(Circular) (Six-base) MAPSORT of: pFN21AE9396.seq Check: 7999 from: 1 to: 16612 pFN21AE9396 16612 bp With 182 enzymes: SgfI * May 26, 2016 14:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 13130 13547 13130 Size: 417 16195 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5455 14422 278 Size: 53 83 186 936 3919 8967 2468 Fragments arranged by size: 8967 3919 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3338 7042 8166 8482 10846 11680 12465 13503 Size: 1401 3704 1124 316 2364 834 785 1038 Cuts at: 13503 13955 1937 Size: 452 4594 Fragments arranged by size: 4594 3704 2364 1401 1124 1038 834 785 452 316 Acc65I G'GTAC_C Cuts at: 4016 11101 12116 13933 4016 Size: 7085 1015 1817 6695 Fragments arranged by size: 7085 6695 1817 1015 AclI AA'CG_TT Cuts at: 1563 13911 14739 15112 1563 Size: 12348 828 373 3063 Fragments arranged by size: 12348 3063 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2289 2487 3408 3546 4011 4173 6956 8844 Size: 657 198 921 138 465 162 2783 1888 Cuts at: 8844 8868 9054 9216 10008 10059 10299 10527 10980 Size: 24 186 162 792 51 240 228 453 Cuts at: 10980 11081 11237 11346 11532 12344 12414 12573 12720 Size: 101 156 109 186 812 70 159 147 Cuts at: 12720 12938 13323 13433 14675 16059 16327 1632 Size: 218 385 110 1242 1384 268 1917 Fragments arranged by size: 2783 1917 1888 1384 1242 921 812 792 657 465 453 385 268 240 228 218 198 186 186 162 162 159 156 147 138 110 109 101 70 51 24 AfeI AGC'GCT Cuts at: 8195 8195 Size: 16612 AflII C'TTAA_G Cuts at: 820 1017 10015 820 Size: 197 8998 7417 Fragments arranged by size: 8998 7417 197 AflIII A'CryG_T Cuts at: 1203 2104 5212 7294 7702 9882 10300 12085 13332 Size: 901 3108 2082 408 2180 418 1785 1247 Cuts at: 13332 15511 16488 1203 Size: 2179 977 1327 Fragments arranged by size: 3108 2180 2179 2082 1785 1327 1247 977 901 418 408 AgeI A'CCGG_T Cuts at: 15443 15443 Size: 16612 AhdI GACnn_n'nnGTC Cuts at: 8893 15341 8893 Size: 6448 10164 AleI CACnn'nnGTG Cuts at: 1166 6456 6866 7169 13728 1166 Size: 5290 410 303 6559 4050 Fragments arranged by size: 6559 5290 4050 410 303 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2396 4482 6138 8895 11477 12302 13460 13719 Size: 1212 2086 1656 2757 2582 825 1158 259 Cuts at: 13719 13883 1184 Size: 164 3913 Fragments arranged by size: 3913 2757 2582 2086 1656 1212 1158 825 259 164 AlwNI CAG_nnn'CTG Cuts at: 6490 9689 10705 10721 11228 11993 12172 12767 14355 Size: 3199 1016 16 507 765 179 595 1588 Cuts at: 14355 15927 6490 Size: 1572 7175 Fragments arranged by size: 7175 3199 1588 1572 1016 765 595 507 179 16 ApaI G_GGCC'C Cuts at: 2136 2333 12438 2136 Size: 197 10105 6310 Fragments arranged by size: 10105 6310 197 ApaLI G'TGCA_C Cuts at: 9870 14669 15825 9870 Size: 4799 1156 10657 Fragments arranged by size: 10657 4799 1156 ApoI r'AATT_y Cuts at: 1493 2560 4698 6180 6443 6961 7805 8100 8755 Size: 1067 2138 1482 263 518 844 295 655 Cuts at: 8755 8797 8985 10459 13020 13235 13921 14147 14299 Size: 42 188 1474 2561 215 686 226 152 Cuts at: 14299 16408 1493 Size: 2109 1697 Fragments arranged by size: 2561 2138 2109 1697 1482 1474 1067 844 686 655 518 295 263 226 215 188 152 42 AseI AT'TA_AT Cuts at: 160 1032 2964 7218 15166 160 Size: 872 1932 4254 7948 1606 Fragments arranged by size: 7948 4254 1932 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 16612 AvaI C'yCGr_G Cuts at: 1870 1943 2042 2129 2156 2332 3977 4568 7025 Size: 73 99 87 27 176 1645 591 2457 Cuts at: 7025 7881 13749 13937 1870 Size: 856 5868 188 4545 Fragments arranged by size: 5868 4545 2457 1645 856 591 188 176 99 87 73 27 AvrII C'CTAG_G Cuts at: 2083 2083 Size: 16612 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 9183 9216 10176 10209 10426 10459 11611 11644 9183 Size: 33 960 33 217 33 1152 33 14151 Fragments arranged by size: 14151 1152 960 217 33 33 33 33 BamHI G'GATC_C Cuts at: 5755 6614 11581 13942 14309 5755 Size: 859 4967 2361 367 8058 Fragments arranged by size: 8058 4967 2361 859 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2073 2090 2161 2372 4016 Size: 325 217 627 286 17 71 211 1644 Cuts at: 4016 5422 5491 6885 9544 11101 11899 12116 13211 Size: 1406 69 1394 2659 1557 798 217 1095 Cuts at: 13211 13883 13933 15388 618 Size: 672 50 1455 1842 Fragments arranged by size: 2659 1842 1644 1557 1455 1406 1394 1095 798 672 627 325 286 217 217 211 71 69 50 17 BanII G_rGCy'C Cuts at: 1391 2136 2333 3154 4088 4102 4305 5985 6366 Size: 745 197 821 934 14 203 1680 381 Cuts at: 6366 8906 8951 10488 10604 10940 12438 13931 1391 Size: 2540 45 1537 116 336 1498 1493 4072 Fragments arranged by size: 4072 2540 1680 1537 1498 1493 934 821 745 381 336 203 197 116 45 14 BbeI G_GCGC'C Cuts at: 2077 2094 2165 13887 2077 Size: 17 71 11722 4802 Fragments arranged by size: 11722 4802 71 17 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2268 2274 2680 3209 3414 4317 4803 Size: 971 369 6 406 529 205 903 486 Cuts at: 4803 5313 7578 7614 8930 9939 10038 928 Size: 510 2265 36 1316 1009 99 7502 Fragments arranged by size: 7502 2265 1316 1009 971 903 529 510 486 406 369 205 99 36 6 BbvCI CC'TCA_GC Cuts at: 2248 2296 3904 5925 6143 12511 2248 Size: 48 1608 2021 218 6368 6349 Fragments arranged by size: 6368 6349 2021 1608 218 48 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2000 2034 2241 2275 14803 14837 1903 Size: 34 63 34 207 34 12528 34 3678 Fragments arranged by size: 12528 3678 207 63 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4207 4731 4852 6071 9861 11962 12046 12454 12772 Size: 524 121 1219 3790 2101 84 408 318 Cuts at: 12772 14504 15714 4207 Size: 1732 1210 5105 Fragments arranged by size: 5105 3790 2101 1732 1219 1210 524 408 318 121 84 BclI T'GATC_A Cuts at: 1548 7108 7779 7944 8734 10978 1548 Size: 5560 671 165 790 2244 7182 Fragments arranged by size: 7182 5560 2244 790 671 165 BfrBI ATG'CAT Cuts at: 13696 13696 Size: 16612 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2088 2195 11942 Size: 107 122 71 631 605 416 107 9747 Cuts at: 11942 15223 136 Size: 3281 1525 Fragments arranged by size: 9747 3281 1525 631 605 416 122 107 107 71 BglII A'GATC_T Cuts at: 4250 7123 11867 13044 13803 16607 4250 Size: 2873 4744 1177 759 2804 4255 Fragments arranged by size: 4744 4255 2873 2804 1177 759 BlpI GC'TnA_GC Cuts at: 3120 3180 5100 10401 10406 10934 12768 13291 14029 Size: 60 1920 5301 5 528 1834 523 738 Cuts at: 14029 3120 Size: 5703 Fragments arranged by size: 5703 5301 1920 1834 738 528 523 60 5 Bme1580I G_kGCm'C Cuts at: 1790 2136 2333 3364 3661 9868 9874 11148 12438 Size: 346 197 1031 297 6207 6 1274 1290 Cuts at: 12438 14673 15829 1790 Size: 2235 1156 2573 Fragments arranged by size: 6207 2573 2235 1290 1274 1156 1031 346 297 197 6 BmgBI CAC'GTC Cuts at: 1318 12088 1318 Size: 10770 5842 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3444 3547 8442 Size: 416 53 83 399 24 2027 103 4895 Cuts at: 8442 8658 9969 10836 11334 12239 12402 14343 15301 Size: 216 1311 867 498 905 163 1941 958 Cuts at: 15301 442 Size: 1753 Fragments arranged by size: 4895 2027 1941 1753 1311 958 905 867 498 416 399 216 163 103 83 53 24 BmtI G_CTAG'C Cuts at: 1056 11454 12162 12789 1056 Size: 10398 708 627 4879 Fragments arranged by size: 10398 4879 708 627 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4144 9115 9850 11405 12817 13838 4144 Size: 4971 735 1555 1412 1021 6918 Fragments arranged by size: 6918 4971 1555 1412 1021 735 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2643 2712 3715 4973 5421 5853 6425 6512 Size: 1404 69 1003 1258 448 432 572 87 Cuts at: 6512 6609 6996 7521 8191 8217 8563 9165 12509 Size: 97 387 525 670 26 346 602 3344 Cuts at: 12509 12738 15272 1239 Size: 229 2534 2579 Fragments arranged by size: 3344 2579 2534 1404 1258 1003 670 602 572 525 448 432 387 346 229 97 87 69 26 Bpu10I CC'TnA_GC Cuts at: 2248 2296 3778 3904 5037 5925 6143 6262 8095 Size: 48 1482 126 1133 888 218 119 1833 Cuts at: 8095 12511 2248 Size: 4416 6349 Fragments arranged by size: 6349 4416 1833 1482 1133 888 218 126 119 48 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2831 8041 11642 14737 15602 15900 2831 Size: 5210 3601 3095 865 298 3543 Fragments arranged by size: 5210 3601 3543 3095 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2362 2502 2530 2606 2960 4441 4626 Size: 617 863 140 28 76 354 1481 185 Cuts at: 4626 5177 5902 6657 9135 11393 13515 15275 882 Size: 551 725 755 2478 2258 2122 1760 2219 Fragments arranged by size: 2478 2258 2219 2122 1760 1481 863 755 725 617 551 354 185 140 76 28 BsaAI yAC'GTr Cuts at: 493 1204 14403 493 Size: 711 13199 2702 Fragments arranged by size: 13199 2702 711 BsaBI GATnn'nnATC Cuts at: 8854 11872 16606 8854 Size: 3018 4734 8860 Fragments arranged by size: 8860 4734 3018 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2074 2091 2162 5452 Size: 53 83 186 936 541 17 71 3290 Cuts at: 5452 8584 13884 14419 14801 15436 275 Size: 3132 5300 535 382 635 1451 Fragments arranged by size: 5300 3290 3132 1451 936 635 541 535 382 186 83 71 53 17 BsaWI w'CCGG_w Cuts at: 2994 6103 8625 11578 12847 15045 15443 15717 15864 Size: 3109 2522 2953 1269 2198 398 274 147 Cuts at: 15864 16275 2994 Size: 411 3331 Fragments arranged by size: 3331 3109 2953 2522 2198 1269 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2482 2512 4453 4483 7815 Size: 30 165 30 1079 30 1941 30 3332 Cuts at: 7815 7845 10112 10142 12736 12766 1178 Size: 30 2267 30 2594 30 5024 Fragments arranged by size: 5024 3332 2594 2267 1941 1079 165 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2248 2832 2948 3247 3400 3421 3500 3521 Size: 1062 584 116 299 153 21 79 21 Cuts at: 3521 3667 4494 4586 5778 6401 7049 7512 8370 Size: 146 827 92 1192 623 648 463 858 Cuts at: 8370 8545 8896 9166 10133 10900 11565 13271 13412 Size: 175 351 270 967 767 665 1706 141 Cuts at: 13412 13415 13827 1186 Size: 3 412 3971 Fragments arranged by size: 3971 1706 1192 1062 967 858 827 767 665 648 623 584 463 412 351 299 270 175 153 146 141 116 92 79 21 21 3 BseYI C'CCAG_C Cuts at: 2219 3663 5901 6783 8610 10286 10829 10924 11784 Size: 1444 2238 882 1827 1676 543 95 860 Cuts at: 11784 12408 13162 15815 16311 2219 Size: 624 754 2653 496 2520 Fragments arranged by size: 2653 2520 2238 1827 1676 1444 882 860 754 624 543 496 95 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3414 7837 8946 12912 13071 13692 1163 Size: 573 1678 4423 1109 3966 159 621 4083 Fragments arranged by size: 4423 4083 3966 1678 1109 621 573 159 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2142 2190 2241 2364 5459 8486 Size: 275 186 141 48 51 123 3095 3027 Cuts at: 8486 14062 14823 15851 1540 Size: 5576 761 1028 2301 Fragments arranged by size: 5576 3095 3027 2301 1028 761 275 186 141 123 51 48 BsiHKAI G_wGCw'C Cuts at: 4102 4305 4471 8177 8906 8951 9874 10488 12291 Size: 203 166 3706 729 45 923 614 1803 Cuts at: 12291 14673 14758 15829 4102 Size: 2382 85 1071 4885 Fragments arranged by size: 4885 3706 2382 1803 1071 923 729 614 203 166 85 45 BsmI GAATG_Cn' Cuts at: 3874 6127 9325 14123 14216 3874 Size: 2253 3198 4798 93 6270 Fragments arranged by size: 6270 4798 3198 2253 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3687 11178 3687 Size: 7491 9121 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2136 2333 3154 3364 3661 4088 4102 Size: 399 346 197 821 210 297 427 14 Cuts at: 4102 4305 4471 5985 6366 8177 8906 8951 9868 Size: 203 166 1514 381 1811 729 45 917 Cuts at: 9868 9874 10488 10604 10940 11148 12291 12438 13931 Size: 6 614 116 336 208 1143 147 1493 Cuts at: 13931 14673 14758 15829 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1811 1514 1493 1143 1071 917 821 742 729 614 427 399 381 346 336 297 210 208 203 197 166 147 116 85 45 14 6 BspEI T'CCGG_A Cuts at: 2994 8625 11578 12847 16275 2994 Size: 5631 2953 1269 3428 3331 Fragments arranged by size: 5631 3428 3331 2953 1269 BspHI T'CATG_A Cuts at: 6655 6673 8521 14501 16231 6655 Size: 18 1848 5980 1730 7036 Fragments arranged by size: 7036 5980 1848 1730 18 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2344 2388 2819 5628 5884 12832 13130 13547 Size: 1500 44 431 2809 256 6948 298 417 Cuts at: 13547 13967 844 Size: 420 3489 Fragments arranged by size: 6948 3489 2809 1500 431 420 417 298 256 44 BsrBI CCG'CTC Cuts at: 1394 1823 2237 2412 3511 6883 13596 14499 1394 Size: 429 414 175 1099 3372 6713 903 3507 Fragments arranged by size: 6713 3507 3372 1099 903 429 414 175 BsrDI GCAATG_nn' Cuts at: 65 1248 3114 6770 8286 11730 15107 15281 65 Size: 1183 1866 3656 1516 3444 3377 174 1396 Fragments arranged by size: 3656 3444 3377 1866 1516 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2311 2385 6993 11896 13580 15256 15443 Size: 110 501 74 4608 4903 1684 1676 187 Cuts at: 15443 1700 Size: 2869 Fragments arranged by size: 4903 4608 2869 1684 1676 501 187 110 74 BsrGI T'GTAC_A Cuts at: 96 96 Size: 16612 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 16612 BssSI C'ACGA_G Cuts at: 2529 13726 14672 15684 2529 Size: 11197 946 1012 3457 Fragments arranged by size: 11197 3457 1012 946 BstAPI GCAn_nnn'nTGC Cuts at: 794 3116 3914 5234 13647 794 Size: 2322 798 1320 8413 3759 Fragments arranged by size: 8413 3759 2322 1320 798 BstBI TT'CG_AA Cuts at: 8440 8566 15416 8440 Size: 126 6850 9636 Fragments arranged by size: 9636 6850 126 BstEII G'GTnAC_C Cuts at: 1184 12859 1184 Size: 11675 4937 BstXI CCAn_nnnn'nTGG Cuts at: 7261 11347 7261 Size: 4086 12526 BstYI r'GATC_y Cuts at: 1972 2578 3374 3620 4250 5755 6579 6614 7123 Size: 606 796 246 630 1505 824 35 509 Cuts at: 7123 8468 8849 8957 9260 9524 9917 10948 11581 Size: 1345 381 108 303 264 393 1031 633 Cuts at: 11581 11867 13044 13441 13803 13942 14309 14694 14711 Size: 286 1177 397 362 139 367 385 17 Cuts at: 14711 16163 16249 16261 16607 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1505 1452 1345 1177 1031 824 796 633 630 606 509 397 393 385 381 367 362 346 303 286 264 246 139 108 86 35 17 12 BstZ17I GTA'TAC Cuts at: 7043 8167 7043 Size: 1124 15488 Bsu36I CC'TnA_GG Cuts at: 3504 5496 9822 12739 13313 3504 Size: 1992 4326 2917 574 6803 Fragments arranged by size: 6803 4326 2917 1992 574 BtgI C'CryG_G Cuts at: 513 1065 2004 2096 2190 5294 5325 7304 7830 Size: 552 939 92 94 3104 31 1979 526 Cuts at: 7830 9548 11376 13519 513 Size: 1718 1828 2143 3606 Fragments arranged by size: 3606 3104 2143 1979 1828 1718 939 552 526 94 92 31 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 15873 Fragments arranged by size: 15873 683 56 BtsI GCAGTG_nn' Cuts at: 3539 3716 3804 5480 5570 5704 7386 9868 10928 Size: 177 88 1676 90 134 1682 2482 1060 Cuts at: 10928 14128 14922 14942 3539 Size: 3200 794 20 5209 Fragments arranged by size: 5209 3200 2482 1682 1676 1060 794 177 134 90 88 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 16612 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3734 3769 5524 5559 6359 6394 7530 Size: 35 3170 35 1755 35 800 35 1136 Cuts at: 7530 7565 7676 7711 529 Size: 35 111 35 9430 Fragments arranged by size: 9430 3170 1755 1136 800 111 35 35 35 35 35 35 DraI TTT'AAA Cuts at: 3870 8913 9841 13916 3870 Size: 5043 928 4075 6566 Fragments arranged by size: 6566 5043 4075 928 DraIII CAC_nnn'GTG Cuts at: 11342 11918 11342 Size: 576 16036 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3679 15619 809 Size: 507 2363 11940 1802 Fragments arranged by size: 11940 2363 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2187 2238 2361 Size: 54 1425 50 275 18 357 51 123 Cuts at: 2361 5242 7684 10321 13522 14059 14948 16577 8 Size: 2881 2442 2637 3201 537 889 1629 43 Fragments arranged by size: 3201 2881 2637 2442 1629 1425 889 537 357 275 123 54 51 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2187 2238 2361 14059 1537 Size: 275 375 51 123 11698 4090 Fragments arranged by size: 11698 4090 375 275 123 51 EarI CTCTTCn'nnn_ Cuts at: 1353 2308 2660 4095 4339 4514 5114 5218 7984 Size: 955 352 1435 244 175 600 104 2766 Cuts at: 7984 8602 8797 9073 10744 10871 11289 11326 12909 Size: 618 195 276 1671 127 418 37 1583 Cuts at: 12909 13389 14542 1353 Size: 480 1153 3423 Fragments arranged by size: 3423 2766 1671 1583 1435 1153 955 618 600 480 418 352 276 244 195 175 127 104 37 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2257 4816 15199 15573 15719 387 Size: 1064 138 668 2559 10383 374 146 1280 Fragments arranged by size: 10383 2559 1280 1064 668 374 146 138 EcoICRI GAG'CTC Cuts at: 4100 4303 8904 8949 10486 4100 Size: 203 4601 45 1537 10226 Fragments arranged by size: 10226 4601 1537 203 45 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2289 2487 2643 2712 3408 3546 3715 Size: 393 657 198 156 69 696 138 169 Cuts at: 3715 4011 4173 4973 5421 5853 6425 6512 6609 Size: 296 162 800 448 432 572 87 97 Cuts at: 6609 6956 6996 7521 8191 8217 8563 8844 8868 Size: 347 40 525 670 26 346 281 24 Cuts at: 8868 9054 9165 9216 10008 10059 10299 10527 10980 Size: 186 111 51 792 51 240 228 453 Cuts at: 10980 11081 11237 11346 11532 12344 12414 12509 12573 Size: 101 156 109 186 812 70 95 64 Cuts at: 12573 12720 12738 12938 13323 13433 14675 15272 16059 Size: 147 18 200 385 110 1242 597 787 Cuts at: 16059 16327 1239 Size: 268 1524 Fragments arranged by size: 1524 1242 812 800 792 787 696 670 657 597 572 525 453 448 432 393 385 347 346 296 281 268 240 228 200 198 186 186 169 162 156 156 147 138 111 110 109 101 97 95 87 70 69 64 51 51 40 26 24 18 EcoNI CCTnn'n_nnAGG Cuts at: 3816 4955 5087 5937 8496 11547 3816 Size: 1139 132 850 2559 3051 8881 Fragments arranged by size: 8881 3051 2559 1139 850 132 EcoO109I rG'GnC_Cy Cuts at: 2080 2133 4564 5198 7067 7496 8428 9094 11372 Size: 53 2431 634 1869 429 932 666 2278 Cuts at: 11372 12434 12435 12599 2080 Size: 1062 1 164 6093 Fragments arranged by size: 6093 2431 2278 1869 1062 932 666 634 429 164 53 1 EcoRI G'AATT_C Cuts at: 6961 7805 8100 8755 8985 13921 14299 6961 Size: 844 295 655 230 4936 378 9274 Fragments arranged by size: 9274 4936 844 655 378 295 230 EcoRV GAT'ATC Cuts at: 8150 8251 8150 Size: 101 16511 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2517 2799 3145 3414 3944 4272 7291 2517 Size: 282 346 269 530 328 3019 11838 Fragments arranged by size: 11838 3019 530 346 328 282 269 FseI GG_CCGG'CC Cuts at: 2315 2315 Size: 16612 FspI TGC'GCA Cuts at: 15118 15118 Size: 16612 HaeII r_GCGC'y Cuts at: 1678 2029 2077 2094 2165 2351 6400 8197 13887 Size: 351 48 17 71 186 4049 1797 5690 Cuts at: 13887 15759 1678 Size: 1872 2531 Fragments arranged by size: 5690 4049 2531 1872 1797 351 186 71 48 17 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2603 2616 2635 2648 2685 Size: 32 135 32 1031 13 19 13 37 Cuts at: 2685 2717 3216 3248 4453 4485 4562 4594 4891 Size: 32 499 32 1205 32 77 32 297 Cuts at: 4891 4923 5113 5145 5327 5330 5359 5362 5569 Size: 32 190 32 182 3 29 3 207 Cuts at: 5569 5601 5645 5677 6818 6850 7736 7768 8620 Size: 32 44 32 1141 32 886 32 852 Cuts at: 8620 8652 8699 8731 8879 8911 9590 9622 9647 Size: 32 47 32 148 32 679 32 25 Cuts at: 9647 9679 10772 10804 10872 10904 10963 10995 11000 Size: 32 1093 32 68 32 59 32 5 Cuts at: 11000 11032 11804 11836 12116 12148 12853 12885 13190 Size: 32 772 32 280 32 705 32 305 Cuts at: 13190 13222 15253 15285 15327 15359 1373 Size: 32 2031 32 42 32 2626 Fragments arranged by size: 2626 2031 1205 1141 1093 1031 886 852 772 705 679 499 305 297 280 207 190 182 148 135 77 68 59 47 44 42 37 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 29 25 19 13 13 5 3 3 HincII GTy'rAC Cuts at: 669 1657 1938 8483 9660 11248 13147 13956 14202 Size: 988 281 6545 1177 1588 1899 809 246 Cuts at: 14202 669 Size: 3079 Fragments arranged by size: 6545 3079 1899 1588 1177 988 809 281 246 HindIII A'AGCT_T Cuts at: 748 2870 5048 11416 748 Size: 2122 2178 6368 5944 Fragments arranged by size: 6368 5944 2178 2122 HpaI GTT'AAC Cuts at: 11248 13147 14202 11248 Size: 1899 1055 13658 Fragments arranged by size: 13658 1899 1055 KasI G'GCGC_C Cuts at: 2073 2090 2161 13883 2073 Size: 17 71 11722 4802 Fragments arranged by size: 11722 4802 71 17 KpnI G_GTAC'C Cuts at: 4020 11105 12120 13937 4020 Size: 7085 1015 1817 6695 Fragments arranged by size: 7085 6695 1817 1015 MfeI C'AATT_G Cuts at: 5349 11312 14211 5349 Size: 5963 2899 7750 Fragments arranged by size: 7750 5963 2899 MluI A'CGCG_T Cuts at: 2104 16488 2104 Size: 14384 2228 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3585 3791 3982 7155 8142 8324 8483 10134 Size: 2280 206 191 3173 987 182 159 1651 Cuts at: 10134 10476 10976 12471 15726 15910 1305 Size: 342 500 1495 3255 184 2007 Fragments arranged by size: 3255 3173 2280 2007 1651 1495 987 500 342 206 191 184 182 159 MscI TGG'CCA Cuts at: 10 64 1489 1832 7686 10323 10 Size: 54 1425 343 5854 2637 6299 Fragments arranged by size: 6299 5854 2637 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3856 6456 6866 7169 7407 8844 10329 Size: 648 2690 2600 410 303 238 1437 1485 Cuts at: 10329 11001 12284 13701 13728 14570 14929 15088 518 Size: 672 1283 1417 27 842 359 159 2042 Fragments arranged by size: 2690 2600 2042 1485 1437 1417 1283 842 672 648 410 359 303 238 159 27 MspA1I CmG'CkG Cuts at: 1606 2016 2098 2192 2214 2430 3184 4022 4868 Size: 410 82 94 22 216 754 838 846 Cuts at: 4868 5231 6415 6487 7727 7795 8345 8614 9986 Size: 363 1184 72 1240 68 550 269 1372 Cuts at: 9986 10705 11192 11942 12269 12515 12574 12637 13204 Size: 719 487 750 327 246 59 63 567 Cuts at: 13204 13411 14028 14705 15853 16098 1606 Size: 207 617 677 1148 245 2120 Fragments arranged by size: 2120 1372 1240 1184 1148 846 838 754 750 719 677 617 567 550 487 410 363 327 269 246 245 216 207 94 82 72 68 63 59 22 NaeI GCC'GGC Cuts at: 2313 13582 2313 Size: 11269 5343 NarI GG'CG_CC Cuts at: 2074 2091 2162 13884 2074 Size: 17 71 11722 4802 Fragments arranged by size: 11722 4802 71 17 NcoI C'CATG_G Cuts at: 513 1065 2004 5294 5325 7304 11376 13519 513 Size: 552 939 3290 31 1979 4072 2143 3606 Fragments arranged by size: 4072 3606 3290 2143 1979 939 552 31 NdeI CA'TA_TG Cuts at: 387 7770 9255 387 Size: 7383 1485 7744 Fragments arranged by size: 7744 7383 1485 NgoMIV G'CCGG_C Cuts at: 2311 13580 2311 Size: 11269 5343 NheI G'CTAG_C Cuts at: 1052 11450 12158 12785 1052 Size: 10398 708 627 4879 Fragments arranged by size: 10398 4879 708 627 NotI GC'GGCC_GC Cuts at: 2187 2238 14059 2187 Size: 51 11821 4740 Fragments arranged by size: 11821 4740 51 NsiI A_TGCA'T Cuts at: 13698 13698 Size: 16612 NspI r_CATG'y Cuts at: 1132 1229 5216 5275 6332 7706 8211 9886 10304 Size: 97 3987 59 1057 1374 505 1675 418 Cuts at: 10304 11260 12368 13336 13970 15515 1132 Size: 956 1108 968 634 1545 2229 Fragments arranged by size: 3987 2229 1675 1545 1374 1108 1057 968 956 634 505 418 97 59 PasI CC'CwG_GG Cuts at: 1348 5840 6294 1348 Size: 4492 454 11666 Fragments arranged by size: 11666 4492 454 PciI A'CATG_T Cuts at: 5212 7702 9882 10300 13332 15511 5212 Size: 2490 2180 418 3032 2179 6313 Fragments arranged by size: 6313 3032 2490 2180 2179 418 PflMI CCAn_nnn'nTGG Cuts at: 1108 6191 7332 9704 9908 11593 11651 12128 12193 Size: 5083 1141 2372 204 1685 58 477 65 Cuts at: 12193 12566 1108 Size: 373 5154 Fragments arranged by size: 5154 5083 2372 1685 1141 477 373 204 65 58 PfoI T'CCnGG_A Cuts at: 5876 7498 9697 12079 12754 13095 5876 Size: 1622 2199 2382 675 341 9393 Fragments arranged by size: 9393 2382 2199 1622 675 341 PmeI GTTT'AAAC Cuts at: 13916 13916 Size: 16612 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 14681 14713 16194 16226 14681 Size: 32 1481 32 15067 Fragments arranged by size: 15067 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 5198 7067 7496 8428 11372 12599 5198 Size: 1869 429 932 2944 1227 9211 Fragments arranged by size: 9211 2944 1869 1227 932 429 PshAI GACnn'nnGTC Cuts at: 1613 3044 3209 5724 10677 12574 1613 Size: 1431 165 2515 4953 1897 5651 Fragments arranged by size: 5651 4953 2515 1897 1431 165 PsiI TTA'TAA Cuts at: 4889 8354 14182 4889 Size: 3465 5828 7319 Fragments arranged by size: 7319 5828 3465 PspOMI G'GGCC_C Cuts at: 2132 2329 12434 2132 Size: 197 10105 6310 Fragments arranged by size: 10105 6310 197 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 8638 8670 8638 Size: 32 16580 PstI C_TGCA'G Cuts at: 830 2355 3189 3723 4435 5222 5625 6895 6927 Size: 1525 834 534 712 787 403 1270 32 Cuts at: 6927 8500 11506 12829 13964 830 Size: 1573 3006 1323 1135 3478 Fragments arranged by size: 3478 3006 1573 1525 1323 1270 1135 834 787 712 534 403 32 PvuI CG_AT'CG Cuts at: 2001 5459 2001 Size: 3458 13154 PvuII CAG'CTG Cuts at: 2016 3184 4868 5231 6415 6487 7795 8345 9986 Size: 1168 1684 363 1184 72 1308 550 1641 Cuts at: 9986 10705 11192 12515 12637 13204 13411 2016 Size: 719 487 1323 122 567 207 5217 Fragments arranged by size: 5217 1684 1641 1323 1308 1184 1168 719 567 550 487 363 207 122 72 SacI G_AGCT'C Cuts at: 4102 4305 8906 8951 10488 4102 Size: 203 4601 45 1537 10226 Fragments arranged by size: 10226 4601 1537 203 45 SacII CC_GC'GG Cuts at: 2099 2193 2099 Size: 94 16518 SalI G'TCGA_C Cuts at: 1936 8481 13954 1936 Size: 6545 5473 4594 Fragments arranged by size: 6545 5473 4594 SanDI GG'GwC_CC Cuts at: 11372 12599 11372 Size: 1227 15385 SapI GCTCTTCn'nnn_ Cuts at: 5218 5218 Size: 16612 SbfI CC_TGCA'GG Cuts at: 12829 13964 12829 Size: 1135 15477 ScaI AGT'ACT Cuts at: 7555 8764 14860 16572 7555 Size: 1209 6096 1712 7595 Fragments arranged by size: 7595 6096 1712 1209 SexAI A'CCwGG_T Cuts at: 9349 9349 Size: 16612 SfcI C'TryA_G Cuts at: 826 1045 2351 3185 3719 4431 5218 5621 5937 Size: 219 1306 834 534 712 787 403 316 Cuts at: 5937 6891 6923 8496 8820 9946 10902 11502 11681 Size: 954 32 1573 324 1126 956 600 179 Cuts at: 11681 12825 13504 13960 15095 15776 15967 826 Size: 1144 679 456 1135 681 191 1471 Fragments arranged by size: 1573 1471 1306 1144 1135 1126 956 954 834 787 712 681 679 600 534 456 403 324 316 219 191 179 32 SfoI GGC'GCC Cuts at: 2075 2092 2163 13885 2075 Size: 17 71 11722 4802 Fragments arranged by size: 11722 4802 71 17 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 16612 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 16612 SmaI CCC'GGG Cuts at: 1872 2044 2131 2158 2334 13939 1872 Size: 172 87 27 176 11605 4545 Fragments arranged by size: 11605 4545 176 172 87 27 SmlI C'TyrA_G Cuts at: 820 1017 1943 2846 8020 10015 11657 14716 15617 Size: 197 926 903 5174 1995 1642 3059 901 Cuts at: 15617 15879 820 Size: 262 1553 Fragments arranged by size: 5174 3059 1995 1642 1553 926 903 901 262 197 SnaBI TAC'GTA Cuts at: 493 14403 493 Size: 13910 2702 SpeI A'CTAG_T Cuts at: 152 2513 4370 4463 8765 10187 152 Size: 2361 1857 93 4302 1422 6577 Fragments arranged by size: 6577 4302 2361 1857 1422 93 SphI G_CATG'C Cuts at: 1132 13970 1132 Size: 12838 3774 SrfI GCCC'GGGC Cuts at: 2334 2334 Size: 16612 SspI AAT'ATT Cuts at: 5 52 3220 3945 4137 13762 14536 5 Size: 47 3168 725 192 9625 774 2081 Fragments arranged by size: 9625 3168 2081 774 725 192 47 StuI AGG'CCT Cuts at: 1517 2920 9477 11553 1517 Size: 1403 6557 2076 6576 Fragments arranged by size: 6576 6557 2076 1403 StyI C'CwwG_G Cuts at: 513 1065 2004 2083 3752 4451 4514 5239 5294 Size: 552 939 79 1669 699 63 725 55 Cuts at: 5294 5325 7153 7304 9531 10392 10990 11376 12104 Size: 31 1828 151 2227 861 598 386 728 Cuts at: 12104 13519 14051 513 Size: 1415 532 3074 Fragments arranged by size: 3074 2227 1828 1669 1415 939 861 728 725 699 598 552 532 386 151 79 63 55 31 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2571 5401 6307 8388 8461 9523 10583 Size: 1255 991 2830 906 2081 73 1062 1060 Cuts at: 10583 11353 14639 14656 14809 14994 15439 325 Size: 770 3286 17 153 185 445 1498 Fragments arranged by size: 3286 2830 2081 1498 1255 1062 1060 991 906 770 445 185 153 73 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5513 6875 7553 Size: 274 80 33 51 1443 3536 1362 678 Cuts at: 7553 8762 9781 10561 14858 16570 96 Size: 1209 1019 780 4297 1712 138 Fragments arranged by size: 4297 3536 1712 1443 1362 1209 1019 780 678 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2482 2514 3183 3215 4264 4296 5901 5933 6410 Size: 32 669 32 1049 32 1605 32 477 Cuts at: 6410 6442 6632 6664 7815 7847 10112 10144 10165 Size: 32 190 32 1151 32 2265 32 21 Cuts at: 10165 10197 10801 10833 11477 11509 12659 12691 12736 Size: 32 604 32 644 32 1150 32 45 Cuts at: 12736 12768 16572 16604 2482 Size: 32 3804 32 2490 Fragments arranged by size: 3804 2490 2265 1605 1151 1150 1049 669 644 604 477 190 45 32 32 32 32 32 32 32 32 32 32 32 32 32 32 21 Tth111I GACn'n_nGTC Cuts at: 1316 4417 1316 Size: 3101 13511 XbaI T'CTAG_A Cuts at: 3623 4130 7126 9023 10777 12643 13948 3623 Size: 507 2996 1897 1754 1866 1305 6287 Fragments arranged by size: 6287 2996 1897 1866 1754 1305 507 XcmI CCAnnnn_n'nnnnTGG Cuts at: 6633 8069 10588 12812 12945 6633 Size: 1436 2519 2224 133 10300 Fragments arranged by size: 10300 2519 2224 1436 133 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 16612 XmaI C'CCGG_G Cuts at: 1870 2042 2129 2156 2332 13937 1870 Size: 172 87 27 176 11605 4545 Fragments arranged by size: 11605 4545 176 172 87 27 XmnI GAAnn'nnTTC Cuts at: 3595 4183 7959 9936 14741 3595 Size: 588 3776 1977 4805 5466 Fragments arranged by size: 5466 4805 3776 1977 588 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5453 14420 276 Size: 53 83 186 936 3919 8967 2468 Fragments arranged by size: 8967 3919 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI BsiWI FspAI NruI PacI PmlI PspXI RsrII SfiI SwaI