(Linear) (Six-base) MAPSORT of: KIEE2512.seq Check: 9830 from: 1 to: 864 KIEE2512.orf With 182 enzymes: SgfI * February 25, 2014 15:45 .. AatII G_ACGT'C Cuts at: 0 390 864 Size: 390 474 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 116 185 479 864 Size: 116 69 294 385 Fragments arranged by size: 385 294 116 69 AlwNI CAG_nnn'CTG Cuts at: 0 106 312 864 Size: 106 206 552 Fragments arranged by size: 552 206 106 BanI G'GyrC_C Cuts at: 0 222 864 Size: 222 642 BanII G_rGCy'C Cuts at: 0 359 471 864 Size: 359 112 393 Fragments arranged by size: 393 359 112 BbvCI CC'TCA_GC Cuts at: 0 503 685 864 Size: 503 182 179 Fragments arranged by size: 503 182 179 BfrBI ATG'CAT Cuts at: 0 546 864 Size: 546 318 Bpu10I CC'TnA_GC Cuts at: 0 503 685 864 Size: 503 182 179 Fragments arranged by size: 503 182 179 BsaI GGTCTCn'nnnn_ Cuts at: 0 103 112 864 Size: 103 9 752 Fragments arranged by size: 752 103 9 BsaHI Gr'CG_yC Cuts at: 0 387 864 Size: 387 477 BsaWI w'CCGG_w Cuts at: 0 272 419 864 Size: 272 147 445 Fragments arranged by size: 445 272 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 236 266 864 Size: 236 30 598 Fragments arranged by size: 598 236 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 49 52 55 88 130 196 361 364 Size: 49 3 3 33 42 66 165 3 Cuts at: 364 367 493 496 499 657 864 Size: 3 126 3 3 158 207 Fragments arranged by size: 207 165 158 126 66 49 42 33 3 3 3 3 3 3 BsiHKAI G_wGCw'C Cuts at: 0 359 864 Size: 359 505 BsmBI CGTCTCn'nnnn_ Cuts at: 0 378 864 Size: 378 486 Bsp1286I G_dGCh'C Cuts at: 0 359 471 864 Size: 359 112 393 Fragments arranged by size: 393 359 112 BspEI T'CCGG_A Cuts at: 0 419 864 Size: 419 445 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 755 864 Size: 755 109 BsrBI CCG'CTC Cuts at: 0 399 429 441 585 612 864 Size: 399 30 12 144 27 252 Fragments arranged by size: 399 252 144 30 27 12 BstYI r'GATC_y Cuts at: 0 233 864 Size: 233 631 BtgI C'CryG_G Cuts at: 0 831 864 Size: 831 33 EaeI y'GGCC_r Cuts at: 0 656 864 Size: 656 208 EarI CTCTTCn'nnn_ Cuts at: 0 8 20 59 80 140 455 592 864 Size: 8 12 39 21 60 315 137 272 Fragments arranged by size: 315 272 137 60 39 21 12 8 EcoICRI GAG'CTC Cuts at: 0 357 864 Size: 357 507 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 116 185 479 864 Size: 116 69 294 385 Fragments arranged by size: 385 294 116 69 EcoO109I rG'GnC_Cy Cuts at: 0 291 864 Size: 291 573 HaeII r_GCGC'y Cuts at: 0 313 858 864 Size: 313 545 6 Fragments arranged by size: 545 313 6 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 306 338 864 Size: 306 32 526 Fragments arranged by size: 526 306 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 560 864 Size: 560 304 MscI TGG'CCA Cuts at: 0 658 864 Size: 658 206 MslI CAynn'nnrTG Cuts at: 0 671 864 Size: 671 193 MspA1I CmG'CkG Cuts at: 0 58 82 684 833 864 Size: 58 24 602 149 31 Fragments arranged by size: 602 149 58 31 24 NsiI A_TGCA'T Cuts at: 0 548 864 Size: 548 316 PpuMI rG'GwC_Cy Cuts at: 0 291 864 Size: 291 573 PstI C_TGCA'G Cuts at: 0 752 864 Size: 752 112 PvuII CAG'CTG Cuts at: 0 58 684 864 Size: 58 626 180 Fragments arranged by size: 626 180 58 SacI G_AGCT'C Cuts at: 0 359 864 Size: 359 505 SacII CC_GC'GG Cuts at: 0 834 864 Size: 834 30 SapI GCTCTTCn'nnn_ Cuts at: 0 59 864 Size: 59 805 SbfI CC_TGCA'GG Cuts at: 0 752 864 Size: 752 112 SfcI C'TryA_G Cuts at: 0 748 864 Size: 748 116 StyI C'CwwG_G Cuts at: 0 698 864 Size: 698 166 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 127 864 Size: 127 737 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 236 268 864 Size: 236 32 596 Fragments arranged by size: 596 236 32 ZraI GAC'GTC Cuts at: 0 388 864 Size: 388 476 Enzymes that do cut: AatII AcuI AlwNI BanI BanII BbvCI BfrBI Bpu10I BsaI BsaHI BsaWI BsaXI BseRI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI BstYI BtgI EaeI EarI EcoICRI Eco57MI EcoO109I HaeII Hin4I MmeI MscI MslI MspA1I NsiI PpuMI PstI PvuII SacI SacII SapI SbfI SfcI StyI TaqII TstI ZraI Enzymes that do not cut: AarI AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaAI BsaBI BseYI BsgI BsiEI BsiWI BsmI BspHI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI EagI EciI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SalI SanDI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TatI Tth111I XbaI XcmI XhoI XmaI XmnI