################################## # NO CUT ENZYMES ( 150 enzymes) # ################################## Acc65I AccI AccIII AclI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaII AviII AvrII BanI BbeI BbrPI BbsI BcgI BciVI BcnI BfrI BglII BlnI BpuAI BsaBI BsaI BsaWI BseAI BsgI BsiEI BsiWI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraII DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRV EcoT22I FseI FspI HaeII HapII HgaI HincII HindII HindIII HpaI HpaII KasI KpnI KspI MamI MfeI MluI MroI MseI MspI MunI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI SspI SwaI TaqI Tru9I Tth111I TthHB8I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AatII 586 GACGTC GACGT^C AccII 213 CGCG CG^CG AcsI 699 GAATTC [GA]^AATT[CT] AcyI 586 GACGTC G[GA]^CG[CT]C ApoI 699 GAATTC [GA]^AATT[CT] AspHI 3 GTGCTC G[AT]GC[AT]^C AvaI 798 CCCGAG C^[CT]CG[GA]G BamHI 308 GGATCC G^GATCC BbvCI 343 GCTGAGG CCTCAGC(-5/-2) BclI 749 TGATCA T^GATCA BfaI 527 CTAG C^TAG BglI 454 GCCTGCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 817 GCTGAGC GC^T[ACGT]AGC Bpu1102I 817 GCTGAGC GC^T[ACGT]AGC BsaAI 609 CACGTA [CT]AC^GT[GA] BsaHI 586 GACGTC G[GA]^CG[CT]C BsiHKAI 3 GTGCTC G[AT]GC[AT]^C BsmBI 588 CGTCTC CGTCTC(1/5) BsmFI 60 GGGAC GGGAC(10/14) BsmI 138 GAATGC GAATGC(1/-1) BsoBI 798 CCCGAG C^[CT]CG[GA]G BspMI 733 ACCTGC ACCTGC(4/8) BstUI 213 CGCG CG^CG BstXI 267 CCAGGTCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 817 GCTGAGC GC^T[ACGT]AGC CfoI 214 GCGC GCG^C DsaI 365 CCATGG C^C[GA][CT]GG EarI 766 CTCTTC CTCTTC(1/4) EcoRI 699 GAATTC G^AATTC FbaI 749 TGATCA T^GATCA HhaI 214 GCGC GCG^C Hin1I 586 GACGTC G[GA]^CG[CT]C HinP1I 214 GCGC G^CGC Ksp632I 766 CTCTTC CTCTTC(1/4) MaeI 527 CTAG C^TAG MvnI 213 CGCG CG^CG NcoI 365 CCATGG C^CATGG NlaIV 308 GGATCC GG[ACGT]^[ACGT]CC PflMI 438 CCAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PvuII 815 CAGCTG CAG^CTG SapI 765 GCTCTTC GCTCTTC(1/4) SphI 81 GCATGC GCATG^C StuI 722 AGGCCT AGG^CCT Tsp509I 700 AATT ^AATT Van91I 438 CCAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 251 CCAAGATGCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AlwNI 439 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG 885 CAGTCTCTG BalI 711 TGGCCA TGG^CCA 728 TGGCCA BanII 169 GAGCCC G[GA]GC[CT]^C 911 GGGCTC BstYI 222 GGATCT [GA]^GATC[CT] 308 GGATCC Cfr13I 126 GGCCC G^G[ACGT]CC 264 GGGCC EaeI 711 TGGCCA [CT]^GGCC[GA] 728 TGGCCA EcoT14I 365 CCATGG C^C[AT][AT]GG 725 CCTTGG FauI 173 CCCGC CCCGC(4/6) 411 CCCGC MaeII 587 ACGT A^CGT 610 ACGT MflI 222 GGATCT [GA]^GATC[CT] 308 GGATCC MluNI 711 TGGCCA TGG^CCA 728 TGGCCA MscI 711 TGGCCA TGG^CCA 728 TGGCCA NspI 81 GCATGC [GA]CATG^[CT] 791 ACATGC PleI 62 GACTC GAGTC(4/5) 555 GACTC Sau96I 126 GGCCC G^G[ACGT]CC 264 GGGCC StyI 365 CCATGG C^C[AT][AT]GG 725 CCTTGG TfiI 12 GAATC G^A[AT]TC 27 GAATC XhoII 222 GGATCT [GA]^GATC[CT] 308 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 44 enzymes) # ######################################### AciI AfaI AluI AlwI BbvI BmyI BpmI BsaJI BseRI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I DdeI DpnII EcoRII Fnu4HI FokI HaeIII HinfI HphI ItaI MaeIII MboI MboII MnlI MslI MspA1I MvaI MwoI NdeII NlaIII RsaI Sau3AI ScrFI SfaNI TseI Tsp45I TspRI