaI GTT'AAC

Cuts at:  11248   13147   14202   11248 
   Size:       1899    1055   13658

  Fragments arranged by size: 

               13658    1899    1055
 
KasI G'GCGC_C

Cuts at:   2073    2090    2161   13883    2073 
   Size:         17      71   11722    4802

  Fragments arranged by size: 

               11722    4802      71      17
 
KpnI G_GTAC'C

Cuts at:   4020   11105   12120   13937    4020 
   Size:       7085    1015    1817    6695

  Fragments arranged by size: 

                7085    6695    1817    1015
 
MfeI C'AATT_G

Cuts at:   5349   11312   14211    5349 
   Size:       5963    2899    7750

  Fragments arranged by size: 

                7750    5963    2899
 
MluI A'CGCG_T

Cuts at:   2104   16488    2104 
   Size:      14384    2228
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    3585    3791    3982    7155    8142    8324    8483   10134 
   Size:       2280     206     191    3173     987     182     159    1651

Cuts at:  10134   10476   10976   12471   15726   15910    1305 
   Size:        342     500    1495    3255     184    2007

  Fragments arranged by size: 

                3255    3173    2280    2007    1651    1495     987     500
                 342     206     191     184     182     159
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    7686   10323      10 
   Size:         54    1425     343    5854    2637    6299

  Fragments arranged by size: 

                6299    5854    2637    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    3856    6456    6866    7169    7407    8844   10329 
   Size:        648    2690    2600     410     303     238    1437    1485

Cuts at:  10329   11001   12284   13701   13728   14570   14929   15088     518 
   Size:        672    1283    1417      27     842     359     159    2042

  Fragments arranged by size: 

                2690    2600    2042    1485    1437    1417    1283     842
                 672     648     410     359     303     238     159      27
            
 
MspA1I CmG'CkG

Cuts at:   1606    2016    2098    2192    2214    2430    3184    4022    4868 
   Size:        410      82      94      22     216     754     838     846

Cuts at:   4868    5231    6415    6487    7727    7795    8345    8614    9986 
   Size:        363    1184      72    1240      68     550     269    1372

Cuts at:   9986   10705   11192   11942   12269   12515   12574   12637   13204 
   Size:        719     487     750     327     246      59      63     567

Cuts at:  13204   13411   14028   14705   15853   16098    1606 
   Size:        207     617     677    1148     245    2120

  Fragments arranged by size: 

                2120    1372    1240    1184    1148     846     838     754
                 750     719     677     617     567     550     487     410
                 363     327     269     246     245     216     207      94
                  82      72      68      63      59      22
 
NaeI GCC'GGC

Cuts at:   2313   13582    2313 
   Size:      11269    5343
 
NarI GG'CG_CC

Cuts at:   2074    2091    2162   13884    2074 
   Size:         17      71   11722    4802

  Fragments arranged by size: 

               11722    4802      71      17
 
NcoI C'CATG_G

Cuts at:    513    1065    2004    5294    5325    7304   11376   13519     513 
   Size:        552     939    3290      31    1979    4072    2143    3606

  Fragments arranged by size: 

                4072    3606    3290    2143    1979     939     552      31
            
 
NdeI CA'TA_TG

Cuts at:    387    7770    9255     387 
   Size:       7383    1485    7744

  Fragments arranged by size: 

                7744    7383    1485
 
NgoMIV G'CCGG_C

Cuts at:   2311   13580    2311 
   Size:      11269    5343
 
NheI G'CTAG_C

Cuts at:   1052   11450   12158   12785    1052 
   Size:      10398     708     627    4879

  Fragments arranged by size: 

               10398    4879     708     627
 
NotI GC'GGCC_GC

Cuts at:   2187    2238   14059    2187 
   Size:         51   11821    4740

  Fragments arranged by size: 

               11821    4740      51
 
NsiI A_TGCA'T

Cuts at:  13698   13698 
   Size:      16612
 
NspI r_CATG'y

Cuts at:   1132    1229    5216    5275    6332    7706    8211    9886   10304 
   Size:         97    3987      59    1057    1374     505    1675     418

Cuts at:  10304   11260   12368   13336   13970   15515    1132 
   Size:        956    1108     968     634    1545    2229

  Fragments arranged by size: 

                3987    2229    1675    1545    1374    1108    1057     968
                 956     634     505     418      97      59
 
PasI CC'CwG_GG

Cuts at:   1348    5840    6294    1348 
   Size:       4492     454   11666

  Fragments arranged by size: 

               11666    4492     454
 
PciI A'CATG_T

Cuts at:   5212    7702    9882   10300   13332   15511    5212 
   Size:       2490    2180     418    3032    2179    6313

  Fragments arranged by size: 

                6313    3032    2490    2180    2179     418
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    6191    7332    9704    9908   11593   11651   12128   12193 
   Size:       5083    1141    2372     204    1685      58     477      65

Cuts at:  12193   12566    1108 
   Size:        373    5154

  Fragments arranged by size: 

                5154    5083    2372    1685    1141     477     373     204
                  65      58
 
PfoI T'CCnGG_A

Cuts at:   5876    7498    9697   12079   12754   13095    5876 
   Size:       1622    2199    2382     675     341    9393

  Fragments arranged by size: 

                9393    2382    2199    1622     675     341
 
PmeI GTTT'AAAC

Cuts at:  13916   13916 
   Size:      16612
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:  14681   14713   16194   16226   14681 
   Size:         32    1481      32   15067

  Fragments arranged by size: 

               15067    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   5198    7067    7496    8428   11372   12599    5198 
   Size:       1869     429     932    2944    1227    9211

  Fragments arranged by size: 

                9211    2944    1869    1227     932     429
 
PshAI GACnn'nnGTC

Cuts at:   1613    3044    3209    5724   10677   12574    1613 
   Size:       1431     165    2515    4953    1897    5651

  Fragments arranged by size: 

                5651    4953    2515    1897    1431     165
 
PsiI TTA'TAA

Cuts at:   4889    8354   14182    4889 
   Size:       3465    5828    7319

  Fragments arranged by size: 

                7319    5828    3465
 
PspOMI G'GGCC_C

Cuts at:   2132    2329   12434    2132 
   Size:        197   10105    6310

  Fragments arranged by size: 

               10105    6310     197
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:   8638    8670    8638 
   Size:         32   16580
 
PstI C_TGCA'G

Cuts at:    830    2355    3189    3723    4435    5222    5625    6895    6927 
   Size:       1525     834     534     712     787     403    1270      32

Cuts at:   6927    8500   11506   12829   13964     830 
   Size:       1573    3006    1323    1135    3478

  Fragments arranged by size: 

                3478    3006    1573    1525    1323    1270    1135     834
                 787     712     534     403      32
 
PvuI CG_AT'CG

Cuts at:   2001    5459    2001 
   Size:       3458   13154
 
PvuII CAG'CTG

Cuts at:   2016    3184    4868    5231    6415    6487    7795    8345    9986 
   Size:       1168    1684     363    1184      72    1308     550    1641

Cuts at:   9986   10705   11192   12515   12637   13204   13411    2016 
   Size:        719     487    1323     122     567     207    5217

  Fragments arranged by size: 

                5217    1684    1641    1323    1308    1184    1168     719
                 567     550     487     363     207     122      72
 
SacI G_AGCT'C

Cuts at:   4102    4305    8906    8951   10488    4102 
   Size:        203    4601      45    1537   10226

  Fragments arranged by size: 

               10226    4601    1537     203      45
 
SacII CC_GC'GG

Cuts at:   2099    2193    2099 
   Size:         94   16518
 
SalI G'TCGA_C

Cuts at:   1936    8481   13954    1936 
   Size:       6545    5473    4594

  Fragments arranged by size: 

                6545    5473    4594
 
SanDI GG'GwC_CC

Cuts at:  11372   12599   11372 
   Size:       1227   15385
 
SapI GCTCTTCn'nnn_

Cuts at:   5218    5218 
   Size:      16612
 
SbfI CC_TGCA'GG

Cuts at:  12829   13964   12829 
   Size:       1135   15477
 
ScaI AGT'ACT

Cuts at:   7555    8764   14860   16572    7555 
   Size:       1209    6096    1712    7595

  Fragments arranged by size: 

                7595    6096    1712    1209
 
SexAI A'CCwGG_T

Cuts at:   9349    9349 
   Size:      16612
 
SfcI C'TryA_G

Cuts at:    826    1045    2351    3185    3719    4431    5218    5621    5937 
   Size:        219    1306     834     534     712     787     403     316

Cuts at:   5937    6891    6923    8496    8820    9946   10902   11502   11681 
   Size:        954      32    1573     324    1126     956     600     179

Cuts at:  11681   12825   13504   13960   15095   15776   15967     826 
   Size:       1144     679     456    1135     681     191    1471

  Fragments arranged by size: 

                1573    1471    1306    1144    1135    1126     956     954
                 834     787     712     681     679     600     534     456
                 403     324     316     219     191     179      32
 
SfoI GGC'GCC

Cuts at:   2075    2092    2163   13885    2075 
   Size:         17      71   11722    4802

  Fragments arranged by size: 

               11722    4802      71      17
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:      16612
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:      16612
 
SmaI CCC'GGG

Cuts at:   1872    2044    2131    2158    2334   13939    1872 
   Size:        172      87      27     176   11605    4545

  Fragments arranged by size: 

               11605    4545     176     172      87      27
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2846    8020   10015   11657   14716   15617 
   Size:        197     926     903    5174    1995    1642    3059     901

Cuts at:  15617   15879     820 
   Size:        262    1553

  Fragments arranged by size: 

                5174    3059    1995    1642    1553     926     903     901
                 262     197
 
SnaBI TAC'GTA

Cuts at:    493   14403     493 
   Size:      13910    2702
 
SpeI A'CTAG_T

Cuts at:    152    2513    4370    4463    8765   10187     152 
   Size:       2361    1857      93    4302    1422    6577

  Fragments arranged by size: 

                6577    4302    2361    1857    1422      93
 
SphI G_CATG'C

Cuts at:   1132   13970    1132 
   Size:      12838    3774
 
SrfI GCCC'GGGC

Cuts at:   2334    2334 
   Size:      16612
 
SspI AAT'ATT

Cuts at:      5      52    3220    3945    4137   13762   14536       5 
   Size:         47    3168     725     192    9625     774    2081

  Fragments arranged by size: 

                9625    3168    2081     774     725     192      47
 
StuI AGG'CCT

Cuts at:   1517    2920    9477   11553    1517 
   Size:       1403    6557    2076    6576

  Fragments arranged by size: 

                6576    6557    2076    1403
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2083    3752    4451    4514    5239    5294 
   Size:        552     939      79    1669     699      63     725      55

Cuts at:   5294    5325    7153    7304    9531   10392   10990   11376   12104 
   Size:         31    1828     151    2227     861     598     386     728

Cuts at:  12104   13519   14051     513 
   Size:       1415     532    3074

  Fragments arranged by size: 

                3074    2227    1828    1669    1415     939     861     728
                 725     699     598     552     532     386     151      79
                  63      55      31
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2571    5401    6307    8388    8461    9523   10583 
   Size:       1255     991    2830     906    2081      73    1062    1060

Cuts at:  10583   11353   14639   14656   14809   14994   15439     325 
   Size:        770    3286      17     153     185     445    1498

  Fragments arranged by size: 

                3286    2830    2081    1498    1255    1062    1060     991
                 906     770     445     185     153      73      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    5513    6875    7553 
   Size:        274      80      33      51    1443    3536    1362     678

Cuts at:   7553    8762    9781   10561   14858   16570      96 
   Size:       1209    1019     780    4297    1712     138

  Fragments arranged by size: 

                4297    3536    1712    1443    1362    1209    1019     780
                 678     274     138      80      51      33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2482    2514    3183    3215    4264    4296    5901    5933    6410 
   Size:         32     669      32    1049      32    1605      32     477

Cuts at:   6410    6442    6632    6664    7815    7847   10112   10144   10165 
   Size:         32     190      32    1151      32    2265      32      21

Cuts at:  10165   10197   10801   10833   11477   11509   12659   12691   12736 
   Size:         32     604      32     644      32    1150      32      45

Cuts at:  12736   12768   16572   16604    2482 
   Size:         32    3804      32    2490

  Fragments arranged by size: 

                3804    2490    2265    1605    1151    1150    1049     669
                 644     604     477     190      45      32      32      32
                  32      32      32      32      32      32      32      32
                  32      32      32      21
 
Tth111I GACn'n_nGTC

Cuts at:   1316    4417    1316 
   Size:       3101   13511
 
XbaI T'CTAG_A

Cuts at:   3623    4130    7126    9023   10777   12643   13948    3623 
   Size:        507    2996    1897    1754    1866    1305    6287

  Fragments arranged by size: 

                6287    2996    1897    1866    1754    1305     507
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   6633    8069   10588   12812   12945    6633 
   Size:       1436    2519    2224     133   10300

  Fragments arranged by size: 

               10300    2519    2224    1436     133
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:      16612
 
XmaI C'CCGG_G

Cuts at:   1870    2042    2129    2156    2332   13937    1870 
   Size:        172      87      27     176   11605    4545

  Fragments arranged by size: 

               11605    4545     176     172      87      27
 
XmnI GAAnn'nnTTC

Cuts at:   3595    4183    7959    9936   14741    3595 
   Size:        588    3776    1977    4805    5466

  Fragments arranged by size: 

                5466    4805    3776    1977     588
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    5453   14420     276 
   Size:         53      83     186     936    3919    8967    2468

  Fragments arranged by size: 

                8967    3919    2468     936     186      83      53
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI
     ApoI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I
   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    HaeII    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NsiI     NspI     PasI     PciI    PflMI     PfoI
     PmeI     PpiI    PpuMI    PshAI     PsiI   PspOMI     PsrI     PstI
     PvuI    PvuII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI

 Enzymes that do not cut: 

     AloI     AscI    BsiWI    FspAI     NruI     PacI     PmlI    PspXI
    RsrII     SfiI     SwaI
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