Restriction map for pFN21AE5993
 (Circular) (Six-base) MAPSORT of: pFN21AE5993.seq  Check: 1967  from: 1  to: 5647



pFN21AE5993  5647 bp

 With 182 enzymes: SgfI * 

                        December 8, 2014 13:49  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3457     278 
   Size:         53      83     186     936    1921    2468

  Fragments arranged by size: 

                2468    1921     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2865    2990    1937 
   Size:        928     125    4594

  Fragments arranged by size: 

                4594     928     125
 
Acc65I G'GTAC_C

Cuts at:   2968    2968 
   Size:       5647
 
AclI AA'CG_TT

Cuts at:   1563    3774    4147    1563 
   Size:       2211     373    3063

  Fragments arranged by size: 

                3063    2211     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2712    3710    5094    5362    1632 
   Size:       1080     998    1384     268    1917

  Fragments arranged by size: 

                1917    1384    1080     998     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5450
 
AflIII A'CryG_T

Cuts at:   1203    4546    5523    1203 
   Size:       3343     977    1327

  Fragments arranged by size: 

                3343    1327     977
 
AgeI A'CCGG_T

Cuts at:   4478    4478 
   Size:       5647
 
AhdI GACnn_n'nnGTC

Cuts at:   4376    4376 
   Size:       5647
 
AleI CACnn'nnGTG

Cuts at:   1166    2021    1166 
   Size:        855    4792
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5647
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2055    2087    2055 
   Size:         32    5615
 
AlwNI CAG_nnn'CTG

Cuts at:   3390    4962    3390 
   Size:       1572    4075
 
ApaLI G'TGCA_C

Cuts at:   3704    4860    3704 
   Size:       1156    4491
 
ApoI r'AATT_y

Cuts at:   1493    2030    2267    2956    3182    3334    5443    1493 
   Size:        537     237     689     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     689     537     237     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    4201     160 
   Size:        872    3169    1606

  Fragments arranged by size: 

                3169    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5647
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2972    1870 
   Size:         73    1029    4545

  Fragments arranged by size: 

                4545    1029      73
 
BamHI G'GATC_C

Cuts at:   2977    3344    2977 
   Size:        367    5280
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2968    4423     618 
   Size:        325     217     627    1181    1455    1842

  Fragments arranged by size: 

                1842    1455    1181     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2966    1391 
   Size:       1575    4072
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2080     928 
   Size:        971     181    4495

  Fragments arranged by size: 

                4495     971     181
 
BbvCI CC'TCA_GC

Cuts at:   2221    2221 
   Size:       5647
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3838    3872    1903 
   Size:         34    1901      34    3678

  Fragments arranged by size: 

                3678    1901      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3539    4749    3539 
   Size:       1210    4437
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5647
 
BfrBI ATG'CAT

Cuts at:   2465    2465 
   Size:       5647
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4258     136 
   Size:        107     122      71     631     605    2586    1525

  Fragments arranged by size: 

                2586    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   5642    5642 
   Size:       5647
 
BlpI GC'TnA_GC

Cuts at:   3064    3064 
   Size:       5647
 
Bme1580I G_kGCm'C

Cuts at:   1790    2807    3708    4864    1790 
   Size:       1017     901    1156    2573

  Fragments arranged by size: 

                2573    1156    1017     901
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5647
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2487    3378    4336 
   Size:        416      53      83     399      24    1070     891     958

Cuts at:   4336     442 
   Size:       1753

  Fragments arranged by size: 

                1753    1070     958     891     416     399      83      53
                  24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5647
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2773    4307    1239 
   Size:       1534    1534    2579

  Fragments arranged by size: 

                2579    1534    1534
 
Bpu10I CC'TnA_GC

Cuts at:   2221    2221 
   Size:       5647
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3772    4637    4935    3772 
   Size:        865     298    4484

  Fragments arranged by size: 

                4484     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4310     882 
   Size:        617    2811    2219

  Fragments arranged by size: 

                2811    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3438     493 
   Size:        711    2234    2702

  Fragments arranged by size: 

                2702    2234     711
 
BsaBI GATnn'nnATC

Cuts at:   5641    5641 
   Size:       5647
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2698    3454    3836    4471 
   Size:         53      83     186     936    1165     756     382     635

Cuts at:   4471     275 
   Size:       1451

  Fragments arranged by size: 

                1451    1165     936     756     635     382     186      83
                  53
 
BsaWI w'CCGG_w

Cuts at:   4080    4478    4752    4899    5310    4080 
   Size:        398     274     147     411    4417

  Fragments arranged by size: 

                4417     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    5422

  Fragments arranged by size: 

                5422     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2532    2535    1186 
   Size:       1346       3    4298

  Fragments arranged by size: 

                4298    1346       3
 
BseYI C'CCAG_C

Cuts at:   4850    5346    4850 
   Size:        496    5151
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    5074
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3097    3858    4886    1540 
   Size:        275     186    1096     761    1028    2301

  Fragments arranged by size: 

                2301    1096    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2425    3708    3793    4864    2425 
   Size:       1283      85    1071    3208

  Fragments arranged by size: 

                3208    1283    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2467    3158    3251    2467 
   Size:        691      93    4863

  Fragments arranged by size: 

                4863     691      93
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2425    2807    2966    3708    3793    4864    1391 
   Size:        399     635     382     159     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     635     399     382     159      85
            
 
BspEI T'CCGG_A

Cuts at:   5310    5310 
   Size:       5647
 
BspHI T'CATG_A

Cuts at:   2673    3536    5266    2673 
   Size:        863    1730    3054

  Fragments arranged by size: 

                3054    1730     863
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    3002     844 
   Size:       2158    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3534    1394 
   Size:        429    1711    3507

  Fragments arranged by size: 

                3507    1711     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    4142    4316      65 
   Size:       1183    2894     174    1396

  Fragments arranged by size: 

                2894    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4291    4478    1700 
   Size:        110    2481     187    2869

  Fragments arranged by size: 

                2869    2481     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5647
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5647
 
BssSI C'ACGA_G

Cuts at:   3707    4719    3707 
   Size:       1012    4635
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5647
 
BstBI TT'CG_AA

Cuts at:   4451    4451 
   Size:       5647
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5647
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2242    2242 
   Size:       5647
 
BstYI r'GATC_y

Cuts at:   1972    2070    2507    2977    3344    3729    3746    5198    5284 
   Size:         98     437     470     367     385      17    1452      86

Cuts at:   5284    5296    5642    1972 
   Size:         12     346    1977

  Fragments arranged by size: 

                1977    1452     470     437     385     367     346      98
                  86      17      12
 
Bsu36I CC'TnA_GG

Cuts at:   2873    2873 
   Size:       5647
 
BtgI C'CryG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    4156

  Fragments arranged by size: 

                4156     939     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4908

  Fragments arranged by size: 

                4908     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2322    3163    3957    3977    2322 
   Size:        841     794      20    3992

  Fragments arranged by size: 

                3992     841     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5647
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5612
 
DraI TTT'AAA

Cuts at:   2902    2951    2902 
   Size:         49    5598
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4654     809 
   Size:        507    3338    1802

  Fragments arranged by size: 

                3338    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2430    3094    3983 
   Size:         54    1425      50     275      18     600     664     889

Cuts at:   3983    5612       8 
   Size:       1629      43

  Fragments arranged by size: 

                1629    1425     889     664     600     275      54      50
                  43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3094    1537 
   Size:        275    1282    4090

  Fragments arranged by size: 

                4090    1282     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2084    3577    1353 
   Size:        731    1493    3423

  Fragments arranged by size: 

                3423    1493     731
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4234    4608    4754     387 
   Size:       1064     138    2645     374     146    1280

  Fragments arranged by size: 

                2645    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2712    2773    3710    4307    5094    5362    1239 
   Size:        393    1080      61     937     597     787     268    1524

  Fragments arranged by size: 

                1524    1080     937     787     597     393     268      61
            
 
EcoRI G'AATT_C

Cuts at:   2030    2956    3334    2030 
   Size:        926     378    4343

  Fragments arranged by size: 

                4343     926     378
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2914    2914 
   Size:       5647
 
FspI TGC'GCA

Cuts at:   4153    4153 
   Size:       5647
 
HaeII r_GCGC'y

Cuts at:   1678    4794    1678 
   Size:       3116    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    4288    4320    4362    4394    1373 
   Size:         32     135      32    2716      32      42      32    2626

  Fragments arranged by size: 

                2716    2626     135      42      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2049    2991    3237     669 
   Size:        988     281     111     942     246    3079

  Fragments arranged by size: 

                3079     988     942     281     246     111
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5647
 
HpaI GTT'AAC

Cuts at:   2049    3237    2049 
   Size:       1188    4459
 
KpnI G_GTAC'C

Cuts at:   2972    2972 
   Size:       5647
 
MfeI C'AATT_G

Cuts at:   3246    3246 
   Size:       5647
 
MluI A'CGCG_T

Cuts at:   5523    5523 
   Size:       5647
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4761    4945    1305 
   Size:       3456     184    2007

  Fragments arranged by size: 

                3456    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2432      10 
   Size:         54    1425     343     600    3225

  Fragments arranged by size: 

                3225    1425     600     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2021    2039    2171    2402    2672    2678    2846 
   Size:        648     855      18     132     231     270       6     168

Cuts at:   2846    3605    3964    4123     518 
   Size:        759     359     159    2042

  Fragments arranged by size: 

                2042     855     759     648     359     270     231     168
                 159     132      18       6
 
MspA1I CmG'CkG

Cuts at:   1606    2225    3063    3740    4888    5133    1606 
   Size:        619     838     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     838     677     619     245
 
NcoI C'CATG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    4156

  Fragments arranged by size: 

                4156     939     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5647
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5647
 
NotI GC'GGCC_GC

Cuts at:   3094    3094 
   Size:       5647
 
NsiI A_TGCA'T

Cuts at:   2467    2467 
   Size:       5647
 
NspI r_CATG'y

Cuts at:   1132    1229    3005    4550    1132 
   Size:         97    1776    1545    2229

  Fragments arranged by size: 

                2229    1776    1545      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5647
 
PciI A'CATG_T

Cuts at:   4546    4546 
   Size:       5647
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2800    1108 
   Size:       1692    3955
 
PmeI GTTT'AAAC

Cuts at:   2951    2951 
   Size:       5647
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3716    3748    5229    5261    3716 
   Size:         32    1481      32    4102

  Fragments arranged by size: 

                4102    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    2157    1613 
   Size:        544    5103
 
PsiI TTA'TAA

Cuts at:   3217    3217 
   Size:       5647
 
PstI C_TGCA'G

Cuts at:    830    2070    2329    2740    2999     830 
   Size:       1240     259     411     259    3478

  Fragments arranged by size: 

                3478    1240     411     259     259
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5647
 
PvuII CAG'CTG

Cuts at:   2225    2225 
   Size:       5647
 
SalI G'TCGA_C

Cuts at:   1936    2989    1936 
   Size:       1053    4594
 
SbfI CC_TGCA'GG

Cuts at:   2999    2999 
   Size:       5647
 
ScaI AGT'ACT

Cuts at:   3895    5607    3895 
   Size:       1712    3935
 
SfcI C'TryA_G

Cuts at:    826    1045    2066    2325    2503    2736    2866    2910    2931 
   Size:        219    1021     259     178     233     130      44      21

Cuts at:   2931    2995    4130    4811    5002     826 
   Size:         64    1135     681     191    1471

  Fragments arranged by size: 

                1471    1135    1021     681     259     233     219     191
                 178     130      64      44      21
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5647
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5647
 
SmaI CCC'GGG

Cuts at:   1872    2974    1872 
   Size:       1102    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3751    4652    4914     820 
   Size:        197     926    1808     901     262    1553

  Fragments arranged by size: 

                1808    1553     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3438     493 
   Size:       2945    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5647
 
SphI G_CATG'C

Cuts at:   1132    3005    1132 
   Size:       1873    3774
 
SspI AAT'ATT

Cuts at:      5      52    3571       5 
   Size:         47    3519    2081

  Fragments arranged by size: 

                3519    2081      47
 
StuI AGG'CCT

Cuts at:   1517    2708    2927    1517 
   Size:       1191     219    4237

  Fragments arranged by size: 

                4237    1191     219
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2235    3086     513 
   Size:        552     939     231     851    3074

  Fragments arranged by size: 

                3074     939     851     552     231
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3674    3691    3844    4029    4474     325 
   Size:       1255    2094      17     153     185     445    1498

  Fragments arranged by size: 

                2094    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2175    2772    3893 
   Size:        274      80      33      51    1443     198     597    1121

Cuts at:   3893    5605      96 
   Size:       1712     138

  Fragments arranged by size: 

                1712    1443    1121     597     274     198     138      80
                  51      33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5607    5639    5607 
   Size:         32    5615
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5647
 
XbaI T'CTAG_A

Cuts at:   2983    2983 
   Size:       5647
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2242    2797    2242 
   Size:        555    5092
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5647
 
XmaI C'CCGG_G

Cuts at:   1870    2972    1870 
   Size:       1102    4545
 
XmnI GAAnn'nnTTC

Cuts at:   2142    2486    3776    2142 
   Size:        344    1290    4013

  Fragments arranged by size: 

                4013    1290     344
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3455     276 
   Size:         53      83     186     936    1921    2468

  Fragments arranged by size: 

                2468    1921     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbsI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI
   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI
     EaeI     EagI     EarI     EciI  Eco57MI    EcoRI     FalI     FspI
    HaeII    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MluI
     MmeI     MscI     MslI   MspA1I     NcoI     NdeI     NheI     NotI
     NsiI     NspI     PasI     PciI    PflMI     PmeI     PpiI    PshAI
     PsiI     PstI     PvuI    PvuII     SalI     SbfI     ScaI     SfcI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SspI
     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     ApaI     AscI    AvrII     BaeI     BbeI     BplI
    BsiWI    BsmBI  BstZ17I   DraIII  EcoICRI    EcoNI EcoO109I    EcoRV
     FseI    FspAI     KasI     NaeI     NarI   NgoMIV     NruI     PacI
     PfoI     PmlI    PpuMI   PspOMI    PspXI     PsrI    RsrII     SacI
    SacII    SanDI     SapI    SexAI     SfiI     SfoI     SrfI     SwaI
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