(Linear) (Six-base) MAPSORT of: KIEE5962.seq Check: 6193 from: 1 to: 936 KIEE5962.orf With 182 enzymes: SgfI * December 5, 2014 13:16 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 186 434 936 Size: 186 248 502 Fragments arranged by size: 502 248 186 AflIII A'CryG_T Cuts at: 0 718 816 936 Size: 718 98 120 Fragments arranged by size: 718 120 98 AleI CACnn'nnGTG Cuts at: 0 721 936 Size: 721 215 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 587 619 936 Size: 587 32 317 Fragments arranged by size: 587 317 32 ApaI G_GGCC'C Cuts at: 0 89 936 Size: 89 847 ApaLI G'TGCA_C Cuts at: 0 781 936 Size: 781 155 BanII G_rGCy'C Cuts at: 0 67 89 158 173 936 Size: 67 22 69 15 763 Fragments arranged by size: 763 69 67 22 15 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 236 270 936 Size: 236 34 666 Fragments arranged by size: 666 236 34 BciVI GTATCCnnnnn_n' Cuts at: 0 58 621 936 Size: 58 563 315 Fragments arranged by size: 563 315 58 Bme1580I G_kGCm'C Cuts at: 0 89 785 936 Size: 89 696 151 Fragments arranged by size: 696 151 89 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 79 936 Size: 79 857 BsaI GGTCTCn'nnnn_ Cuts at: 0 777 936 Size: 777 159 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 304 334 936 Size: 304 30 602 Fragments arranged by size: 602 304 30 BseYI C'CCAG_C Cuts at: 0 157 936 Size: 157 779 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 461 523 936 Size: 461 62 413 Fragments arranged by size: 461 413 62 BsiHKAI G_wGCw'C Cuts at: 0 67 407 659 785 860 936 Size: 67 340 252 126 75 76 Fragments arranged by size: 340 252 126 76 75 67 BsmI GAATG_Cn' Cuts at: 0 28 936 Size: 28 908 Bsp1286I G_dGCh'C Cuts at: 0 67 89 158 173 407 659 785 860 Size: 67 22 69 15 234 252 126 75 Cuts at: 860 936 Size: 76 Fragments arranged by size: 252 234 126 76 75 69 67 22 15 BsrDI GCAATG_nn' Cuts at: 0 419 434 936 Size: 419 15 502 Fragments arranged by size: 502 419 15 BstAPI GCAn_nnn'nTGC Cuts at: 0 144 936 Size: 144 792 BstXI CCAn_nnnn'nTGG Cuts at: 0 222 936 Size: 222 714 BstYI r'GATC_y Cuts at: 0 508 936 Size: 508 428 BtgI C'CryG_G Cuts at: 0 221 936 Size: 221 715 BtsI GCAGTG_nn' Cuts at: 0 229 680 936 Size: 229 451 256 Fragments arranged by size: 451 256 229 EaeI y'GGCC_r Cuts at: 0 212 224 347 371 677 936 Size: 212 12 123 24 306 259 Fragments arranged by size: 306 259 212 123 24 12 EarI CTCTTCn'nnn_ Cuts at: 0 479 936 Size: 479 457 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 886 936 Size: 886 50 EcoICRI GAG'CTC Cuts at: 0 65 936 Size: 65 871 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 79 186 434 936 Size: 79 107 248 502 Fragments arranged by size: 502 248 107 79 EcoO109I rG'GnC_Cy Cuts at: 0 85 86 936 Size: 85 1 850 Fragments arranged by size: 850 85 1 EcoRV GAT'ATC Cuts at: 0 573 936 Size: 573 363 HaeII r_GCGC'y Cuts at: 0 881 936 Size: 881 55 HindIII A'AGCT_T Cuts at: 0 453 936 Size: 453 483 MscI TGG'CCA Cuts at: 0 214 226 349 373 679 936 Size: 214 12 123 24 306 257 Fragments arranged by size: 306 257 214 123 24 12 MslI CAynn'nnrTG Cuts at: 0 220 721 936 Size: 220 501 215 Fragments arranged by size: 501 220 215 MspA1I CmG'CkG Cuts at: 0 445 936 Size: 445 491 NcoI C'CATG_G Cuts at: 0 221 936 Size: 221 715 NspI r_CATG'y Cuts at: 0 722 802 820 918 936 Size: 722 80 18 98 18 Fragments arranged by size: 722 98 80 18 18 PciI A'CATG_T Cuts at: 0 718 816 936 Size: 718 98 120 Fragments arranged by size: 718 120 98 PflMI CCAn_nnn'nTGG Cuts at: 0 766 936 Size: 766 170 PspOMI G'GGCC_C Cuts at: 0 85 936 Size: 85 851 PstI C_TGCA'G Cuts at: 0 140 508 936 Size: 140 368 428 Fragments arranged by size: 428 368 140 PvuII CAG'CTG Cuts at: 0 445 936 Size: 445 491 SacI G_AGCT'C Cuts at: 0 67 936 Size: 67 869 SbfI CC_TGCA'GG Cuts at: 0 140 936 Size: 140 796 SfcI C'TryA_G Cuts at: 0 136 504 936 Size: 136 368 432 Fragments arranged by size: 432 368 136 StyI C'CwwG_G Cuts at: 0 114 221 936 Size: 114 107 715 Fragments arranged by size: 715 114 107 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 557 936 Size: 557 379 TatI w'GTAC_w Cuts at: 0 176 240 741 936 Size: 176 64 501 195 Fragments arranged by size: 501 195 176 64 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 113 145 304 336 936 Size: 113 32 159 32 600 Fragments arranged by size: 600 159 113 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 621 936 Size: 621 315 Enzymes that do cut: AcuI AflIII AleI AloI ApaI ApaLI BanII BcgI BciVI Bme1580I BpmI BsaI BsaXI BseYI BsgI BsiHKAI BsmI Bsp1286I BsrDI BstAPI BstXI BstYI BtgI BtsI EaeI EarI EciI EcoICRI Eco57MI EcoO109I EcoRV HaeII HindIII MscI MslI MspA1I NcoI NspI PciI PflMI PspOMI PstI PvuII SacI SbfI SfcI StyI TaqII TatI TstI XcmI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AlfI AlwNI ApoI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbsI BbvCI BclI BfrBI BglI BglII BlpI BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI BseRI BsiEI BsiWI BsmBI BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EagI EcoNI EcoRI FalI FseI FspI FspAI Hin4I HincII HpaI KasI KpnI MfeI MluI MmeI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI XhoI XmaI XmnI ZraI