(Circular) (Six-base) MAPSORT of: pFN21AB5728.seq Check: 5758 from: 1 to: 6898 pFN21AB5728 6898 bp With 182 enzymes: SgfI * March 28, 2012 12:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4708 278 Size: 53 83 186 936 3172 2468 Fragments arranged by size: 3172 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3629 4241 1937 Size: 1692 612 4594 Fragments arranged by size: 4594 1692 612 Acc65I G'GTAC_C Cuts at: 4219 4219 Size: 6898 AclI AA'CG_TT Cuts at: 1563 5025 5398 1563 Size: 3462 373 3063 Fragments arranged by size: 3462 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3579 4961 6345 6613 1632 Size: 1947 1382 1384 268 1917 Fragments arranged by size: 1947 1917 1384 1382 268 AfeI AGC'GCT Cuts at: 2142 2142 Size: 6898 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6701 AflIII A'CryG_T Cuts at: 1203 5797 6774 1203 Size: 4594 977 1327 Fragments arranged by size: 4594 1327 977 AgeI A'CCGG_T Cuts at: 5729 5729 Size: 6898 AhdI GACnn_n'nnGTC Cuts at: 5627 5627 Size: 6898 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6898 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6898 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2600 2632 2600 Size: 32 6866 AlwNI CAG_nnn'CTG Cuts at: 3602 4641 6213 3602 Size: 1039 1572 4287 Fragments arranged by size: 4287 1572 1039 ApaI G_GGCC'C Cuts at: 2195 2195 Size: 6898 ApaLI G'TGCA_C Cuts at: 4955 6111 4955 Size: 1156 5742 ApoI r'AATT_y Cuts at: 1493 2801 3176 3287 3689 4097 4207 4433 4585 Size: 1308 375 111 402 408 110 226 152 Cuts at: 4585 6694 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1308 408 402 375 226 152 111 110 AseI AT'TA_AT Cuts at: 160 1032 5452 160 Size: 872 4420 1606 Fragments arranged by size: 4420 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6898 AvaI C'yCGr_G Cuts at: 1870 1943 2051 2176 2186 4223 1870 Size: 73 108 125 10 2037 4545 Fragments arranged by size: 4545 2037 125 108 73 10 AvrII C'CTAG_G Cuts at: 2320 2320 Size: 6898 BamHI G'GATC_C Cuts at: 3577 4228 4595 3577 Size: 651 367 5880 Fragments arranged by size: 5880 651 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2215 4219 5674 618 Size: 325 217 627 428 2004 1455 1842 Fragments arranged by size: 2004 1842 1455 627 428 325 217 BanII G_rGCy'C Cuts at: 1391 2036 2195 2373 4217 1391 Size: 645 159 178 1844 4072 Fragments arranged by size: 4072 1844 645 178 159 BbeI G_GCGC'C Cuts at: 2219 2219 Size: 6898 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3105 928 Size: 971 1206 4721 Fragments arranged by size: 4721 1206 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2386 2420 5089 5123 1903 Size: 34 449 34 2669 34 3678 Fragments arranged by size: 3678 2669 449 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2565 4790 6000 2565 Size: 2225 1210 3463 Fragments arranged by size: 3463 2225 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6898 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2190 5509 136 Size: 107 122 71 631 605 518 3319 1525 Fragments arranged by size: 3319 1525 631 605 518 122 107 71 BglII A'GATC_T Cuts at: 6893 6893 Size: 6898 BlpI GC'TnA_GC Cuts at: 4315 4315 Size: 6898 Bme1580I G_kGCm'C Cuts at: 1790 2195 4126 4959 6115 1790 Size: 405 1931 833 1156 2573 Fragments arranged by size: 2573 1931 1156 833 405 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6898 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4629 5587 442 Size: 416 53 83 399 24 3212 958 1753 Fragments arranged by size: 3212 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3468 1056 Size: 2412 4486 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2299 2991 3342 4138 5558 1239 Size: 1060 692 351 796 1420 2579 Fragments arranged by size: 2579 1420 1060 796 692 351 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2792 5023 5888 6186 2792 Size: 2231 865 298 3504 Fragments arranged by size: 3504 2231 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5561 882 Size: 617 4062 2219 Fragments arranged by size: 4062 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4689 493 Size: 711 3485 2702 Fragments arranged by size: 3485 2702 711 BsaBI GATnn'nnATC Cuts at: 6892 6892 Size: 6898 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2216 4705 5087 5722 Size: 53 83 186 936 683 2489 382 635 Cuts at: 5722 275 Size: 1451 Fragments arranged by size: 2489 1451 936 683 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5331 5729 6003 6150 6561 5331 Size: 398 274 147 411 5668 Fragments arranged by size: 5668 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6673 Fragments arranged by size: 6673 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2059 2077 2339 1186 Size: 873 18 262 5745 Fragments arranged by size: 5745 873 262 18 BseYI C'CCAG_C Cuts at: 2039 2288 6101 6597 2039 Size: 249 3813 496 2340 Fragments arranged by size: 3813 2340 496 249 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2762 3750 1163 Size: 573 1026 988 4311 Fragments arranged by size: 4311 1026 988 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4348 5109 6137 1540 Size: 275 186 2347 761 1028 2301 Fragments arranged by size: 2347 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4959 5044 6115 4959 Size: 85 1071 5742 Fragments arranged by size: 5742 1071 85 BsmI GAATG_Cn' Cuts at: 3191 4409 4502 3191 Size: 1218 93 5587 Fragments arranged by size: 5587 1218 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2036 2195 2373 4126 4217 4959 5044 Size: 399 246 159 178 1753 91 742 85 Cuts at: 5044 6115 1391 Size: 1071 2174 Fragments arranged by size: 2174 1753 1071 742 399 246 178 159 91 85 BspEI T'CCGG_A Cuts at: 6561 6561 Size: 6898 BspHI T'CATG_A Cuts at: 4787 6517 4787 Size: 1730 5168 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3416 4253 844 Size: 2572 837 3489 Fragments arranged by size: 3489 2572 837 BsrBI CCG'CTC Cuts at: 1394 1823 4785 1394 Size: 429 2962 3507 Fragments arranged by size: 3507 2962 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5393 5567 65 Size: 1183 4145 174 1396 Fragments arranged by size: 4145 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2171 2294 5542 5729 1700 Size: 110 361 123 3248 187 2869 Fragments arranged by size: 3248 2869 361 187 123 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6898 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6898 BssSI C'ACGA_G Cuts at: 4958 5970 4958 Size: 1012 5886 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6898 BstBI TT'CG_AA Cuts at: 3416 5702 3416 Size: 2286 4612 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6898 BstYI r'GATC_y Cuts at: 1972 2847 3577 4228 4595 4980 4997 6449 6535 Size: 875 730 651 367 385 17 1452 86 Cuts at: 6535 6547 6893 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 875 730 651 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2121 2253 513 Size: 552 1056 132 5158 Fragments arranged by size: 5158 1056 552 132 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6159 Fragments arranged by size: 6159 683 56 BtsI GCAGTG_nn' Cuts at: 3116 3381 3475 4414 5208 5228 3116 Size: 265 94 939 794 20 4786 Fragments arranged by size: 4786 939 794 265 94 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6898 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2450 2485 529 Size: 35 1886 35 4942 Fragments arranged by size: 4942 1886 35 35 DraI TTT'AAA Cuts at: 4202 4202 Size: 6898 DraIII CAC_nnn'GTG Cuts at: 2635 2635 Size: 6898 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5905 809 Size: 507 4589 1802 Fragments arranged by size: 4589 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2280 2458 2650 Size: 54 1425 50 275 18 450 178 192 Cuts at: 2650 2709 4345 5234 6863 8 Size: 59 1636 889 1629 43 Fragments arranged by size: 1636 1629 1425 889 450 275 192 178 59 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4345 1537 Size: 275 2533 4090 Fragments arranged by size: 4090 2533 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2107 2868 3539 3706 4828 1353 Size: 754 761 671 167 1122 3423 Fragments arranged by size: 3423 1122 761 754 671 167 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2071 5485 5859 6005 387 Size: 1064 138 482 3414 374 146 1280 Fragments arranged by size: 3414 1280 1064 482 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2299 2991 3342 3579 4138 4961 5558 Size: 393 667 692 351 237 559 823 597 Cuts at: 5558 6345 6613 1239 Size: 787 268 1524 Fragments arranged by size: 1524 823 787 692 667 597 559 393 351 268 237 EcoO109I rG'GnC_Cy Cuts at: 2055 2191 2055 Size: 136 6762 EcoRI G'AATT_C Cuts at: 2801 4207 4585 2801 Size: 1406 378 5114 Fragments arranged by size: 5114 1406 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2717 2717 Size: 6898 FspI TGC'GCA Cuts at: 5404 5404 Size: 6898 HaeII r_GCGC'y Cuts at: 1678 2144 2219 2295 6045 1678 Size: 466 75 76 3750 2531 Fragments arranged by size: 3750 2531 466 76 75 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2334 2366 4124 4156 5539 Size: 32 135 32 762 32 1758 32 1383 Cuts at: 5539 5571 5613 5645 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1758 1383 762 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3562 4242 4488 669 Size: 988 281 1624 680 246 3079 Fragments arranged by size: 3079 1624 988 680 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6898 HpaI GTT'AAC Cuts at: 4488 4488 Size: 6898 KasI G'GCGC_C Cuts at: 2215 2215 Size: 6898 KpnI G_GTAC'C Cuts at: 4223 4223 Size: 6898 MfeI C'AATT_G Cuts at: 4497 4497 Size: 6898 MluI A'CGCG_T Cuts at: 6774 6774 Size: 6898 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2709 3150 3416 3533 6012 6196 1305 Size: 1404 441 266 117 2479 184 2007 Fragments arranged by size: 2479 2007 1404 441 266 184 117 MscI TGG'CCA Cuts at: 10 64 1489 1832 2460 2652 2711 10 Size: 54 1425 343 628 192 59 4197 Fragments arranged by size: 4197 1425 628 343 192 59 54 MslI CAynn'nnrTG Cuts at: 518 1166 3750 3924 4856 5215 5374 518 Size: 648 2584 174 932 359 159 2042 Fragments arranged by size: 2584 2042 932 648 359 174 159 MspA1I CmG'CkG Cuts at: 1606 2039 2274 2328 3426 4314 4991 6139 6384 Size: 433 235 54 1098 888 677 1148 245 Cuts at: 6384 1606 Size: 2120 Fragments arranged by size: 2120 1148 1098 888 677 433 245 235 54 NaeI GCC'GGC Cuts at: 2173 2296 2173 Size: 123 6775 NarI GG'CG_CC Cuts at: 2216 2216 Size: 6898 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6346 NdeI CA'TA_TG Cuts at: 387 3241 387 Size: 2854 4044 NgoMIV G'CCGG_C Cuts at: 2171 2294 2171 Size: 123 6775 NheI G'CTAG_C Cuts at: 1052 3464 1052 Size: 2412 4486 NotI GC'GGCC_GC Cuts at: 4345 4345 Size: 6898 NspI r_CATG'y Cuts at: 1132 1229 2777 4256 5801 1132 Size: 97 1548 1479 1545 2229 Fragments arranged by size: 2229 1548 1545 1479 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6898 PciI A'CATG_T Cuts at: 5797 5797 Size: 6898 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6898 PfoI T'CCnGG_A Cuts at: 3366 3366 Size: 6898 PmeI GTTT'AAAC Cuts at: 4202 4202 Size: 6898 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4967 4999 6480 6512 4967 Size: 32 1481 32 5353 Fragments arranged by size: 5353 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2055 2055 Size: 6898 PshAI GACnn'nnGTC Cuts at: 1613 3517 1613 Size: 1904 4994 PsiI TTA'TAA Cuts at: 4468 4468 Size: 6898 PspOMI G'GGCC_C Cuts at: 2191 2191 Size: 6898 PstI C_TGCA'G Cuts at: 830 4250 830 Size: 3420 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6898 PvuII CAG'CTG Cuts at: 2274 3426 2274 Size: 1152 5746 SalI G'TCGA_C Cuts at: 1936 4240 1936 Size: 2304 4594 SbfI CC_TGCA'GG Cuts at: 4250 4250 Size: 6898 ScaI AGT'ACT Cuts at: 5146 6858 5146 Size: 1712 5186 SfcI C'TryA_G Cuts at: 826 1045 2786 4246 5381 6062 6253 826 Size: 219 1741 1460 1135 681 191 1471 Fragments arranged by size: 1741 1471 1460 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2190 2190 Size: 6898 SfoI GGC'GCC Cuts at: 2217 2217 Size: 6898 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6898 SgrAI Cr'CCGG_yG Cuts at: 1700 2294 1700 Size: 594 6304 SmaI CCC'GGG Cuts at: 1872 2053 4225 1872 Size: 181 2172 4545 Fragments arranged by size: 4545 2172 181 SmlI C'TyrA_G Cuts at: 820 1017 1943 2807 5002 5903 6165 820 Size: 197 926 864 2195 901 262 1553 Fragments arranged by size: 2195 1553 926 901 864 262 197 SnaBI TAC'GTA Cuts at: 493 4689 493 Size: 4196 2702 SpeI A'CTAG_T Cuts at: 152 2737 152 Size: 2585 4313 SphI G_CATG'C Cuts at: 1132 4256 1132 Size: 3124 3774 SspI AAT'ATT Cuts at: 5 52 2542 4822 5 Size: 47 2490 2280 2081 Fragments arranged by size: 2490 2280 2081 47 StuI AGG'CCT Cuts at: 1517 3945 1517 Size: 2428 4470 StyI C'CwwG_G Cuts at: 513 1065 2320 3044 4337 513 Size: 552 1255 724 1293 3074 Fragments arranged by size: 3074 1293 1255 724 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4089 4925 4942 5095 5280 5725 325 Size: 1255 2509 836 17 153 185 445 1498 Fragments arranged by size: 2509 1498 1255 836 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4002 5144 6856 Size: 274 80 33 51 1443 2025 1142 1712 Cuts at: 6856 96 Size: 138 Fragments arranged by size: 2025 1712 1443 1142 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6858 6890 6858 Size: 32 6866 Tth111I GACn'n_nGTC Cuts at: 1316 3359 1316 Size: 2043 4855 XbaI T'CTAG_A Cuts at: 3637 4234 3637 Size: 597 6301 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3712 3712 Size: 6898 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6898 XmaI C'CCGG_G Cuts at: 1870 2051 4223 1870 Size: 181 2172 4545 Fragments arranged by size: 4545 2172 181 XmnI GAAnn'nnTTC Cuts at: 2580 5027 2580 Size: 2447 4451 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4706 276 Size: 53 83 186 936 3172 2468 Fragments arranged by size: 3172 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SrfI SwaI