################################## # NO CUT ENZYMES ( 168 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI Asp700I Asp718I AspEI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRV EcoT14I EcoT22I FauI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HindIII HpaI KasI KpnI Ksp632I KspI MaeII MaeIII MluI MluNI MroI MscI MspA1I MvnI NaeI NarI NcoI NgoMIV NheI NlaIV NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI Psp1406I PvuI PvuII RsrII SacI SacII SalI SapI Sau96I SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI StuI StyI SwaI TaqI Tsp45I Tth111I TthHB8I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AccI 628 GTAGAC GT^[AC][GT]AC AciI 789 CCGC CCGC(-3/-1) AflII 706 CTTAAG C^TTAAG AluI 704 AGCT AG^CT AseI 817 ATTAAT AT^TAAT AsnI 817 ATTAAT AT^TAAT AspHI 611 GAGCAC G[AT]GC[AT]^C BcnI 797 CCCGG CC^[GC]GG BfrI 706 CTTAAG C^TTAAG BpmI 334 CTGGAG CTGGAG(16/14) BsaBI 84 GATCTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsgI 740 GTGCAG GTGCAG(16/14) BsiHKAI 611 GAGCAC G[AT]GC[AT]^C BsiYI 431 CCCAAATAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 431 CCCAAATAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BspHI 557 TCATGA T^CATGA BspMI 758 ACCTGC ACCTGC(4/8) BsrDI 642 CATTGC GCAATG(2/0) BstYI 83 GGATCT [GA]^GATC[CT] EaeI 776 TGGCCG [CT]^GGCC[GA] EcoRI 307 GAATTC G^AATTC HapII 798 CCGG C^CGG HincII 926 GTCAAC GT[CT]^[GA]AC HindII 926 GTCAAC GT[CT]^[GA]AC HpaII 798 CCGG C^CGG HphI 32 TCACC GGTGA(8/7) MamI 84 GATCTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 869 CAATTG C^AATTG MflI 83 GGATCT [GA]^GATC[CT] MspI 798 CCGG C^CGG MunI 869 CAATTG C^AATTG NciI 797 CCCGG CC^[GC]GG NdeI 20 CATATG CA^TATG PleI 337 GAGTC GAGTC(4/5) PshAI 414 GACAACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 817 ATTAAT AT^TAAT PstI 99 CTGCAG CTGCA^G RcaI 557 TCATGA T^CATGA ScaI 330 AGTACT AGT^ACT SfcI 99 CTGCAG C^T[GA][CT]AG SphI 461 GCATGC GCATG^C XhoII 83 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AcsI 278 AAATTT [GA]^AATT[CT] 307 GAATTC AlwI 663 GATCC GGATC(4/5) 83 GGATC ApoI 278 AAATTT [GA]^AATT[CT] 307 GAATTC BanII 160 GAGCCC G[GA]GC[CT]^C 175 GAGCCC BbvI 101 GCAGC GCAGC(8/12) 702 GCAGC BclI 353 TGATCA T^GATCA 746 TGATCA BsaJI 58 CCAGGG C^C[ACGT][ACGT]GG 302 CCCAGG BsmAI 542 GTCTC GTCTC(1/5) 805 GTCTC BsrI 333 ACTGG ACTGG(1/-1) 678 ACTGG Cac8I 292 GCCTGC GC[ACGT]^[ACGT]GC 461 GCATGC FbaI 353 TGATCA T^GATCA 746 TGATCA Fnu4HI 101 GCAGC GC^[ACGT]GC 702 GCAGC HaeIII 567 GGCC GG^CC 777 GGCC ItaI 101 GCAGC GC^[ACGT]GC 702 GCAGC MwoI 162 GCCCTCCTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 292 GCCTGCTTTGC TfiI 323 GATTC G^A[AT]TC 371 GATTC TseI 101 GCAGC G^C[AT]GC 702 GCAGC XcmI 35 CCACCTTCTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 764 CCATCATCAACCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 30 enzymes) # ######################################### AfaI BfaI BmyI Bsp1286I BssKI BstF5I BstNI DdeI DpnII EcoRII FokI HinfI MaeI MboI MboII MnlI MseI MslI MvaI NdeII NlaIII NspI RsaI Sau3AI ScrFI SfaNI SmlI Tru9I Tsp509I TspRI