################################## # NO CUT ENZYMES ( 167 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BamHI BbeI BbrPI BbsI BbvI BcgI BcnI BfaI BfrI BglI BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BsmFI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoRI EcoRV FauI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HindIII HpaII KasI KpnI KspI MaeI MaeII MamI MfeI MluI MroI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TaqI TseI Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AccI 841 GTCTAC GT^[AC][GT]AC AvaII 300 GGTCC G^G[AT]CC BalI 353 TGGCCA TGG^CCA BanI 16 GGCACC G^G[CT][GA]CC BanII 601 GGGCTC G[GA]GC[CT]^C BbvCI 132 CCTCAGC CCTCAGC(-5/-2) BciVI 62 GGATAC GTATCC(6/5) BclI 611 TGATCA T^GATCA BglII 552 AGATCT A^GATCT BpmI 40 CTCCAG CTGGAG(16/14) BsaI 7 GGTCTC GGTCTC(1/5) BsgI 743 GTGCAG GTGCAG(16/14) BspMI 724 ACCTGC ACCTGC(4/8) BsrDI 518 GCAATG GCAATG(2/0) BstEII 127 GGTAACC G^GT[ACGT]ACC BstPI 127 GGTAACC G^GT[ACGT]ACC BstXI 356 CCATGAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr13I 300 GGTCC G^G[ACGT]CC EaeI 353 TGGCCA [CT]^GGCC[GA] EarI 472 CTCTTC CTCTTC(1/4) EcoO65I 127 GGTAACC G^GT[ACGT]ACC EcoT14I 893 CCAAGG C^C[AT][AT]GG EcoT22I 659 ATGCAT ATGCA^T FbaI 611 TGATCA T^GATCA Fnu4HI 705 GCGGC GC^[ACGT]GC HincII 586 GTTAAC GT[CT]^[GA]AC HindII 586 GTTAAC GT[CT]^[GA]AC HpaI 586 GTTAAC GTT^AAC ItaI 705 GCGGC GC^[ACGT]GC Ksp632I 472 CTCTTC CTCTTC(1/4) MluNI 353 TGGCCA TGG^CCA MscI 353 TGGCCA TGG^CCA NsiI 659 ATGCAT ATGCA^T PvuII 43 CAGCTG CAG^CTG Sau96I 300 GGTCC G^G[ACGT]CC SfcI 879 CTACAG C^T[GA][CT]AG StuI 72 AGGCCT AGG^CCT StyI 893 CCAAGG C^C[AT][AT]GG Tsp509I 771 AATT ^AATT ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AfaI 183 GTAC GT^AC 853 GTAC AluI 44 AGCT AG^CT 361 AGCT AspHI 409 GTGCTC G[AT]GC[AT]^C 806 GTGCTC BsiHKAI 409 GTGCTC G[AT]GC[AT]^C 806 GTGCTC BsmI 53 GCATTC GAATGC(1/-1) 137 GCATTC BspHI 557 TCATGA T^CATGA 827 TCATGA BstYI 552 AGATCT [GA]^GATC[CT] 899 AGATCC DdeI 133 CTCAG C^T[ACGT]AG 670 CTGAG MflI 552 AGATCT [GA]^GATC[CT] 899 AGATCC MseI 287 TTAA T^TAA 587 TTAA MspA1I 43 CAGCTG C[AC]G^C[GT]G 703 CAGCGG NlaIV 16 GGCACC GG[ACGT]^[ACGT]CC 159 GGAGCC NspI 568 GCATGT [GA]CATG^[CT] 728 GCATGT PleI 634 GACTC GAGTC(4/5) 337 GAGTC RcaI 557 TCATGA T^CATGA 827 TCATGA RsaI 183 GTAC GT^AC 853 GTAC SmlI 709 CTCAAG C^T[CT][GA]AG 916 CTCAAG TfiI 786 GATTC G^A[AT]TC 912 GATTC Tru9I 287 TTAA T^TAA 587 TTAA Tsp45I 403 GTCAC ^GT[GC]AC 732 GTCAC XhoII 552 AGATCT [GA]^GATC[CT] 899 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 31 enzymes) # ######################################### AciI AlwI BmyI BsaJI BsiYI BslI BsmAI Bsp1286I BssKI BstF5I BstNI Cac8I DpnII EcoRII FokI HaeIII HinfI HphI MaeIII MboI MboII MnlI MslI MvaI MwoI NdeII NlaIII Sau3AI ScrFI SfaNI TspRI