################################## # NO CUT ENZYMES ( 164 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AfaI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BamHI BanI BbeI BbvI BcgI BciVI BclI BfrI BglI BglII BlnI BpmI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1407I BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I Cfr10I CpoI DraI DraII DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI Fnu4HI FseI FspI HgaI Hin1I HincII HindII HindIII HpaI ItaI KasI KpnI Ksp632I KspI MamI MfeI MluI MroI MspA1I MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI TseI Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AvaII 887 GGACC G^G[AT]CC BalI 194 TGGCCA TGG^CCA BanII 175 GAGCCC G[GA]GC[CT]^C BbrPI 720 CACGTG CAC^GTG BbvCI 340 GCTGAGG CCTCAGC(-5/-2) BfaI 145 CTAG C^TAG BlpI 700 GCTCAGC GC^T[ACGT]AGC Bpu1102I 700 GCTCAGC GC^T[ACGT]AGC BsaAI 720 CACGTG [CT]AC^GT[GA] BsmFI 458 GTCCC GGGAC(10/14) BspDI 788 ATCGAT AT^CGAT BspLU11I 77 ACATGT A^CATGT BssSI 274 CACGAG CACGAG(-5/-1) BstF5I 416 CATCC GGATG(2/0) BstYI 83 GGATCT [GA]^GATC[CT] Cac8I 245 GCATGC GC[ACGT]^[ACGT]GC CelII 700 GCTCAGC GC^T[ACGT]AGC CfoI 728 GCGC GCG^C ClaI 788 ATCGAT AT^CGAT DsaI 479 CCATGG C^C[GA][CT]GG EcoT14I 479 CCATGG C^C[AT][AT]GG FokI 416 CATCC GGATG(9/13) HaeII 727 GGCGCT [GA]GCGC^[CT] HhaI 728 GCGC GCG^C HinP1I 728 GCGC G^CGC MaeI 145 CTAG C^TAG MaeII 721 ACGT A^CGT MflI 83 GGATCT [GA]^GATC[CT] MluNI 194 TGGCCA TGG^CCA MscI 194 TGGCCA TGG^CCA MseI 281 TTAA T^TAA NcoI 479 CCATGG C^CATGG NlaIV 174 GGAGCC GG[ACGT]^[ACGT]CC PflMI 420 CCAAGTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 720 CACGTG CAC^GTG PmlI 720 CACGTG CAC^GTG SphI 245 GCATGC GCATG^C StyI 479 CCATGG C^C[AT][AT]GG Tru9I 281 TTAA T^TAA Tsp45I 34 GTCAC ^GT[GC]AC TspRI 327 CACTG CA[GC]TG[ACGT][ACGT]^ Van91I 420 CCAAGTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 83 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AciI 498 CCGC CCGC(-3/-1) 681 CCGC AflIII 77 ACATGT A^C[GA][CT]GT 721 ACGTGT AluI 291 AGCT AG^CT 704 AGCT AlwI 315 GATCC GGATC(4/5) 83 GGATC BcnI 89 CCGGG CC^[GC]GG 171 CCGGG BsaJI 57 CCCAGG C^C[ACGT][ACGT]GG 479 CCATGG BsrDI 555 CATTGC GCAATG(2/0) 128 GCAATG DpnII 84 GATC ^GATC 315 GATC EaeI 194 TGGCCA [CT]^GGCC[GA] 377 TGGCCG HapII 89 CCGG C^CGG 171 CCGG HpaII 89 CCGG C^CGG 171 CCGG HphI 882 GGTGA GGTGA(8/7) 27 TCACC MaeIII 34 GTCAC ^GT[ACGT]AC 812 GTTAC MboI 84 GATC ^GATC 315 GATC MslI 327 CACTGGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 435 CATTAGCATG MspI 89 CCGG C^CGG 171 CCGG NciI 89 CCGGG CC^[GC]GG 171 CCGGG NdeII 84 GATC ^GATC 315 GATC PleI 165 GACTC GAGTC(4/5) 740 GAGTC Sau3AI 84 GATC ^GATC 315 GATC SexAI 632 ACCTGGT A^CC[AT]GGT 807 ACCAGGT TaqI 789 TCGA T^CGA 900 TCGA TfiI 53 GAATC G^A[AT]TC 897 GATTC Tsp509I 453 AATT ^AATT 563 AATT TthHB8I 789 TCGA T^CGA 900 TCGA XcmI 206 CCACTATTGATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 318 CCATGCCTTCACTGG ######################################### # 3 OR MORE CUT ENZYMES ( 25 enzymes) # ######################################### AspHI BbsI BmyI BpuAI BsiHKAI BsiYI BslI Bsp1286I BsrI BssKI BstNI Cfr13I DdeI EcoRII HaeIII HinfI MboII MnlI MvaI MwoI NlaIII NspI Sau96I ScrFI SfaNI