################################## # NO CUT ENZYMES ( 143 enzymes) # ################################## AatII AccI AccII AccIII AclI AcyI AflII AgeI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I AspHI AspI AviII AvrII BalI BamHI BanII BbeI BbrPI BcgI BfrI BglI BlnI BmyI BsaBI BsaHI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BsmI Bsp1286I Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrGI BsrI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI EarI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRV EcoT22I FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HpaI KasI Ksp632I KspI MamI MfeI MluI MluNI MroI MscI MseI MspA1I MunI MvnI NarI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI PvuII RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TaqI Tru9I Tth111I TthHB8I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### Acc65I 395 GGTACC G^GTACC AcsI 334 GAATTC [GA]^AATT[CT] AhdI 253 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApoI 334 GAATTC [GA]^AATT[CT] Asp718I 395 GGTACC G^GTACC AspEI 253 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaII 252 GGACC G^G[AT]CC BanI 395 GGTACC G^G[CT][GA]CC BbvCI 694 GCTGAGG CCTCAGC(-5/-2) BciVI 210 GGATAC GTATCC(6/5) BclI 959 TGATCA T^GATCA BfaI 916 CTAG C^TAG BglII 302 AGATCT A^GATCT BlpI 189 GCTCAGC GC^T[ACGT]AGC Bpu1102I 189 GCTCAGC GC^T[ACGT]AGC BsaAI 720 TACGTG [CT]AC^GT[GA] BseRI 403 CTCCTC GAGGAG(10/8) BsgI 293 GTGCAG GTGCAG(16/14) BsmFI 235 GTCCC GGGAC(10/14) BsrFI 701 GCCGGC [GA]^CCGG[CT] BstAPI 416 GCAGGGTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 101 CCATCTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 189 GCTCAGC GC^T[ACGT]AGC CfoI 705 GCGC GCG^C Cfr10I 701 GCCGGC [GA]^CCGG[CT] DraII 251 AGGACCT [GA]G^G[ACGT]CC[CT] Eam1105I 253 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 258 CCTGTCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 251 AGGACCT [GA]G^G[ACGT]CC[CT] EcoRI 334 GAATTC G^AATTC FauI 161 CCCGC CCCGC(4/6) FbaI 959 TGATCA T^GATCA HhaI 705 GCGC GCG^C HinP1I 705 GCGC G^CGC KpnI 395 GGTACC GGTAC^C MaeI 916 CTAG C^TAG NaeI 701 GCCGGC GCC^GGC NgoMIV 701 GCCGGC G^CCGGC PflMI 732 CCACATTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 251 AGGACCT [GA]G^G[AT]CC[CT] PshAI 480 GACTCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 896 CTGCAG CTGCA^G ScaI 912 AGTACT AGT^ACT SmaI 73 CCCGGG CCC^GGG SmlI 876 CTTGAG C^T[CT][GA]AG StuI 48 AGGCCT AGG^CCT TfiI 138 GATTC G^A[AT]TC Tsp509I 335 AATT ^AATT TspRI 231 CACTG CA[GC]TG[ACGT][ACGT]^ Van91I 732 CCACATTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 73 CCCGGG C^CCGGG XmaI 73 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AflIII 575 ACACGT A^C[GA][CT]GT 721 ACGTGT AlwI 512 GATCC GGATC(4/5) 543 GATCC AvaI 73 CCCGGG C^[CT]CG[GA]G 515 CCCGAG BbsI 14 GTCTTC GAAGAC(2/6) 94 GTCTTC BcnI 73 CCCGG CC^[GC]GG 74 CCGGG BpuAI 14 GTCTTC GAAGAC(2/6) 94 GTCTTC BsaI 589 GAGACC GGTCTC(1/5) 349 GGTCTC BsoBI 73 CCCGGG C^[CT]CG[GA]G 515 CCCGAG BspHI 134 TCATGA T^CATGA 407 TCATGA Cfr13I 252 GGACC G^G[ACGT]CC 699 GGGCC EcoT14I 263 CCAAGG C^C[AT][AT]GG 461 CCTTGG HapII 74 CCGG C^CGG 702 CCGG HpaII 74 CCGG C^CGG 702 CCGG MaeIII 487 GTCAC ^GT[ACGT]AC 579 GTCAC MspI 74 CCGG C^CGG 702 CCGG NciI 73 CCCGG CC^[GC]GG 74 CCGGG PleI 157 GACTC GAGTC(4/5) 480 GACTC RcaI 134 TCATGA T^CATGA 407 TCATGA Sau96I 252 GGACC G^G[ACGT]CC 699 GGGCC StyI 263 CCAAGG C^C[AT][AT]GG 461 CCTTGG Tsp45I 487 GTCAC ^GT[GC]AC 579 GTCAC XcmI 101 CCATCTTTGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 275 CCATTTCCTTCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 41 enzymes) # ######################################### AciI AfaI AluI BbvI BpmI BsaJI BsiYI BslI BsmAI BssKI BstF5I BstNI BstYI Cac8I DdeI DpnII EcoRII Fnu4HI FokI HaeIII HinfI HphI ItaI MaeII MboI MboII MflI MnlI MslI MvaI MwoI NdeII NlaIII NlaIV RsaI Sau3AI ScrFI SfaNI SfcI TseI XhoII