(Circular) (Six-base) MAPSORT of: pFN21AE6012.seq Check: 8869 from: 1 to: 5647 pFN21AE6012 5647 bp With 182 enzymes: SgfI * December 8, 2014 13:49 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2738 2738 Size: 5647 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3457 278 Size: 53 83 186 936 1921 2468 Fragments arranged by size: 2468 1921 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2847 2990 1937 Size: 910 143 4594 Fragments arranged by size: 4594 910 143 Acc65I G'GTAC_C Cuts at: 2968 2968 Size: 5647 AclI AA'CG_TT Cuts at: 1563 3774 4147 1563 Size: 2211 373 3063 Fragments arranged by size: 3063 2211 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3710 5094 5362 1632 Size: 2078 1384 268 1917 Fragments arranged by size: 2078 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5450 AflIII A'CryG_T Cuts at: 1203 4546 5523 1203 Size: 3343 977 1327 Fragments arranged by size: 3343 1327 977 AgeI A'CCGG_T Cuts at: 4478 4478 Size: 5647 AhdI GACnn_n'nnGTC Cuts at: 4376 4376 Size: 5647 AleI CACnn'nnGTG Cuts at: 1166 2153 2745 1166 Size: 987 592 4068 Fragments arranged by size: 4068 987 592 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2546 1184 Size: 1362 4285 AlwNI CAG_nnn'CTG Cuts at: 3390 4962 3390 Size: 1572 4075 ApaLI G'TGCA_C Cuts at: 3704 4860 3704 Size: 1156 4491 ApoI r'AATT_y Cuts at: 1493 2956 3182 3334 5443 1493 Size: 1463 226 152 2109 1697 Fragments arranged by size: 2109 1697 1463 226 152 AseI AT'TA_AT Cuts at: 160 1032 4201 160 Size: 872 3169 1606 Fragments arranged by size: 3169 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5647 AvaI C'yCGr_G Cuts at: 1870 1943 2972 1870 Size: 73 1029 4545 Fragments arranged by size: 4545 1029 73 BamHI G'GATC_C Cuts at: 2977 3344 2977 Size: 367 5280 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2021 2968 4423 618 Size: 325 217 627 234 947 1455 1842 Fragments arranged by size: 1842 1455 947 627 325 234 217 BanII G_rGCy'C Cuts at: 1391 2610 2966 1391 Size: 1219 356 4072 Fragments arranged by size: 4072 1219 356 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4676 BbvCI CC'TCA_GC Cuts at: 2138 2138 Size: 5647 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3838 3872 1903 Size: 34 50 34 1817 34 3678 Fragments arranged by size: 3678 1817 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2061 3539 4749 2061 Size: 1478 1210 2959 Fragments arranged by size: 2959 1478 1210 BclI T'GATC_A Cuts at: 1548 2616 1548 Size: 1068 4579 BfrBI ATG'CAT Cuts at: 2666 2666 Size: 5647 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4258 136 Size: 107 122 71 631 605 2586 1525 Fragments arranged by size: 2586 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2557 5642 2557 Size: 3085 2562 BlpI GC'TnA_GC Cuts at: 3064 3064 Size: 5647 Bme1580I G_kGCm'C Cuts at: 1790 2772 3708 4864 1790 Size: 982 936 1156 2573 Fragments arranged by size: 2573 1156 982 936 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5647 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3378 4336 442 Size: 416 53 83 399 24 1961 958 1753 Fragments arranged by size: 1961 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5647 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2030 4307 1239 Size: 791 2277 2579 Fragments arranged by size: 2579 2277 791 Bpu10I CC'TnA_GC Cuts at: 2138 2138 Size: 5647 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2699 2906 3772 4637 4935 2699 Size: 207 866 865 298 3411 Fragments arranged by size: 3411 866 865 298 207 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2018 4310 882 Size: 617 519 2292 2219 Fragments arranged by size: 2292 2219 617 519 BsaAI yAC'GTr Cuts at: 493 1204 3438 493 Size: 711 2234 2702 Fragments arranged by size: 2702 2234 711 BsaBI GATnn'nnATC Cuts at: 5641 5641 Size: 5647 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3454 3836 4471 275 Size: 53 83 186 936 1921 382 635 1451 Fragments arranged by size: 1921 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4080 4478 4752 4899 5310 4080 Size: 398 274 147 411 4417 Fragments arranged by size: 4417 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2105 2135 2315 2345 1178 Size: 30 165 30 702 30 180 30 4480 Fragments arranged by size: 4480 702 180 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5647 BseYI C'CCAG_C Cuts at: 2008 4850 5346 2008 Size: 2842 496 2309 Fragments arranged by size: 2842 2309 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2769 1163 Size: 573 1033 4041 Fragments arranged by size: 4041 1033 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3097 3858 4886 1540 Size: 275 186 1096 761 1028 2301 Fragments arranged by size: 2301 1096 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2418 2815 3708 3793 4864 2418 Size: 397 893 85 1071 3201 Fragments arranged by size: 3201 1071 893 397 85 BsmI GAATG_Cn' Cuts at: 2058 2142 3158 3251 2058 Size: 84 1016 93 4454 Fragments arranged by size: 4454 1016 93 84 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2418 2610 2772 2815 2966 3708 3793 Size: 399 628 192 162 43 151 742 85 Cuts at: 3793 4864 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 628 399 192 162 151 85 43 BspEI T'CCGG_A Cuts at: 5310 5310 Size: 5647 BspHI T'CATG_A Cuts at: 2562 2832 3536 5266 2562 Size: 270 704 1730 2943 Fragments arranged by size: 2943 1730 704 270 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2738 3002 844 Size: 1894 264 3489 Fragments arranged by size: 3489 1894 264 BsrBI CCG'CTC Cuts at: 1394 1823 3534 1394 Size: 429 1711 3507 Fragments arranged by size: 3507 1711 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2530 4142 4316 65 Size: 1183 1282 1612 174 1396 Fragments arranged by size: 1612 1396 1282 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4291 4478 1700 Size: 110 2481 187 2869 Fragments arranged by size: 2869 2481 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5647 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5647 BssSI C'ACGA_G Cuts at: 3707 4719 3707 Size: 1012 4635 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5647 BstBI TT'CG_AA Cuts at: 4451 4451 Size: 5647 BstEII G'GTnAC_C Cuts at: 1184 2132 1184 Size: 948 4699 BstXI CCAn_nnnn'nTGG Cuts at: 2368 2368 Size: 5647 BstYI r'GATC_y Cuts at: 1972 2557 2904 2977 3344 3729 3746 5198 5284 Size: 585 347 73 367 385 17 1452 86 Cuts at: 5284 5296 5642 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 585 385 367 347 346 86 73 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5095 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4908 Fragments arranged by size: 4908 683 56 BtsI GCAGTG_nn' Cuts at: 2757 3163 3957 3977 2757 Size: 406 794 20 4427 Fragments arranged by size: 4427 794 406 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5647 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5612 DraI TTT'AAA Cuts at: 2951 2951 Size: 5647 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4654 809 Size: 507 3338 1802 Fragments arranged by size: 3338 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2358 3094 3983 Size: 54 1425 50 275 18 528 736 889 Cuts at: 3983 5612 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 736 528 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3094 1537 Size: 275 1282 4090 Fragments arranged by size: 4090 1282 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2483 3577 1353 Size: 1130 1094 3423 Fragments arranged by size: 3423 1130 1094 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4234 4608 4754 387 Size: 1064 138 2645 374 146 1280 Fragments arranged by size: 2645 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2030 3710 4307 5094 5362 1239 Size: 393 398 1680 597 787 268 1524 Fragments arranged by size: 1680 1524 787 597 398 393 268 EcoRI G'AATT_C Cuts at: 2956 3334 2956 Size: 378 5269 FspI TGC'GCA Cuts at: 4153 4153 Size: 5647 HaeII r_GCGC'y Cuts at: 1678 4794 1678 Size: 3116 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2356 2388 4288 4320 4362 Size: 32 135 32 784 32 1900 32 42 Cuts at: 4362 4394 1373 Size: 32 2626 Fragments arranged by size: 2626 1900 784 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2593 2991 3237 669 Size: 988 281 655 398 246 3079 Fragments arranged by size: 3079 988 655 398 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5647 HpaI GTT'AAC Cuts at: 2593 3237 2593 Size: 644 5003 KpnI G_GTAC'C Cuts at: 2972 2972 Size: 5647 MfeI C'AATT_G Cuts at: 3246 3246 Size: 5647 MluI A'CGCG_T Cuts at: 5523 5523 Size: 5647 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4761 4945 1305 Size: 3456 184 2007 Fragments arranged by size: 3456 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2360 10 Size: 54 1425 343 528 3297 Fragments arranged by size: 3297 1425 528 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2153 2732 2745 3605 3964 4123 518 Size: 648 987 579 13 860 359 159 2042 Fragments arranged by size: 2042 987 860 648 579 359 159 13 MspA1I CmG'CkG Cuts at: 1606 2050 2710 3063 3740 4888 5133 1606 Size: 444 660 353 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 660 444 353 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5095 NdeI CA'TA_TG Cuts at: 387 387 Size: 5647 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5647 NotI GC'GGCC_GC Cuts at: 3094 3094 Size: 5647 NsiI A_TGCA'T Cuts at: 2668 2668 Size: 5647 NspI r_CATG'y Cuts at: 1132 1229 2577 2737 3005 4550 1132 Size: 97 1348 160 268 1545 2229 Fragments arranged by size: 2229 1545 1348 268 160 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5647 PciI A'CATG_T Cuts at: 4546 4546 Size: 5647 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5647 PmeI GTTT'AAAC Cuts at: 2951 2951 Size: 5647 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2697 2729 3716 3748 5229 5261 2697 Size: 32 987 32 1481 32 3083 Fragments arranged by size: 3083 1481 987 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5647 PsiI TTA'TAA Cuts at: 3217 3217 Size: 5647 PstI C_TGCA'G Cuts at: 830 2999 830 Size: 2169 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5647 PvuII CAG'CTG Cuts at: 2050 2050 Size: 5647 SalI G'TCGA_C Cuts at: 1936 2989 1936 Size: 1053 4594 SbfI CC_TGCA'GG Cuts at: 2999 2999 Size: 5647 ScaI AGT'ACT Cuts at: 3895 5607 3895 Size: 1712 3935 SfcI C'TryA_G Cuts at: 826 1045 2884 2995 4130 4811 5002 826 Size: 219 1839 111 1135 681 191 1471 Fragments arranged by size: 1839 1471 1135 681 219 191 111 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5647 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5647 SmaI CCC'GGG Cuts at: 1872 2974 1872 Size: 1102 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2714 2921 3751 4652 4914 820 Size: 197 926 771 207 830 901 262 1553 Fragments arranged by size: 1553 926 901 830 771 262 207 197 SnaBI TAC'GTA Cuts at: 493 3438 493 Size: 2945 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5647 SphI G_CATG'C Cuts at: 1132 3005 1132 Size: 1873 3774 SspI AAT'ATT Cuts at: 5 52 3571 5 Size: 47 3519 2081 Fragments arranged by size: 3519 2081 47 StuI AGG'CCT Cuts at: 1517 2079 1517 Size: 562 5085 StyI C'CwwG_G Cuts at: 513 1065 2898 3086 513 Size: 552 1833 188 3074 Fragments arranged by size: 3074 1833 552 188 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2878 3674 3691 3844 4029 4474 325 Size: 1255 1298 796 17 153 185 445 1498 Fragments arranged by size: 1498 1298 1255 796 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2187 3893 5605 Size: 274 80 33 51 1443 210 1706 1712 Cuts at: 5605 96 Size: 138 Fragments arranged by size: 1712 1706 1443 274 210 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2315 2347 5607 5639 2315 Size: 32 3260 32 2323 Fragments arranged by size: 3260 2323 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5647 XbaI T'CTAG_A Cuts at: 2983 2983 Size: 5647 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5647 XmaI C'CCGG_G Cuts at: 1870 2972 1870 Size: 1102 4545 XmnI GAAnn'nnTTC Cuts at: 3776 3776 Size: 5647 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3455 276 Size: 53 83 186 936 1921 2468 Fragments arranged by size: 2468 1921 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI