################################## # NO CUT ENZYMES ( 166 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AcyI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MaeI MamI MfeI MluI MroI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI PvuI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI SspI StyI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AclI 508 AACGTT AA^CGTT AlwNI 802 CAGGATCTG CAG[ACGT][ACGT][ACGT]^CTG AspHI 667 GTGCTC G[AT]GC[AT]^C AvaI 670 CTCGGG C^[CT]CG[GA]G AvaII 503 GGTCC G^G[AT]CC BalI 890 TGGCCA TGG^CCA BmyI 667 GTGCTC G[AGT]GC[ACT]^C BsaJI 800 CCCAGG C^C[ACGT][ACGT]GG BsiEI 662 CGACCG CG[GA][CT]^CG BsiHKAI 667 GTGCTC G[AT]GC[AT]^C BsmAI 595 GTCTC GTCTC(1/5) BsmFI 226 GTCCC GGGAC(10/14) BsoBI 670 CTCGGG C^[CT]CG[GA]G Bsp1286I 667 GTGCTC G[AGT]GC[ACT]^C BsrDI 575 GCAATG GCAATG(2/0) BssSI 301 CTCGTG CACGAG(-5/-1) BstXI 14 CCACCGTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 804 GGATCT [GA]^GATC[CT] Cfr13I 503 GGTCC G^G[ACGT]CC EaeI 890 TGGCCA [CT]^GGCC[GA] FauI 277 GCGGG CCCGC(4/6) MaeII 509 ACGT A^CGT MflI 804 GGATCT [GA]^GATC[CT] MluNI 890 TGGCCA TGG^CCA MscI 890 TGGCCA TGG^CCA MseI 848 TTAA T^TAA MspA1I 487 CAGCTG C[AC]G^C[GT]G MwoI 784 GCGATACCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC Psp1406I 508 AACGTT AA^CGTT PstI 791 CTGCAG CTGCA^G PvuII 487 CAGCTG CAG^CTG SapI 758 GCTCTTC GCTCTTC(1/4) Sau96I 503 GGTCC G^G[ACGT]CC SphI 464 GCATGC GCATG^C StuI 701 AGGCCT AGG^CCT Tru9I 848 TTAA T^TAA XhoII 804 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AcsI 25 GAATTT [GA]^AATT[CT] 914 GAATTT AfaI 171 GTAC GT^AC 768 GTAC AlwI 810 GATCC GGATC(4/5) 804 GGATC AocI 180 CCTCAGG CC^T[ACGT]AGG 771 CCTCAGG ApoI 25 GAATTT [GA]^AATT[CT] 914 GAATTT BbsI 295 GTCTTC GAAGAC(2/6) 742 GTCTTC BpuAI 295 GTCTTC GAAGAC(2/6) 742 GTCTTC BseRI 31 CTCCTC GAGGAG(10/8) 683 GAGGAG BssKI 729 CCTGG ^CC[ACGT]GG 801 CCAGG BstNI 729 CCTGG CC^[AT]GG 801 CCAGG Bsu36I 180 CCTCAGG CC^T[ACGT]AGG 771 CCTCAGG EarI 759 CTCTTC CTCTTC(1/4) 909 GAAGAG Eco81I 180 CCTCAGG CC^T[ACGT]AGG 771 CCTCAGG EcoRII 729 CCTGG ^CC[AT]GG 801 CCAGG HinfI 186 GAATC G^A[ACGT]TC 410 GAATC HphI 137 TCACC GGTGA(8/7) 338 TCACC Ksp632I 759 CTCTTC CTCTTC(1/4) 909 GAAGAG MvaI 729 CCTGG CC^[AT]GG 801 CCAGG NspI 161 ACATGC [GA]CATG^[CT] 464 GCATGC RsaI 171 GTAC GT^AC 768 GTAC ScrFI 729 CCTGG CC^[ACGT]GG 801 CCAGG SfcI 61 CTACAG C^T[GA][CT]AG 791 CTGCAG TaqI 408 TCGA T^CGA 661 TCGA TfiI 186 GAATC G^A[AT]TC 410 GAATC TthHB8I 408 TCGA T^CGA 661 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 30 enzymes) # ######################################### AciI AluI BbvI BsiYI BslI BspHI BspMI BsrI BstF5I Cac8I DdeI DpnII Fnu4HI FokI HaeIII ItaI MaeIII MboI MboII MnlI MslI NdeII NlaIII RcaI Sau3AI SfaNI TseI Tsp45I Tsp509I TspRI