(Circular) (Six-base) MAPSORT of: pFN21AE5316.seq Check: 1302 from: 1 to: 5560 pFN21AE5316 5560 bp With 182 enzymes: SgfI * January 30, 2014 17:17 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2395 3370 278 Size: 53 83 186 936 859 975 2468 Fragments arranged by size: 2468 975 936 859 186 83 53 AccI GT'mk_AC Cuts at: 1937 2903 1937 Size: 966 4594 Acc65I G'GTAC_C Cuts at: 2881 2881 Size: 5560 AclI AA'CG_TT Cuts at: 1563 3687 4060 1563 Size: 2124 373 3063 Fragments arranged by size: 3063 2124 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2121 2190 2484 3623 5007 5275 1632 Size: 489 69 294 1139 1384 268 1917 Fragments arranged by size: 1917 1384 1139 489 294 268 69 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5363 AflIII A'CryG_T Cuts at: 1203 4459 5436 1203 Size: 3256 977 1327 Fragments arranged by size: 3256 1327 977 AgeI A'CCGG_T Cuts at: 4391 4391 Size: 5560 AhdI GACnn_n'nnGTC Cuts at: 4289 4289 Size: 5560 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5560 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5560 AlwNI CAG_nnn'CTG Cuts at: 2111 2317 3303 4875 2111 Size: 206 986 1572 2796 Fragments arranged by size: 2796 1572 986 206 ApaLI G'TGCA_C Cuts at: 3617 4773 3617 Size: 1156 4404 ApoI r'AATT_y Cuts at: 1493 2869 3095 3247 5356 1493 Size: 1376 226 152 2109 1697 Fragments arranged by size: 2109 1697 1376 226 152 AseI AT'TA_AT Cuts at: 160 1032 4114 160 Size: 872 3082 1606 Fragments arranged by size: 3082 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5560 AvaI C'yCGr_G Cuts at: 1870 1943 2885 1870 Size: 73 942 4545 Fragments arranged by size: 4545 942 73 BamHI G'GATC_C Cuts at: 2890 3257 2890 Size: 367 5193 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2227 2881 4336 618 Size: 325 217 627 440 654 1455 1842 Fragments arranged by size: 1842 1455 654 627 440 325 217 BanII G_rGCy'C Cuts at: 1391 2364 2476 2879 1391 Size: 973 112 403 4072 Fragments arranged by size: 4072 973 403 112 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2579 928 Size: 971 680 3909 Fragments arranged by size: 3909 971 680 BbvCI CC'TCA_GC Cuts at: 2508 2681 2508 Size: 173 5387 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3751 3785 1903 Size: 34 1814 34 3678 Fragments arranged by size: 3678 1814 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3452 4662 3452 Size: 1210 4350 BclI T'GATC_A Cuts at: 1548 1548 Size: 5560 BfrBI ATG'CAT Cuts at: 2551 2551 Size: 5560 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4171 136 Size: 107 122 71 631 605 2499 1525 Fragments arranged by size: 2499 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5555 5555 Size: 5560 BlpI GC'TnA_GC Cuts at: 2977 2977 Size: 5560 Bme1580I G_kGCm'C Cuts at: 1790 3621 4777 1790 Size: 1831 1156 2573 Fragments arranged by size: 2573 1831 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5560 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3291 4249 442 Size: 416 53 83 399 24 1874 958 1753 Fragments arranged by size: 1874 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5560 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4220 1239 Size: 2981 2579 Bpu10I CC'TnA_GC Cuts at: 2508 2681 2508 Size: 173 5387 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3685 4550 4848 3685 Size: 865 298 4397 Fragments arranged by size: 4397 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2108 2117 4223 882 Size: 617 609 9 2106 2219 Fragments arranged by size: 2219 2106 617 609 9 BsaAI yAC'GTr Cuts at: 493 1204 3351 493 Size: 711 2147 2702 Fragments arranged by size: 2702 2147 711 BsaBI GATnn'nnATC Cuts at: 5554 5554 Size: 5560 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2392 3367 3749 4384 Size: 53 83 186 936 859 975 382 635 Cuts at: 4384 275 Size: 1451 Fragments arranged by size: 1451 975 936 859 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2277 2424 3993 4391 4665 4812 5223 2277 Size: 147 1569 398 274 147 411 2614 Fragments arranged by size: 2614 1569 411 398 274 147 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2241 2271 1178 Size: 30 165 30 838 30 4467 Fragments arranged by size: 4467 838 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2054 2057 2060 2093 2135 2201 2366 2369 Size: 868 3 3 33 42 66 165 3 Cuts at: 2369 2372 2498 2501 2504 2653 1186 Size: 3 126 3 3 149 4093 Fragments arranged by size: 4093 868 165 149 126 66 42 33 3 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 4763 5259 4763 Size: 496 5064 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4987 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3010 3771 4799 1540 Size: 275 186 1009 761 1028 2301 Fragments arranged by size: 2301 1028 1009 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2364 3621 3706 4777 2364 Size: 1257 85 1071 3147 Fragments arranged by size: 3147 1257 1071 85 BsmI GAATG_Cn' Cuts at: 3071 3164 3071 Size: 93 5467 BsmBI CGTCTCn'nnnn_ Cuts at: 2383 2383 Size: 5560 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2364 2476 2879 3621 3706 4777 1391 Size: 399 574 112 403 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 574 403 399 112 85 BspEI T'CCGG_A Cuts at: 2424 5223 2424 Size: 2799 2761 BspHI T'CATG_A Cuts at: 3449 5179 3449 Size: 1730 3830 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2751 2915 844 Size: 1907 164 3489 Fragments arranged by size: 3489 1907 164 BsrBI CCG'CTC Cuts at: 1394 1823 2404 2434 2446 2581 2608 3447 1394 Size: 429 581 30 12 135 27 839 3507 Fragments arranged by size: 3507 839 581 429 135 30 27 12 BsrDI GCAATG_nn' Cuts at: 65 1248 4055 4229 65 Size: 1183 2807 174 1396 Fragments arranged by size: 2807 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4204 4391 1700 Size: 110 2394 187 2869 Fragments arranged by size: 2869 2394 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5560 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5560 BssSI C'ACGA_G Cuts at: 3620 4632 3620 Size: 1012 4548 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5560 BstBI TT'CG_AA Cuts at: 4364 4364 Size: 5560 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5560 BstYI r'GATC_y Cuts at: 1972 2238 2890 3257 3642 3659 5111 5197 5209 Size: 266 652 367 385 17 1452 86 12 Cuts at: 5209 5555 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 652 385 367 346 266 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2827 513 Size: 552 1762 3246 Fragments arranged by size: 3246 1762 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4821 Fragments arranged by size: 4821 683 56 BtsI GCAGTG_nn' Cuts at: 3076 3870 3890 3076 Size: 794 20 4746 Fragments arranged by size: 4746 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5560 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5525 DraI TTT'AAA Cuts at: 2864 2864 Size: 5560 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4567 809 Size: 507 3251 1802 Fragments arranged by size: 3251 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2652 3007 3896 Size: 54 1425 50 275 18 822 355 889 Cuts at: 3896 5525 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 822 355 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3007 1537 Size: 275 1195 4090 Fragments arranged by size: 4090 1195 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2013 2025 2064 2085 2145 2460 2588 3490 Size: 660 12 39 21 60 315 128 902 Cuts at: 3490 1353 Size: 3423 Fragments arranged by size: 3423 902 660 315 128 60 39 21 12 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4147 4521 4667 387 Size: 1064 138 2558 374 146 1280 Fragments arranged by size: 2558 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2362 2362 Size: 5560 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2121 2190 2484 3623 4220 5007 5275 Size: 393 489 69 294 1139 597 787 268 Cuts at: 5275 1239 Size: 1524 Fragments arranged by size: 1524 1139 787 597 489 393 294 268 69 EcoO109I rG'GnC_Cy Cuts at: 2296 2296 Size: 5560 EcoRI G'AATT_C Cuts at: 2869 3247 2869 Size: 378 5182 FspI TGC'GCA Cuts at: 4066 4066 Size: 5560 HaeII r_GCGC'y Cuts at: 1678 2318 2854 4707 1678 Size: 640 536 1853 2531 Fragments arranged by size: 2531 1853 640 536 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2311 2343 4201 4233 4275 Size: 32 135 32 739 32 1858 32 42 Cuts at: 4275 4307 1373 Size: 32 2626 Fragments arranged by size: 2626 1858 739 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2904 3150 669 Size: 988 281 966 246 3079 Fragments arranged by size: 3079 988 966 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5560 HpaI GTT'AAC Cuts at: 3150 3150 Size: 5560 KpnI G_GTAC'C Cuts at: 2885 2885 Size: 5560 MfeI C'AATT_G Cuts at: 3159 3159 Size: 5560 MluI A'CGCG_T Cuts at: 5436 5436 Size: 5560 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2565 4674 4858 1305 Size: 1260 2109 184 2007 Fragments arranged by size: 2109 2007 1260 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2654 10 Size: 54 1425 343 822 2916 Fragments arranged by size: 2916 1425 822 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2667 3518 3877 4036 518 Size: 648 1501 851 359 159 2042 Fragments arranged by size: 2042 1501 851 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2063 2087 2680 2829 2976 3653 4801 5046 Size: 457 24 593 149 147 677 1148 245 Cuts at: 5046 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 593 457 245 149 147 24 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5008 NdeI CA'TA_TG Cuts at: 387 387 Size: 5560 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5560 NotI GC'GGCC_GC Cuts at: 3007 3007 Size: 5560 NsiI A_TGCA'T Cuts at: 2553 2553 Size: 5560 NspI r_CATG'y Cuts at: 1132 1229 2918 4463 1132 Size: 97 1689 1545 2229 Fragments arranged by size: 2229 1689 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5560 PciI A'CATG_T Cuts at: 4459 4459 Size: 5560 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5560 PmeI GTTT'AAAC Cuts at: 2864 2864 Size: 5560 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3629 3661 5142 5174 3629 Size: 32 1481 32 4015 Fragments arranged by size: 4015 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2296 2296 Size: 5560 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5560 PsiI TTA'TAA Cuts at: 3130 3130 Size: 5560 PstI C_TGCA'G Cuts at: 830 2748 2912 830 Size: 1918 164 3478 Fragments arranged by size: 3478 1918 164 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5560 PvuII CAG'CTG Cuts at: 2063 2680 2063 Size: 617 4943 SacI G_AGCT'C Cuts at: 2364 2364 Size: 5560 SacII CC_GC'GG Cuts at: 2830 2830 Size: 5560 SalI G'TCGA_C Cuts at: 1936 2902 1936 Size: 966 4594 SapI GCTCTTCn'nnn_ Cuts at: 2064 2064 Size: 5560 SbfI CC_TGCA'GG Cuts at: 2748 2912 2748 Size: 164 5396 ScaI AGT'ACT Cuts at: 3808 5520 3808 Size: 1712 3848 SfcI C'TryA_G Cuts at: 826 1045 2744 2908 4043 4724 4915 826 Size: 219 1699 164 1135 681 191 1471 Fragments arranged by size: 1699 1471 1135 681 219 191 164 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5560 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5560 SmaI CCC'GGG Cuts at: 1872 2887 1872 Size: 1015 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3664 4565 4827 820 Size: 197 926 1721 901 262 1553 Fragments arranged by size: 1721 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3351 493 Size: 2858 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5560 SphI G_CATG'C Cuts at: 1132 2918 1132 Size: 1786 3774 SspI AAT'ATT Cuts at: 5 52 3484 5 Size: 47 3432 2081 Fragments arranged by size: 3432 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5560 StyI C'CwwG_G Cuts at: 513 1065 2694 2999 513 Size: 552 1629 305 3074 Fragments arranged by size: 3074 1629 552 305 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2132 3587 3604 3757 3942 4387 325 Size: 1255 552 1455 17 153 185 445 1498 Fragments arranged by size: 1498 1455 1255 552 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3806 5518 96 Size: 274 80 33 51 1443 1829 1712 138 Fragments arranged by size: 1829 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2241 2273 5520 5552 2241 Size: 32 3247 32 2249 Fragments arranged by size: 3247 2249 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5560 XbaI T'CTAG_A Cuts at: 2896 2896 Size: 5560 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5560 XmaI C'CCGG_G Cuts at: 1870 2885 1870 Size: 1015 4545 XmnI GAAnn'nnTTC Cuts at: 3689 3689 Size: 5560 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2393 3368 276 Size: 53 83 186 936 859 975 2468 Fragments arranged by size: 2468 975 936 859 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SanDI SexAI SfiI SfoI SrfI SwaI XcmI