Restriction map for pFN21AE6050
 (Circular) (Six-base) MAPSORT of: pFN21AE6050.seq  Check: 6865  from: 1  to: 5659



pFN21AE6050  5659 bp

 With 182 enzymes: SgfI * 

                        December 8, 2014 13:50  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   2747    2747 
   Size:       5659
 
AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    2595    3469     278 
   Size:         53      83     186     936    1059     874    2468

  Fragments arranged by size: 

                2468    1059     936     874     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    3002    1937 
   Size:       1065    4594
 
Acc65I G'GTAC_C

Cuts at:   2980    2980 
   Size:       5659
 
AclI AA'CG_TT

Cuts at:   1563    3786    4159    1563 
   Size:       2223     373    3063

  Fragments arranged by size: 

                3063    2223     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2594    3722    5106    5374    1632 
   Size:        962    1128    1384     268    1917

  Fragments arranged by size: 

                1917    1384    1128     962     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5462
 
AflIII A'CryG_T

Cuts at:   1203    4558    5535    1203 
   Size:       3355     977    1327

  Fragments arranged by size: 

                3355    1327     977
 
AgeI A'CCGG_T

Cuts at:   4490    4490 
   Size:       5659
 
AhdI GACnn_n'nnGTC

Cuts at:   4388    4388 
   Size:       5659
 
AleI CACnn'nnGTG

Cuts at:   1166    2867    1166 
   Size:       1701    3958
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    2651    1184 
   Size:       1467    4192
 
AlwNI CAG_nnn'CTG

Cuts at:   2449    2895    3402    4974    2449 
   Size:        446     507    1572    3134

  Fragments arranged by size: 

                3134    1572     507     446
 
ApaLI G'TGCA_C

Cuts at:   3716    4872    3716 
   Size:       1156    4503
 
ApoI r'AATT_y

Cuts at:   1493    2704    2968    3194    3346    5455    1493 
   Size:       1211     264     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1211     264     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    4213     160 
   Size:        872    3181    1606

  Fragments arranged by size: 

                3181    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5659
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2803    2984    1870 
   Size:         73     860     181    4545

  Fragments arranged by size: 

                4545     860     181      73
 
BamHI G'GATC_C

Cuts at:   2313    2989    3356    2313 
   Size:        676     367    4616

  Fragments arranged by size: 

                4616     676     367
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2980    4435     618 
   Size:        325     217     627    1193    1455    1842

  Fragments arranged by size: 

                1842    1455    1193     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2178    2920    2978    1391 
   Size:        787     742      58    4072

  Fragments arranged by size: 

                4072     787     742      58
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    4688
 
BbvCI CC'TCA_GC

Cuts at:   2349    2349 
   Size:       5659
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3850    3884    1903 
   Size:         34    1913      34    3678

  Fragments arranged by size: 

                3678    1913      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3551    4761    3551 
   Size:       1210    4449
 
BclI T'GATC_A

Cuts at:   1548    2754    1548 
   Size:       1206    4453
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2465    4270     136 
   Size:        107     122      71     631     605     793    1805    1525

  Fragments arranged by size: 

                1805    1525     793     631     605     122     107      71
            
 
BglII A'GATC_T

Cuts at:   5654    5654 
   Size:       5659
 
BlpI GC'TnA_GC

Cuts at:   2823    3076    2823 
   Size:        253    5406
 
Bme1580I G_kGCm'C

Cuts at:   1790    2256    3720    4876    1790 
   Size:        466    1464    1156    2573

  Fragments arranged by size: 

                2573    1464    1156     466
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5659
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2148    3390    4348 
   Size:        416      53      83     399      24     731    1242     958

Cuts at:   4348     442 
   Size:       1753

  Fragments arranged by size: 

                1753    1242     958     731     416     399      83      53
                  24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5659
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2301    2364    2647    4319    1239 
   Size:       1062      63     283    1672    2579

  Fragments arranged by size: 

                2579    1672    1062     283      63
 
Bpu10I CC'TnA_GC

Cuts at:   2011    2349    2011 
   Size:        338    5321
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3784    4649    4947    3784 
   Size:        865     298    4496

  Fragments arranged by size: 

                4496     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4322     882 
   Size:        617    2823    2219

  Fragments arranged by size: 

                2823    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    2616    3450     493 
   Size:        711    1412     834    2702

  Fragments arranged by size: 

                2702    1412     834     711
 
BsaBI GATnn'nnATC

Cuts at:   5653    5653 
   Size:       5659
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2592    3466    3848    4483 
   Size:         53      83     186     936    1059     874     382     635

Cuts at:   4483     275 
   Size:       1451

  Fragments arranged by size: 

                1451    1059     936     874     635     382     186      83
                  53
 
BsaWI w'CCGG_w

Cuts at:   4092    4490    4764    4911    5322    4092 
   Size:        398     274     147     411    4429

  Fragments arranged by size: 

                4429     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2337    2367    1178 
   Size:         30     165      30     934      30    4470

  Fragments arranged by size: 

                4470     934     165      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2087    2141    2150    2734    2938    1186 
   Size:        901      54       9     584     204    3907

  Fragments arranged by size: 

                3907     901     584     204      54       9
 
BseYI C'CCAG_C

Cuts at:   2463    4862    5358    2463 
   Size:       2399     496    2764

  Fragments arranged by size: 

                2764    2399     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    5086
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3109    3870    4898    1540 
   Size:        275     186    1108     761    1028    2301

  Fragments arranged by size: 

                2301    1108    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2012    3720    3805    4876    2012 
   Size:       1708      85    1071    2795

  Fragments arranged by size: 

                2795    1708    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2149    3170    3263    2149 
   Size:       1021      93    4545

  Fragments arranged by size: 

                4545    1021      93
 
BsmBI CGTCTCn'nnnn_

Cuts at:   2599    2599 
   Size:       5659
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2012    2178    2256    2920    2978    3720    3805 
   Size:        399     222     166      78     664      58     742      85

Cuts at:   3805    4876    1391 
   Size:       1071    2174

  Fragments arranged by size: 

                2174    1071     742     664     399     222     166      85
                  78      58
 
BspEI T'CCGG_A

Cuts at:   5322    5322 
   Size:       5659
 
BspHI T'CATG_A

Cuts at:   3548    5278    3548 
   Size:       1730    3929
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2747    3014     844 
   Size:       1903     267    3489

  Fragments arranged by size: 

                3489    1903     267
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3546    1394 
   Size:        429    1723    3507

  Fragments arranged by size: 

                3507    1723     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    4154    4328      65 
   Size:       1183    2906     174    1396

  Fragments arranged by size: 

                2906    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4303    4490    1700 
   Size:        110    2493     187    2869

  Fragments arranged by size: 

                2869    2493     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5659
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5659
 
BssSI C'ACGA_G

Cuts at:   3719    4731    3719 
   Size:       1012    4647
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5659
 
BstBI TT'CG_AA

Cuts at:   4463    4463 
   Size:       5659
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5659
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2279    2279 
   Size:       5659
 
BstYI r'GATC_y

Cuts at:   1972    2227    2313    2989    3356    3741    3758    5210    5296 
   Size:        255      86     676     367     385      17    1452      86

Cuts at:   5296    5308    5654    1972 
   Size:         12     346    1977

  Fragments arranged by size: 

                1977    1452     676     385     367     346     255      86
                  86      17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2370     513 
   Size:        552    1305    3802

  Fragments arranged by size: 

                3802    1305     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4920

  Fragments arranged by size: 

                4920     683      56
 
BtsI GCAGTG_nn'

Cuts at:   3175    3969    3989    3175 
   Size:        794      20    4845

  Fragments arranged by size: 

                4845     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5659
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5624
 
DraI TTT'AAA

Cuts at:   2963    2963 
   Size:       5659
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4666     809 
   Size:        507    3350    1802

  Fragments arranged by size: 

                3350    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2716    2733    3106 
   Size:         54    1425      50     275      18     886      17     373

Cuts at:   3106    3995    5624       8 
   Size:        889    1629      43

  Fragments arranged by size: 

                1629    1425     889     886     373     275      54      50
                  43      18      17
 
EagI C'GGCC_G

Cuts at:   1537    1812    3106    1537 
   Size:        275    1294    4090

  Fragments arranged by size: 

                4090    1294     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2777    3589    1353 
   Size:       1424     812    3423

  Fragments arranged by size: 

                3423    1424     812
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4246    4620    4766     387 
   Size:       1064     138    2657     374     146    1280

  Fragments arranged by size: 

                2657    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2301    2364    2594    2647    3722    4319    5106 
   Size:        393     669      63     230      53    1075     597     787

Cuts at:   5106    5374    1239 
   Size:        268    1524

  Fragments arranged by size: 

                1524    1075     787     669     597     393     268     230
                  63      53
 
EcoRI G'AATT_C

Cuts at:   2704    2968    3346    2704 
   Size:        264     378    5017

  Fragments arranged by size: 

                5017     378     264
 
FspI TGC'GCA

Cuts at:   4165    4165 
   Size:       5659
 
HaeII r_GCGC'y

Cuts at:   1678    4806    1678 
   Size:       3128    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    4300    4332    4374    4406    1373 
   Size:         32     135      32    2728      32      42      32    2626

  Fragments arranged by size: 

                2728    2626     135      42      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    3003    3249     669 
   Size:        988     281    1065     246    3079

  Fragments arranged by size: 

                3079    1065     988     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5659
 
HpaI GTT'AAC

Cuts at:   3249    3249 
   Size:       5659
 
KpnI G_GTAC'C

Cuts at:   2984    2984 
   Size:       5659
 
MfeI C'AATT_G

Cuts at:   3258    3258 
   Size:       5659
 
MluI A'CGCG_T

Cuts at:   5535    5535 
   Size:       5659
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4773    4957    1305 
   Size:       3468     184    2007

  Fragments arranged by size: 

                3468    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2718    2735      10 
   Size:         54    1425     343     886      17    2934

  Fragments arranged by size: 

                2934    1425     886     343      54      17
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2192    2507    2633    2795    2837    2867    3617 
   Size:        648    1026     315     126     162      42      30     750

Cuts at:   3617    3976    4135     518 
   Size:        359     159    2042

  Fragments arranged by size: 

                2042    1026     750     648     359     315     162     159
                 126      42      30
 
MspA1I CmG'CkG

Cuts at:   1606    2419    2714    2822    3075    3752    4900    5145    1606 
   Size:        813     295     108     253     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     813     677     295     253     245     108
            
 
NcoI C'CATG_G

Cuts at:    513    1065    2370     513 
   Size:        552    1305    3802

  Fragments arranged by size: 

                3802    1305     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5659
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5659
 
NotI GC'GGCC_GC

Cuts at:   3106    3106 
   Size:       5659
 
NspI r_CATG'y

Cuts at:   1132    1229    2090    2800    3017    4562    1132 
   Size:         97     861     710     217    1545    2229

  Fragments arranged by size: 

                2229    1545     861     710     217      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5659
 
PciI A'CATG_T

Cuts at:   4558    4558 
   Size:       5659
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2449    1108 
   Size:       1341    4318
 
PmeI GTTT'AAAC

Cuts at:   2963    2963 
   Size:       5659
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3728    3760    5241    5273    3728 
   Size:         32    1481      32    4114

  Fragments arranged by size: 

                4114    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5659
 
PsiI TTA'TAA

Cuts at:   3229    3229 
   Size:       5659
 
PstI C_TGCA'G

Cuts at:    830    3011     830 
   Size:       2181    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5659
 
PvuII CAG'CTG

Cuts at:   2822    2822 
   Size:       5659
 
SalI G'TCGA_C

Cuts at:   1936    3001    1936 
   Size:       1065    4594
 
SapI GCTCTTCn'nnn_

Cuts at:   2777    2777 
   Size:       5659
 
SbfI CC_TGCA'GG

Cuts at:   3011    3011 
   Size:       5659
 
ScaI AGT'ACT

Cuts at:   3907    5619    3907 
   Size:       1712    3947
 
SfcI C'TryA_G

Cuts at:    826    1045    3007    4142    4823    5014     826 
   Size:        219    1962    1135     681     191    1471

  Fragments arranged by size: 

                1962    1471    1135     681     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5659
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5659
 
SmaI CCC'GGG

Cuts at:   1872    2986    1872 
   Size:       1114    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3763    4664    4926     820 
   Size:        197     926    1820     901     262    1553

  Fragments arranged by size: 

                1820    1553     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3450     493 
   Size:       2957    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5659
 
SphI G_CATG'C

Cuts at:   1132    2090    3017    1132 
   Size:        958     927    3774

  Fragments arranged by size: 

                3774     958     927
 
SspI AAT'ATT

Cuts at:      5      52    3583       5 
   Size:         47    3531    2081

  Fragments arranged by size: 

                3531    2081      47
 
StuI AGG'CCT

Cuts at:   1517    2729    1517 
   Size:       1212    4447
 
StyI C'CwwG_G

Cuts at:    513    1065    2370    2730    3098     513 
   Size:        552    1305     360     368    3074

  Fragments arranged by size: 

                3074    1305     552     368     360
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2397    3686    3703    3856    4041    4486     325 
   Size:       1255     817    1289      17     153     185     445    1498

  Fragments arranged by size: 

                1498    1289    1255     817     445     185     153      17
            
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2196    3905    5617 
   Size:        274      80      33      51    1443     219    1709    1712

Cuts at:   5617      96 
   Size:        138

  Fragments arranged by size: 

                1712    1709    1443     274     219     138      80      51
                  33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5619    5651    5619 
   Size:         32    5627
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5659
 
XbaI T'CTAG_A

Cuts at:   2995    2995 
   Size:       5659
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2263    2263 
   Size:       5659
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5659
 
XmaI C'CCGG_G

Cuts at:   1870    2984    1870 
   Size:       1114    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3788    3788 
   Size:       5659
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    2593    3467     276 
   Size:         53      83     186     936    1059     874    2468

  Fragments arranged by size: 

                2468    1059     936     874     186      83      53
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbsI    BbvCI     BcgI
    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI
    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI     EaeI
     EagI     EarI     EciI  Eco57MI    EcoRI     FspI    HaeII    Hin4I
   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MscI
     MslI   MspA1I     NcoI     NdeI     NheI     NotI     NspI     PasI
     PciI    PflMI     PmeI     PpiI    PshAI     PsiI     PstI     PvuI
    PvuII     SalI     SapI     SbfI     ScaI     SfcI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SspI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI

 Enzymes that do not cut: 

     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI    BfrBI
     BplI    BsiWI  BstZ17I   Bsu36I   DraIII  EcoICRI    EcoNI EcoO109I
    EcoRV     FalI     FseI    FspAI     KasI     NaeI     NarI   NgoMIV
     NruI     NsiI     PacI     PfoI     PmlI    PpuMI   PspOMI    PspXI
     PsrI    RsrII     SacI    SacII    SanDI    SexAI     SfiI     SfoI
     SrfI     SwaI
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