################################## # NO CUT ENZYMES ( 148 enzymes) # ################################## Acc65I AccI AclI AcsI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BamHI BbeI BbrPI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsgI BsiEI BsiWI BsmFI BsmI BsoBI Bsp1407I BspDI BspHI BspLU11I BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV FbaI FseI FspI HgaI HincII HindII HindIII HpaI KasI KpnI MaeI MamI MfeI MluI MseI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI SspBI SspI StuI SwaI Tru9I Tsp509I TspRI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AatII 386 GACGTC GACGT^C AccII 823 CGCG CG^CG AccIII 419 TCCGGA T^CCGGA AcyI 386 GACGTC G[GA]^CG[CT]C AfaI 30 GTAC GT^AC AspHI 355 GAGCTC G[AT]GC[AT]^C AvaII 291 GGACC G^G[AT]CC BalI 647 TGGCCA TGG^CCA BanI 222 GGTGCC G^G[CT][GA]CC BbsI 567 GAAGAC GAAGAC(2/6) BpuAI 567 GAAGAC GAAGAC(2/6) BsaHI 386 GACGTC G[GA]^CG[CT]C BseAI 419 TCCGGA T^CCGGA BsiHKAI 355 GAGCTC G[AT]GC[AT]^C BsmBI 384 GAGACG CGTCTC(1/5) BspEI 419 TCCGGA T^CCGGA BspMI 737 ACCTGC ACCTGC(4/8) BstUI 823 CGCG CG^CG BstYI 233 AGATCC [GA]^GATC[CT] DraII 290 AGGACCT [GA]G^G[ACGT]CC[CT] DsaI 822 CCGCGG C^C[GA][CT]GG EaeI 647 TGGCCA [CT]^GGCC[GA] EcoO109I 290 AGGACCT [GA]G^G[ACGT]CC[CT] EcoT14I 689 CCAAGG C^C[AT][AT]GG EcoT22I 544 ATGCAT ATGCA^T Hin1I 386 GACGTC G[GA]^CG[CT]C HphI 839 TCACC GGTGA(8/7) KspI 822 CCGCGG CCGC^GG MaeII 387 ACGT A^CGT MflI 233 AGATCC [GA]^GATC[CT] MluNI 647 TGGCCA TGG^CCA MroI 419 TCCGGA T^CCGGA MscI 647 TGGCCA TGG^CCA MslI 658 CACCTGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 823 CGCG CG^CG NlaIV 222 GGTGCC GG[ACGT]^[ACGT]CC NsiI 544 ATGCAT ATGCA^T PleI 184 GAGTC GAGTC(4/5) PpuMI 290 AGGACCT [GA]G^G[AT]CC[CT] PstI 739 CTGCAG CTGCA^G RsaI 30 GTAC GT^AC SacI 355 GAGCTC GAGCT^C SacII 822 CCGCGG CCGC^GG SapI 64 GAAGAGC GCTCTTC(1/4) SbfI 738 CCTGCAGG CCTGCA^GG SfcI 739 CTGCAG C^T[GA][CT]AG Sse8387I 738 CCTGCAGG CCTGCA^GG StyI 689 CCAAGG C^C[AT][AT]GG TfiI 621 GATTC G^A[AT]TC Tsp45I 838 GTCAC ^GT[GC]AC XhoII 233 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### AlwNI 101 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG 307 CAGGCGCTG BanII 355 GAGCTC G[GA]GC[CT]^C 467 GGGCTC BbvCI 502 GCTGAGG CCTCAGC(-5/-2) 675 GCTGAGG BcnI 515 CCCGG CC^[GC]GG 842 CCGGG BmyI 355 GAGCTC G[AGT]GC[ACT]^C 467 GGGCTC BsaI 109 GAGACC GGTCTC(1/5) 118 GAGACC BsaWI 272 ACCGGA [AT]^CCGG[AT] 419 TCCGGA Bsp1286I 355 GAGCTC G[AGT]GC[ACT]^C 467 GGGCTC HaeII 309 GGCGCT [GA]GCGC^[CT] 845 GGCGCT HinfI 184 GAGTC G^A[ACGT]TC 621 GATTC MaeIII 557 GTTAC ^GT[ACGT]AC 838 GTCAC NciI 515 CCCGG CC^[GC]GG 842 CCGGG PvuII 56 CAGCTG CAG^CTG 673 CAGCTG ######################################### # 3 OR MORE CUT ENZYMES ( 46 enzymes) # ######################################### AciI AluI AlwI BbvI BsaJI BseRI BsiYI BslI BsmAI BsrBI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII EarI EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII ItaI Ksp632I MboI MboII MnlI MspA1I MspI MvaI MwoI NdeII NlaIII Sau3AI Sau96I ScrFI SfaNI TaqI TseI TthHB8I