################################## # NO CUT ENZYMES ( 109 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AflIII AgeI AhdI Alw44I AocI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AviII BbrPI BbvCI BclI BfrI BglII BlpI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoO65I EcoRV EcoT22I FbaI FseI FspI HgaI HindIII HpaI KpnI KspI MamI MfeI MluI MroI MunI NcoI NotI NruI NsiI PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SnaBI SphI Sse8387I SspBI SwaI Van91I XhoI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### AccI 1623 GTCTAC GT^[AC][GT]AC AccII 273 CGCG CG^CG AcyI 210 GGCGCC G[GA]^CG[CT]C AlwNI 1592 CAGGTTCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 135 AGCGCT AGC^GCT ApaI 186 GGGCCC GGGCC^C Asp700I 571 GAACTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 1351 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 315 CCTAGG C^CTAGG BamHI 1572 GGATCC G^GATCC BanI 210 GGCGCC G^G[CT][GA]CC BbeI 210 GGCGCC GGCGC^C BbsI 1093 GAAGAC GAAGAC(2/6) BcgI 392 GCAACGACTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 549 GTATCC GTATCC(6/5) BglI 179 GCCTCGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 315 CCTAGG C^CTAGG BpuAI 1093 GAAGAC GAAGAC(2/6) BsaHI 210 GGCGCC G[GA]^CG[CT]C BsmI 1180 GAATGC GAATGC(1/-1) BspMI 1402 ACCTGC ACCTGC(4/8) BstBI 1410 TTCGAA TT^CGAA BstUI 273 CGCG CG^CG DraIII 625 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG Eco47III 135 AGCGCT AGC^GCT EcoRI 796 GAATTC G^AATTC Hin1I 210 GGCGCC G[GA]^CG[CT]C HincII 1555 GTTGAC GT[CT]^[GA]AC HindII 1555 GTTGAC GT[CT]^[GA]AC KasI 210 GGCGCC G^GCGCC MvnI 273 CGCG CG^CG NarI 210 GGCGCC GG^CGCC NdeI 1235 CATATG CA^TATG NheI 1459 GCTAGC G^CTAGC NspI 768 ACATGC [GA]CATG^[CT] NspV 1410 TTCGAA TT^CGAA PflFI 1351 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 2134 GAGTC GAGTC(4/5) PpuMI 49 GGGTCCT [GA]G^G[AT]CC[CT] PshAI 1508 GACAGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SfcI 781 CTACAG C^T[GA][CT]AG SfiI 178 GGCCTCGGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 210 GGCGCC GGC^GCC SfuI 1410 TTCGAA TT^CGAA SgrAI 288 CGCCGGCG C[GA]^CCGG[CT]G SmaI 46 CCCGGG CCC^GGG SmlI 802 CTCAAG C^T[CT][GA]AG SpeI 732 ACTAGT A^CTAGT SspI 535 AATATT AAT^ATT StuI 1938 AGGCCT AGG^CCT Tth111I 1351 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 1632 TCTAGA T^CTAGA XcmI 1700 CCAGATACCATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 46 CCCGGG C^CCGGG XmaI 46 CCCGGG C^CCGGG XmnI 571 GAACTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AvaII 50 GGTCC G^G[AT]CC 621 GGACC BsgI 736 GTGCAG GTGCAG(16/14) 1724 GTGCAG BsiYI 283 CCCAGCGCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 696 CCTTGATTTGG BslI 283 CCCAGCGCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 696 CCTTGATTTGG BsrFI 166 GCCGGC [GA]^CCGG[CT] 289 GCCGGC BstYI 842 AGATCC [GA]^GATC[CT] 1572 GGATCC Cfr10I 166 GCCGGC [GA]^CCGG[CT] 289 GCCGGC DraII 49 GGGTCCT [GA]G^G[ACGT]CC[CT] 185 GGGGCCC DsaI 116 CCGTGG C^C[GA][CT]GG 248 CCACGG EcoO109I 49 GGGTCCT [GA]G^G[ACGT]CC[CT] 185 GGGGCCC EcoT14I 315 CCTAGG C^C[AT][AT]GG 1039 CCTTGG MaeII 1054 ACGT A^CGT 1899 ACGT MflI 842 AGATCC [GA]^GATC[CT] 1572 GGATCC MslI 1741 CACTACTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1915 CATATCAATG NaeI 166 GCCGGC GCC^GGC 289 GCCGGC NgoMIV 166 GCCGGC G^CCGGC 289 GCCGGC PvuII 267 CAGCTG CAG^CTG 1419 CAGCTG StyI 315 CCTAGG C^C[AT][AT]GG 1039 CCTTGG Tsp45I 70 GTGAC ^GT[GC]AC 1298 GTGAC XhoII 842 AGATCC [GA]^GATC[CT] 1572 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 73 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI AvaI BalI BanII BbvI BcnI BfaI BmyI BpmI BsaJI BseRI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII EaeI EarI EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MluNI MnlI MscI MseI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I