################################## # NO CUT ENZYMES ( 167 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BamHI BbeI BbrPI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaHI BsaI BsaJI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrFI BsrGI BsrI BssHII BssSI BstAPI BstBI BstEII BstPI BstUI BstYI Bsu36I CelII Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRV EcoT14I EcoT22I FbaI FseI FspI HaeII HapII HgaI Hin1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MaeI MfeI MflI MluI MroI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SpeI SphI Sse8387I SspBI SspI StyI SwaI TaqI Tth111I TthHB8I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 32 enzymes) # ################################### AcsI 331 GAATTC [GA]^AATT[CT] AfaI 177 GTAC GT^AC ApoI 331 GAATTC [GA]^AATT[CT] AvaII 269 GGACC G^G[AT]CC BsaBI 660 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsgI 288 CTGCAC GTGCAG(16/14) BsmAI 494 GTCTC GTCTC(1/5) BspHI 131 TCATGA T^CATGA BspMI 718 ACCTGC ACCTGC(4/8) BsrDI 613 GCAATG GCAATG(2/0) BstXI 236 CCAAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CfoI 390 GCGC GCG^C Cfr13I 269 GGACC G^G[ACGT]CC EcoNI 650 CCTACACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 331 GAATTC G^AATTC FauI 414 GCGGG CCCGC(4/6) HhaI 390 GCGC GCG^C HinP1I 390 GCGC G^CGC MamI 660 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MspA1I 408 CAGCTG C[AC]G^C[GT]G PflMI 437 CCAGCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 492 GAGTC GAGTC(4/5) PvuII 408 CAGCTG CAG^CTG RcaI 131 TCATGA T^CATGA RsaI 177 GTAC GT^AC Sau96I 269 GGACC G^G[ACGT]CC SfcI 867 CTACAG C^T[GA][CT]AG SnaBI 771 TACGTA TAC^GTA StuI 707 AGGCCT AGG^CCT Tsp509I 332 AATT ^AATT Van91I 437 CCAGCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 548 CCATGCTGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AccI 768 GTATAC GT^[AC][GT]AC 774 GTATAC AciI 363 CCGC CCGC(-3/-1) 414 GCGG AlwI 429 GATCC GGATC(4/5) 624 GATCC BanI 540 GGCACC G^G[CT][GA]CC 895 GGTGCC BanII 46 GGGCTC G[GA]GC[CT]^C 346 GGGCTC BbsI 100 GTCTTC GAAGAC(2/6) 817 GTCTTC BbvCI 186 CCTCAGC CCTCAGC(-5/-2) 552 GCTGAGG BpuAI 100 GTCTTC GAAGAC(2/6) 817 GTCTTC BsaAI 367 TACGTG [CT]AC^GT[GA] 771 TACGTA BseRI 184 CTCCTC GAGGAG(10/8) 472 CTCCTC BsmFI 268 GGGAC GGGAC(10/14) 569 GGGAC BssKI 432 CCTGG ^CC[ACGT]GG 656 CCAGG Bst1107I 768 GTATAC GTA^TAC 774 GTATAC BstNI 432 CCTGG CC^[AT]GG 656 CCAGG BstZ17I 768 GTATAC GTA^TAC 774 GTATAC EcoRII 432 CCTGG ^CC[AT]GG 656 CCAGG MaeII 368 ACGT A^CGT 772 ACGT MaeIII 640 GTGAC ^GT[ACGT]AC 742 GTGAC MseI 146 TTAA T^TAA 853 TTAA MvaI 432 CCTGG CC^[AT]GG 656 CCAGG SapI 48 GCTCTTC GCTCTTC(1/4) 722 GCTCTTC ScrFI 432 CCTGG CC^[ACGT]GG 656 CCAGG TfiI 74 GATTC G^A[AT]TC 660 GATTC Tru9I 146 TTAA T^TAA 853 TTAA Tsp45I 640 GTGAC ^GT[GC]AC 742 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 34 enzymes) # ######################################### AluI BalI BbvI BmyI BsiYI BslI Bsp1286I BstF5I Cac8I DdeI DpnII EaeI EarI Fnu4HI FokI HaeIII HinfI HphI ItaI Ksp632I MboI MboII MluNI MnlI MscI MslI MwoI NdeII NlaIII NlaIV Sau3AI SfaNI TseI TspRI