Restriction map for pFN21AE5987
 (Circular) (Six-base) MAPSORT of: pFN21AE5987.seq  Check: 9445  from: 1  to: 5644



pFN21AE5987  5644 bp

 With 182 enzymes: SgfI * 

                        December 8, 2014 13:49  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3454     278 
   Size:         53      83     186     936    1918    2468

  Fragments arranged by size: 

                2468    1918     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2634    2987    1937 
   Size:        697     353    4594

  Fragments arranged by size: 

                4594     697     353
 
Acc65I G'GTAC_C

Cuts at:   2965    2965 
   Size:       5644
 
AclI AA'CG_TT

Cuts at:   1563    3771    4144    1563 
   Size:       2208     373    3063

  Fragments arranged by size: 

                3063    2208     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    3707    5091    5359    1632 
   Size:       2075    1384     268    1917

  Fragments arranged by size: 

                2075    1917    1384     268
 
AflII C'TTAA_G

Cuts at:    820    1017    2711     820 
   Size:        197    1694    3753

  Fragments arranged by size: 

                3753    1694     197
 
AflIII A'CryG_T

Cuts at:   1203    4543    5520    1203 
   Size:       3340     977    1327

  Fragments arranged by size: 

                3340    1327     977
 
AgeI A'CCGG_T

Cuts at:   4475    4475 
   Size:       5644
 
AhdI GACnn_n'nnGTC

Cuts at:   4373    4373 
   Size:       5644
 
AleI CACnn'nnGTG

Cuts at:   1166    2742    1166 
   Size:       1576    4068
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5644
 
AlwNI CAG_nnn'CTG

Cuts at:   3387    4959    3387 
   Size:       1572    4072
 
ApaLI G'TGCA_C

Cuts at:   3701    4857    3701 
   Size:       1156    4488
 
ApoI r'AATT_y

Cuts at:   1493    2283    2312    2953    3179    3331    5440    1493 
   Size:        790      29     641     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     790     641     226     152      29
 
AseI AT'TA_AT

Cuts at:    160    1032    2823    4198     160 
   Size:        872    1791    1375    1606

  Fragments arranged by size: 

                1791    1606    1375     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5644
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2969    1870 
   Size:         73    1026    4545

  Fragments arranged by size: 

                4545    1026      73
 
BamHI G'GATC_C

Cuts at:   2974    3341    2974 
   Size:        367    5277
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2965    4420     618 
   Size:        325     217     627    1178    1455    1842

  Fragments arranged by size: 

                1842    1455    1178     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2169    2184    2963    1391 
   Size:        778      15     779    4072

  Fragments arranged by size: 

                4072     779     778      15
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    4673
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3835    3869    1903 
   Size:         34    1898      34    3678

  Fragments arranged by size: 

                3678    1898      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3536    4746    3536 
   Size:       1210    4434
 
BclI T'GATC_A

Cuts at:   1548    2358    2751    1548 
   Size:        810     393    4441

  Fragments arranged by size: 

                4441     810     393
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4255     136 
   Size:        107     122      71     631     605    2583    1525

  Fragments arranged by size: 

                2583    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   5639    5639 
   Size:       5644
 
BlpI GC'TnA_GC

Cuts at:   3061    3061 
   Size:       5644
 
Bme1580I G_kGCm'C

Cuts at:   1790    3705    4861    1790 
   Size:       1915    1156    2573

  Fragments arranged by size: 

                2573    1915    1156
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5644
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2693    3375    4333 
   Size:        416      53      83     399      24    1276     682     958

Cuts at:   4333     442 
   Size:       1753

  Fragments arranged by size: 

                1753    1276     958     682     416     399      83      53
                  24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5644
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2360    4304    1239 
   Size:       1121    1944    2579

  Fragments arranged by size: 

                2579    1944    1121
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2527    2657    3769    4634    4932    2527 
   Size:        130    1112     865     298    3239

  Fragments arranged by size: 

                3239    1112     865     298     130
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4307     882 
   Size:        617    2808    2219

  Fragments arranged by size: 

                2808    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3435     493 
   Size:        711    2231    2702

  Fragments arranged by size: 

                2702    2231     711
 
BsaBI GATnn'nnATC

Cuts at:   2093    5638    2093 
   Size:       3545    2099
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3451    3833    4468     275 
   Size:         53      83     186     936    1918     382     635    1451

  Fragments arranged by size: 

                1918    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   4077    4475    4749    4896    5307    4077 
   Size:        398     274     147     411    4414

  Fragments arranged by size: 

                4414     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    5419

  Fragments arranged by size: 

                5419     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    1186 
   Size:       5644
 
BseYI C'CCAG_C

Cuts at:   4847    5343    4847 
   Size:        496    5148
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2766    1163 
   Size:        573    1030    4041

  Fragments arranged by size: 

                4041    1030     573
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3094    3855    4883    1540 
   Size:        275     186    1093     761    1028    2301

  Fragments arranged by size: 

                2301    1093    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2620    3705    3790    4861    2620 
   Size:       1085      85    1071    3403

  Fragments arranged by size: 

                3403    1085    1071      85
 
BsmI GAATG_Cn'

Cuts at:   3155    3248    3155 
   Size:         93    5551
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2169    2184    2620    2963    3705    3790    4861 
   Size:        399     379      15     436     343     742      85    1071

Cuts at:   4861    1391 
   Size:       2174

  Fragments arranged by size: 

                2174    1071     742     436     399     379     343      85
                  15
 
BspEI T'CCGG_A

Cuts at:   5307    5307 
   Size:       5644
 
BspHI T'CATG_A

Cuts at:   2562    3533    5263    2562 
   Size:        971    1730    2943

  Fragments arranged by size: 

                2943    1730     971
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2772    2999     844 
   Size:       1928     227    3489

  Fragments arranged by size: 

                3489    1928     227
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3531    1394 
   Size:        429    1708    3507

  Fragments arranged by size: 

                3507    1708     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2646    4139    4313      65 
   Size:       1183    1398    1493     174    1396

  Fragments arranged by size: 

                1493    1398    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4288    4475    1700 
   Size:        110    2478     187    2869

  Fragments arranged by size: 

                2869    2478     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5644
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5644
 
BssSI C'ACGA_G

Cuts at:   3704    4716    3704 
   Size:       1012    4632
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5644
 
BstBI TT'CG_AA

Cuts at:   4448    4448 
   Size:       5644
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5644
 
BstYI r'GATC_y

Cuts at:   1972    2088    2974    3341    3726    3743    5195    5281    5293 
   Size:        116     886     367     385      17    1452      86      12

Cuts at:   5293    5639    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1452     886     385     367     346     116      86
                  17      12
 
BtgI C'CryG_G

Cuts at:    513    1065     513 
   Size:        552    5092
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4905

  Fragments arranged by size: 

                4905     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2754    3160    3954    3974    2754 
   Size:        406     794      20    4424

  Fragments arranged by size: 

                4424     794     406      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5644
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5609
 
DraI TTT'AAA

Cuts at:   2948    2948 
   Size:       5644
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4651     809 
   Size:        507    3335    1802

  Fragments arranged by size: 

                3335    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2781    3091    3980 
   Size:         54    1425      50     275      18     951     310     889

Cuts at:   3980    5609       8 
   Size:       1629      43

  Fragments arranged by size: 

                1629    1425     951     889     310     275      54      50
                  43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3091    1537 
   Size:        275    1279    4090

  Fragments arranged by size: 

                4090    1279     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    3574    1353 
   Size:       2221    3423
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4231    4605    4751     387 
   Size:       1064     138    2642     374     146    1280

  Fragments arranged by size: 

                2642    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2360    3707    4304    5091    5359    1239 
   Size:        393     728    1347     597     787     268    1524

  Fragments arranged by size: 

                1524    1347     787     728     597     393     268
 
EcoRI G'AATT_C

Cuts at:   2312    2953    3331    2312 
   Size:        641     378    4625

  Fragments arranged by size: 

                4625     641     378
 
FspI TGC'GCA

Cuts at:   4150    4150 
   Size:       5644
 
HaeII r_GCGC'y

Cuts at:   1678    4791    1678 
   Size:       3113    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    4285    4317    4359    4391    1373 
   Size:         32     135      32    2713      32      42      32    2626

  Fragments arranged by size: 

                2713    2626     135      42      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2933    2988    3234     669 
   Size:        988     281     995      55     246    3079

  Fragments arranged by size: 

                3079     995     988     281     246      55
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5644
 
HpaI GTT'AAC

Cuts at:   3234    3234 
   Size:       5644
 
KpnI G_GTAC'C

Cuts at:   2969    2969 
   Size:       5644
 
MfeI C'AATT_G

Cuts at:   2874    3243    2874 
   Size:        369    5275
 
MluI A'CGCG_T

Cuts at:   5520    5520 
   Size:       5644
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4758    4942    1305 
   Size:       3453     184    2007

  Fragments arranged by size: 

                3453    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3822

  Fragments arranged by size: 

                3822    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2556    2651    2742    3602    3961    4120     518 
   Size:        648    1390      95      91     860     359     159    2042

  Fragments arranged by size: 

                2042    1390     860     648     359     159      95      91
            
 
MspA1I CmG'CkG

Cuts at:   1606    3060    3737    4885    5130    1606 
   Size:       1454     677    1148     245    2120

  Fragments arranged by size: 

                2120    1454    1148     677     245
 
NcoI C'CATG_G

Cuts at:    513    1065     513 
   Size:        552    5092
 
NdeI CA'TA_TG

Cuts at:    387    2026     387 
   Size:       1639    4005
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5644
 
NotI GC'GGCC_GC

Cuts at:   3091    3091 
   Size:       5644
 
NspI r_CATG'y

Cuts at:   1132    1229    2113    2470    2810    3002    4547    1132 
   Size:         97     884     357     340     192    1545    2229

  Fragments arranged by size: 

                2229    1545     884     357     340     192      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5644
 
PciI A'CATG_T

Cuts at:   4543    4543 
   Size:       5644
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5644
 
PmeI GTTT'AAAC

Cuts at:   2948    2948 
   Size:       5644
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3713    3745    5226    5258    3713 
   Size:         32    1481      32    4099

  Fragments arranged by size: 

                4099    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    2423    1613 
   Size:        810    4834
 
PsiI TTA'TAA

Cuts at:   3214    3214 
   Size:       5644
 
PstI C_TGCA'G

Cuts at:    830    2108    2996     830 
   Size:       1278     888    3478

  Fragments arranged by size: 

                3478    1278     888
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5644
 
SalI G'TCGA_C

Cuts at:   1936    2986    1936 
   Size:       1050    4594
 
SbfI CC_TGCA'GG

Cuts at:   2996    2996 
   Size:       5644
 
ScaI AGT'ACT

Cuts at:   2337    3892    5604    2337 
   Size:       1555    1712    2377

  Fragments arranged by size: 

                2377    1712    1555
 
SfcI C'TryA_G

Cuts at:    826    1045    2104    2992    4127    4808    4999     826 
   Size:        219    1059     888    1135     681     191    1471

  Fragments arranged by size: 

                1471    1135    1059     888     681     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5644
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5644
 
SmaI CCC'GGG

Cuts at:   1872    2971    1872 
   Size:       1099    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2506    2672    2711    3748    4649    4911 
   Size:        197     926     563     166      39    1037     901     262

Cuts at:   4911     820 
   Size:       1553

  Fragments arranged by size: 

                1553    1037     926     901     563     262     197     166
                  39
 
SnaBI TAC'GTA

Cuts at:    493    3435     493 
   Size:       2942    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5644
 
SphI G_CATG'C

Cuts at:   1132    2470    3002    1132 
   Size:       1338     532    3774

  Fragments arranged by size: 

                3774    1338     532
 
SspI AAT'ATT

Cuts at:      5      52    3568       5 
   Size:         47    3516    2081

  Fragments arranged by size: 

                3516    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5644
 
StyI C'CwwG_G

Cuts at:    513    1065    3083     513 
   Size:        552    2018    3074

  Fragments arranged by size: 

                3074    2018     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3671    3688    3841    4026    4471     325 
   Size:       1255    2091      17     153     185     445    1498

  Fragments arranged by size: 

                2091    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2187    2335    2680 
   Size:        274      80      33      51    1443     210     148     345

Cuts at:   2680    3890    5602      96 
   Size:       1210    1712     138

  Fragments arranged by size: 

                1712    1443    1210     345     274     210     148     138
                  80      51      33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5604    5636    5604 
   Size:         32    5612
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5644
 
XbaI T'CTAG_A

Cuts at:   2980    2980 
   Size:       5644
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2047    2776    2047 
   Size:        729    4915
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5644
 
XmaI C'CCGG_G

Cuts at:   1870    2969    1870 
   Size:       1099    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3773    3773 
   Size:       5644
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3452     276 
   Size:         53      83     186     936    1918    2468

  Fragments arranged by size: 

                2468    1918     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BpmI
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstYI     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI     DrdI     EaeI     EagI     EarI     EciI  Eco57MI    EcoRI
     FspI    HaeII    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI
     MluI     MmeI     MscI     MslI   MspA1I     NcoI     NdeI     NheI
     NotI     NspI     PasI     PciI    PflMI     PmeI     PpiI    PshAI
     PsiI     PstI     PvuI     SalI     SbfI     ScaI     SfcI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SspI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI
    BbvCI    BfrBI     BplI   Bpu10I    BsiWI    BsmBI    BstXI  BstZ17I
   Bsu36I   DraIII  EcoICRI    EcoNI EcoO109I    EcoRV     FalI     FseI
    FspAI     KasI     NaeI     NarI   NgoMIV     NruI     NsiI     PacI
     PfoI     PmlI    PpuMI   PspOMI    PspXI     PsrI    PvuII    RsrII
     SacI    SacII    SanDI     SapI    SexAI     SfiI     SfoI     SrfI
     SwaI
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