(Circular) (Six-base) MAPSORT of: pFN21AE6050.seq Check: 6865 from: 1 to: 5659 pFN21AE6050 5659 bp With 182 enzymes: SgfI * December 8, 2014 13:50 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2747 2747 Size: 5659 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2595 3469 278 Size: 53 83 186 936 1059 874 2468 Fragments arranged by size: 2468 1059 936 874 186 83 53 AccI GT'mk_AC Cuts at: 1937 3002 1937 Size: 1065 4594 Acc65I G'GTAC_C Cuts at: 2980 2980 Size: 5659 AclI AA'CG_TT Cuts at: 1563 3786 4159 1563 Size: 2223 373 3063 Fragments arranged by size: 3063 2223 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2594 3722 5106 5374 1632 Size: 962 1128 1384 268 1917 Fragments arranged by size: 1917 1384 1128 962 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5462 AflIII A'CryG_T Cuts at: 1203 4558 5535 1203 Size: 3355 977 1327 Fragments arranged by size: 3355 1327 977 AgeI A'CCGG_T Cuts at: 4490 4490 Size: 5659 AhdI GACnn_n'nnGTC Cuts at: 4388 4388 Size: 5659 AleI CACnn'nnGTG Cuts at: 1166 2867 1166 Size: 1701 3958 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2651 1184 Size: 1467 4192 AlwNI CAG_nnn'CTG Cuts at: 2449 2895 3402 4974 2449 Size: 446 507 1572 3134 Fragments arranged by size: 3134 1572 507 446 ApaLI G'TGCA_C Cuts at: 3716 4872 3716 Size: 1156 4503 ApoI r'AATT_y Cuts at: 1493 2704 2968 3194 3346 5455 1493 Size: 1211 264 226 152 2109 1697 Fragments arranged by size: 2109 1697 1211 264 226 152 AseI AT'TA_AT Cuts at: 160 1032 4213 160 Size: 872 3181 1606 Fragments arranged by size: 3181 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5659 AvaI C'yCGr_G Cuts at: 1870 1943 2803 2984 1870 Size: 73 860 181 4545 Fragments arranged by size: 4545 860 181 73 BamHI G'GATC_C Cuts at: 2313 2989 3356 2313 Size: 676 367 4616 Fragments arranged by size: 4616 676 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2980 4435 618 Size: 325 217 627 1193 1455 1842 Fragments arranged by size: 1842 1455 1193 627 325 217 BanII G_rGCy'C Cuts at: 1391 2178 2920 2978 1391 Size: 787 742 58 4072 Fragments arranged by size: 4072 787 742 58 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4688 BbvCI CC'TCA_GC Cuts at: 2349 2349 Size: 5659 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3850 3884 1903 Size: 34 1913 34 3678 Fragments arranged by size: 3678 1913 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3551 4761 3551 Size: 1210 4449 BclI T'GATC_A Cuts at: 1548 2754 1548 Size: 1206 4453 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2465 4270 136 Size: 107 122 71 631 605 793 1805 1525 Fragments arranged by size: 1805 1525 793 631 605 122 107 71 BglII A'GATC_T Cuts at: 5654 5654 Size: 5659 BlpI GC'TnA_GC Cuts at: 2823 3076 2823 Size: 253 5406 Bme1580I G_kGCm'C Cuts at: 1790 2256 3720 4876 1790 Size: 466 1464 1156 2573 Fragments arranged by size: 2573 1464 1156 466 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5659 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2148 3390 4348 Size: 416 53 83 399 24 731 1242 958 Cuts at: 4348 442 Size: 1753 Fragments arranged by size: 1753 1242 958 731 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5659 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2301 2364 2647 4319 1239 Size: 1062 63 283 1672 2579 Fragments arranged by size: 2579 1672 1062 283 63 Bpu10I CC'TnA_GC Cuts at: 2011 2349 2011 Size: 338 5321 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3784 4649 4947 3784 Size: 865 298 4496 Fragments arranged by size: 4496 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4322 882 Size: 617 2823 2219 Fragments arranged by size: 2823 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2616 3450 493 Size: 711 1412 834 2702 Fragments arranged by size: 2702 1412 834 711 BsaBI GATnn'nnATC Cuts at: 5653 5653 Size: 5659 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2592 3466 3848 4483 Size: 53 83 186 936 1059 874 382 635 Cuts at: 4483 275 Size: 1451 Fragments arranged by size: 1451 1059 936 874 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4092 4490 4764 4911 5322 4092 Size: 398 274 147 411 4429 Fragments arranged by size: 4429 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2337 2367 1178 Size: 30 165 30 934 30 4470 Fragments arranged by size: 4470 934 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2087 2141 2150 2734 2938 1186 Size: 901 54 9 584 204 3907 Fragments arranged by size: 3907 901 584 204 54 9 BseYI C'CCAG_C Cuts at: 2463 4862 5358 2463 Size: 2399 496 2764 Fragments arranged by size: 2764 2399 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5086 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3109 3870 4898 1540 Size: 275 186 1108 761 1028 2301 Fragments arranged by size: 2301 1108 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2012 3720 3805 4876 2012 Size: 1708 85 1071 2795 Fragments arranged by size: 2795 1708 1071 85 BsmI GAATG_Cn' Cuts at: 2149 3170 3263 2149 Size: 1021 93 4545 Fragments arranged by size: 4545 1021 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2599 2599 Size: 5659 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2012 2178 2256 2920 2978 3720 3805 Size: 399 222 166 78 664 58 742 85 Cuts at: 3805 4876 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 664 399 222 166 85 78 58 BspEI T'CCGG_A Cuts at: 5322 5322 Size: 5659 BspHI T'CATG_A Cuts at: 3548 5278 3548 Size: 1730 3929 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2747 3014 844 Size: 1903 267 3489 Fragments arranged by size: 3489 1903 267 BsrBI CCG'CTC Cuts at: 1394 1823 3546 1394 Size: 429 1723 3507 Fragments arranged by size: 3507 1723 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4154 4328 65 Size: 1183 2906 174 1396 Fragments arranged by size: 2906 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4303 4490 1700 Size: 110 2493 187 2869 Fragments arranged by size: 2869 2493 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5659 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5659 BssSI C'ACGA_G Cuts at: 3719 4731 3719 Size: 1012 4647 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5659 BstBI TT'CG_AA Cuts at: 4463 4463 Size: 5659 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5659 BstXI CCAn_nnnn'nTGG Cuts at: 2279 2279 Size: 5659 BstYI r'GATC_y Cuts at: 1972 2227 2313 2989 3356 3741 3758 5210 5296 Size: 255 86 676 367 385 17 1452 86 Cuts at: 5296 5308 5654 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 676 385 367 346 255 86 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2370 513 Size: 552 1305 3802 Fragments arranged by size: 3802 1305 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4920 Fragments arranged by size: 4920 683 56 BtsI GCAGTG_nn' Cuts at: 3175 3969 3989 3175 Size: 794 20 4845 Fragments arranged by size: 4845 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5659 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5624 DraI TTT'AAA Cuts at: 2963 2963 Size: 5659 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4666 809 Size: 507 3350 1802 Fragments arranged by size: 3350 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2716 2733 3106 Size: 54 1425 50 275 18 886 17 373 Cuts at: 3106 3995 5624 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 886 373 275 54 50 43 18 17 EagI C'GGCC_G Cuts at: 1537 1812 3106 1537 Size: 275 1294 4090 Fragments arranged by size: 4090 1294 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2777 3589 1353 Size: 1424 812 3423 Fragments arranged by size: 3423 1424 812 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4246 4620 4766 387 Size: 1064 138 2657 374 146 1280 Fragments arranged by size: 2657 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2301 2364 2594 2647 3722 4319 5106 Size: 393 669 63 230 53 1075 597 787 Cuts at: 5106 5374 1239 Size: 268 1524 Fragments arranged by size: 1524 1075 787 669 597 393 268 230 63 53 EcoRI G'AATT_C Cuts at: 2704 2968 3346 2704 Size: 264 378 5017 Fragments arranged by size: 5017 378 264 FspI TGC'GCA Cuts at: 4165 4165 Size: 5659 HaeII r_GCGC'y Cuts at: 1678 4806 1678 Size: 3128 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4300 4332 4374 4406 1373 Size: 32 135 32 2728 32 42 32 2626 Fragments arranged by size: 2728 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3003 3249 669 Size: 988 281 1065 246 3079 Fragments arranged by size: 3079 1065 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5659 HpaI GTT'AAC Cuts at: 3249 3249 Size: 5659 KpnI G_GTAC'C Cuts at: 2984 2984 Size: 5659 MfeI C'AATT_G Cuts at: 3258 3258 Size: 5659 MluI A'CGCG_T Cuts at: 5535 5535 Size: 5659 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4773 4957 1305 Size: 3468 184 2007 Fragments arranged by size: 3468 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2718 2735 10 Size: 54 1425 343 886 17 2934 Fragments arranged by size: 2934 1425 886 343 54 17 MslI CAynn'nnrTG Cuts at: 518 1166 2192 2507 2633 2795 2837 2867 3617 Size: 648 1026 315 126 162 42 30 750 Cuts at: 3617 3976 4135 518 Size: 359 159 2042 Fragments arranged by size: 2042 1026 750 648 359 315 162 159 126 42 30 MspA1I CmG'CkG Cuts at: 1606 2419 2714 2822 3075 3752 4900 5145 1606 Size: 813 295 108 253 677 1148 245 2120 Fragments arranged by size: 2120 1148 813 677 295 253 245 108 NcoI C'CATG_G Cuts at: 513 1065 2370 513 Size: 552 1305 3802 Fragments arranged by size: 3802 1305 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5659 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5659 NotI GC'GGCC_GC Cuts at: 3106 3106 Size: 5659 NspI r_CATG'y Cuts at: 1132 1229 2090 2800 3017 4562 1132 Size: 97 861 710 217 1545 2229 Fragments arranged by size: 2229 1545 861 710 217 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5659 PciI A'CATG_T Cuts at: 4558 4558 Size: 5659 PflMI CCAn_nnn'nTGG Cuts at: 1108 2449 1108 Size: 1341 4318 PmeI GTTT'AAAC Cuts at: 2963 2963 Size: 5659 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3728 3760 5241 5273 3728 Size: 32 1481 32 4114 Fragments arranged by size: 4114 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5659 PsiI TTA'TAA Cuts at: 3229 3229 Size: 5659 PstI C_TGCA'G Cuts at: 830 3011 830 Size: 2181 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5659 PvuII CAG'CTG Cuts at: 2822 2822 Size: 5659 SalI G'TCGA_C Cuts at: 1936 3001 1936 Size: 1065 4594 SapI GCTCTTCn'nnn_ Cuts at: 2777 2777 Size: 5659 SbfI CC_TGCA'GG Cuts at: 3011 3011 Size: 5659 ScaI AGT'ACT Cuts at: 3907 5619 3907 Size: 1712 3947 SfcI C'TryA_G Cuts at: 826 1045 3007 4142 4823 5014 826 Size: 219 1962 1135 681 191 1471 Fragments arranged by size: 1962 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5659 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5659 SmaI CCC'GGG Cuts at: 1872 2986 1872 Size: 1114 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3763 4664 4926 820 Size: 197 926 1820 901 262 1553 Fragments arranged by size: 1820 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3450 493 Size: 2957 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5659 SphI G_CATG'C Cuts at: 1132 2090 3017 1132 Size: 958 927 3774 Fragments arranged by size: 3774 958 927 SspI AAT'ATT Cuts at: 5 52 3583 5 Size: 47 3531 2081 Fragments arranged by size: 3531 2081 47 StuI AGG'CCT Cuts at: 1517 2729 1517 Size: 1212 4447 StyI C'CwwG_G Cuts at: 513 1065 2370 2730 3098 513 Size: 552 1305 360 368 3074 Fragments arranged by size: 3074 1305 552 368 360 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2397 3686 3703 3856 4041 4486 325 Size: 1255 817 1289 17 153 185 445 1498 Fragments arranged by size: 1498 1289 1255 817 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2196 3905 5617 Size: 274 80 33 51 1443 219 1709 1712 Cuts at: 5617 96 Size: 138 Fragments arranged by size: 1712 1709 1443 274 219 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5619 5651 5619 Size: 32 5627 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5659 XbaI T'CTAG_A Cuts at: 2995 2995 Size: 5659 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2263 2263 Size: 5659 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5659 XmaI C'CCGG_G Cuts at: 1870 2984 1870 Size: 1114 4545 XmnI GAAnn'nnTTC Cuts at: 3788 3788 Size: 5659 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2593 3467 276 Size: 53 83 186 936 1059 874 2468 Fragments arranged by size: 2468 1059 936 874 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI