################################## # NO CUT ENZYMES ( 157 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AflII AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaI AvaII AviII AvrII BamHI BanI BanII BbeI BbrPI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I CelII Cfr10I Cfr13I ClaI CpoI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRV FauI FbaI FseI FspI HaeII HapII HindIII HpaII KasI KpnI KspI MaeI MaeII MamI MfeI MluI MroI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI Sau96I SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TaqI Tth111I TthHB8I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AccI 859 GTCTAC GT^[AC][GT]AC AciI 357 CCGC CCGC(-3/-1) AcyI 692 GACGCC G[GA]^CG[CT]C AluI 219 AGCT AG^CT AocI 867 CCTGAGG CC^T[ACGT]AGG AspHI 416 GTGCTC G[AT]GC[AT]^C BalI 425 TGGCCA TGG^CCA BbsI 82 GTCTTC GAAGAC(2/6) BbvCI 215 CCTCAGC CCTCAGC(-5/-2) BbvI 364 GCAGC GCAGC(8/12) BpmI 783 CTCCAG CTGGAG(16/14) BpuAI 82 GTCTTC GAAGAC(2/6) BsaHI 692 GACGCC G[GA]^CG[CT]C BsiHKAI 416 GTGCTC G[AT]GC[AT]^C BsmFI 751 GGGAC GGGAC(10/14) BsmI 456 GAATGC GAATGC(1/-1) BspHI 668 TCATGA T^CATGA BstXI 230 CCAAGGTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 867 CCTGAGG CC^T[ACGT]AGG Cac8I 418 GCTCGC GC[ACGT]^[ACGT]GC CfoI 678 GCGC GCG^C DraI 895 TTTAAA TTT^AAA EaeI 425 TGGCCA [CT]^GGCC[GA] EarI 73 CTCTTC CTCTTC(1/4) Eco81I 867 CCTGAGG CC^T[ACGT]AGG EcoRI 25 GAATTC G^AATTC EcoT14I 230 CCAAGG C^C[AT][AT]GG EcoT22I 458 ATGCAT ATGCA^T Fnu4HI 364 GCAGC GC^[ACGT]GC HgaI 692 GACGC GACGC(5/10) HhaI 678 GCGC GCG^C Hin1I 692 GACGCC G[GA]^CG[CT]C HinP1I 678 GCGC G^CGC HincII 42 GTTAAC GT[CT]^[GA]AC HindII 42 GTTAAC GT[CT]^[GA]AC HpaI 42 GTTAAC GTT^AAC ItaI 364 GCAGC GC^[ACGT]GC Ksp632I 73 CTCTTC CTCTTC(1/4) MluNI 425 TGGCCA TGG^CCA MscI 425 TGGCCA TGG^CCA MspA1I 218 CAGCTG C[AC]G^C[GT]G NsiI 458 ATGCAT ATGCA^T PflMI 789 CCATTTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 148 GACTCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 218 CAGCTG CAG^CTG RcaI 668 TCATGA T^CATGA StyI 230 CCAAGG C^C[AT][AT]GG TseI 364 GCAGC G^C[AT]GC Van91I 789 CCATTTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AcsI 25 GAATTC [GA]^AATT[CT] 262 AAATTC AfaI 171 GTAC GT^AC 768 GTAC AlwI 66 GATCC GGATC(4/5) 503 GATCC ApoI 25 GAATTC [GA]^AATT[CT] 262 AAATTC Asp700I 133 GAATTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 477 GAACACTTTC BmyI 416 GTGCTC G[AGT]GC[ACT]^C 798 GGGCAC BseRI 536 CTCCTC GAGGAG(10/8) 539 CTCCTC BsiYI 189 CCTCTCCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 789 CCATTTCATGG BslI 189 CCTCTCCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 789 CCATTTCATGG BsmAI 257 GAGAC GTCTC(1/5) 155 GTCTC Bsp1286I 416 GTGCTC G[AGT]GC[ACT]^C 798 GGGCAC BstYI 65 AGATCC [GA]^GATC[CT] 502 AGATCC MflI 65 AGATCC [GA]^GATC[CT] 502 AGATCC MwoI 340 GCATCAATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 418 GCTCGCCTGGC NlaIV 116 GGAACC GG[ACGT]^[ACGT]CC 433 GGCTCC PleI 148 GACTC GAGTC(4/5) 549 GACTC RsaI 171 GTAC GT^AC 768 GTAC StuI 701 AGGCCT AGG^CCT 920 AGGCCT Tsp45I 19 GTGAC ^GT[GC]AC 223 GTCAC XcmI 230 CCAAGGTGATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 785 CCAGCCATTTCATGG XhoII 65 AGATCC [GA]^GATC[CT] 502 AGATCC XmnI 133 GAATTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 477 GAACACTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 30 enzymes) # ######################################### BsaJI BsrI BssKI BstF5I BstNI DdeI DpnII EcoRII FokI HaeIII HinfI HphI MaeIII MboI MboII MnlI MseI MslI MvaI NdeII NlaIII PstI Sau3AI ScrFI SfaNI SfcI TfiI Tru9I Tsp509I TspRI