(Circular) (Six-base) MAPSORT of: pFN21AE5390.seq Check: 4587 from: 1 to: 5629 pFN21AE5390 5629 bp With 182 enzymes: SgfI * January 30, 2014 17:18 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2732 2732 Size: 5629 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3439 278 Size: 53 83 186 936 1903 2468 Fragments arranged by size: 2468 1903 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2774 2780 2972 1937 Size: 837 6 192 4594 Fragments arranged by size: 4594 837 192 6 Acc65I G'GTAC_C Cuts at: 2950 2950 Size: 5629 AclI AA'CG_TT Cuts at: 1563 3756 4129 1563 Size: 2193 373 3063 Fragments arranged by size: 3063 2193 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2718 3692 5076 5344 1632 Size: 1086 974 1384 268 1917 Fragments arranged by size: 1917 1384 1086 974 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5432 AflIII A'CryG_T Cuts at: 1203 4528 5505 1203 Size: 3325 977 1327 Fragments arranged by size: 3325 1327 977 AgeI A'CCGG_T Cuts at: 4460 4460 Size: 5629 AhdI GACnn_n'nnGTC Cuts at: 4358 4358 Size: 5629 AleI CACnn'nnGTG Cuts at: 1166 2234 1166 Size: 1068 4561 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2408 1184 Size: 1224 4405 AlwNI CAG_nnn'CTG Cuts at: 3372 4944 3372 Size: 1572 4057 ApaLI G'TGCA_C Cuts at: 3686 4842 3686 Size: 1156 4473 ApoI r'AATT_y Cuts at: 1493 2336 2938 3164 3316 5425 1493 Size: 843 602 226 152 2109 1697 Fragments arranged by size: 2109 1697 843 602 226 152 AseI AT'TA_AT Cuts at: 160 1032 4183 160 Size: 872 3151 1606 Fragments arranged by size: 3151 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5629 AvaI C'yCGr_G Cuts at: 1870 1943 2954 1870 Size: 73 1011 4545 Fragments arranged by size: 4545 1011 73 BamHI G'GATC_C Cuts at: 2959 3326 2959 Size: 367 5262 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2545 2900 2950 4405 618 Size: 325 217 627 758 355 50 1455 1842 Fragments arranged by size: 1842 1455 758 627 355 325 217 50 BanII G_rGCy'C Cuts at: 1391 2055 2355 2948 1391 Size: 664 300 593 4072 Fragments arranged by size: 4072 664 593 300 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2098 2815 928 Size: 971 199 717 3742 Fragments arranged by size: 3742 971 717 199 BbvCI CC'TCA_GC Cuts at: 2192 2558 2192 Size: 366 5263 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3820 3854 1903 Size: 34 1883 34 3678 Fragments arranged by size: 3678 1883 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3521 4731 3521 Size: 1210 4419 BclI T'GATC_A Cuts at: 1548 1548 Size: 5629 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4240 136 Size: 107 122 71 631 605 2568 1525 Fragments arranged by size: 2568 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5624 5624 Size: 5629 BlpI GC'TnA_GC Cuts at: 3046 3046 Size: 5629 Bme1580I G_kGCm'C Cuts at: 1790 2241 3690 4846 1790 Size: 451 1449 1156 2573 Fragments arranged by size: 2573 1449 1156 451 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5629 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3360 4318 442 Size: 416 53 83 399 24 1943 958 1753 Fragments arranged by size: 1943 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5629 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4289 1239 Size: 3050 2579 Bpu10I CC'TnA_GC Cuts at: 2074 2192 2558 2074 Size: 118 366 5145 Fragments arranged by size: 5145 366 118 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3754 4619 4917 3754 Size: 865 298 4466 Fragments arranged by size: 4466 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4292 882 Size: 617 2793 2219 Fragments arranged by size: 2793 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2374 2778 3420 493 Size: 711 1170 404 642 2702 Fragments arranged by size: 2702 1170 711 642 404 BsaBI GATnn'nnATC Cuts at: 2669 5623 2669 Size: 2954 2675 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3436 3818 4453 275 Size: 53 83 186 936 1903 382 635 1451 Fragments arranged by size: 1903 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4062 4460 4734 4881 5292 4062 Size: 398 274 147 411 4399 Fragments arranged by size: 4399 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5404 Fragments arranged by size: 5404 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2180 2468 1186 Size: 994 288 4347 Fragments arranged by size: 4347 994 288 BseYI C'CCAG_C Cuts at: 2347 4832 5328 2347 Size: 2485 496 2648 Fragments arranged by size: 2648 2485 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2278 1163 Size: 573 542 4514 Fragments arranged by size: 4514 573 542 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3079 3840 4868 1540 Size: 275 186 1078 761 1028 2301 Fragments arranged by size: 2301 1078 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3690 3775 4846 3690 Size: 85 1071 4473 Fragments arranged by size: 4473 1071 85 BsmI GAATG_Cn' Cuts at: 3140 3233 3140 Size: 93 5536 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2055 2241 2355 2948 3690 3775 4846 Size: 399 265 186 114 593 742 85 1071 Cuts at: 4846 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 593 399 265 186 114 85 BspEI T'CCGG_A Cuts at: 5292 5292 Size: 5629 BspHI T'CATG_A Cuts at: 2136 3518 5248 2136 Size: 1382 1730 2517 Fragments arranged by size: 2517 1730 1382 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2732 2984 844 Size: 1888 252 3489 Fragments arranged by size: 3489 1888 252 BsrBI CCG'CTC Cuts at: 1394 1823 3516 1394 Size: 429 1693 3507 Fragments arranged by size: 3507 1693 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2625 4124 4298 65 Size: 1183 1377 1499 174 1396 Fragments arranged by size: 1499 1396 1377 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4273 4460 1700 Size: 110 2463 187 2869 Fragments arranged by size: 2869 2463 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5629 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5629 BssSI C'ACGA_G Cuts at: 3689 4701 3689 Size: 1012 4617 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5629 BstBI TT'CG_AA Cuts at: 4433 4433 Size: 5629 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5629 BstXI CCAn_nnnn'nTGG Cuts at: 2248 2248 Size: 5629 BstYI r'GATC_y Cuts at: 1972 2959 3326 3711 3728 5180 5266 5278 5624 Size: 987 367 385 17 1452 86 12 346 Cuts at: 5624 1972 Size: 1977 Fragments arranged by size: 1977 1452 987 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2775 2781 2775 Size: 6 5623 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5077 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2272 487 Size: 683 56 1046 3844 Fragments arranged by size: 3844 1046 683 56 BtsI GCAGTG_nn' Cuts at: 2295 2390 3145 3939 3959 2295 Size: 95 755 794 20 3965 Fragments arranged by size: 3965 794 755 95 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5629 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5594 DraI TTT'AAA Cuts at: 2933 2933 Size: 5629 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4636 809 Size: 507 3320 1802 Fragments arranged by size: 3320 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2115 2376 2439 Size: 54 1425 50 275 18 285 261 63 Cuts at: 2439 3076 3965 5594 8 Size: 637 889 1629 43 Fragments arranged by size: 1629 1425 889 637 285 275 261 63 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3076 1537 Size: 275 1264 4090 Fragments arranged by size: 4090 1264 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2060 2734 2903 3559 1353 Size: 707 674 169 656 3423 Fragments arranged by size: 3423 707 674 656 169 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4216 4590 4736 387 Size: 1064 138 2627 374 146 1280 Fragments arranged by size: 2627 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2718 3692 4289 5076 5344 1239 Size: 393 1086 974 597 787 268 1524 Fragments arranged by size: 1524 1086 974 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2659 2659 Size: 5629 EcoRI G'AATT_C Cuts at: 2336 2938 3316 2336 Size: 602 378 4649 Fragments arranged by size: 4649 602 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2731 2731 Size: 5629 FspI TGC'GCA Cuts at: 4135 4135 Size: 5629 HaeII r_GCGC'y Cuts at: 1678 4776 1678 Size: 3098 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4270 4302 4344 4376 1373 Size: 32 135 32 2698 32 42 32 2626 Fragments arranged by size: 2698 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2973 3219 669 Size: 988 281 1035 246 3079 Fragments arranged by size: 3079 1035 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5629 HpaI GTT'AAC Cuts at: 3219 3219 Size: 5629 KpnI G_GTAC'C Cuts at: 2954 2954 Size: 5629 MfeI C'AATT_G Cuts at: 3228 3228 Size: 5629 MluI A'CGCG_T Cuts at: 5505 5505 Size: 5629 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4743 4927 1305 Size: 3438 184 2007 Fragments arranged by size: 3438 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2117 2378 2441 10 Size: 54 1425 343 285 261 63 3198 Fragments arranged by size: 3198 1425 343 285 261 63 54 MslI CAynn'nnrTG Cuts at: 518 1166 2117 2177 2234 2492 2675 2741 3587 Size: 648 951 60 57 258 183 66 846 Cuts at: 3587 3946 4105 518 Size: 359 159 2042 Fragments arranged by size: 2042 951 846 648 359 258 183 159 66 60 57 MspA1I CmG'CkG Cuts at: 1606 2415 3045 3722 4870 5115 1606 Size: 809 630 677 1148 245 2120 Fragments arranged by size: 2120 1148 809 677 630 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5077 NdeI CA'TA_TG Cuts at: 387 387 Size: 5629 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5629 NotI GC'GGCC_GC Cuts at: 3076 3076 Size: 5629 NspI r_CATG'y Cuts at: 1132 1229 2987 4532 1132 Size: 97 1758 1545 2229 Fragments arranged by size: 2229 1758 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5629 PciI A'CATG_T Cuts at: 4528 4528 Size: 5629 PflMI CCAn_nnn'nTGG Cuts at: 1108 2448 1108 Size: 1340 4289 PmeI GTTT'AAAC Cuts at: 2933 2933 Size: 5629 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3698 3730 5211 5243 3698 Size: 32 1481 32 4084 Fragments arranged by size: 4084 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5629 PsiI TTA'TAA Cuts at: 3199 3199 Size: 5629 PstI C_TGCA'G Cuts at: 830 2981 830 Size: 2151 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5629 PvuII CAG'CTG Cuts at: 2415 2415 Size: 5629 SalI G'TCGA_C Cuts at: 1936 2971 1936 Size: 1035 4594 SapI GCTCTTCn'nnn_ Cuts at: 2060 2734 2060 Size: 674 4955 SbfI CC_TGCA'GG Cuts at: 2981 2981 Size: 5629 ScaI AGT'ACT Cuts at: 3877 5589 3877 Size: 1712 3917 SfcI C'TryA_G Cuts at: 826 1045 2872 2977 4112 4793 4984 826 Size: 219 1827 105 1135 681 191 1471 Fragments arranged by size: 1827 1471 1135 681 219 191 105 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5629 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5629 SmaI CCC'GGG Cuts at: 1872 2956 1872 Size: 1084 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3733 4634 4896 820 Size: 197 926 1790 901 262 1553 Fragments arranged by size: 1790 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2778 3420 493 Size: 2285 642 2702 Fragments arranged by size: 2702 2285 642 SpeI A'CTAG_T Cuts at: 152 152 Size: 5629 SphI G_CATG'C Cuts at: 1132 2987 1132 Size: 1855 3774 SspI AAT'ATT Cuts at: 5 52 3553 5 Size: 47 3501 2081 Fragments arranged by size: 3501 2081 47 StuI AGG'CCT Cuts at: 1517 2714 1517 Size: 1197 4432 StyI C'CwwG_G Cuts at: 513 1065 3068 513 Size: 552 2003 3074 Fragments arranged by size: 3074 2003 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2563 2888 3656 3673 3826 4011 4456 Size: 1255 983 325 768 17 153 185 445 Cuts at: 4456 325 Size: 1498 Fragments arranged by size: 1498 1255 983 768 445 325 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2181 3875 5587 Size: 274 80 33 51 1443 204 1694 1712 Cuts at: 5587 96 Size: 138 Fragments arranged by size: 1712 1694 1443 274 204 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5589 5621 5589 Size: 32 5597 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5629 XbaI T'CTAG_A Cuts at: 2965 2965 Size: 5629 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2560 2560 Size: 5629 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5629 XmaI C'CCGG_G Cuts at: 1870 2954 1870 Size: 1084 4545 XmnI GAAnn'nnTTC Cuts at: 3758 3758 Size: 5629 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3437 276 Size: 53 83 186 936 1903 2468 Fragments arranged by size: 2468 1903 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI Bsu36I DraIII EcoICRI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI