Restriction map for pFN21AE2512
 (Linear) (Six-base) MAPSORT of: KIEE2512.seq  Check: 9830  from: 1  to: 864



KIEE2512.orf

 With 182 enzymes: SgfI * 

                        February 25, 2014 15:45  ..

AatII G_ACGT'C

Cuts at:      0     390     864 
   Size:        390     474
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     116     185     479     864 
   Size:        116      69     294     385

  Fragments arranged by size: 

                 385     294     116      69
 
AlwNI CAG_nnn'CTG

Cuts at:      0     106     312     864 
   Size:        106     206     552

  Fragments arranged by size: 

                 552     206     106
 
BanI G'GyrC_C

Cuts at:      0     222     864 
   Size:        222     642
 
BanII G_rGCy'C

Cuts at:      0     359     471     864 
   Size:        359     112     393

  Fragments arranged by size: 

                 393     359     112
 
BbvCI CC'TCA_GC

Cuts at:      0     503     685     864 
   Size:        503     182     179

  Fragments arranged by size: 

                 503     182     179
 
BfrBI ATG'CAT

Cuts at:      0     546     864 
   Size:        546     318
 
Bpu10I CC'TnA_GC

Cuts at:      0     503     685     864 
   Size:        503     182     179

  Fragments arranged by size: 

                 503     182     179
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     103     112     864 
   Size:        103       9     752

  Fragments arranged by size: 

                 752     103       9
 
BsaHI Gr'CG_yC

Cuts at:      0     387     864 
   Size:        387     477
 
BsaWI w'CCGG_w

Cuts at:      0     272     419     864 
   Size:        272     147     445

  Fragments arranged by size: 

                 445     272     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     236     266     864 
   Size:        236      30     598

  Fragments arranged by size: 

                 598     236      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      49      52      55      88     130     196     361     364 
   Size:         49       3       3      33      42      66     165       3

Cuts at:    364     367     493     496     499     657     864 
   Size:          3     126       3       3     158     207

  Fragments arranged by size: 

                 207     165     158     126      66      49      42      33
                   3       3       3       3       3       3
 
BsiHKAI G_wGCw'C

Cuts at:      0     359     864 
   Size:        359     505
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     378     864 
   Size:        378     486
 
Bsp1286I G_dGCh'C

Cuts at:      0     359     471     864 
   Size:        359     112     393

  Fragments arranged by size: 

                 393     359     112
 
BspEI T'CCGG_A

Cuts at:      0     419     864 
   Size:        419     445
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     755     864 
   Size:        755     109
 
BsrBI CCG'CTC

Cuts at:      0     399     429     441     585     612     864 
   Size:        399      30      12     144      27     252

  Fragments arranged by size: 

                 399     252     144      30      27      12
 
BstYI r'GATC_y

Cuts at:      0     233     864 
   Size:        233     631
 
BtgI C'CryG_G

Cuts at:      0     831     864 
   Size:        831      33
 
EaeI y'GGCC_r

Cuts at:      0     656     864 
   Size:        656     208
 
EarI CTCTTCn'nnn_

Cuts at:      0       8      20      59      80     140     455     592     864 
   Size:          8      12      39      21      60     315     137     272

  Fragments arranged by size: 

                 315     272     137      60      39      21      12       8
            
 
EcoICRI GAG'CTC

Cuts at:      0     357     864 
   Size:        357     507
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     116     185     479     864 
   Size:        116      69     294     385

  Fragments arranged by size: 

                 385     294     116      69
 
EcoO109I rG'GnC_Cy

Cuts at:      0     291     864 
   Size:        291     573
 
HaeII r_GCGC'y

Cuts at:      0     313     858     864 
   Size:        313     545       6

  Fragments arranged by size: 

                 545     313       6
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     306     338     864 
   Size:        306      32     526

  Fragments arranged by size: 

                 526     306      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     560     864 
   Size:        560     304
 
MscI TGG'CCA

Cuts at:      0     658     864 
   Size:        658     206
 
MslI CAynn'nnrTG

Cuts at:      0     671     864 
   Size:        671     193
 
MspA1I CmG'CkG

Cuts at:      0      58      82     684     833     864 
   Size:         58      24     602     149      31

  Fragments arranged by size: 

                 602     149      58      31      24
 
NsiI A_TGCA'T

Cuts at:      0     548     864 
   Size:        548     316
 
PpuMI rG'GwC_Cy

Cuts at:      0     291     864 
   Size:        291     573
 
PstI C_TGCA'G

Cuts at:      0     752     864 
   Size:        752     112
 
PvuII CAG'CTG

Cuts at:      0      58     684     864 
   Size:         58     626     180

  Fragments arranged by size: 

                 626     180      58
 
SacI G_AGCT'C

Cuts at:      0     359     864 
   Size:        359     505
 
SacII CC_GC'GG

Cuts at:      0     834     864 
   Size:        834      30
 
SapI GCTCTTCn'nnn_

Cuts at:      0      59     864 
   Size:         59     805
 
SbfI CC_TGCA'GG

Cuts at:      0     752     864 
   Size:        752     112
 
SfcI C'TryA_G

Cuts at:      0     748     864 
   Size:        748     116
 
StyI C'CwwG_G

Cuts at:      0     698     864 
   Size:        698     166
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     127     864 
   Size:        127     737
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     236     268     864 
   Size:        236      32     596

  Fragments arranged by size: 

                 596     236      32
 
ZraI GAC'GTC

Cuts at:      0     388     864 
   Size:        388     476
 

 Enzymes that do cut:

    AatII     AcuI    AlwNI     BanI    BanII    BbvCI    BfrBI   Bpu10I
     BsaI    BsaHI    BsaWI    BsaXI    BseRI  BsiHKAI    BsmBI Bsp1286I
    BspEI    BspMI    BsrBI    BstYI     BtgI     EaeI     EarI  EcoICRI
  Eco57MI EcoO109I    HaeII    Hin4I     MmeI     MscI     MslI   MspA1I
     NsiI    PpuMI     PstI    PvuII     SacI    SacII     SapI     SbfI
     SfcI     StyI    TaqII     TstI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI     BbsI
     BcgI    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BplI     BpmI    BpuEI    BsaAI    BsaBI    BseYI
     BsgI    BsiEI    BsiWI     BsmI    BspHI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EagI
     EciI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NspI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SalI
    SanDI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
     TatI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
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