(Circular) (Six-base) MAPSORT of: pFN21AE2520.seq Check: 5886 from: 1 to: 6478 pFN21AE2520 6478 bp With 182 enzymes: SgfI * March 20, 2014 17:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4288 278 Size: 53 83 186 936 2752 2468 Fragments arranged by size: 2752 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3821 1937 Size: 1884 4594 Acc65I G'GTAC_C Cuts at: 3799 3799 Size: 6478 AclI AA'CG_TT Cuts at: 1563 4605 4978 1563 Size: 3042 373 3063 Fragments arranged by size: 3063 3042 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2967 3698 4541 5925 6193 1632 Size: 1335 731 843 1384 268 1917 Fragments arranged by size: 1917 1384 1335 843 731 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6281 AflIII A'CryG_T Cuts at: 1203 5377 6354 1203 Size: 4174 977 1327 Fragments arranged by size: 4174 1327 977 AgeI A'CCGG_T Cuts at: 5309 5309 Size: 6478 AhdI GACnn_n'nnGTC Cuts at: 3583 5207 3583 Size: 1624 4854 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6478 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6478 AlwNI CAG_nnn'CTG Cuts at: 2972 3440 3757 4221 5793 2972 Size: 468 317 464 1572 3657 Fragments arranged by size: 3657 1572 468 464 317 ApaI G_GGCC'C Cuts at: 3012 3012 Size: 6478 ApaLI G'TGCA_C Cuts at: 4535 5691 4535 Size: 1156 5322 ApoI r'AATT_y Cuts at: 1493 2338 2427 3787 4013 4165 6274 1493 Size: 845 89 1360 226 152 2109 1697 Fragments arranged by size: 2109 1697 1360 845 226 152 89 AseI AT'TA_AT Cuts at: 160 1032 5032 160 Size: 872 4000 1606 Fragments arranged by size: 4000 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6478 AvaI C'yCGr_G Cuts at: 1870 1943 3803 1870 Size: 73 1860 4545 Fragments arranged by size: 4545 1860 73 BamHI G'GATC_C Cuts at: 3808 4175 3808 Size: 367 6111 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2074 3799 5254 618 Size: 325 217 627 287 1725 1455 1842 Fragments arranged by size: 1842 1725 1455 627 325 287 217 BanII G_rGCy'C Cuts at: 1391 3012 3735 3797 1391 Size: 1621 723 62 4072 Fragments arranged by size: 4072 1621 723 62 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2293 2443 3162 928 Size: 971 394 150 719 4244 Fragments arranged by size: 4244 971 719 394 150 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4669 4703 1903 Size: 34 2732 34 3678 Fragments arranged by size: 3678 2732 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3223 4370 5580 3223 Size: 1147 1210 4121 Fragments arranged by size: 4121 1210 1147 BclI T'GATC_A Cuts at: 1548 1548 Size: 6478 BfrBI ATG'CAT Cuts at: 2320 2320 Size: 6478 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3359 5089 136 Size: 107 122 71 631 605 1687 1730 1525 Fragments arranged by size: 1730 1687 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6473 6473 Size: 6478 BlpI GC'TnA_GC Cuts at: 3895 3895 Size: 6478 Bme1580I G_kGCm'C Cuts at: 1790 3012 3355 4539 5695 1790 Size: 1222 343 1184 1156 2573 Fragments arranged by size: 2573 1222 1184 1156 343 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6478 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3627 4209 5167 Size: 416 53 83 399 24 2210 582 958 Cuts at: 5167 442 Size: 1753 Fragments arranged by size: 2210 1753 958 582 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6478 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3250 3250 Size: 6478 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2492 2507 2567 2597 2636 2654 2672 2729 Size: 1253 15 60 30 39 18 18 57 Cuts at: 2729 3523 5138 1239 Size: 794 1615 2579 Fragments arranged by size: 2579 1615 1253 794 60 57 39 30 18 18 15 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2376 4603 5468 5766 2376 Size: 2227 865 298 3088 Fragments arranged by size: 3088 2227 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2273 5141 882 Size: 617 774 2868 2219 Fragments arranged by size: 2868 2219 774 617 BsaAI yAC'GTr Cuts at: 493 1204 4269 493 Size: 711 3065 2702 Fragments arranged by size: 3065 2702 711 BsaBI GATnn'nnATC Cuts at: 6472 6472 Size: 6478 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4285 4667 5302 275 Size: 53 83 186 936 2752 382 635 1451 Fragments arranged by size: 2752 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4911 5309 5583 5730 6141 4911 Size: 398 274 147 411 5248 Fragments arranged by size: 5248 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3028 3058 1178 Size: 30 165 30 1625 30 4598 Fragments arranged by size: 4598 1625 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2820 2940 2988 3012 3048 3057 3081 3084 Size: 1634 120 48 24 36 9 24 3 Cuts at: 3084 3382 1186 Size: 298 4282 Fragments arranged by size: 4282 1634 298 120 48 36 24 24 9 3 BseYI C'CCAG_C Cuts at: 2845 5681 6177 2845 Size: 2836 496 3146 Fragments arranged by size: 3146 2836 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2958 1163 Size: 573 1222 4683 Fragments arranged by size: 4683 1222 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2898 3928 4689 5717 1540 Size: 275 186 897 1030 761 1028 2301 Fragments arranged by size: 2301 1030 1028 897 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3735 4539 4624 5695 3735 Size: 804 85 1071 4518 Fragments arranged by size: 4518 1071 804 85 BsmI GAATG_Cn' Cuts at: 3259 3989 4082 3259 Size: 730 93 5655 Fragments arranged by size: 5655 730 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3012 3355 3735 3797 4539 4624 5695 Size: 399 1222 343 380 62 742 85 1071 Cuts at: 5695 1391 Size: 2174 Fragments arranged by size: 2174 1222 1071 742 399 380 343 85 62 BspEI T'CCGG_A Cuts at: 6141 6141 Size: 6478 BspHI T'CATG_A Cuts at: 4367 6097 4367 Size: 1730 4748 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2395 3833 844 Size: 1551 1438 3489 Fragments arranged by size: 3489 1551 1438 BsrBI CCG'CTC Cuts at: 1394 1823 2476 2620 2638 2656 4365 1394 Size: 429 653 144 18 18 1709 3507 Fragments arranged by size: 3507 1709 653 429 144 18 18 BsrDI GCAATG_nn' Cuts at: 65 1248 2360 4973 5147 65 Size: 1183 1112 2613 174 1396 Fragments arranged by size: 2613 1396 1183 1112 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2893 5122 5309 1700 Size: 110 1083 2229 187 2869 Fragments arranged by size: 2869 2229 1083 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6478 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6478 BssSI C'ACGA_G Cuts at: 4538 5550 4538 Size: 1012 5466 BstAPI GCAn_nnn'nTGC Cuts at: 794 2036 794 Size: 1242 5236 BstBI TT'CG_AA Cuts at: 5282 5282 Size: 6478 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6478 BstYI r'GATC_y Cuts at: 1972 3808 4175 4560 4577 6029 6115 6127 6473 Size: 1836 367 385 17 1452 86 12 346 Cuts at: 6473 1972 Size: 1977 Fragments arranged by size: 1977 1836 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3298 513 Size: 552 2233 3693 Fragments arranged by size: 3693 2233 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5739 Fragments arranged by size: 5739 683 56 BtsI GCAGTG_nn' Cuts at: 3994 4788 4808 3994 Size: 794 20 5664 Fragments arranged by size: 5664 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6478 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6443 DraI TTT'AAA Cuts at: 3782 3782 Size: 6478 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2174 5485 809 Size: 507 858 3311 1802 Fragments arranged by size: 3311 1802 858 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2895 3360 3925 Size: 54 1425 50 275 18 1065 465 565 Cuts at: 3925 4814 6443 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1065 889 565 465 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2895 3925 1537 Size: 275 1083 1030 4090 Fragments arranged by size: 4090 1083 1030 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2860 3463 3723 4408 1353 Size: 1507 603 260 685 3423 Fragments arranged by size: 3423 1507 685 603 260 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3262 5065 5439 5585 387 Size: 1064 138 1673 1803 374 146 1280 Fragments arranged by size: 1803 1673 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3733 3733 Size: 6478 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2492 2507 2567 2597 2636 2654 2672 Size: 393 860 15 60 30 39 18 18 Cuts at: 2672 2729 2967 3523 3698 4541 5138 5925 6193 Size: 57 238 556 175 843 597 787 268 Cuts at: 6193 1239 Size: 1524 Fragments arranged by size: 1524 860 843 787 597 556 393 268 238 175 60 57 39 30 18 18 15 EcoNI CCTnn'n_nnAGG Cuts at: 3003 3003 Size: 6478 EcoO109I rG'GnC_Cy Cuts at: 2969 3008 3009 3585 2969 Size: 39 1 576 5862 Fragments arranged by size: 5862 576 39 1 EcoRI G'AATT_C Cuts at: 3787 4165 3787 Size: 378 6100 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3507 3507 Size: 6478 FspI TGC'GCA Cuts at: 4984 4984 Size: 6478 HaeII r_GCGC'y Cuts at: 1678 5625 1678 Size: 3947 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2008 2040 2097 2129 2891 Size: 32 135 32 436 32 57 32 762 Cuts at: 2891 2923 3569 3601 5119 5151 5193 5225 1373 Size: 32 646 32 1518 32 42 32 2626 Fragments arranged by size: 2626 1518 762 646 436 135 57 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3822 4068 669 Size: 988 281 1884 246 3079 Fragments arranged by size: 3079 1884 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6478 HpaI GTT'AAC Cuts at: 4068 4068 Size: 6478 KpnI G_GTAC'C Cuts at: 3803 3803 Size: 6478 MfeI C'AATT_G Cuts at: 3189 4077 3189 Size: 888 5590 MluI A'CGCG_T Cuts at: 6354 6354 Size: 6478 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2496 5592 5776 1305 Size: 1191 3096 184 2007 Fragments arranged by size: 3096 2007 1191 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3362 10 Size: 54 1425 343 1530 3126 Fragments arranged by size: 3126 1530 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4436 4795 4954 518 Size: 648 3270 359 159 2042 Fragments arranged by size: 3270 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2081 2575 2770 3185 3894 4571 5719 5964 Size: 475 494 195 415 709 677 1148 245 Cuts at: 5964 1606 Size: 2120 Fragments arranged by size: 2120 1148 709 677 494 475 415 245 195 NaeI GCC'GGC Cuts at: 2895 2895 Size: 6478 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5926 NdeI CA'TA_TG Cuts at: 387 387 Size: 6478 NgoMIV G'CCGG_C Cuts at: 2893 2893 Size: 6478 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6478 NotI GC'GGCC_GC Cuts at: 3925 3925 Size: 6478 NsiI A_TGCA'T Cuts at: 2322 2322 Size: 6478 NspI r_CATG'y Cuts at: 1132 1229 2324 3836 5381 1132 Size: 97 1095 1512 1545 2229 Fragments arranged by size: 2229 1545 1512 1095 97 PasI CC'CwG_GG Cuts at: 1348 3005 1348 Size: 1657 4821 PciI A'CATG_T Cuts at: 5377 5377 Size: 6478 PflMI CCAn_nnn'nTGG Cuts at: 1108 2084 1108 Size: 976 5502 PmeI GTTT'AAAC Cuts at: 3782 3782 Size: 6478 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3260 3292 4547 4579 6060 6092 3260 Size: 32 1255 32 1481 32 3646 Fragments arranged by size: 3646 1481 1255 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3585 3585 Size: 6478 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6478 PsiI TTA'TAA Cuts at: 4048 4048 Size: 6478 PspOMI G'GGCC_C Cuts at: 3008 3008 Size: 6478 PstI C_TGCA'G Cuts at: 830 3778 3830 830 Size: 2948 52 3478 Fragments arranged by size: 3478 2948 52 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6478 PvuII CAG'CTG Cuts at: 2081 2575 2770 3185 2081 Size: 494 195 415 5374 Fragments arranged by size: 5374 494 415 195 SacI G_AGCT'C Cuts at: 3735 3735 Size: 6478 SalI G'TCGA_C Cuts at: 1936 3820 1936 Size: 1884 4594 SapI GCTCTTCn'nnn_ Cuts at: 3723 3723 Size: 6478 SbfI CC_TGCA'GG Cuts at: 3830 3830 Size: 6478 ScaI AGT'ACT Cuts at: 4726 6438 4726 Size: 1712 4766 SfcI C'TryA_G Cuts at: 826 1045 3774 3826 4961 5642 5833 826 Size: 219 2729 52 1135 681 191 1471 Fragments arranged by size: 2729 1471 1135 681 219 191 52 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6478 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6478 SmaI CCC'GGG Cuts at: 1872 3805 1872 Size: 1933 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2391 4582 5483 5745 820 Size: 197 926 448 2191 901 262 1553 Fragments arranged by size: 2191 1553 926 901 448 262 197 SnaBI TAC'GTA Cuts at: 493 4269 493 Size: 3776 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6478 SphI G_CATG'C Cuts at: 1132 2324 3836 1132 Size: 1192 1512 3774 Fragments arranged by size: 3774 1512 1192 SspI AAT'ATT Cuts at: 5 52 4402 5 Size: 47 4350 2081 Fragments arranged by size: 4350 2081 47 StuI AGG'CCT Cuts at: 1517 3455 1517 Size: 1938 4540 StyI C'CwwG_G Cuts at: 513 1065 3491 3554 3917 513 Size: 552 2426 63 363 3074 Fragments arranged by size: 3074 2426 552 363 63 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2062 4505 4522 4675 4860 5305 325 Size: 1255 482 2443 17 153 185 445 1498 Fragments arranged by size: 2443 1498 1255 482 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2211 3467 4724 Size: 274 80 33 51 1443 234 1256 1257 Cuts at: 4724 6436 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1257 1256 274 234 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2217 2249 3028 3060 6438 6470 2217 Size: 32 779 32 3378 32 2225 Fragments arranged by size: 3378 2225 779 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6478 XbaI T'CTAG_A Cuts at: 3814 3814 Size: 6478 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6478 XmaI C'CCGG_G Cuts at: 1870 3803 1870 Size: 1933 4545 XmnI GAAnn'nnTTC Cuts at: 4607 4607 Size: 6478 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4286 276 Size: 53 83 186 936 2752 2468 Fragments arranged by size: 2752 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI NarI NruI PacI PfoI PmlI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI