(Circular) (Six-base) MAPSORT of: pFN21AE5962.seq Check: 2979 from: 1 to: 5641 pFN21AE5962 5641 bp With 182 enzymes: SgfI * December 8, 2014 13:48 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3451 278 Size: 53 83 186 936 1915 2468 Fragments arranged by size: 2468 1915 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2984 1937 Size: 1047 4594 Acc65I G'GTAC_C Cuts at: 2962 2962 Size: 5641 AclI AA'CG_TT Cuts at: 1563 3768 4141 1563 Size: 2205 373 3063 Fragments arranged by size: 3063 2205 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2191 2439 3704 5088 5356 1632 Size: 559 248 1265 1384 268 1917 Fragments arranged by size: 1917 1384 1265 559 268 248 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5444 AflIII A'CryG_T Cuts at: 1203 2723 2821 4540 5517 1203 Size: 1520 98 1719 977 1327 Fragments arranged by size: 1719 1520 1327 977 98 AgeI A'CCGG_T Cuts at: 4472 4472 Size: 5641 AhdI GACnn_n'nnGTC Cuts at: 4370 4370 Size: 5641 AleI CACnn'nnGTG Cuts at: 1166 2726 1166 Size: 1560 4081 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5641 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2592 2624 2592 Size: 32 5609 AlwNI CAG_nnn'CTG Cuts at: 3384 4956 3384 Size: 1572 4069 ApaI G_GGCC'C Cuts at: 2094 2094 Size: 5641 ApaLI G'TGCA_C Cuts at: 2786 3698 4854 2786 Size: 912 1156 3573 Fragments arranged by size: 3573 1156 912 ApoI r'AATT_y Cuts at: 1493 2950 3176 3328 5437 1493 Size: 1457 226 152 2109 1697 Fragments arranged by size: 2109 1697 1457 226 152 AseI AT'TA_AT Cuts at: 160 1032 4195 160 Size: 872 3163 1606 Fragments arranged by size: 3163 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5641 AvaI C'yCGr_G Cuts at: 1870 1943 2966 1870 Size: 73 1023 4545 Fragments arranged by size: 4545 1023 73 BamHI G'GATC_C Cuts at: 2971 3338 2971 Size: 367 5274 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2962 4417 618 Size: 325 217 627 1175 1455 1842 Fragments arranged by size: 1842 1455 1175 627 325 217 BanII G_rGCy'C Cuts at: 1391 2072 2094 2163 2178 2960 1391 Size: 681 22 69 15 782 4072 Fragments arranged by size: 4072 782 681 69 22 15 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4670 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2241 2275 3832 3866 1903 Size: 34 304 34 1557 34 3678 Fragments arranged by size: 3678 1557 304 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2063 2626 3533 4743 2063 Size: 563 907 1210 2961 Fragments arranged by size: 2961 1210 907 563 BclI T'GATC_A Cuts at: 1548 1548 Size: 5641 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4252 136 Size: 107 122 71 631 605 2580 1525 Fragments arranged by size: 2580 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5636 5636 Size: 5641 BlpI GC'TnA_GC Cuts at: 3058 3058 Size: 5641 Bme1580I G_kGCm'C Cuts at: 1790 2094 2790 3702 4858 1790 Size: 304 696 912 1156 2573 Fragments arranged by size: 2573 1156 912 696 304 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5641 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3372 4330 442 Size: 416 53 83 399 24 1955 958 1753 Fragments arranged by size: 1955 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5641 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2084 4301 1239 Size: 845 2217 2579 Fragments arranged by size: 2579 2217 845 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3766 4631 4929 3766 Size: 865 298 4478 Fragments arranged by size: 4478 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2782 4304 882 Size: 617 1283 1522 2219 Fragments arranged by size: 2219 1522 1283 617 BsaAI yAC'GTr Cuts at: 493 1204 3432 493 Size: 711 2228 2702 Fragments arranged by size: 2702 2228 711 BsaBI GATnn'nnATC Cuts at: 5635 5635 Size: 5641 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3448 3830 4465 275 Size: 53 83 186 936 1915 382 635 1451 Fragments arranged by size: 1915 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4074 4472 4746 4893 5304 4074 Size: 398 274 147 411 4411 Fragments arranged by size: 4411 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2309 2339 1178 Size: 30 165 30 906 30 4480 Fragments arranged by size: 4480 906 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5641 BseYI C'CCAG_C Cuts at: 2162 4844 5340 2162 Size: 2682 496 2463 Fragments arranged by size: 2682 2463 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2466 2528 1163 Size: 573 730 62 4276 Fragments arranged by size: 4276 730 573 62 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3091 3852 4880 1540 Size: 275 186 1090 761 1028 2301 Fragments arranged by size: 2301 1090 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2072 2412 2664 2790 2865 3702 3787 4858 2072 Size: 340 252 126 75 837 85 1071 2855 Fragments arranged by size: 2855 1071 837 340 252 126 85 75 BsmI GAATG_Cn' Cuts at: 2033 3152 3245 2033 Size: 1119 93 4429 Fragments arranged by size: 4429 1119 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2072 2094 2163 2178 2412 2664 2790 Size: 399 282 22 69 15 234 252 126 Cuts at: 2790 2865 2960 3702 3787 4858 1391 Size: 75 95 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 282 252 234 126 95 85 75 69 22 15 BspEI T'CCGG_A Cuts at: 5304 5304 Size: 5641 BspHI T'CATG_A Cuts at: 3530 5260 3530 Size: 1730 3911 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2996 844 Size: 2152 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3528 1394 Size: 429 1705 3507 Fragments arranged by size: 3507 1705 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2424 2439 4136 4310 65 Size: 1183 1176 15 1697 174 1396 Fragments arranged by size: 1697 1396 1183 1176 174 15 BsrFI r'CCGG_y Cuts at: 1700 1810 4285 4472 1700 Size: 110 2475 187 2869 Fragments arranged by size: 2869 2475 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5641 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5641 BssSI C'ACGA_G Cuts at: 3701 4713 3701 Size: 1012 4629 BstAPI GCAn_nnn'nTGC Cuts at: 794 2149 794 Size: 1355 4286 BstBI TT'CG_AA Cuts at: 4445 4445 Size: 5641 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5641 BstXI CCAn_nnnn'nTGG Cuts at: 2227 2227 Size: 5641 BstYI r'GATC_y Cuts at: 1972 2513 2971 3338 3723 3740 5192 5278 5290 Size: 541 458 367 385 17 1452 86 12 Cuts at: 5290 5636 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 541 458 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2226 513 Size: 552 939 222 3928 Fragments arranged by size: 3928 939 552 222 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4902 Fragments arranged by size: 4902 683 56 BtsI GCAGTG_nn' Cuts at: 2234 2685 3157 3951 3971 2234 Size: 451 472 794 20 3904 Fragments arranged by size: 3904 794 472 451 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5641 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5606 DraI TTT'AAA Cuts at: 2945 2945 Size: 5641 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4648 809 Size: 507 3332 1802 Fragments arranged by size: 3332 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2217 2229 2352 Size: 54 1425 50 275 18 387 12 123 Cuts at: 2352 2376 2682 3088 3977 5606 8 Size: 24 306 406 889 1629 43 Fragments arranged by size: 1629 1425 889 406 387 306 275 123 54 50 43 24 18 12 EagI C'GGCC_G Cuts at: 1537 1812 3088 1537 Size: 275 1276 4090 Fragments arranged by size: 4090 1276 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2484 3571 1353 Size: 1131 1087 3423 Fragments arranged by size: 3423 1131 1087 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2891 4228 4602 4748 387 Size: 1064 138 1302 1337 374 146 1280 Fragments arranged by size: 1337 1302 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2070 2070 Size: 5641 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2084 2191 2439 3704 4301 5088 5356 Size: 393 452 107 248 1265 597 787 268 Cuts at: 5356 1239 Size: 1524 Fragments arranged by size: 1524 1265 787 597 452 393 268 248 107 EcoO109I rG'GnC_Cy Cuts at: 2090 2091 2090 Size: 1 5640 EcoRI G'AATT_C Cuts at: 2950 3328 2950 Size: 378 5263 EcoRV GAT'ATC Cuts at: 2578 2578 Size: 5641 FspI TGC'GCA Cuts at: 4147 4147 Size: 5641 HaeII r_GCGC'y Cuts at: 1678 2886 4788 1678 Size: 1208 1902 2531 Fragments arranged by size: 2531 1902 1208 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4282 4314 4356 4388 1373 Size: 32 135 32 2710 32 42 32 2626 Fragments arranged by size: 2710 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2985 3231 669 Size: 988 281 1047 246 3079 Fragments arranged by size: 3079 1047 988 281 246 HindIII A'AGCT_T Cuts at: 748 2458 748 Size: 1710 3931 HpaI GTT'AAC Cuts at: 3231 3231 Size: 5641 KpnI G_GTAC'C Cuts at: 2966 2966 Size: 5641 MfeI C'AATT_G Cuts at: 3240 3240 Size: 5641 MluI A'CGCG_T Cuts at: 5517 5517 Size: 5641 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4755 4939 1305 Size: 3450 184 2007 Fragments arranged by size: 3450 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2219 2231 2354 2378 2684 Size: 54 1425 343 387 12 123 24 306 Cuts at: 2684 10 Size: 2967 Fragments arranged by size: 2967 1425 387 343 306 123 54 24 12 MslI CAynn'nnrTG Cuts at: 518 1166 2225 2726 3599 3958 4117 518 Size: 648 1059 501 873 359 159 2042 Fragments arranged by size: 2042 1059 873 648 501 359 159 MspA1I CmG'CkG Cuts at: 1606 2450 3057 3734 4882 5127 1606 Size: 844 607 677 1148 245 2120 Fragments arranged by size: 2120 1148 844 677 607 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2226 513 Size: 552 939 222 3928 Fragments arranged by size: 3928 939 552 222 NdeI CA'TA_TG Cuts at: 387 387 Size: 5641 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5641 NotI GC'GGCC_GC Cuts at: 3088 3088 Size: 5641 NspI r_CATG'y Cuts at: 1132 1229 2727 2807 2825 2923 2999 4544 1132 Size: 97 1498 80 18 98 76 1545 2229 Fragments arranged by size: 2229 1545 1498 98 97 80 76 18 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5641 PciI A'CATG_T Cuts at: 2723 2821 4540 2723 Size: 98 1719 3824 Fragments arranged by size: 3824 1719 98 PflMI CCAn_nnn'nTGG Cuts at: 1108 2771 1108 Size: 1663 3978 PmeI GTTT'AAAC Cuts at: 2945 2945 Size: 5641 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3710 3742 5223 5255 3710 Size: 32 1481 32 4096 Fragments arranged by size: 4096 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5641 PsiI TTA'TAA Cuts at: 3211 3211 Size: 5641 PspOMI G'GGCC_C Cuts at: 2090 2090 Size: 5641 PstI C_TGCA'G Cuts at: 830 2145 2513 2993 830 Size: 1315 368 480 3478 Fragments arranged by size: 3478 1315 480 368 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5641 PvuII CAG'CTG Cuts at: 2450 2450 Size: 5641 SacI G_AGCT'C Cuts at: 2072 2072 Size: 5641 SalI G'TCGA_C Cuts at: 1936 2983 1936 Size: 1047 4594 SbfI CC_TGCA'GG Cuts at: 2145 2993 2145 Size: 848 4793 ScaI AGT'ACT Cuts at: 3889 5601 3889 Size: 1712 3929 SfcI C'TryA_G Cuts at: 826 1045 2141 2509 2989 4124 4805 4996 826 Size: 219 1096 368 480 1135 681 191 1471 Fragments arranged by size: 1471 1135 1096 681 480 368 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5641 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5641 SmaI CCC'GGG Cuts at: 1872 2968 1872 Size: 1096 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3745 4646 4908 820 Size: 197 926 1802 901 262 1553 Fragments arranged by size: 1802 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3432 493 Size: 2939 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5641 SphI G_CATG'C Cuts at: 1132 2999 1132 Size: 1867 3774 SspI AAT'ATT Cuts at: 5 52 3565 5 Size: 47 3513 2081 Fragments arranged by size: 3513 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5641 StyI C'CwwG_G Cuts at: 513 1065 2004 2119 2226 3080 513 Size: 552 939 115 107 854 3074 Fragments arranged by size: 3074 939 854 552 115 107 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2562 3668 3685 3838 4023 4468 325 Size: 1255 982 1106 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1106 982 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2181 2245 2746 Size: 274 80 33 51 1443 204 64 501 Cuts at: 2746 3887 5599 96 Size: 1141 1712 138 Fragments arranged by size: 1712 1443 1141 501 274 204 138 80 64 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2118 2150 2309 2341 5601 5633 2118 Size: 32 159 32 3260 32 2126 Fragments arranged by size: 3260 2126 159 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5641 XbaI T'CTAG_A Cuts at: 2977 2977 Size: 5641 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2626 2626 Size: 5641 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5641 XmaI C'CCGG_G Cuts at: 1870 2966 1870 Size: 1096 4545 XmnI GAAnn'nnTTC Cuts at: 3770 3770 Size: 5641 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3449 276 Size: 53 83 186 936 1915 2468 Fragments arranged by size: 2468 1915 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI